You are on page 1of 2

Kinetic-spectrophotometric method for the determination of ascorbic acid in orange juice, parsley and potatoes Link:!

Miltiades I. Karayannis and Despina I. Farasoglou Analyst, 1987,112, 767-770 DOI: 10.1039/AN9871200767

A kinetic-spectrophotometric method using stopped-flow techniques is described for the determination of ascorbic acid based on its reaction with 2,6-dichlorophenolindophenol (DCPl). The initial rate was measured with a specially designed system, which consists of an operational amplifier, a window comparator and a scaler which counts the pulses from a 1 MHz square-signal generator in order to measure the time interval, t, between two pre-set levels, E1and E2, of the electrical signal. The regression curve for the results is (t)1=(0.4 0.1)(162240 541)[ascorbic acid] with a correlation coefficient r=0.99988. It was found that 4 105M DCPl gives the best stability, reproducibility and linearity of the calibration graph. The optimum oxalic acid concentration is 0.2 M. Ascorbic acid can be quickly determined in samples of orange juice, parsley and potato in the range 2 10 55 104M. Recoveries of 98.0, 95.0 and 90.0% were obtained for orange juice, parsley and potato, respectively. The standard additions method gave recoveries of 101.7, 100.1 and 100.2% for orange juices, parsley and potato, respectively. The results obtained have been compared with the official method. The accuracy and reproducibility of the method are better than 2%. The concentrations of ascorbic acid in orange juices from four different areas of Greece are also presented.

Sequential injection technique for automated titration: Spectrophotometric assay of vitamin C in pharmaceutical products using cerium(IV) in sulfuric acid Link:
Salah M. Sultan , Yousif A. M. Hassan and K. E. E. Ibrahim Analyst, 1999,124, 917-921 DOI: 10.1039/A902000D

For the first time sequential injection analysis (SIA) technique has been employed for titrimetry. A new SI titrimetric spectrophotometric method for the assay of vitamin C in drug formulations was explored. The method is based on the oxidation reaction of vitamin C with cerium(IV) in sulfuric acid media using a specrophotometer as a detector with the wavelength monitored at 410 nm. A 23 factorial design chemometric approach was employed to study the interaction effect of the chemical and system variables, mainly cerium(IV), sulfuric acid concentrations and the flow rate. The results of the chemometric optimization revealed that the optimum operating conditions for the SI titrimetric analysis of vitamin C were 7.0 103 M cerium(IV), 0.455 M sulfuric acid and 28.9 L s1 flow rate. A linear calibration plot for the determination of vitamin C was obtained in the concentration range between 30 to 200 ppm. The method was applied to the determination of vitamin C in pharmaceutical preparations and no excipient was found to pose any interference, thus rendering the method suitable for the determination of the drug in pharmaceutical preparations. The SIA method is found to be accurate when the results were statistically compared with the results obtained by the BP standard method. The SIA method is superior when compared to the conventional titration method, the BP standard method and previous methods with respect to precision and automation in solution handling.

Vitamin C Its Chemistry and Biochemistry

Author(s): M B Davies, D A Partridge, J A Austin Copyright: 1991 ISBN: 978-1-84755-230-3 DOI:10.1039/9781847552303 Description Vitamin C is the first book to cover the history, chemistry, biochemistry, and medical importance of vitamin C and is the first to provide an in-depth, interdisciplinary study of this essential and fascinating compound. The book provides a comprehensive and systematic account of the vitamin C story, fully surveying the history of scurvy and how its cure led to the suggestion, discovery, and isolation of the vitamin, later named L-ascorbic acid. It describes in detail the vitamin's structure determination, synthesis and manufacture, and its oxidation products, derivatives and related compounds. Its key biochemical roles are fully categorized and explained, and the medical importance of the vitamin, including the recent use of so-called megadoses, is thoroughly discussed. Vitamin C will be of interest to a very wide readership and will provide useful background information and inspiration for students at various levels. It will also be relevant to the interested chemist or lay person, as well as those carrying out research in this area

Determination of vitamin C in chemical, pharmaceutical and biological samples by spectrophotometric titrimetry with o-diacetoxyiodobenzoate. Analysis of mixtures of vitamin C with methionine and cysteine or glutathione Link:!divAbstract
Ashutosh Srivastava and Sunil Kumar Singh Analyst, 1988,113, 259-262

DOI: 10.1039/AN9881300259

A method has been developed for the determination of vitamin C in pharmaceutical preparations, foodstuffs and biological fluids based on titrimetry with o-diacetoxyiodobenzoate (o-DIB) with spectrophotometric (max. 600 nm) or visual detection of the end-point usingleuco-2,6-dichlorophenolindophenol and potassium iodide as indicator. Sulphur-containing amino acids such as cysteine and glutathione interfere quantitatively and can be masked by cyanoethylation; the cyanoethylated product and methionine have also been determined with o-DIB in the presence of acidified potassium bromide with methyl red as indicator. Procedures are given for the analysis of mixtures of vitamin C with sulphur-containing amino acids. The effect of a number of interferents has also been studied.