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TECHNICAL DATA SHEET#1009 REV.

INDUSTRIAL APPLICATIONS
PRODUCT DESCRIPTIONS:
Bottled Media: B8445 (225 mL), B8457 (90 mL, wide mouth), B8458 (100 mL, wide mouth), B8460 (500 mL, Boston Round)

LACTOSE BROTH

PURPOSE:

Lactose Broth is used for the detection of lactose-fermenting, Gram-negative coliforms, as a pre-enrichment broth for Salmonella species, and in the study of lactose fermentation of bacteria in general. It is used extensively in testing water, foods, dairy and pharmaceutical products. Sublethally injured salmonellae may be present in low numbers in dried or processed foods. The recovery of Salmonella may be hindered by the presence of other bacteria as well as components of the food sample. Lactose Broth, a nonselective medium, supplies a pre-enriched environment, which allows for repair of cell damage while diluting toxic or inhibitory substances, and providing nutritional advantage to Salmonella over other bacteria.

PRINCIPLE:

Beef Extract and Peptone provide carbon and nitrogen sources for general growth requirements, while lactose provides a carbohydrate source. This pre-enrichment medium is used to provide a higher ratio of Salmonella to non-Salmonella bacteria after incubation. Lactose is fermented by most most non-Salmonella bacteria while Salmonella does not. A bactiostatic effect on competing microorganisms is created by the decrease of pH in the medium produced by the lactose fermenting bacteria. The Salmonella will have little growth during the initial 24 hours of incubation, while the non-Salmonella will be utlizing the lactose. As the products of fermentation build, the non-Salmonella are inhibited and the Salmonella begins its growth phase (24-48 hrs.)

FORMULAS:
Approximate, per liter of deionized filtered water. (1) Lactose Broth Enzymatic Digest of Gelatin.............................. 5.0 g Beef Extract....................................................... 3.0 g Lactose............................................................. 5.0 g Final pH: 6.9 + 0.2 at 25o C

PRECAUTIONS:*

For laboratory use only. Observe approved biohazard precautions. Storage: Upon receipt store at 225C. Media should not be used if there are signs of contamination, deterioration ( discoloration), or if the expiration date has passed. Media can be inoculated up to the expiration date and incubated for the appropriate incubation period. Limitations: Due to nutritional variation, some strains may grow poorly or fail to grow in this medium

PROCEDURE:*
Method of Use: Lactose Broth is used for the pre-enrichment phase for the testing of food and other materials, to enhance the recovery of Salmonella. Consult the appropriate references for specific procedures for each type of material being tested.1-4,6 1. Transfer a 25 g or 25 ml sample of test material into a container. Add 225 ml of sterile Lactose Broth. Mix as necessary to make a homogeneous suspension. Incubate at 35oC for 24 hours.

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TECHNICAL DATA SHEET#1009 REV. 2


2. Transfer 1 ml of suspension to appropriate enrichment broths such as Tetrathionate Broth and Selenite Cystine Broth.
Incubate at 35oC for 24 hours. 3. Transfer a loopful of suspension to appropriate selective agar media, such as Hektown Enteric Agar, XLD Agar and Bismuth Sulfite Agar. Incubate at 35oC for 24 hours Material Required but Not Provided: Standard microbiological supplies and equipment such as loops, needles, incubator, and incinerator are not provided.

QUALITY CONTROL:

Microorganism Enterococcus faecalis Escherichia coli Pseudomonas aeruginosa

ATCC # 29212 18739 9027

Response Good Growth Good Growth Good Growth

User Quality Control: Check for signs of contamination and deterioration.

BIBLIOGRAPHY:
1. Downes, F. P., Ito, K. (eds.), Compendium of Methods for the Microbiological Examination of Foods, 4th ed., American Public Health Association, Washington D. C., 2001. 2. Marshall, R. T. (ed.), Standard Mthods for the examination of Dairy Products, 16th ed. American Public Health Association, Washington, D.C. 3. U.S. Food and Drug Administration, Bacteriologicl Analytical Manual, 8th ed., AOAC International, Gaithersburg, MD., 1995. 4. Cunnif, P. (ed.), Official Methods of Analysis AOAC International, 16th ed. AOAC International, Gaithersburg, MD.,1995. 5. Franson, M. A. H. (ed.), Standard Methods for the Examination of Water and Wastewater, 20th ed., American Public Health Association, Washington D. C., 1998 6. U.S. Pharmacopeia and National Formulary, USP 27, NF 24, The USP convention Inc., Rockville, MD 2004. *For more detailed information, consult appropriate references.

PML MICROBIOLOGICALS, INC. Data #1009 Copyright 2005 by PML Microbiologicals, Inc. Revision Date: 06/20/2007

27120 SW 95th Avenue Wilsonville, OR 97070 800.547.0659 Page