Funding Application for Joint Applied Research Projects
This document uses Times New Roman font, 12 point, and 1.5 line spacing and 2 cm margins. Any modification of these parameters (excepting the figures and their captions), as well as exceeding the maximum number of pages set for each section can lead to the automatic disqualification of the application.
1. Importance and Relevance of the Technical and/or Scientific Content Identified problem: The role of nutrition for human health is currently one of the priorities of policies and strategies to ensure the quality of life. The idea of food-health relationship, summarized by „we are what we eat”, is not new. Hippocrates postulated that „food must be our cure and the remedies be our food”. New in approaching the concept of healthy eating is the interest for the relation food quality – group of consumers. In addition, in case of foods of animal origin there is a concern for the impact of animal nutrition on food safety and food quality and thus it has been created the dough for the development of the concept Fork to Farm "which is the Activity 2.2., Theme 2 of FP7 KBBE programme”. In such a world in which the interest in healthy eating with more natural products is increasing, the presence and the role of hen egg and chicken meat in the daily basket is a challenge full of opportunities but which requires some changes in the production field of meat poultry and eggs. There was a significant concern to amend certain nutritional qualities in order to respond to the requests of those consumers that take into account the human medical advices or that already have a condition that imposes certain restrictions on cooking. Among the nutrients in eggs and meat, important for human health, there are included the polyunsaturated fatty acids. From these, omega-3 fatty acids are essential for normal growth and development and they can play an important role in the prevention and treatment of cardiovascular diseases, arterial hypertension, diabetes, arthritis and other inflammatory and autoimmune disorders, cancer, etc. Currently, there are many products on the market which are enriched in omega-3 fatty acids. The nutritional path chosen is that of enrichment of the feed diet of laying hens or chickens in omega-3 polyunsaturated fatty acids. To achieve this, there are included in poultry diets, especially, seeds and oils from oleaginous plants. There are situations when the food diets are optimized by including fish oil (tuna, herring, etc.). In both cases, altogether with the addition of feed raw materials rich in fatty acids with double bonds, it must be ensured the necessary amount of antioxidant substances which can eliminate the unpleasant effects of autooxidation of the lipids in feed and in consequence of the lipids in eggs and meat. Antioxidant means "against oxidation." Antioxidants work to protect lipids from peroxidation by radicals. Antioxidants are effective because they are willing to give up their own electrons to free radicals. When a free radical gains the electron from an antioxidant it no longer needs to attack the cell and the chain reaction of oxidation is broken. After donating an electron an antioxidant becomes a free radical by definition. Antioxidants in this state are not harmful because they have the ability to accommodate the change in electrons without becoming reactive. The human body has an elaborate antioxidant defense system. Antioxidants are manufactured within the body and can also be extracted from the food humans eat such as fruits, vegetables, seeds, nuts, meats, eggs 2
and oil. The most used antioxidants in feeds for poultry are: ethoxyquin (1,2-dihydro-6-ethoxy2,2,4-trimethylquinoline), BHA (butylate of hydroxyanisol) and BHT (butylate of
hydroxytoluene), vitamin E. Using these synthetic antioxidants presents also disadvantages for the health of animals and of consumers of chicken meat, chicken meat products and eggs. For instance, BHT, the most used additive from the poultry feeds, prevents antibodies response when poultry is exposed to the action of certain viruses, as it is the virus of Newcastle disease. This effect of BHT in doses of 100-150 ppm in feeds suggest a possible explanation for the losses occurred sometimes at vaccinations performed with live vaccines. Antioxidant effect of vitamin E becomes significant only at high rates of inclusion in feed (200-300 mg/kg), this source being very expensive, which raises the cost price of feed. Supplementary, synthetic antioxidants increase the incidence of cancer, alergies and degenerative diseases of consumers. Solution: At present, when man returns his face to nature, an alternative for synthetic antioxidants is constituted by the polyphenolic extracts from plants. Polyphenols from plants are considered non-nutritive substances, the interest for these compounds being due to the possible beneficial effects on human and animals health. For example, quercitine (a flavonoid) and ellagic acid presented anticancer activity in studies conducted on experimental animals, epigalocatechin gallate inhibits proliferation of tissues of cancerous tumors on the rats skin. The purpose of the project is to value local by-products of vegetal nature, as result of local food industry and food supplements industry, instead of the expensive synthetic compounds and harmful to health consumers of egg and meat poultry, for prevention of polyunsaturated acids peroxidation in the process of obtaining certain healthy food (chicken eggs and breast), enriched in omega-3 polyunsaturated fatty acids and consequently, with antioxidant effect on meat proteins. 1.1. The concept and objectives of the project
1.1.1. Project concept Phyto-additives used in animals feed are plants or products derived from plants which are embedded in animal diets with the purpose of improving animals productivity, or to increase the quality of foods obtained from these animals. There is a very large variety of vegetal compounds from both the point of view of their origin and technological processes (drying, extraction, etc), through which they pass before being used in animals feed. The reason for which, in last decade, the feed phyto-addtitives domain developed is banning at E.U. level, the use of antibiotics in animals feed, as a result of identifying the risk of occurence of bacteria resistance to the action of antibiotics increasingly used in animals feed. The use of phyto-additives with antioxidant role in the case of recipes rich in omega-3 fatty
acids in poultry is relatively new, because there are still studied both their mode of action and the aspects related to the application (local sources, by-products). Adding these vegetal materials in poultry diets raises both from the practical and technological point of view, and from the legal one, three major problems: “what for?”, “in what form?”, “how much?”. Vegetal materials targeted for their antioxidant potential will not be used with curative purpose, but with the goal to ensure and improve maintaining the quality of feed enriched in omega-3 fatty acids, and food (eggs, chicken meat) obtained from poultry fed the mentioned type of feed. Starting from the expression “Feed safety is part of food safety”, the producers, distributors and merchants on the feed market share the safety responsibility of food of animal origin with the players on the food market. Actually, the legislation from the domain of feed production tends to become part of the legislation of producing and marketing food. In this context, but also because of the potential economic benefits, project concept is related to using vegetal by-products from food industry as sources of antioxidants to ensure stability in time to the feed enriched in omega-3 fatty acids for poultry. The major problem raised by the use of vegetal by-products is related to the maintenance of antioxidant properties of the plants of origin. For this purpose, within the project, from the broad range of plants with antioxidant properties (rosemary, oregano, marjoram, seabuckthorn, sage, thyme, grapes, tomatoes, apples, carrots, lucerne, etc.) will be evaluated the compounds with antioxidant action which are found in by-products. Plant antioxidant activity is given by certain specific active compounds, which will be evaluated for each vegetal material under consideration. In the first phase of this project, will be evaluated the by-products resulted from technological processes (in SC Hofigal SA) for obtaining natural food supplements (for example, lucerne leaves contain carotenoids (lutein, zeaxhanthin, astaxhanthin); apple fruits contain flavonoids (rutoside, sinensetin, 3'-hydroxy-5,6,7,4'-tetramethoxyflavone), caffeic acid derivatives (5-caffeoyl quinic acid, rosmarinic acid, chlorogenic acid), catechin, epicatechin, ascorbic acid; grapes contain flavonoids (kaempferol-3-O-gluco-4p" sides, quercetin-3-O-glucosides), procyanidolic oligomers (proanthocyanidins), catechin, epicatechin, epicatechin gallate, resveratrol). Following selection of vegetal materials by tracking criteria related to the local specific and content in substances with antioxidant potential, these must be evaluated from the point of view of antioxidant capacity, pursuing a series of parameters – primary products of lipidic peroxidation: extractions of total phenolics; total phenolic content; flavonoid content; quenching effects of alcoholic extracts on reactive oxygen species, the ability of polyphenols to reduce ion Fe3 +, the ability of polyphenols to chelate transitional metal ions, the determination of the protective effect of substances with antioxidant potential of PUFA in feed: determination of compounds mayors
(peroxides and conjugates diene) and secondary lipid peroxidation in animal feed (TBARS). After selection of optimal vegetal materials, using above mentioned criteria, will be set, according to a calculation algorithm, the inclusion rate in hens and broilers feed. The resulted feed will be subject to the same type of determinations, so that its complete characterization will be obtained. In addition, determinations concerning the maintenance of feed quality for a month period will be established. To study the effects of inclusion vegetal materials with antioxidant capacity in feeds enriched in omega-3 for poultry, tests on animals are necessary to evaluate antioxidant status of poultry and oxidative processes at the level of lipids and proteins: extraction of total lipids; evaluation of conjugated dienes; evaluation of peroxide value; evaluation of thiobarbituric acid reactive substances (TBARS); evaluation of myoglobin concentration and relative concentration of metmyoglobin; evaluation of soluble proteins; evaluation of oxidized proteins. Due to the complexity of the characterization process from the point of view of antioxidant capacity of vegetal materials, feeds and animal products, within the project will be followed the development of a set of chemical methods to determine antioxidant capacity from samples of animal origin (serum, liver, brain, kidney) simple, sensible, selective and well-suited for the types of analyzes. In addition, the bioproductive parameters will be followed and based on chemical and physical analyses the nutritional quality of hen eggs and broiler meat obtained during the experiments will be evaluated. Microbiology and mycotoxicology methods will be used for the quality control of feed, eggs and meat. Heavy metals and microbiological load of manure birds it will be determined. After collating the obtained results a technical documentation will be developed, feed recipes resulted after unfolding the project will be subject to 2 patent applications. 1.1.2. Project objectives Technical and scientific objectives Nutritional solutions for poultry, innovative and integrative by inclusion of vegetal
by-products resulted from food industry Ensuring of natural alternatives, of vegetal nature, to the synthetic compounds,
expensive, which are used in poultry feed to prevent polyunsaturated acids oxidation Obtaining of functional foods, eggs and chicken meat enriched in omega-3 fatty acids, by procedures and technologies “friendly” to the animal, consumers and environment safety, ERA (European Reserch Area) priority Creating of a partnership between research facilities and an economic entity, in
order to solve certain problems with social and economic implications, and creating the 5
Dissemination and exploiting of the obtained results. The project will be developed in 5 phases: 1. “in vivo”. a series of vertical activities will be developed: (i) the coherent 6
. we propose that by the interdisciplinary applied research activities to fathom the knowledge of the subject research and to find a solving practical solution. University of Agronomical Sciences and Veterinary Medicine Bucharest –Faculty of Veterinary Medicine Veterinary Medicine Veterinary Medicine (P2). S . During these 5 phases of the project. 4.mechanisms to implement innovative solutions resulted from the project Increase of competitiveness and international visibility of the research team from
the project consortium Ensuring the production of sustainable food. working techniques and economic analyzes of evaluation of effects of using some by-products of vegetal origin as antioxidants in the feed of laying hens and broiler chickens. profitable for human health. “in vivo”. To achieve these objectives a consortium has been created consisting of: the project’s coordinator (CO) .Faculty of Pharmacy (P3). University of Bucharest – Faculty of Chemistry (P4). 5.A (P1). from the perspective of the costs of ensuring public health Economic objectives Using food industry by-products with local specific as sources of antioxidants for
poultry feed. Multidisciplinary studies regarding methodologies. over the 5 stages. by increasing the impact of the
direct implications on health system by reducing direct and indirect costs associated to people with predisposition for cardiovascular diseases The project objectives matches with the programme PN-II-PT-PCCA-2011-3 objetives for domaine 5. Experimenting. Hofigal Export Import S.National Research Development Institute for Animal Biology and Nutrition (IBNA). Physical and chemical characterization of by-products (selected at the end of phase 1 to be physically and chemically characterized from the perspective of the antioxidative potential and facility of conditioning for embedding in feed) of vegetal origin and valuing their efficiency as feed additives with antioxidant role in laying hens and broilers feed. Promotion of a healthy lifestyle with role in occurrence of coronary disorders. As a result. enriched in ω3FA. to determine the effects of by-products (selected at the end of phase 2) used as antioxidant additives in broilers feed. based on scientific research to obtain certain foods. to determine the effects of by-products (selected at the end of phase 2) used as antioxidant feed additives in laying hens feed. University of Medicine and Pharmacy Bucharest. 3. as an alternative to expensive synthetic products constitutes a way to value vegetal materials without economic potential. considered waste products. 2. Experimenting.
