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HPLC (high performance liquid chromatography)
Uses inert gas Can detect smaller molecules that can combine with gas Compound mixture are seperated using the liquid (stationary) phase and a gas (mobile) phase Has a larger diffusion coefficient of the sample in the mobile phase
Medium used Size that can be detected
Uses liquid Can detect larger molecules that dissolves into liquid
Stationary phase and mobile phase Diffusion coefficient of sample in mobile phase
Compound of a mixture are seperated using solid (stationary) phase and liquid (mobile) phase Has a smaller diffusion coefficient of the sample in mobile phase
Comparison between GC and HPLC Low Used to detect substances in atmosphere such as types of smoke. A computer changes the temperature while samples are analyzed. gases (volatile compounds) have coiled capillary columns with interior walls coated with various materials depending on the lab's needs GC columns are housed in an oven within the machine. The higher the temperature. Temperature Detectors Uses an UV-Vis. but temperatures that are too high produce poor results Uses FID universal to organic compounds Viscosity of mobile phase Function high Used to detect substances in liquid and solid (non volatile compounds) 2 Column Have typically four-to-six inch-long metal or glass tubes tightly packed with silica or differing carbon chain lengths. the faster the sample elutes. wide range of non destructive detectors . HPLC columns are kept at a stable temperature (most often room temperature) at all times.