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Biochemistry

405

Fall, 2011 Exam 2: VERSION A

November 14, 2011

BC405 Fall Quarter 2011 - Midterm Exam 2 - Version A Lecturer: Jim Hurley Date: November 14th, 2011 Instructions: 1. BE SURE TO PUT YOUR NAME, STUDENT NUMBER, AND EXAM VERSION (A OR B OR C, SEE ABOVE) ON THE ANSWER SHEET. 2. Bubble in the answers onto the scantron sheet. 3. No books or notes are to be used during the exam. 4. No calculators or any electronic devices are to be used during the exam. 5. The test consists of 27 questions 6. Each question has one correct answer. 7. Each question is worth the same number of points. 8. This is a 45 minute exam.

Biochemistry 405
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Fall, 2011 Exam 2: VERSION A

November 14, 2011

Which ONE of the following is a ketose with 6 carbons? a. Ribose. b. Glucose. c. Galactose. d. Fructose. e. Glyceraldehyde. The reactions catalyzed by Pyruvate dehydrogenase, Pyruvate decarboxylase and -Ketoglutarate dehydrogenase all use which one of the following to help catalyze the reaction? a. Lipoic acid. b. FADH2. c. Thiamine pyrophosphate. d. ATP. e. NADH. Which ONE of the following is true regarding the reaction that converts hemoglobin to HbA1c? a. The rate of the reaction is faster when there is a lot of free valine in the blood. b. The rate of the reaction depends on the concentration of sucrose in the blood. c. The rate of the reaction depends on the concentration of glucose in the blood. d. The final product has a cobalt ion in it. e. A slower than normal rate of HbA1c formation indicates that a person has diabetes. Which ONE of the following reactions is catalyzed by Lactase? a. Conversion of a non-reducing disaccharide to two reducing monosaccharides. b. Conversion of a non-reducing disaccharide to two non-reducing monosaccharides. c. Conversion of a reducing disaccharide to two non-reducing monosaccharides. d. Conversion of a reducing disaccharide to two reducing monosaccharides. e. Conversion of lactose into sucrose. When cells are starved for glucose the NADH/NAD+ ratio in the cytoplasm becomes very low. Which ONE of the following would be most likely to accumulate in the cytoplasm under these conditions? a. Lactate. b. Oxaloacetate. c. Fructose-1,6-bisphosphate. d. Malate. e. ATP.

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Biochemistry 405
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Fall, 2011 Exam 2: VERSION A

November 14, 2011

Which ONE of the following correctly describes the type of Glucose transporter and the type of Hexokinase in the cells in the pancreas? a. High affinity glucose transporter, high affinity hexokinase. b. Low affinity glucose transporter, high affinity hexokinase. c. Low affinity glucose transporter, low affinity hexokinase. d. High affinity glucose transporter, low affinity hexokinase. e. cells in the pancreas do not have glucose transporters. Which ONE of the following enzymes catalyzes a reaction that occurs during the payoff phase of glycolysis? a. Hexokinase. b. Pyruvate decarboxylase. c. Phosphoglycerate mutase. d. Phosphoglucose isomerase. e. Phosphofructokinase. Some people have Fructose Intolerance. They cannot eat food that has fructose in it without getting sick. They are OK eating starch or glycogen. Which ONE of the following is most likely to be the enzyme that is deficient in people with Fructose Intolerance? a. An enzyme that produces Fructose-1,6-bisphosphate. b. An enzyme that produces Fructose-2,6-bisphosphate. c. An enzyme that produces Dihydroxyacetone phosphate and Glyceraldehyde. d. An enzyme that produces Fructose-6-phosphate. e. An enzyme that produces Dihydroxyacetone phosphate and Glyceraldehyde-3-phosphate. Which ONE of the following is an intermediate in the Investment phase of glycolysis that starts with glucose? a. Phosphoenolpyruvate b. 3-Bisphosphoglycerate. c. Fructose-1-phosphate. d. Glucose-1-phosphate e. Fructose-6-phosphate. Which ONE of the following metabolites will accumulate in the cytoplasm of a cell in which pyruvate kinase activity is abnormally decreased? a. Alanine. b. Citrate. c. Phosphoenolpyruvate. d. Pyruvate. e. Lactate. Which ONE of the following enzymes catalyzes a reaction in which Coenzyme A is a substrate and is part of the final product of the reaction? a. -Ketoglutarate dehydrogenase. b. Isocitrate dehydrogenase. c. Malate dehydrogenase. d. Pyruvate kinase. e. Pyruvate dehydrogenase kinase.