The possibility of providing animal products with high nutritive value. animal breeders are stimulated to invest in the industry producing functional foods. This way. The vegetal materials used as phyto-additives present the disadvantage of a variable chemical composition depending on the collection area. and economic barriers It is known for a long time that there is a relationship between the food we eat and our health. and with beneficial effect on health constitutes a great advantage for both eggs and chicken meat producers. and from identification of mechanisms by which food modulate metabolism and health. with low costs. and for their consumers. and the levels of vegetal materials considered as natural sources of antioxidants. The concept of by-product is related to using a vegetal material from which were extracted a series of compounds considered important in food industry. is surpassed the technical barrier related to the status of “waste” of food industry by-products. based on which to be possible to decide using them or not. The current concept of functional food resulted from gradual acknowledgment of the fact that healthy diets result from consumption of food with rich content in nutrients. in a great measure. The scientific barrier surpassed by the project consists of obtaining certain functional foods (eggs and chicken meat) cheap and safe. by the idea of the project. Technical. Through the idea of using food by-products is overcome. The growth of the functional foods sector not only represents significant benefits to the health sector. Manufacturers and their search for added-value. (ii) the verification and validation of the theoretical concept through design and results of experimental studies. By characterizing vegetal materials and highlighting their antioxidant potential. scientific. but also offers opportunities for processing and manufacturing companies. climate etc.1. The barrier overcome by solutions proposed in the project is substitution of synthetic antioxidants with by-products of local food industry in order to obtain certain functional foods (eggs and chicken meat) enriched in omega-3. the
. and consumers are encouraged to adopt a healthy lifestyle. Valuing these vegetal materials leads to economic advantages for producers of food..3. their certification being complicated. by nutritional means. The scientific barrier overcome through the project refers to the elaboration of a protocol of characterizing by-products from the point of view of antioxidant substances and capacity.enunciation of the theoretical concept. 1. using optimized feed rations with impact on eggs and carcasses quality. higher margin products provided key impetus for the growth of functional products. feed or animal products. (iii) the theoretical applied approach concept through implementation and results valuing.
these conditions are met by the plants harvested from the spontaneous flora. found also at international level in the requirements of FP7 (“Farm to Fork”) program.scientific barrier related to their characterization. climate. leaves.1. The disadvantage presented by this category is related to the variability of the biologically active plant compounds depending on the soil. the content of active substances from products may vary a lot. antioxidant capacity. their chemical composition being relatively constant (cultured raw materials. and tree bark). Within vegetal materials.4. contributes to the development of knowledge in a domain without broad bibliographical support. 1. are valued vegetal materials considered waste. with deliverables . according to the used part of plant (for example. with deliverables . Promoting consumer wellbeing amid health ensuring led to the development of certain strategies of obtaining and promoting safer. it is eliminated the variability of biological compounds. enriched in ω3FA. on eggs and meat enriched in ω3FA. harvesting season and geographical origin. from the point of view of: compounds with antioxidant action. grown in controlled conditions. Originality and innovative nature Perspective concerns of food industry have in view obtaining foods by “friendly” procedures and technologies. Using certain local vegetal materials as antioxidants sources in feeds enriched in omega-3 for poultry. At present. for laying hens and broilers. On one side. economic interest leads to the identification of some natural antioxidants sources with beneficial effect on animals health and with low cost. polution etc. roots. By using certain cultured plants. As results will be expected : the development of a nutritional strategy to optimize feed recipes. Another solution identified and followed in the present project is using by-products from food industry as natural antioxidants sources. under the conditions of a world which is changing. Characterization of vegetal by-products. and nutritive potential. on European market. the decreases of unwanted effects of polyunsaturated fatty acids oxidation from hens and broilers rations. and from the economic point of view. seeds.2 licence certifications for byproducts of vegetal origin used as antioxidants in poultry feed wherein the beneficiary is P1 of the
. In this category are nutritional methods of influencing the quality of food products of animal origin. feeds obtained by their inclusion and of products resultated after tests on animals. healthier and high quality food. strategic segment of economy. is sustained the increasing competitiveness of Romanian food products industry.2 patent applications applied at OSIM (a feed formula for hens and a feed formula for broilers) wherein the beneficiary is the project’s coordinator. obtained in the same conditions).
as additives with antioxidant activity.logistic support for project’s achievement. safe with deliverables – brochures for information and awareness of the consumer wherein the beneficiaries are manufacturers of food industry and non-governmental consumer organizations a framework methodology for evaluation of the vegetal by-products potential.the development of a competence center.incomplete answers.C. scientific or nonscientific popularization articles (revues as – Fermierul.the rise of the degree of visibility of our approach. HOFIGAL EXPORT IMPORT S. producing local functional food (egg. products added fodders. . 1. with deliverables workshops. AGRALIM.
.S.the quality of the work team achieved in previous research projects. State of the art 9
. added to the diets of poultry.special opportunities for the formation of . .USAMV Bucharest and seminars at the Faculty of Pharmacy from UMF Bucharest SWOT Analysis: Viability and risks of the project. participation at national exhibitions (INDAGRA. Recolta). roundtable communications.the approach of new themes in order to develop them in European programs. with deliverables – teaching support courses.the problem of antioxidant capacity evaluation from a multitude of samples. Strengths . Threats .the impossibility to certificate the byresearch in mainstream journals. research institutes by publishing scientific . wherein the beneficiaries are students of Scientific Society at the Faculty of Veterinary Medicine . chicken meat) relatively cheap.the novelty of the team might come that some of the hypothesis will not confirm. . P2.A . AGRARIA) addressed to the business field wherein the beneficiaries are –
manufacturers from feed and poultry industry and the business environment.the need to re-formulate the experimental Weaknesses . partners in consortium with deliverables min.2. the increase of the visibility of competence and expertise of the research teams.project . at national and international level wherein the beneficiaries are young researchers of: CO. P3 and P4 a stimulation of partnerships between research units and private businesses. young PhD students. Lumea Satului.
. 4 articles for journals with impact factor and min 6 communication. Opportunities . a partnership between research facilities and an economic entity with deliverables – a business plan wherein the beneficiary is P1.
but it may promote better health and play an important role in disease prevention by reducing the risk of certain chronic diseases. experimental and clinical studies have shown that certain types of food and specific food components can affect a variety of body functions and provide health benefits. flax oil. At present. margarine and salads dressings. they would be open to functional food components if added to or present in foods they already eat and like. Eggs can make a valuable contribution to a healthy. and soy). More than half the calories found in eggs come from the fat in the yolk. there are many products on the market which are enriched in n-3 fatty acids. diet not only helps to achieve optimal development and health. it is important to educate the consumer on the importance of a balanced. eggs (including in poultry feed of fish flour. bakery products (flaxseed flour and capsulated fish oils (Andersen. According to an Euromitor survey.6 billion dolars). The question of cholesterol in eggs – a previous concern that resulted in lower consumption – is an important subject.7 billion dolars.5 billion dollars while the European market is less developed with an estimated market of 7. generally recognised as safe additives. were used by nutritionists to alter the diet of laying hens and reduce cholesterol content of eggs. Among the different products delivering essential nutrients to the body. infants formulas. Adding oils rich in polyunsaturated fatty acids (PUFA) to poultry diet has the effect of increasing the occurence of the lipidic peroxidation process. the “big four” European markets being UK (2. Synthetic antioxidants
. flax seeds. However epidemiological. an egg has arguably a special place. the primary role of the diet is to provide adequate quantities of nutrients to meet metabolic requirements and maintain optimal health. farmed fish.5 billion dolars. milk.4 billion dolars). and Italy (1. Diet plays an important role in maintaining health.2 billion dolars). Natural products. mayonnaises. Among these can be mentioned: oils rich in ALA (canola. or DHA from algae led to the enrichment of eggs in omega-3 fatty acids). used in producing bakery products.According to the traditional concept of nutrition. Health professionals can help consumers develop a positive attitude toward functional foods. 1995). balanced diet in that they provide high quality protein and a number of vitamins and minerals. while unmotivated consumers were skeptical of information on diet and health. Therefore. the lipidic peroxidation process attracts the necessity of using synthetic antioxidants in feeds. People on a low-cholesterol diet may need to reduce egg consumption. perilla. including manufacture of bread). A survey conducted for the International Food Information Council found that. meat and products from poultry. then US is the second one market with around 10. However. Germany (2.4 billion dolars). In its turn. healthful diet rather than on single nutrients or functional components. Japan is the world's largest market at 11. France (1.
