Running head: MYSTERY ORGANISM

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Unit 6 Exercise 1 Mystery Organism Jilian McGugan ITT Technical Institute

GE150 Nelsen May 3, 2012

4) Protozoa are unicellular. morphology. Bergey’s Manual of Determinative Bacteriology has been a widely used reference for identifying bacteria based on cell wall composition. we have created a step by step guide to assist you in classifying the organism. 6) Viruses are not cellular (acellular). With the information provided.MYSTERY ORGANISM MEMO FROM: Jilian McGugan. Bergey’s Manual of Determinative Bacteriology is recommended to identify the organism. we know this microscopic organism is clonal and single-celled. Bacteria are relatively simple single-celled organisms. 2) Archaea are not known to cause human disease. oxygen requirements. . and shape. Staff Biologist TO: Carrie Johnson RE: Bacteria 2 All organisms can be grouped into a series of subdivisions that make up the taxonomic hierarchy. identify the Microorganism: Types of Microorganism: 1) Bacteria are relatively simple single-celled (unicellular) organisms. Important steps in the identification of organisms are to determine their size. Step 1. 3) Fungi can be unicellular or multicellular. With the clues provided. Since we have established that we are dealing with bacteria. differential staining. and biochemical testing. 5) Algae are unicellular.

6) Viruses are not cellular (acellular).MYSTERY ORGANISM Step 2. 3 . clusters. Can be unicellular or multicellular. the walls lack peptidoglycan. consist of prokaryotic cells. Characteristics to Observe 1) Bacteria are relatively simple single-celled (unicellular) organisms. Have a variety of shapes. or other groupings. bacillus (rod-like). eukaryotic microbes. Not known to cause human disease. but if they have cell walls. organisms whose cells have a distinct nucleus containing the cells genetic material (DNA). surrounded by a special envelope called the nuclear membrane. 2) Archaea are like bacteria. Appear in several shapes. Bacteria grown in a given time in media are called a culture. such formations are usually characteristic of a particular genus or species of bacteria. 5) Algae are unicellular with a variety of shapes. a population of cells from a single parent. A pure culture is often a clone. 3) Fungi are eukaryotic. coccus (spherical or ovoid). 4) Protozoa are unicellular. Individual bacteria may form pairs. Not enclosed in a special nuclear membrane. spiral (corkscrew or curved) star-shaped or square. chains.

4 Microorganism Clonal Bacteria Single-celled Protozoa Algae Fungi Bacteria Step 4. which will appear purple. Staphylococcus aureus is an example of Gram positive. Bergey’s Manual of Determinative Bacteriology is recommended for identifying unknown organisms. a Gram stain needs to be performed on the isolated sample. In order to be certain the organism is bacteria. Escherichia coli will appear red. .MYSTERY ORGANISM Step 3. Most bacteria are gram positive or gram negative. Identify the unknown microorganism. which is an example of Gram negative. Characteristics used to classify the organism. Gram staining is the most effective way to identify bacteria.

.. Microbiology an introduction. L. Hoboken: John Wiley & Sons. & Case. J.. (2007).MYSTERY ORGANISM References 5 Tortora. C. Trefil. San Francisco: Pearson Education. G. (2007). & Hazen. R. Inc. The Sciences. Funke. . J. B. R.

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