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Ari S. Akerstein, M.S.

AriAkerstein@gmail.com 3860 26th st, #4, SF CA 94131 415.902.6171

SUMMARY:

Molecular Genetics focused research with experience in cell culture. Bring a wide breadth of knowledge with eagerness to expand current skills, learn new experimental methods and contribute to a team environment.

SUMMARY OF ACCOMPLISHMENTS: • Recognized as the first SFSU graduate student to conduct thesis research in both the Molecular Biology and Mathematics departments • Master’s thesis validated a novel algorithm characterizing protein kinetics involved in radiation treatment in cancer cells • Awarded patent, successfully negotiated $600,000 licensing agreement for Akrowheels fitness product RESEARCH EXPERIENCE: 9/07-8/08 Molecular / Computational Biology Researcher San Francisco State University Department of Cell & Molecular Biology, Mathematics • Customized a Master’s program with research in both the Cell / Molecular Biology, Mathematics departments • Implemented Monte-Carlo simulations to analyze and image protein kinetics, outperforming standard methods • Validated algorithm performance by comparison to previously published data sets using advanced Excel analytics, Matlab • Prepared and presented talks, posters at professional scientific conferences, lab meetings and journal clubs • Thesis title: Simulating DNA repair mechanisms using stochastic, reactiondiffusion methods 10/07-08/08 Volunteer Laboratory Researcher University of California San Francisco (UCSF) Department of Orthopedic Surgery (Lotz Tissue Engineering Lab) • Prepared media / reagents for experiments and cell culture • Collaborated with Post Doctoral fellow to develop ultrasound techniques inducing stem cell differentiation • Developed strong sterile technique, good lab practices • Assisted development of three dimensional cell matrices to induce stem cells to cartilage regeneration • Performed RNA extraction & isolation, cell-count assays, RT-PCR analysis as well as cell culture passaging Post-Baccalaureate Laboratory Researcher San Francisco State University Department of Cell/Molecular Biology (Fuse Lab) • Conducted Experiments to elucidate endocrine mechanisms behind molting • Performed ICC/ICH staining, microscopy to determine hormone levels and

09/06-09/07

signaling during various larval stages of development • Performed patch clamp electrophysiology experiments on motor neurons to determine signaling patterns • Performed various dissections for electrophysiology experiments PUBLICATIONS / PRESENTATIONS • Akerstein, A., Bernstein, D. and Arsuaga, J. An in silico approach to analyzing nuclear FRAP protein recovery using an extension of the Gillespie algorithm, (In Press) Biochemistry and Cell Biology (2009) • Akerstein, A., Bernstein, D. and Arsuaga, J.Using the Gillespie Algorithm to investigate the role of chromatin geometry in the recruitment of repair proteins to DNA double strand breaks. Abstract, Biochemistry and Cell Biology, (2007) • Akerstein, A., Bernstein, D., and Arsuaga, J., Analysis of FRAP using an extension of the Gillespie algorithm. Abstract, Biochemistry and Cell Biology, (2006) • Presenter, 28th annual Asilomar Chromatin & Chromosomes Conference (2006), Using reaction-diffusion methods to simulate gamma-H2AX signaling of repair proteins in response to DNA double strand breaks SKILLS: LABORATORY Patch Clamp Electrophysiology, Cell Culture (stem cells: Human, Murine), Gel Electrophoresis, RNA extraction & isolation, Immunoc staining, small animal dissection, sterile technique, media preparation, cell viability assay, RT-PCR analysis, Analysis of Fluorescence Recovery after Photobleach (FRAP), Extremely organized documentation & lab notebook COMPUTATION / ANALYSIS MS Office, Excel, SAS (modeling, data analysis), Word, PowerPoint, Matlab, gene annotation (using Apollo), Expert analyzing data using Excel, NCBI BLAST, WU BLAST, Database queries for bioinformatics (e.g. GenBank, Flybase), Phylogenetic modeling, Unix, Matlab, Strong problem solving ability, Strong writing and interpersonal skill EDUCATION: San Francisco State University, San Francisco, CA MS, Molecular Biology, Aug 2008 Tested and validated a novel algorithm characterizing protein kinetics fundamental to DNA repair in irradiated cells for Master’s Thesis. Customized a Master’s program with research in both the Cell / Molecular Biology and Mathematics departments Boston University, Boston, MA, BA, International Relations, concentration in Economics July 2000 PROFESSIONAL SOCIETIES AAAS, K-stars marathon club, SF Debate, Long Now Foundation REFERENCES: Available upon request