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Biochemistry 405

Fall, 2011 Exam 2: VERSION A

November 14, 2011

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Which ONE of the following reactions catalyzes the phosphorylation of GDP to GTP? a. A reaction that uses FAD as one of its substrates. b. A reaction that occurs in the payoff phase of glycolysis. c. A reaction that occurs within the phospholipid environment of the mitochondrial inner membrane. d. A reaction that transports NADH into mitochondria. e. A reaction that produces Coenzyme A as one of its products. Which ONE of the following TYPES of enzyme or transporter could move lactate out of a cell through the plasma membrane? a. An Aminotransferase. b. A Dehydrogenase. c. A Monocarboxylate transporter. d. A Tricarboxylic acid transporter. e. A Decarboxylase. Which ONE of the following enzymes involved in the Krebs Cycle produces a tricarboxylic acid as one of the products of the reaction it catalyzes? a. Pyruvate Dehydrogenase. b. -Ketoglutarate Dehydrogenase. c. Malate dehydrogenase. d. Succinate Dehydrogenase. e. Citrate Synthase. Which ONE of the following stimulates an increase of 2,3Bisphosphoglycerate in erythrocytes? a. Increased oxygen in the atmosphere. b. A decrease in the concentration of HCO3- in the blood. c. An increase in the concentration of lactic acid in the blood. d. A deficiency of hexokinase activity. e. A deficiency of phosphofructokinase-1. Methanol is metabolized in humans by the same enzymes that metabolize ethanol. Methanol is converted to formaldehyde by Alcohol dehydrogenase, then the formaldehyde is converted to formic acid by Aldehyde dehydrogenase. Formic acid is an inhibitor of cytochrome oxidase. It blocks respiration and causes depression of neuronal activity. In extreme cases of methanol poisoning the accumulation of formic acid causes unconsciousness and death. Which ONE of the following would be the most effective treatment for methanol poisoning? a. Pyruvate. b. Cyanide. c. Water. d. Ethanol. e. Quinone.

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Biochemistry 405
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Fall, 2011 Exam 2: VERSION A

November 14, 2011

Hydroxylation of a proline on HIF-1 decreases the rate of transcription of all of the following genes except one. Which ONE? a. Pyruvate Dehydrogenase E2. b. Pyruvate Dehydrogenase Kinase. c. Hexokinase. d. Aldolase. e. Pyruvate Kinase. Which ONE of the following molecules (a-d) is most oxidized? Or are all of the choices (a-d) in the same oxidation state (e). a. Formaldehyde. b. Methanol. c. Carbon Dioxide. d. Methane. e. All of the choices (a-d) are in the same oxidation state. Which ONE the following statements about Glycolysis is true? a. It occurs only in the space between the inner and outer mitochondrial membranes. b. Enolase uses NAD+ as a cofactor. c. Fructose 1,6 Bisphosphate is an allosteric inhibitor of Pyruvate kinase. d. Glyceraldehyde-3-phosphate dehydrogenase uses Thiamine pyrophosphate as a cofactor. e. When at equilibrium there is much more Dihydroxyacetone phosphate than Glyceraldehyde-3-phosphate in the reaction catalyzed by Triose phosphate isomerase. In the figure to the right, which ONE of the following choices is a pair of epimers? a. A and B b. B and C c. C and D d. A and D e. A and C In the same figure, which ONE of the following choices includes L-glucose and Dglucose? a. A and B b. B and C c. C and D d. A and D e. B and D

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Biochemistry 405

Fall, 2011 Exam 2: VERSION A

November 14, 2011

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Which ONE of the following (a-d) correctly describes the relationship between ATP and Phosphofructokinase I (PFK-1)? Or do none of the choices (a-d) correctly describe the relationship? a. ATP is both a substrate and an allosteric regulator of Phosphofructokinase I. b. ATP is an allosteric activator of PFK-1. c. ATP donates a phosphate to Fructose-1-phosphate to make F-1,6 bisphosphate in the reaction catalyzed by PFK-1. d. ATP donates a phosphate to AMP to counteract the regulatory effect of AMP on PFK-1. e. None of the choices (a-d) correctly describe the relationship. Which ONE of the following (a-d) correctly describes the influence of Fructose-1,6-bisphosphate (F1,6BP) on Pyruvate kinase activity? Or do none of the choices (a-d) correctly describe its influence? a. F1,6BP inhibits Pyruvate kinase activity. b. F1,6BP stimulates the conversion of Alanine to Pyruvate. c. F1,6BP competes with Phosphoenolpyruvate as a substrate for Pyruvate Kinase. d. The effect of F1,6BP on Pyruvate Kinase is opposite to the effect of Alanine on Pyruvate kinase. e. None of the choices (a-d) correctly describe its influence. The common ink cap mushroom (Coprinopsis atramentaria) is not toxic when eaten by itself, but when it is consumed with ethanol it causes facial reddening, nausea and vomiting. A chemical, coprine, in this mushroom produces a metabolite that inhibits an enzyme in the liver. Which ONE of the following options is most likely to be that enzyme? a. Pyruvate dehydrogenase. b. Aldehyde dehydrogenase. c. Monocarboxylate transporter. d. Lactate dehydrogenase. e. Pyruvate carboxylase. Each of these enzymes makes products. Which ONE makes a product that yields the greatest free energy change when phosphate is hydrolyzed from that product under standard conditions? a. ATP synthase. b. Phosphoglycerate kinase. c. Enolase. d. Hexokinase. e. Pyruvate kinase. Which ONE of the following enzymes or transport mechanisms helps to get reducing power from cytoplasmic NADH into mitochondria? a. Malate-aspartate shuttle. b. Pyruvate aminotransferase. c. Monocarboxylate transporter. d. Tricarboxylic acid transporter. e. Pyruvate carboxylase.

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Biochemistry 405

Fall, 2011 Exam 2: VERSION A

November 14, 2011

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Which ONE of the following statements (a-d) about the F1F0 ATP synthase molecule is correct? Or are none of the other statements correct (e)? a. The F1 subunit projects from the inner membrane into the intermembrane space between the inner and outer membranes of the mitochondria. b. F1F0 ATP synthase uses a transmembrane proton gradient to drive conformational changes in the heterodimer of the F1 subunit c. The F0 subunit binds ATP. d. Protons flow from the mitochondrial matrix through the F1F0 ATP synthase to the intermembrane space to generate ATP e. None of the other statements (a-d) is correct.