stabilizers. Plants improve use of feeds and have favorable effects on poultry (laying hens and broilers).vitamins. and on zootechnical performances of animals. namely: influence the taste and flavor of feed. 2008d).preservatives. 2008b). zootechnical additives . 2008a. mineral components. antioxidants added in feed are of synthetic nature. as well as other compounds from feeds destined to poultry. 1. in Romania the most frequently used being vitamin E.2. To cover the necessary vitamin E for laying hens and broilers. 2007. the interest in these compounds being due to the possible beneficial effects on human and animals health. antioxidants.protect fatty acids.pro. Studies about the effect of Selenium on stability of feeds for pig and chicken. Tagetes Patulus) on the stability of feeds enriched in omega-3 fatty acids destined to poultry (Criste et al. as it can happen in case of using vitamin E as antioxidant in feeds with fatty substances. stimulte animal organism and have a positive effect on the color of egg yolk and fowls. Panaite et al. when administered in excess emerge also its harmful effects. Panaite et al. 2008c. Ropota et al. emulsifiers. In Romania. aminoacids. sensorial additives . it was studied the effect of plants (Marigold (Calendula Officinalis). State of the art at national level At present. aromatic substances. in Romania. A team of researchers from Romania (Panaite et al. Plants polyphenols are considered non-nutritive substances. Because animal organism can store vitamin E. Nettle (Urtica Dioica).and prebiotics as stabilizers of intestinal flora. must be added in their feed a commercial vitamin premix which has to contain approximately 30 ppm. 2008. but have also negative influence on their health and on the health of consumers of chicken meat and eggs. contain numerous bioactive components. fatty acids. and coccidiostatics and hystomonostats. Use of plants in poultry feed is economic and natural. five categories of food additives are favored: technological additives . nutritional additives . 2008a) studied the antioxidant effect of vitamin E using different inclusion rates in hen feed and found that the feed supplemented with 250 ppm vitamin E increased the shelf life of the feeds. but in the case vitamin E should fulfill the role of antioxidant must be added a quantity of approximately 200-300 ppm. food enzymes. An alternative to the synthetic antioxidants could be polyphenolic extracts from plants.colorants. published an article regarding the adjustment of quality control of feed enriched in omega-3 and vitamin E. additives used at ensiling. were conducted under the direction of teams of scientists from Romania (Panaite et al. Antioxidant capacity of feed enriched in oregano and cranberries was studied in view of
. In compliance with the amendment of European Parliament with respect to the ordinance 1831/2003. Panaite et al.1.
2009). 2007..0% savory. following undertaken studies (Criste et al. Echinaceae herba. 2000. Some plant pigments (antocyanine and antocyanidine) may chelate metals and give away H towards the oxygen radicals. rosemary. 2002. Plantago major.. The plant extract are generally used for their aromatic properties. Phoeniculus sp. green tea.1. to the flavonoids (quercetin.. 2003. grape seeds and oregano.their use in experiments on pig (Papuc et al. thus being extremely efficient antioxidants. Chelidonium majus. Smet et al. particularly when 1. Sasse et al. The results have shown that the supplemental plants as natural antioxidants used during the laying period. carvacrol. Tang et al. Maa et al. At the national level. in pigs. phenolic acids. Most natural antioxidants are fat-soluble phenols. The savory and rosemary also had a positive effect on the oxidative stability of the stored eggs. tymol and menthol). Taraxacum officinale. 2009).. Brenes et al.. caffeic and rosmarinic) to the phenolic diterpenes (carnosol. Radwan et al. In another study. The tea and the extracts from grape seeds and peel contain catechins. Alia et al. .donor activity. determined the antioxidant effect of savory. proantocyanidines and resveratrol.5% Curcuma longa were used. or 0. Malva silvestris. rosemary. rosmanol and rosmadial). Crataegus monogyna..2.2. and in the muscle tissue post-mortem (in chicken chicks. catechin. in chicken chicks. may improve layer performance. thus slowing oxidizing by two mechanisms. Ahn et al. This antioxidant activity is due most time to the phenolic acids (gallic. 2008c) were implemented in poultry industry feed recipes designed to obtain eggs enriched in omega-3 fatty acids using feed plants and oils rich in polyunsaturated fatty acids. Rojas and Brewer 2007. Melissae folium) from the local market were analyzed for total antioxidant and scavenging capacities for including in poultry feed (Stef et al. in chicken chicks. 2008). all of them contributing to the antioxidant activity. carnosic acid. and kaempferol) and to the volatile oils (eugenol. Artemisia absinthium. epictechins. antioxidative status and immune
. The literature has several studies showing the antioxidant capacity of some plant products used in poultry feeding. sage.. 2008b. 2008a. Epilobium montanum.. naringenin. State of the art at international level A large number of herbs and aromatic plants contain compounds that can be extracted and used in foods to prevent oxidation (Lee and Shibamoto 2002. 2010). 2003) and in vivo in plasma (in rats. such as phenols from the rosemary. but also often have an intense H. protocatechic. a total of eleven medicinal herbs (Rhamnus frangula. Many of these extracts and their purified components have in vitro antioxidant activity (Blazovics et al. 2008. Hasty et al. 2005 studied the effect of Ligustrum lucidum and Schisandra chinensis on egg production. Criste et al. oregano. oregano and şi Curcuma longa on the productive and reproductive performance of local hen breeds and determined the oxidative stability of the yolk from eggs stored at room temperature (16oC ± 2). 2008).
The effect of adding green tea and its aqueous extract as natural antioxidants to local laying hen diets on productive. 2011 worked on broilers too (800 day-old male Ross-308 chicks) monitoring the effects of the supplementary rosemary. oxidative stability.. The oxidative status and lipid oxidation of broiler plasma was not affected by the dietary natural antioxidants. liver. α-TOC status. and vitamin E concentration of n3 fatty acid-enriched eggs. especially during storage. Smet et al. n − 3 PUFA (flax seed and marine algae) and rosemary may be used to hens fed diet without affecting the sensory properties of eggs. but the activity of the antioxidant enzymes changed slightly. The study also used a diet with synthetic antioxidants with or without alpha-tocopherol acetate (200 mg/kg feed). (2010) investigate the effect of dietary antioxidants and storage on fatty acid profile. It was concluded that green tea powder inclusion in the diet for layers can reduce the cholesterol content and TBA value of the egg yolk. grapes and tomato seeds) on lipid an protein oxidation in the broiler meat.. 2006). grapes seeds.05). Hayat et al. The extract was added to the drinking water during the final 14 days before
. The natural tocopherols and the rosemary showed the best effects at an inclusion level of 200 mg·kg–1. 2011. reproductive performance and egg quality during storage and its content of cholesterol it was studied by Zeinab et al. volatile rosemary oil and alpha-tocopherol acetate (vitamin E) on serum parameters using a soybean-corn based diet. Polat et al. The results show that 1% Ligustrum lucidum and Schisandra chinensis has beneficial effects on egg production under heat stress (p<0. Ligustrum lucidum and Schisandra chinensis reduced significantly the heart. These studies demonstrate that the level and type of antioxidants and duration of egg storage significantly affected the fatty acid profile. green tea.. serum end yolk malondialdehyde. 2011 studied the effects of the watery mate (Ilex paraguariensis) extract given to broiler chicks.status of the layers under heat stress. tomatoes) on the antioxidant status of the plasma in chicken chicks fed on feeds rich in polyunsaturated fatty acids. and oxidative stability of chicken eggs. rosemary. The concluding was that Rosmarinus officinalis and its volatile oil increased serum SOD activity and affected positively the oxidizing mechanisms. The results suggested that vegetable lipids (palm butter. flax seed). Racanicci et al. (2010). green tea. in addition to improving the productive and reproductive performance of both hens and roosters. 2008 monitored the influence of the supplementary natural antioxidants (natural extracts of tocopherols. investigated the effect of the antioxidants from the linen oil and plant extrscts (rosemary. grape seed.. implying its potential effect on egg quality parameters. In onther work (Parpinello et al. it was report the first sensory characterization of eggs and products containing eggs obtained from hens diet based on grape and algae plus vitamin E and rosemary extract. The extracts were added as such or in combinations ion the diets with 4% linen seeds oil. Vossen et al.
oxygen radical absorbance capacity (ORAC) and total radical trapping antioxidant parameter (TRAP). It is estimated that half of the world’s fruit and vegetable crops are lost (Martinez et al. the result was significant after 3 days too. working on day-old male Arbor Acre chicks. which many times and mixtures.5:48. 2008. These results are in agreement with those of Jang et al. Yu. who used a mixture of blackberry leaves. The best result was noticed during the first day of storage. flavor. where it is a major causes of chemical spoilage (Colbert et al. Donga et al.) and B) some journals dedicated exclusively to this topic (Free Radical Biology and Medicine.5:3. 2005). that it is worth mentioning: A) some relevant books published in the field (Halliwell.0). There has been a tremendous increase in the research related to reactive oxygen species (ROSs). Antioxidants and Redox Signaling. The mixture increased the antioxidant potential of the broiler breast meat during refrigeration. Free Radical Research. 2011. So much work has been done in this area. ET-based assays measure the capacity of an
. Japanese honeysuckle and goldthread (48. This is why the determination of the active compounds allows the efficient use of this type of phytoadditives. to antioxidants and to oxidative stress and the number of papers dedicated to these subjects has been increasing exponentially in the last two decades. Oxidation can affect foods. when the oxidative rancidness was very high in all samples.C). 2007. texture and safety of foods. 1991) resulting in rancidity and/or deterioration of the nutritional quality. 2007. The evaluation of the biological effects is difficult if the composition of the tested substance is not stated or variable. Ho. with more than 50% reduction in TBARS. increased the antioxidant potential of the broiler breast meat with polysavone (natural alfalfa extract). Conventional methods for the determination of antioxidant capacity can be roughly classified into two types: assays based on hydrogen atom transfer (HAT) reactions and assays based on electron transfer (ET) (Huang. The phytogenic additives vary a lot in their in vivo effects on animals. The determination of the active compounds which are antioxidants is also important. colour. Defense mechanisms against the effects of excessive oxidation are provided by the action of various antioxidants and the need to measure antioxidant activity is well documented. Guteridge. 2008. The oxidative stability of the meat preparations made from the control and experimental groups was checked. The data on the way in which the marketed phytogenic additives work is rather vague. This variation is due to their different composition and to the biological effects of these feed additives. after refrigeration for up to seven days. 1995) due to postharvest deteriorative reactions. Shahidi. HAT-based assays include inhibition of induced low-density lipoprotein auto-oxidation. but it almost disappeared after 7 days.slaughter.
