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Poster Discussion 2 group, but there was no change of body weight. The IS.1was significantly increased in only the GH-treated group and it was negatively correlated with the ratio of the VFAfI’MA. The serum glucose levels, free fatty acid, fibrinogen, plasminogen activator inhibitor-l and HbAlc were significantly decreased after GH treatment. Total cholesterol and triglyceride were decreased in both groups. LDL-cholesterol was decreased in only the GH-treated group. The insulin-like growth factor-I, fasting c-peptide and insulin level was significantly increased after the GH treatment. This study suggested that in obese NIDDM GH treatment exerted a decrease in visceral fat and an increase in muscle mass via accelerated lipolytic and anabolic effects which could result in the improvement of

each 10 patients that were medicated with oral hypoglucemiants stopped using them after the treatment with autohemotherapy with a p < 0.05. Conclusion. The autohemotherapy treatment represents a new, effective method for treatment of Diabetes Mellittus Type 2, through reduction and normalization of the plasma glucose levels, and reduction of the use of oral hypoglucemiants, as well.

P954 Chronic Effect of Pferospermum Acerijolium Bark on Glycemic and Lipidemic Status of Type 2 Diabetic Model Rats S. MURSHED’, B. Rokeya’, L. Ali’, N. Nahar*, A.K. Azad Khan’, M. Mosihuzzaman 2. I Research Division, Birdem, Dhaka, Bangladesh:
2 Dept of Chemistry, Dhaha University, Dhaka, Bangladesh

plant in the Indian subcontinent, but its hypoglycemic effect has not yet been scientifically studied. Recently we found antihyperglycemic activity in the leaves of this plant in type 2 diabetic model rats. A subfraction (E4b) of the ethanol extract of the bark of the plant has now been tested both for its acute and chronic effects on glycemic status as well as on lipid levels in the same model. E4b (1.25 g/kg body weight) was fed daily to rats for 28 days. The Control rats were fed only with water. For acute experiment, blood samples were collected at 0, 30 and 75 minutes after feeding E4b/water with simultaneous oral glucose load (2.5 g/kg body weight). Chronic effect of E4b was evaluated by measuring glucose, fructosamine, triglyceride, total cholesterol and HDL-cholesterol in the fasting plasma at the beginning and at the end of the study period. The results of the acute test showed that E4b has significant hypoglycemic effects (sum of increments over basal value, MfSEM; Control vs treated: 24.82f2.99 vs 12.45f0.55 mmohl; p<O.OOl). Also on the 28th day E4b showed highly significant (p<O.OOOl) hypoglycemic effects. As compared to Control the treated group showed a significantly lower values of plasma fructosamine(l99fl6 vs fl54&9 pmol/l; p<O.O2), TG (84+6 vs 63f4; mg/dl; pcO.Ol), total cholesterol (64f3 vs 54f2; mg/dl; pt0.03) and LDL-cholesterol(20f2 vs lOf0.8; mg/dl; p<O.OOl). No significant change was observed in the HDL-cholesterol level. The data show that E4b decreases both hyper glycemia and atherogenic lipids in the plasma of type 2 diabetic model rats. The fraction needs to be further studied both chemically and biologically.

Pterospermum acerifolium is used as an antidiabetic

P956 Comparative Studies of Impaired Glucose Tolerance in Dogs ‘D-e&xl with S-Nitrosoglutathione and S-Nitroso-N-Acetylpenicill&ne DONOVAN MCGROWDER, Dalip Ragoobirsingh, Tara Dasgupta. Basic
Medical Sciences and *Chemistry, University of the West Indies, Kingston, Jamaica

S-Nitrosoglutathione (GSNO) has been used to prevent platelet activation in patients with severe preeclampsia and S-Nitroso-N-Acetpenicillamine (SNAP) as a potent vasodilator. The present study investigates the pharmacological activity of the nitric oxide (NO) donors, GSNO and SNAP on the plasma glucose and insulin levels in dogs. Plasma glucose levels were measured by the glucose oxidase method, while the insulin level was measured by radioimmunoassay. Captopril was used as the control in these experiments and statistical comparison was done by the student’s t-test. The 2 h postprandial plasma glucose concentration in dogs treated with 10 mg/kg of SNAP was 7.89 f 0.3 mmol/l compared with 6.11 + 0.20 mmohl in dogs treated with 10 mg/kg of GSNO (P < 0.05). The 2 h postprandial plasma glucose concentration in dogs treated with 20 mgkg of SNAP was 9.17 5 0.33 mmol/l compared with 6.06 * 0.20 mmol/l in dogs treated with 20 mg/kg of GSNO (P < 0.05). The decreased glycaemic control was accompanied by a significant decrease in plasma insulin concentration (P < 0.05). The results showed that SNAP at dosages used in this study elicited a hyperglycaemic effect compared with minimal changes in dogs treated with GSNO. These results provide novel information which is of importance in the understanding of the release of NO from GSNO and SNAP and its possible role in the development of type I diabetes.

P955 The Improvement of Insulin Resistance by Using Growth Hormone Reduces the Risks of Atherosclerosis in Obese Diabetic Patients JAE-HYUN NAM, Kap-Bum Huh, Hyun-Chul Lee, Young-Duk Song, Kyung-Rae Kim, Seung-Kil Lim. Inrernal Medicine, Yonsei University
College of Medicine, Seoul, Korea, Republic of

Track 4. Basic Research

Insulin resistance in visceral obesity constitutes a risk factor for the development of atherosclerosis. Insulin resistance in obese NIDDM can be improved by a decrease in visceral fat and an increase in skeletal muscle which may influence insulin sensitivity. Growth hormone (GH) therapy accelerates a lipolysis and promotes protein conservation. We evaluated the effects of GH therapy with diet restriction on lipolysis and anabolism, which can change body composition, insulin resistance and atherosclerotic risk factors in obese NIDDM. Twelve obese NIDDM patients(31-56yrs) were treated with recombinant human GH (GH; 1 unit/d, 5/wk) and with diet restriction (25kcahkg ideal body weight/day) for 12 weeks. They underwent anthropometric measurement, bioelectrical impedence for total body fat and lean body mass, as well as computed tomography for visceral and subcutaneous fat at the umbilicus level and the muscle area at the mid-thigh level. All subjects underwent and GH response to hypoglycemia. The insulin sinsitivity index (ISI) was measured insulin tolerance tests(ITT). The visceral fat area(VFA)/thigh muscle area(TMA) ratio was decreased more in the GH-treated group than in the control

P957 Functional Analysis of Mutations in PDXl Found in Patients with l)pe II Diibetes SUSAN C. CAMPBELL ‘, Louisa Shepherd’, Leif Groop2, Andrew T. Hattersley 3, Wendy M. Macfarlane ’ ’ School of Biochemistry &
Genetics, University of Newcastle Upon Tyne. United Kingdom: 2 Department of Endocrinology, Lund University, Malmo, Sweden; 3 Department of Diabetes and Vascular Medicine, School of Postgraduate Medicine, Exeter United Kingdom

Homeodomain transcription factor PDXl (pancreatic/duodenal homeobox 1) plays a critical role in normal pancreas development. In adult islets it plays an equally critical role in the glucose regulation of insulin mRNA production. Here we utilise a human ,!-cell line lacking PDXl to examine the effect of three mutations, G212R. P239Q and P242L, identified in