Code No: R522301

1

II B.Tech. I I Semester (R05) Supplementary Examinations, April/May 2009 BIO PROCESS ENGINEERING-I (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks

1. Discuss in detail about Chronological development of the fermentation industry. [4+12] 2. (a) What is aseptic operation and containment? (b) Describe a typical aseptic, aerobic fermentation process. (c) What is sparger? Describe different spargers used in fermentors. 3. Explain the factors to be considered for developing medium for animal cell culture. [16] 4. What is meant by filter sterilization? Explain its application in media sterilization. [4+12] 5. Discuss the following in detail (a) Stoichiometricallly limiting compounds (b) Growth rate limiting medium. 6. Differentiate the heterotrophic and autotrophic metabolism emphasis on energetics. [16] 7. (a) Explain the procedure involved in the determination of cell number density and cell mass concentration. (b) Give a short note on simple unstructured kinetic models for microbial growth. [8+8] 8. Explain the concept of product formation in bioprocess engineering with appropriate examples. [16] [8+8] [4] [4] [2+6]

Code No: R522301

2

II B.Tech. II Semester (R05) Supplementary Examinations, April/May 2009 BIO PROCESS ENGINEERING-I (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks

1. Mention about the Regulatory constraints of bioprocesses. 2. (a) What is aseptic operation and containment? (b) Describe a typical aseptic, aerobic fermentation process. (c) What is sparger? Describe different spargers used in fermentors. 3. (a) Explain the use of water as an important constituent for fermentation. (b) Describe the use of buffers for media preparation in fermentation. 4. (a) Explain Richard’s rapid method for the design of sterilisation cycles. (b) Describe briefly the scale up of batch sterilisation processes. 5. Explain in detail the stoichiometry involved in the cell growth and product formation. 6. Enumerate the major difference in photosynthesis between microbes and plants.

[6+10] [4] [4] [2+6] [8+8] [8+8] [16] [16]

7. (a) Differentiate between unbalanced growth and balanced growth (b) Explain the Monod model along with the equation. [8+8] 8. Give brief notes on structured models for growth and product formation with relevant examples. [16]

Code No: R522301

3

II B.Tech. II Semester (R05) Supplementary Examinations, April/May 2009 BIO PROCESS ENGINEERING-I (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks

1. Mention about the commercial applications of enzymes. 2. (a) What is aseptic operation and containment? (b) Describe a typical aseptic, aerobic fermentation process. (c) What is sparger? Describe different spargers used in fermentors. 3. Explain the factors to be considered for developing medium for animal cell culture.

[16] [4] [4] [2+6] [16]

4. Explain the kinetics of medium sterilisation and obtain a mathematical expression for specific death rate. [16] 5. Explain in detail the stoichiometry involved in the cell growth and product formation. 6. (a) Explain in detail the heat and energy in metabolic reactions. (b) Give a brief note on Crabtree effect. 7. Define the following: (a) Maximum growth rate (b) Yield on substrate (c) Mass doubling time (d) Specific growth rate [4+4+4+4] 8. Explain the concept of product formation in bioprocess engineering with appropriate examples. [16] [8+8] [16]

Code No: R522301

4

II B.Tech. II Semester (R05) Supplementary Examinations, April/May 2009 BIO PROCESS ENGINEERING-I (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks

1. Mention about the Regulatory constraints of bioprocesses.

[6+10]

2. What is meant by solid state fermentation? Explain the industrial application of solid state fermentation indicating the microorganisms, substrates and products. [6+10] 3. Explain the factors to be considered for developing medium for animal cell culture. [16] 4. (a) What are the consequences if a foreign microorganism invade a fermentation process. (b) Explain the methods to avoid this contamination. 5. Explain in detail the stoichiometry involved in the cell growth and product formation. [8+8] [16]

6. What are the major steps in aerobic metabolism of hydrocarbons? What are the end products? [16] 7. (a) Explain the procedure involved in the determination of cell number density and cell mass concentration. (b) Give a short note on simple unstructured kinetic models for microbial growth. [8+8] 8. Enumerate difference between growth and non-growth associated product kinetics. [16]