g. iron ions. 2007) including epoxides. butanones. Peroxide value (PV) Limitations involving this procedure are well recognized and include poor sensitivity and selectivity. Guteridge.2-diphenyl-1-picryl hydrazyl radical) method. The MDA is reacted with thiobarbituric acid (TBA) to form a pink pigment that is measured spectrophotometrically at 532-535 nm.. e.antioxidant in the reduction of an oxidant which changes color when reduced. Thiobarbituric acid reactive substances (TBARS) assay. This procedure measure the MDA formed as the split product of an endoperoxide of unsaturated fatty acids resulting from oxidation of a lipid substrate. Measurement of hexanal and related cend-products. with oxidation being initiated by the adition of copper ions. Diene conjugation resulting from lipid oxidation (Heinonen et al. o-diphenols contribute over 50% to the stability of virgin olive oil. The TBAS assay is now the most commonly used method to detect lipid oxidation. 1998) is now commonly used as an end-point for determining the antioxidant activity of a sample. pentanones. Accelerated stability tests. chemiluminescence
. ET-based assays include the total phenols assay by Folin-Ciocalteu reagent (FCR).) on plant biophenols. 1999.g. Decomposition of the primary products of lipid oxidation generates a complex mixture (Halliwell. The usual substrate for the determination of the conjugated dienes includes any substrate containing polyunsaturated fatty acids. None of the methods mentioned above has general applicability. oxidation of iodide by dissolved oxygen and variations in reactivity of different peroxides. ketones (e. End-point detection has been based on measurement of fluorescence inhibition. possible addition of iodine across unsaturated double bonds leading to low results. octanones). Methods have been developed for measuring the antioxidant activity as the ability to scavenge free radicals generated in aqueous and lipophilic phases. The TBARS procedure is widely used even though the reaction is not very specific and reaction conditions have a significant effect on the colour development. These accelerated tests are specific to the analysis of oxidation in food with results usually expressed as an induction time. The electron spin resonance (ESR) spin trapping assay could be used for antioxidants determination. There is intense interest in identifying natural antioxidants for use in foods and there has been considerable focus (Bonilla et al. Following oxidation the end-point is determined by measuring parameters such as peroxide value. Measurement of free radicals. assay using a Cu(II) complex as an oxidant (CUPRAC) and DPPH (2. hydrocarbons and saturated and unsaturated aldehydes such as hexanal. trolox equivalence antioxidant capacity (TEAC) ferric ion reducing antioxidant power (FRAP). AAPH or DDPH or the application of heat. In the following we shall shortly describe the main methods for measuring antioxidant activity related to lipid oxidation. Stability tests for edible oils commonly involve accelerated deterioration tests. conductivity or diene conjugation. Diene conjugation.
Quality and Safety of Feeds and Food for Europe (QSAFFE – FP 7) collaborative project No. whose inventors are Durand D. No. Date: 2008-03-26. which have shown carcinogenic characteristics. Flavonoids in fruits and vegetables : their impact on food quality. K. [Fr]. taurine. they have invented a compositions suitable for enhancing the antioxidant status of animals containing methionine.3. 1. Date: Aug.
. The information shows that the project is not in conflict with any of the ideas already presented in the literature. whose authors are K. Pub. have an application patent on a cost –eficient additive derived from a plant material originating from olive (Olea europaea L. vegetal byproducts.:US 2008/0187620 A1. As for patents.absorbance. Comparison between our product that we aim to develop and other products available worldwide There are lots of scientific papers regarding this subject. Yamka. Pub. M. when breeding and producing livestock. Carthagen IN (US) and R. but not so many patents on animal nutrition area. Vitamin C. Gladine C. Bronby Strand (DK). whose autors are B.7. The CLM methods are generally more sensitive than the conventional methods used for ROSs and for antioxidant determination. Nielsen. In principle.) effective as addition to feed. G. None of these patents or projects analyses Romanian local sources. etc. K. Date: Jan 28. March 2011 The patents and projects related to the idea of the project refer to the use of natural sources in animal feeding. Elgaard. No. Pub. Plants and their extracts and other natural alternatives to antimicrobials in feeds (REPLACE FOOD-CT-2004-506487). [Fr]. [Fr]. Bauchart D. Hvidovre (DK) and T. Medina B. [Fr].2. et al. ROSs form with the CL reagents excited species that generate CL radiations. Gruffat D. [Fr]. 2010. 265702. and stable light emission is luminol (Prior. prolonged. 2005). and vitamin E. Friesen.: US 2010/0022636 A1. The application patent „Plant-based natural antioxidant additive intended for animal nutrition” Pub. Topeka (US) . The most widely used CL reagent to trap oxidants and convert weak emissions into intense. Several national and international research projects approached topics in the domain of this project: New Phytogenic Product Line for Pig Production (PHYTOGENIC ADDITIVES QLK5CT-2002-70928). [Fr]. used as a substitute for traditional growth promoters and as a substitute for synthetic antioxidants. 2008. nutrition and human health (FLAVO). [Fr] have invented an antioxidant additive herbal extract comprising extracts of more thant 10 herbs used preferably in ruminat’s feed. Recoquillay F. Another application patent “Composition and methods for enhancing the antioxidant status of animals” Pub. Picaud T. in “Natural antioxidative additive for feed and products use for humans”.
Tamas et al
(2007). egg thickness.
chromatographic analysis. trace elements (atomic absorption spectrometry). CO’s team has contributions concerning the enrichment of laying hens diet in ω: 3-PUFA (Criste et al. Haugh unit. purity and quality of a herbal drug. The main goal of this studies were to evaluate the potential of a layer diet with
different levels of linseeds or linseed oil to produce ω: 3-PUFA-enriched (alpha-LNA and DHA) eggs and to monitor layer performance.2.. There were good results when were used 5% linseed in layer diets. ( 2008) highlights the high antioxidant activity of polyphenols from Sea buckthorn fruits (Hippophae rhamnoides).1. zearalenone in samples by ELISA . Microbiological investigations are represented by determination of total number of germs. total ash and ash insoluble in hydrochloric acid. Qualitative chemical analysis for confirming the presence of a chemical compound (which may be
. total number of coliforms. Alfatoxins B1. crude protein and crude fiber (Kjeldahl method).. total number of Escherichia coli culture. microscopic examination. deoxynivalenol.. Toxine T2. soluble-substances. swelling index. Papuc et al. Identity parameters: macroscopic examination. yolk color intensity and freshness egg.enzyme-linked immunoassay method. Popa et al. Aslo a common collective of authors (CO and P2) has published a paper on the effect of some mineral and phytogenic additives. (2011) have characterized some plants extracts with
antioxidant traits.( 2008) has a study regarding relation between dietary vitamin E and yolk cholesterol. Scientific/technical contribution: The contribution of the project research teams at state of the art is diverse. Macroscopic examination to determ the morphological and anatomic characteristics. ash (gravimetric method). The paper has common collective of authors from CO and P1. 152 / 2009) allow the determinations of the dry matter (gravimetric method). An adequate methodology must be used for to analyse a vegetal raw material and organic matter. Mycotoxicological investigations highlight the concentration of mycotoxins as Afla − aflatoxins. assay of active principles. qualitative chemical analysis. fatty acids (gas chromatography method).Pharmacognostic analysis” comprises qualitative and quantitative tests in order to verify or to establish the identity. . Physical and chemical methods (according to Regulation (EC) no. fumonisin. Gird et al. (2010) have contributions to determination of some plants active components. The physico-chemical analysis laboratory is accredited ISO 17025. ochratoxin A. has a paper regarding antioxidants of plant origin. (2009) and Tudor et a.. cholesterol (gas chromatography method). rich in polyphenols (Papuc at al. total number of fungus on poultry feed and feces and by determinations of Salmonella spp on eggs and feces.4. 2010). Criste et al. used in fodder premixes to obtain consume egg with new qualities of functional food. Quality parameters: loss on drying. Panaite et al. 2008). whites pH and yolk pH (electrometric method). 2009. Purity parameters: foreign matter.
M. Gon˜ i. Acta Biologica Szegediensis. (2008b.. Bravo.. evaluation of peroxide value (PV). Evaluation of antioxidant activity of plant polyphenols: spectrophotometric determination of total phenolic content (TPC) ( based on the reaction of FolinCiocalteu reagent). 47.. G. Nutriton Research 23. 2008. Centeno. Lugasi.. Viveros. F.1999. 3. using oleaginous seeds high in polyunsaturated fatty acids. At the national level. evaluation of oxidized proteins 1.not the main active principle). the annihilation of hypochlorite anion.5. Spectrophotometric methods. in poultry production. Superoxide anion scavenging activity. I. I. 2003. Sa´yago -Ayerdi. Merida. 2008c) feed formulations have been implemented in the poultry industry..The researchers from the coordinator’s team were involved in several projects investigating then use of plants in animal feeding. Preliminary results . M. 209-215. HPLC methods). new functional food on the Romanian market (CEEX 22/ 2005). K. Blazovics.. 2. 1–4. the annihilation of hydrogen peroxide. C. HPLC. 2003. 4. evaluation of soluble proteins. 66(2). Assay procedure for chemiluminescence emission kinetics and for quenching effects of alcoholic extracts on reactive oxygen species: the annihilation of hydroxyl radical. Extraction of phenolic compounds from red grape marc for use as food lipid antioxidants. Qualitative chromatography: TLC. L. A.2. A. Horcajo.. Bibliography: 1..
peroxyl radical scavenging activity. C. A. A. F.. evaluation of myoglobin concentration and relative
concentration of metmyoglobin. GC.. Reducing power of the natural polyphenols of sempervivum tectorum in vitro and in vivo. Effect of grape pomace concentrate and vitamin E on digestibility of
. Mayen. These were: (i) Development of Natural Alternatives to Anti-microbials for the Control of Pig Health and Promotion of Performance (FEED FOR PIG HEALTH – FOOD-CT-2004-506144). (iii) Interdisciplinary research on the use of Morus sp. Alia. for the production omega 3 fatty acid-rich consumer eggs. Swelling index expressed a high content of mucilage in an herbal matter (Titrimetric methods. Arija.. (ii) Optimization of the compound feed formulation in terms of vitamin E/selenium/fatty acids ratio to improve broiler carcass quality (AGRAL 132/ 2002) – project director Criste Rodica Diana . and Medina.. Brenes.. SauraCalixto.... S. Bonilla. M..project director Criste Rodica Diana. following the investigations by Criste et al. Food Chem. spectrophotometric determination of flavonoid content. L. the annihilation of singlet oxygen. (iii) Optimization of the feeding factors to produce consumer eggs enriched in omega 3 polyunsaturated fatty acid. Goya. 1251–1267. Effect of grape antioxidant dietary fiber on the total antioxidant capacity and the activity of liver antioxidant enzymes in rats. evaluation of thiobarbituric acid reactive substances (TBARS). J. Evaluation of lipid peroxidation in meat: evaluation of conjugated dienes. Szentmihalyi.
.. 11-21 7. Poult Sci. 17. J.. 11-21. Larick. Improvement of egg qality by using diets optimised in terms of polyunsaturated fatty acids (PUFA). Martinez. 4th ed.J.. Rachieru. D. E. Food Sci... Heinonen. Ropota. 50(17).B. R. A. 10. M. Lee. 1248-1250. J.. and Jabbar. Ciurescu.. Songa. 2010. 89 .. Determination of antioxidant potential of volatile extracts isolated from various herbs and spices.. D.. and Decker. 2002. Antioxidant Activity of Berry and Fruit Wines and Liquors.. Oxidative stability and lipid components of eggs from flax-fed hens: Effect of dietary antioxidants and storage.G. T. C... & Zhanga. T. 2002.. 5. H. D. Shibamoto.M.. M. 2005.. R.. C. 1998.. 195-200. H. and Jo. S. Poultry Science. Van Heuten. Hayat.. folium. Pasha.. 2008.L.. antioxidant status and immunity of laying hens during heat stress. “Free Radical in Biology and Medicine”. J. Comparative Chemical Study of Passiflorae incarnatae flores. Panaite. Gîrd et all. 46 (1). Z. 14.. Maa. Lee. pag. 52(3). I. Q. supplement 1..V.W. Lee. W. Oxford University Press. 9 – 11 May 2009. D..M.. Journal Animal Science. Prior. B. Panaite. 16. Dua.. J. 53.M. 1841-1856.polyphenols and antioxidant activity in chickens. Trends Food Sci. J. D. Shana. Effects of dietary polysavone (Alfalfa extract) and chlortetracycline supplementation on antioxidation and meat quality in broiler chickens. 2009. Colbert. 2009. 1991. Guteridge. Effect of vitamin E on improving fresh pork quality in Berkshire and Hampshire-sired pigs.. Technol. B. vol. and Ionita. British Poultry Science.. Lee. Atena. X. and Whitaker. 11. 2009..
. G. R. I. Agric. Gaob. J. 2382-2389. Halliwell. The biochemistry and control of enzymatic browning... J. D. X. M. 1995.. & Xua Q.. A..C. T.I. Effects of moderate (5%) levels of linseed in layer diets. 13. K.. 18. N. “The Chemistry Behind Antioxidant Capacity Assays”.A. 439-447.. Food Chem..R. Ou. 2008. Archiva Zootehnica 12:3. 80. F.F. K. 9. F.. 12. caulis and herba. 59(6). Agric. 6(6). 25-31. 56(5). and Hopia. M. Jang. L. M. Lehtonen.. J.Criste. Criste. Hasty. Shin.. K.. Antioxidant Activity of an Ultrafiltration Permeate from Acid Whey.D. See. Khattak. J. B.. Tonga. 1285–1292.. Lia. J. 2005. Cherian. M. D.64. 15.L. World’s Poultry Science Journal. 8. E.. 307–316. 109.. P. Liu. A.. 4947–52. Oxford. Donga. 87. Effect of Ligustrum lucidum and Schisandra chinensis on the egg production. 302-309. Pricop. Z. T. D. Huang. 3230–3237.. Antioxidative Potential of Raw Breast Meat from Broiler Chicks Fed a Dietary Medicinal Herb Extract Mix. 2007. 2011. 87. Chena. Archives of Animal Nutrition. 14th Panhellenic Pharmaceutical Congress. Poultry Science. Sua. J Agric Food Chem.. J. D.. 6. Stoica. A. Food Chem..
22. Raducanu. A. (B+.S. N. H. and Fayek. ISSN 1454-7368). X. Effect of using 250 ppm supplemental vitamin E in omega-3 PUFA-enriched diets for layers. 598-603. M.64. C. European Food Research and Technology. Menten.. T. K.Academia Agricola din Sofia (ISSN 0514-7441).H.19. 2008. 91-95. Meluzzi. QUALITY.. 47-52. D. 20. Panaite. C. Criste.. Bercaru. S. A. C. Schaich. R. Radwan. 59(4). C. R. Rachieru.. T.6] 24. Revista de Chimie Bucureşti. 21.. Panaite. 655-661. Total Antioxidant Activity.. U. L. R. F.. Buissa.. Bulgaria.. 392-394.. Diaconescu.. Parpinello. [ISI 0. ISSN 0034-7752. Ciurescu. Biol Environ Sci. 2005... Yesilbag. 2008. The effect of some mineral and phytogenic additives.. rich in polyphenols.. Food Chem. Ciurescu. supplement 1... Nicorescu. 5(13). Popa et al. 30. Hassan. 4290-4302.17). V. A. Serum Biochemical Profile of Broiler Chickens Fed Diets Containing Rosemary and Rosemary Volatile Oil. and Criste. vol.. Papuc.P. Alencar. Panaite. Ion Ionescu de la Brad. 232.. and Criste. W. D. Untea. 13-15 Noiembrie. 51. Effect of Natural Antioxidant on Oxidative Stability of Eggs and Productive and Reproductive Performance of
. M. V.M. Durdun. F. D.M. J. A.. T. 3. Journal of Animal
Science.. Sirri. Tallarico.. F. 2008... A. Prior. I International Congress on "FOOD TECHNOLOGY. 2011.. G. published on line 23 februarie 2011. 2007. Revista de Chimie.. R.. Int. pag. D. R.. Panaite. R.. 2011. Qota. 920-924.. Ionescu. Phenols and Flavonoids Content of Several Plant Extracts. Serdaru. Wu. and Eren. 2011.. R. A. 61(10). Crivineanu. N. R.. ISSN 0034-7752. 27.D. 2010. Raducanu. upon lipid peroxidation process..D. Agric. Papuc. Antioxidant activity of polyphenols from Sea buckthorn fruits (Hippophae rhamnoides). M. “Standardized Methods for the Determination of Antioxidant Capacity and Phenolics in Foods and Dietary Supplements”. 115..C... Nicorescu. AND SAFETY". Iasi. E. 26.M. 23-30.. [ISI 0. Rachieru. and Skibsted. 53.. N. Lucrari stiintifice seria Zootehnie. J. Racanicci. T. Cismileanu. Criste.A. pp. 29.. Data on yolk vitamin e and cholesterol from layers fed dietary oil extract of tagetes patulus. 2008. C... C.L. C. 2008. Comparative data on the use of different selenium sources in the layer diet. M. J.. C. World’s Poultry Science Journal (ISI 1. 28. Versari. D. si Criste. Mate (Ilex paraguariensis) as dietary additive for broilers: performance and oxidative stability of meat..6] 25.. Polat. 39(1).. 2006. Ed.. Novi Sad. Food Prop. „Sensory evaluation of egg products and eggs laid from hens fed diets with different fatty acid composition and supplemented with antioxidants” Food Research International. J. Studies on the effect of the dietary selenium source for fattening pigs (60-110 kg) on pig performance. SERBIA 23. L.
2011. 36. Colindres. Nutr. Anim. 34. Raes. Morrissey.. 43. Panaite. Arnouts. Brewer. 1121-1149.. Abd El-Salam A...... and De Smet. K. L... R. 2008. 38. Raes. A. frozen pork patties. refrigerated beef and pork. P. R. F.T. ACS Symposium Series 956.
. Raes. reproductive performance and egg quality during storage and its content of cholesterol. 7 (2). S. A. Arnouts. Lipid and protein oxidation of broiler meat as influenced by dietary natural antioxidant supplementation. S. 87. 95. De Smet. S. 285–290. A. Panaite.. Sasse..V. J Food Qual. Physiol. Buckley. Huyghebaert. Sci.. S282–8. 30 (4). Hassan. Haak. K.. 2008.. E.. Effect of natural antioxidants on oxidative stability of cooked. used in fodder premixes to obtain consume egg with new qualities of functional food. 2008.. Tang. Ho.. Brewer.. 3(12). International Journal of Poultry Science. J Food Sci. S.D. 74(1). R. Editors... Tudor et all. Effect of natural and synthetic antioxidants on oxidative stability of cooked. S. G. M. 37.. 20-23 August. Mazilu. Smet. Wiley-Interscience. K.Timisoara. J Food Sci. 2008. P. M. Criste... Brewer. Antioxidants of plant origin. 2007. 31.. Huyghebaert. Poult Sci. Huyghebaert.Laying Hens. American Chemical Society. Effect of dietary antioxidant supplementation on the oxidative status of plasma in broilers. Dietary tea catechnins and iron-induced lipid oxidation in chicken meat...46. L.. 72. D. Kerry. 1682–1688. S..173–88. Criste. Tamas. Sinaia. Ntawubizi. F. Vossen. S... J. M.. D. liver and heart. 2010.S. T. M. vacuum-packaged beef and pork. Poult Science.. T. 33. 1682-1688. T. 56 (1). 87. De Smet. Rojas. 41(1). M. Acta Medica Marisiensis. 134-150. Haak. 41. Rojas. Effect of adding green tea and its aqueous extract as natural antioxidants to laying hen diet on productive. M. Anim. Z. Ionescu. Egypt. Shahidi. 2009. A. Ropota.... 2010. G. 2000. Contribution to phytobiological study of Urtica dioica L... M. L. “Wheat Antioxidants”. S. 2007. “Antioxidant Measurement and Applications”. Romania 39. Washington. Smet. J.. 198-205.. 32. 42. Editor.. 40.. Adaptation of the quality control of the polyunsaturated fatty acids and vitamin E-enriched feeds to the European StandardsLucrari Stiintifice zootehnice si biotehnologii. 541-547... K. 35. Zeinab Abdo. K.. Poult. Rachieru. P. G. Effect of natural antioxidants on oxidative stability of frozen.. S30–5. Arnouts. A. Smet. 2007. K. Lipid and Protein Oxidation of Broiler Meat as Influenced by Dietary Natural Antioxidant Supplementation. First Conference with International Participation. 43 .S. E. Meat Science 56.. 2008a. Yu. Sheehan. and Helmy S.. C. Urticaceae.
working techniques and economic analyzes of evaluation of effects of using some by-products of vegetal origin as antioxidants in the feed of laying hens and broiler chickens Physical and chemical characterization of by-products (BYPROD) of vegetal origin and valuing their efficiency as feed additives with antioxidant role in laying hens and broilers feed. to determine the effects of by-products (BYPROD’S) used as antioxidant additives in broilers feed Dissemination and exploiting of the obtained results Involved partners Start End month month (1 . to determine the effects of by-products (BYPROD’S) used as antioxidant feed additives in laying hens feed Experimenting.. Multidisciplinary studies regarding methodologies. P3. “in vivo”. P1. “in vivo”. P2. P3. P4
. P2. P1. P3. P4
CO. n-1) (2 . P2. P4
CO. P1. P3.Table: Phase list Phase Phase title no.. P4
CO. P3. P4
CO. P2. P1.. P1. P2. enriched in ω3FA Experimenting..
chicken meat) relatively cheap. on lipid peroxidation of PUFA from poultry feed. CO – Documentation on the norms and regulations concerning the use of phyto-additives in animals feed Task 1. 1 Phase title Multidisciplinary studies regarding methodologies.2.Table: Phase description (for each Phase-max 2 pages) Phase no.1. with antioxidant potential. with compliance with the legal regulations in the domain : P1 – Will perform the technical and economic feasibility study. in the context of regulations in the domain of feed additives Description of work (possibily broken down into tasks) and role of participants Task 1.3. Study of technical and economic feasibility on the opportunity of valuing vegetal byproducts from food industry. added to the rations of poultry (laying hens and broilers) fed with rations enriched with omega-3 polyunsaturated fatty acids (ω3FA) . which attains the estimated nutritional needs and food preferences of healthy and dynamic consumers.The technical and economical analysis on the opportunity of practical application/ valuing byproducts resulted of food industry and food supplements industry of the partner economic entity (P1) as feed raw materials for poultry. in poultry nutrition.Obtaining food (egg. working techniques and economic analyzes of evaluation of effects of using some by-products of vegetal origin as antioxidants chickens Involved partners Personmonths Start month End month CO 6 May 2012 July 2012 P1 16 P2 2 P3 12 P4 1 Total 37 in the feed of laying hens and broiler
Objectives . as additives with antioxidant activity. Methodologies and modalities of characterization and assessment of the antioxidant
. safe. on the antioxidant status of poultry and on the oxidation processes of lipids and proteins from the poultry meat. P2 – Study on the products of vegetal/botanical origin used as antioxidants in animal nutrition. P3 – Study on the component chemical substances which give the antioxidant characteristic of vegetal materials Task 1.Setting the methodologic framework for evaluation of the vegetal by-products potential. nutritionally rich. Screening of vegetal by-products with antioxidant capacities: CO – Updating published data on the nutritional solutions of feed enrichment for laying hens and broilers with omega-3 polyunsaturated fatty acids. organs and eggs.Evaluating the effects of using in poultry feed by-products of vegetal origin. . and the field regulation) .
Financial private contribution (%) at the project – 10.4. experimental protocols – month 3. eggs). kidney and food of animal origin (meat.9% from the total budget of the phase – month 1-3 . feed raw materials and fodder. liver. list of minimum 6 vegetal BYPROD proposed to be physically and chemically characterized from the perspective of the antioxidative potential and conditioning for embedding in feed– month 3 Results listed in annex 6 . list of feed raw materials used to enrich poultry rations in ω3 FA. CO. P3 –Development of a working methodology to determine components with antioxidant character in vegetal by-products and different types of feed. Working protocol to determine the antioxidant effect of by-products on serum and poultry muscles. study of feasibility – month 2. Task 1. feed and biological samples : P2 – Working protocol to determine the antioxidant capacity in vegetal materials.2 and 1. Deliverables (brief description and month of delivery) Results unlisted in annex 6 List of regulations in the domain of using phyto-additives in animals nutrition – month 1.Value of the financial private contribution at the project – 9828 lei – month 1-3 facility of
. P1 – Setting the terms of conditioning and modalities to include BYPROD in poultry forages.Number of enterprise participant – 1 participant – month 1-3 . P4 – Working protocol to determine the antioxidant capacity from samples of brain. Analysis of by-products resulting from production activity of P1 that would arise from raw materials with antioxidant potential: CO.capacity in vegetal by-products.3 and setting the list with by-products (titled as BYPROD) which will be characterized in phase 2. P1 – Summarizing conclusions of studies from Tasks 1.
Phase no. primary products of lipidic peroxidation (lipidic peroxides. conjugated dienes) and secondary products of lipidic peroxidation (compounds which react with
. enriched in ω3FA. chemical composition and the potential as antioxidant agents for BYPROD proposed for study: P1 Conditioning of some experimental lots of BYPROD in order to characterize and include in batches of experimental feed CO –
Determination of maintenance of the quality in time (during 1 month). Evaluation of antioxidant effect of BYPROD incorporated in feeds enriched in ω3FA: CO –From the 18 feeds prepared at the level of laboratory and stored at room temperature (15 0C). enriched in ω3FA
Involved partners Personmonths Start month End month
CO 11 August 2012
Objectives . selected to be used as antioxidant agents in poultry feed.3. P2 . Task 2. of the 9 types of feeds for hens.Elaboration of the set of 9 feed recipes for hens and 9 feed recipes for broilers this way: FC recipe.2. CO+P1 – Setting of a consensual level of BYPROD (chosen at the end of the preceding phase) inclusion in poultry feed. Description of work (possibily broken down into tasks) and role of participants Task 2. at the laboratory level. FAOS recipe and 6 experimental feed recipes optimized against FC recipe (enriched in ω3FA ) by adding at each variant one from the 6 assortments of BYPROD. Determination of the active compounds. .Valuing of nutritional quality of BYPROD . P2. for a month period. Fabrication.Valuing of by-products (BYPROD) potential. weekly. samples will be drawn out for comparative determination of fat degradation indexes (peroxide index. for laying hens and broilers.Determining. on the same type and number of feed samples as CO. Kreiss reaction). Elaboration of feed recipes for hens/broilers and their preparation at the laboratory level: CO. CO. respectively 9 feeds for chicken broilers (structured on phases of growth and finishing) Task 2.Development of a nutritional strategy to optimize feed recipes. Phase title
2 Physical and chemical characterization of by-products (BYPROD) of vegetal origin and valuing their efficiency as feed additives with antioxidant role in laying hens and broilers feed.Determination of antioxidant capacity (AO) of the by-products and determination of microbiological load .1. P3 – Qualitative determination (identification) of active compounds as antioxidants.
7.3. in feeds. Selection of BYPROD which will be studied by ”in vivo” experimentation on hens and. broilers. in time.Qualitative determination concentration of active antioxidant components in those 18 feed samples collected by CO. classifying BYPROD. Task 2.Financial private contribution (%) at the project – 20.Value of the financial private contribution at the project – 35363 lei – month 4-8
. Task 2. respectively. arranged based on antioxidant capacity (AO). classifying BYPROD according to their manifested antioxidant capacity. feed recipes enriched in ω3FA for hens (FL_ω3FA). Will be selected the first two BYPROD (titled as BYPROD’S_1 respectively BYPROD’S_2) for embedding in feeds tested in experiments. Determinations concerning microbiological load. the first two will be selected by-products (titled as BYPROD’S) to be used as feed additives – month 8. respectively. 6. progress report –month 8.2 and 2.2% from the total budget of the phase – month 4-8 . Deliverables (brief description and month of delivery) Results unlisted in annex 6 Analysis bulletins – months 5.4.month 8 Results listed in annex 6 .tiobarbituric acid -TBARS).Number of enterprise participant – 1 participant – month 4-8 . P3. CO+P1+P2 summarizing results from tasks 2.5 Annual meeting of consortium CO-presentation of web page. broilers (FB_ω3FA) which will constitute control recipe in ”in vivo” conducted experiments. audit certificate/2012.
respectively 8 feeding weeks. scientifically founded and experimented.Phase no. brain. at hens slaughtering (6 hens/ lot) from the end of the experiment will collect serum. Task 3. “in vivo”. kidney and liver samples. Task 3. diveded in four lots. of vegetal origin. P2 –Achieves microbiological control and determination of antioxidant capacity from raw materials and feed fabricated in each experiment. eggs (18 eggs/lot) for physical and chemical analysis. to determine the effects of by-products (BYPROD’S) used as antioxidant feed additives in laying hens feed
Involved partners Personmonths Start month End month
January 2013 October 2013
Objectives .2. will collect biweekly.Increasing the visibility of competence and expertise of research teams. will follow bioproductive parameters (consumption. 26 weeks age. will collect manure samples after 4. partners in consortium Description of work (possibily broken down into tasks) and role of participants The experiments on animals will be hosted by CO. nutritional and mycotoxicologic quality control of raw materials and feed. health status of hens. Phase title
3 Experimenting. of valuing some renewable natural by-products from local sources . P3 – Determines the content of active antioxidant substances in fabricated feeds Task 3. Fabrication of feeds for laying hens and their quality control: P1 – Ensuring conditioned BYPROD’S (dried and ground) to include in feed.Diminishing of unwanted effects of polyunsaturated fatty acids oxidation from hens rations. BYPROD’S_2. on eggs enriched in ω3FA . starting with the second feeding week. respectively the mixture
. 3.Validation of some innovative solutions. . The experiments conducted on animals are conducted with the agreement of the commission for professional ethics of the institute.Ensuring of natural alternatives. weight evolution). Organizing and conducting 3 experiments on laying hens: CO – will organize 3 experiments. CO – Fabrication of 4 feed variants / experiment in pilot station from IBNA. egg production.1. to the expensive synthetic compounds which are used at present for prevention of polyunsaturated fatty acids oxidation from laying hens feed . each of 8 weeks period.Increasing of antioxidant status of hens and evaluation of oxidative processes at the level of lipids and proteins from poultry meat and organs. carried out in their own experimental warehouses. Each experiment will use 120 hens. Verifying the efficiency of BYPROD’S_1.
Financial private contribution (%) at the project – 16. in group with P1. Deliverables (brief description and month of delivery) Results unlisted in annex 6 Measuring bioproductive effects of experimented feeds -months 12.3% from the total budget of the phase – month 9-18.Registrated patent application – month 18 . determining the proteins solubility and electrophoretic profile of proteins from poultry muscle.6. peroxidase. vitamins and minerals) . fatty acids profile. 10. determines microbiological load from manure. Task 3. P2 – determining the antioxidant capacity from samples of serum. respectively 4 storing weeks) and chemical parameters specific for yolk (fat. determining of primary and secondary products of lipidic peroxidation from meat. 12. glutathion peroxidase.(BYPROD’S_1+ BYPROD’S_2) used in feed rations of laying hens : CO will determine in egg samples (3 eggs/sample) physical parameters (weights. classification of improved diets according to the obtained results in the 3 experiments – month 17. Dissemination of results with relevance for scientific community and which are not subject of documentation for patenting: CO. Task 3.7 Annual meeting of consortium CO+P1+P2+P3+P4 Evaluation of project progress. 13. cholesterol. yolk color intensity and frehness after 2. P3article within ISI quotation revue. 14.National and international mobilities – month 14 x persons 4
. P2 . liver and egg yolk. 14. P4 – determining the antioxidant capacity from samples of brain. glutathion reductase and of the reduced glutathion level from blood. 16. Haugh unit. progress report -month 18 Results listed in annex 6 . Task 3. of the data obtained in tests on preserving the quality of feed batches produced at the laboratory level. analysis bulletinsmonths 9.: Elaboration of documentation for patent application -feed recipe with the best results during the phase.Accepted for publish or published articles in the mainstream journals – month 14 .5. egg shell thickness. evaluation of metmyoglobin and myoglobin level from poultry muscle. Value of the financial private contribution at the project – 663049 lei – month 9-18 . organs and eggs. 16. Task 3. CO and P1.Number of enterprise participant – 1 – month 9-18. 15.communication. identification of occured problems and of modalities of overcoming and solving them.4 Evaluating antioxidant status of hens and oxidative processes at the level of lipids and proteins P2 – determining the activity of antioxidant enzymes catalase. Evaluation of free radicals scavenging activity of egg yolk. liver and kidneys. P4– elaboration of one article for journals with impact factor. pH.
kidney and liver samples. meat (breast and pulp chicken). at the broilers slaughtering (12 broilers/lot) at the end of the experiment will collect serum. brain. P1. will collect manure samples after 4. protein. fatty acids. divided in 4 lots. health status of hens.Increasing antioxidant status of broilers and evaluation of oxidative processes at the level of lipids and proteins from chickens meat and organs . Fabrication of feeds for broilers and control of their quality: CO: Fabrication of the 4 variants of feeds/ experiment in the pilot station from CO-IBNA. vitamins and minerals). sensorial parameters. Task 4. aminoacids.2. BYPROD’S_2. respectively 8 feeding weeks. Verification of efficiency of BYPROD’S_1. “in vivo”. fat. P2 –Accomplishes microbiological control and determination of antioxidant capacity from the raw materials and feeds fabricated in each experiment. will follow bioproductive parameters. nutritional value (dry substance. P2 – activity of antioxidant 29
. cholesterol. 3. healthier and high quality food . Task 4. nutritional and mycotoxicologic quality control of raw materials and feeds. Phase title Involved partners Personmonths Start month End month
4 Experimenting. Each experiment will be carried for 42 days.Obtaining safer. respectively the mixture (BYPROD’S_1+ BYPROD’S_2) used in broilers feed rations: CO: will determine in meat samples (breast and pulp chicken) physical. to determine the effects of by-products (BYPROD’S) used as antioxidant additives in broilers feed CO P1 P2 P3 P4 Total 13 21 4 18 4 60
November 2013 July 2014
Objectives . The experiments conducted on animals are conducted with the agreement of the commission for professional ethics of the institute. 4. P3 – Determines the content of active antioxidant substances in fabricated feeds. Organization and development of 3 experiments on laying hens: CO – Will organize 3 experiments on broilers ongoing in own experimental warehouses.Stimulation of partnerships between research units and private businesses in solving some complex problems Description of work (possibily broken down into tasks) and role of participants The experiments on animals will be hosted by CO. on 240 broilers 7 days of age.Assuring processed BYPROD’s (dried and milled) in order to include them in feed.Phase no.Determination of BYPROD’S effect on feeds and broilers meat stability .Using some natural bioresources of local origin to diminish unwanted effects of fatty acids oxidation from broilers rations .1. ash.
month 27. P3. scientific society at the Faculty of Veterinary Medicine – month 23. P1: dissemination of the results from the previous phase. 1 seminar at the Faculty of Pharmacy – month 24 . classification of the recipes based on bioproductive effects and maintaining the feed quality and meat quality – month 26. 25. P3.5. Results listed in annex 6 . evaluation of the level of metmyogobin and myoglobin from poultry muscles. 26 x persons 4
. report on the project progress .month 19. Financial private contribution (%) at the project – 16. peroxidase.Number of enterprise participant – 1 – month 19-27. 26. data base– months 12-17. Dissemination of the results on used working methodologies: CO: elaboration of articles together with P1. analysis bulletins.seminars at the Faculty of Pharmacy from UMF Bucuresti Task 4. audit certificate /2013 month 20. 25.29% from the total budget of the phase – month 19-27. articles and communications at professional events –months 24. experimental observations and conclusions resulting in microtests –month 26.Accepted for publish or published articles in the mainstream journals – month 24. Task 4. according to their relevance for different categories of beneficiaries Deliverables (brief description and month of delivery) Results unlisted in annex 6 Optimized feed recipes . determines microbiological load from manure. liver and kidney. glutathion peroxidase. 25. experimental report regarding scientific data. by participation at exhibitions organized by the patronage from food industry. unprotected by patent. 26 . liver and meat samples. Value of the financial private contribution at the project – 108048 lei – month 19-27 . solubility of proteins and electrophoretic profile of proteins from chicken musculature. P2. glutathion reductase and of the level of reduced glutathion will be determined from blood. P4 – determination of antioxidant capacity from brain.enzymes catalase.4. P2 + P4– dissemination at the university level by a student scientific society at the Faculty of Veterinary Medicine USAMV Bucuresti. Annual meeting of consortium CO+P1+P2+P3+P4 Analysis of the obtained results and of valuing modalities.National and international mobilities – month 24.
Elaboration of the documentation for patent application of a new feed recipe. Avicultorul. through presentation at the annual meetings with farmers organized in spring at IBNA. roundtable – months 30. P3 – article in ISI journal. Final meeting of consortium: Appreciation of the obtained results. publish 3 articles in the journals The Farmer. enriched in omega-3 fatty acids. CO+P1 elaborates a brochure to promote the results of the project at the level of the business field and government organizations.1. of valuing byproducts from food industry in animal nutrition . Task 5. 32.Phase no. P4 article in ISI journal.4. P1: organizes a workshop. P1 – Elaboration of documentation Task 5. participation at national exhibitions addressed to the 31
. article in ISI journal together with P2. workshop. AGRALIM. of the degree of implementation and elaboration of the final report. Task 5. Dissemination of the results in the scientific field: CO – presenting the results at the international symposium IBNA.2. AGRARIA.Prevention of accumulation of by-products resulted from food industry and food supplements industry Description of work (possibily broken down into tasks) and role of participants Task 5. Lumea Satului.3 Dissemination at the level of the business field and government organizations CO: disseminates project results on the project web page.month 31. Economic analysis of the impact of project at P1 P1 – Elaborating a business plan Task 5. founded and scientifically experimented. brochure for information and awareness of consumers. Phase title Involved partners Personmonths Start month End month
5 Dissemination and exploiting of the obtained results CO 12 P1 12 P2 2 P3 6 P4 2 Total 34
August 2014 February 2015
Objectives -Summarizing and correlation of obtained results in all the phases of the project -Dissemination of the results which are not subject to patenting -Dissemination of the results at the level of national exhibitions dedicated to the economic field -Analysis of the economic results obtained by the economic agent –business plan -Integration of some innovative solutions. 31.5. Poster at the national exhibitions INDAGRA. for broilers: CO. presenting results at symposiums in the field. Deliverables (brief description and month of delivery) Results unlisted in annex 6 Business plan -month 32.
Value of the financial private contribution at the project – 39291 lei – month 28-34 .Accepted for publish or published articles in the mainstream journals – month 29.. 30 x persons 4
Table: Deliverables List Deliverable Deliverable Name No. final report. article etc
Phase no. 30 . technology. audit on the project-months 34 Results listed in annex 6 .
5 6 *
of Phase delivery date (1 . n) Phase 1 – month 1-3 Number of enterprise Phase 2 – month 4-8 P1 . audit certificate/2014.
.National and international mobilities – month 29.business field..Registrated patent application – month 29 .Financial private contribution (%) at the project – 16.Number of enterprise participant – 1 – month 28-34 .months 32.42% from the total budget of the phase – month 28-34 .Hofigal participant – Phase 3 – month 9-18 1-5 1 partcipant Phase 4 – month 19-27 Phase 5 – month 28-34 Phase 1 – month 1-3 Financial private Phase 2 – month 4-8 Financial private contribution (%) at Phase 3 – month 9-18 1-5 contribution (%) the project Phase 4 – month 19-27 Phase 5 – month 28-34 Phase 1 – month 1-3 Value of the financial Phase 2 – month 4-8 Value of the financial private contribution Phase 3 – month 9-18 1-5 private contribution at the project Phase 4 – month 19-27 Phase 5 – month 28-34 Accepted for publish Accepted for publish or published articles Phase 3 – month 14 or published articles in in the main Phase 4 – month 24-26 3-5 the main international international Phase 5 – month 29-30 scientific flow scientific flow National and National and Phase 3 – month 14 international international Phase 4 – month 24-26 3-5 mobilities mobilities Phase 5 – month 29-30 Registrated patent Registrated patent Phase 3 – month 18 3. 5 application application Phase 5 – month 29 according to Annex 6 – results indicators of the Programme (patent.
which will be responsible for daily management and administration of the project work. Transmitting documents and information connected with the Project to any other Parties concerned. Analyzing the performance indicators. taking into account the evolution of the context in which the project is carried out.
. Obtaining audit certificates by each of the contractors. Agreement. will be composed by the Project Manager and the Project Responsible of the Parties. The responsibilities of the Management Committee will be: The overall direction of all activities. implementation and oversight an appropriate Intellectual Property Strategy. Monitoring compliance by the Parties with their obligations. The Project Coordinator will oversee The Management Committee (consisting in Project Manager and Project Responsible of the Parties) and The Administrative and Financial Service (department of Coordinator Institution). The Administrative and Financial Service will consolidate the project reports provided by Project Responsible of the Parties and the Project Coordinator. reviewing to verify consistency and submitting reports and other deliverables (including financial statements and related certifications) to the Contracting Authority. Management structure and procedures The Project Coordinator will act as overall leader of the project.1. The Management Committee will be responsible for all strategic and administrative decisions of the project. with the coordinator having the casting vote. To assist the coordinator in approving publication of scientific articles and presentation of results at national and international meetings. and will be responsible of the financial administration and Project meeting administration. Collecting. Project Coordinator main duties will include: Chairing the Management Committee. The Management Committee makes decisions upon consensus. however. Coordination of knowledge management and other innovation-related activities. To agree the scientific details of the work program. Management and distribution of financial support amongst the partners. A meeting of this committee has already taken place and was responsible for the drafting of this application. being the intermediary between the Parties and the Contracting Authority and will perform all tasks assigned to it as described in this Consortium Agreement. Submitting financial statements and receiving in trust for the consortium all payments from the Contracting Authority and distributing them among the contractors according to their decisions. ensuring the transfer of knowledge to the end users. Active involvement in the promotion of the project. Overseeing the efficient distribution of funds to the consortium members as per the agreed budget. The committee will meet at least twice a year throughout the project. in the unlikely event of no agreement being reached.Implementation 2. each member will have one vote for decision making. Chaired by the Project Coordinator.
In 2011. the institute has a good logistics for knowledge transfer. an experimental feed mill. Individual participants Coordinator of the project (CO): NATIONAL RESEARCH & DEVELOPMENT INSTITUTE FOR ANIMAL BIOLOGY AND NUTRITION (IBNA BALOTESTI). patents
2. Optimizarea structurii de nutreţ combinat sub raport vitamina E / seleniu / acizi graşi în vederea îmbunătăţirii calităţii carcaselor. Some recent projects include: Eggshells processing and eggs with functional food properties production (EUREKA Technological Area. new functional food on Romanian market (CEEX national programme/ 22/2005). The Institute is involved in all phases of the project. arable land & facilities for producing experimental forages.3 Nutrition and Health). experimental farms (main farm animal species).Contracting Authority CO Administrative financial service Management Committee
Project development Multidisciplinary studies Fundamental research Experimental reasearch
Identification and characterization of sub-products which will be included as antioxidant feed additives
P1 CO P3
In vivo experiments. IBNA has complex researchdevelopment activities in the field of the biology and nutrition of all species and categories of farm animals (from research to extension).2. The team is currently involved in several projects relevant for this project proposal activities and has experience in FP/6/7 and Eureka projects. la puii broiler (AGRAL national programme 132/ 2002) Role IBNA is project’s coordinator. IBNA has one of the best research
infrastructures in the whole Romanian agriculture research: facilities for animal nutrition physiology. Also.is the National Institute in the field of animal science in Romania. The determination of sub-products’ effects Dissemination of the obtained results Scientific papers. Optimization of nutritional factors to obtain enriched egg consumption in polyunsaturated ω: 3 fatty acids . the study of the new
. 8. the institute has 30 projects (25 national and 5 international ones) from which 17 as coordinator. having direct responsabilities concerning: the development of nutritional strategies.
senior resercher (I). Partener 2 (P2): UNIVERSITY OF AGRONOMICAL SCIENCES AND VETERINARY MEDICINE BUCHAREST. She has experience in coordinating national research projects (8 projects as project manager. major participation in other ten projects) and work experience in FP6. author and co-author of over 65 patents and inventor certificates. provides and produces finished products at the pilot station. will provide the necessary quantities of plant byproducts during all stages of the project. coordinates the technical. cosmetics. works in the field of those vegetal extracts that can be
. quality check of feeds and animal products. head of Department of Chemistry and Physiology of Nutrition in IBNA Balotesti. eggs and poultry manure. researcher for the improvement of existing technologies. development of nutritional solutions to limit the polluting risks. feed. 32 years. FP7.(USAMVB) . achieves microbiological control in feed. In order to attain the quality objectives. Dr Viorica Tamas (PhD). 56 years. Role: USAMVB will participate in all phases: evaluating the effects of using in poultry feed by-products of vegetal origin. senior researcher (III) on poultry nutrition physiology. with antioxidant potential. She is the project manager. veterinarian. nutritional solutions for functional foods. Role: HOFIGAL SA. chemist. Research Director in Hofigal. administrative and financial activities of the project. USAMVB associates educational activities with research activities in the following fields: processing of animal productions. and teas. USAMVB. 33 years. Researches in the last 5 years are focused on antioxidant activity of plant polyphenols. responsability of project management within IBNA. food safety and security. Her scientific fields of interest are: poultry & pig nutrition. dietary supplements. spectrometric determinations. Processed by-products will be subject to quality control and analysis using modern means. eggs and broiler meat that were studied. Tatiana Panaite (PhD). Key persons: Camelia Puia Papuc (PhD). on lipid peroxidation of PUFA from poultry feed. senior researcher (III). gemoderivative. specialist in Eugenia
biotechnology .solutions by organizing experiments on broilers and laying hens which are developed in their halls. Andreea Cozea (PhD). veterinarian and biologist. 56 years. chemist. is a senior researcher and Professor in Biochemistry Department. Faculty of veterinary medicine.C. 29 years.HOFIGAL EXPORT – IMPORT S. 34 years. effective for this purpose. Key persons: Rodica Diana Criste (PhD in animal science). USAMVB is an approved state university in agriculture field. the determination of the nutritional quality of the by-products. 55 years. 32 years. EUREKA projects. senior researcher. Key persons: Natalita Bordei. Valentin Razvan Nicorescu (PhD). Untea Arabela (PhD student). Mircea (PhD student). and phytotherapeutic galenical products in Romania. Partner 1 (P1) : S. made in the form of pharmaceutical products.A (HOFIGAL) is one of the famous brand names when it comes to homeopathic.
Maria Lidia Popescu (PhD). senior lecturer at the Department of Pharmacognosy Phytochemistry Phytotherapy. which is currently running. Key persons: Dr Andrei Florin Danet (PhD) (65 years) analytical chemistry scientist. and with P2 in SUPLIBOR national project (Transdisciplinary research concerning on the essentiality and the additional levels of boron in broiler meat and piglets) project. Consortium as a whole The project is vast and ambitious and its accomplishment is guaranteed by the professional and technological capacity of the consortium.3. 45 years.
. in medical domain and related fields. Their very
fruitful collaboration from these projects is one of the reasons why these partners got together again for this project proposal. biochemist. yolk egg samples. Role: UMF is involved in activities which aims: qualitative chemical analysis for vegetal raw materials and feed in order to identify antioxidants. UMF is a higher-education institution. member in 6 national projects. Ligia Elena Duţu (PhD). senior researcher in analytical biochemistry. 20 years work experience in the field of pharmaceutic education. The team of the present project contains 6 academics involved in several projects. in FITOMIN national project (The reduction of the level of mineral trace elements in pig manure by the replacement of chemical premixes with phytogenic additives and enzymatic preparations.(UB). Faculty of Pharmacy . 39 years. scientific explanation (based of chemical composition) of pharmacologic activity of herbal products. 43 years. He was involved in 6 European projects. P1 and P2 have already been partners before. Partener 3 (P3) UNIVERSITY OF MEDICINE AND PHARMACY „CAROL DAVILA“Bucharest. UB is one of the biggest university in Romania. Role: UB will perform determination of antioxidant capacity of the brain and liver poultry samples. (Optimization of the nutritional factors for the production of ω : 3 polyunsaturated fatty acidsenriched egg. way of promoting the sustainable agriculture). Dr Valentina Popa (PhD). member in 8 national projects. as new functional food for the Romanian market). senior lecturer and the head of the Department of Pharmacognosy Phytochemistry Phytotherapy. it will contribute to different dissemination activity. concerning the following: introduction/evaluation of new herbal raw materials in therapy. 41 years.used in veterinary medicine. Key persons: Cerasela Elena Gîrd (PhD). 2. The team designated for developing this project was involved in several national projects recently finalized. The project coordinator (CO). ensures project promotion and dissemination of the project’ results. He published more than 100 papers in different branches of analytical chemistry. Faculty of Chemistry . classified as a university with advanced research activity and education. Partener 4 (P4) BUCHAREST UNIVERSITY.(UMF). CO has also been partner with P1 in OMEGANUTRIOSAN national project.
2.4 and 5. All the partners participate in the dissemination activities and in the consortium meetings.3 4. P2 – USAMVB has a team of teaching staff involved in research focused on the antioxidant activity of plant polyphenols. Hofigal is involved in all stages.1 3. laboratories for chemical and biochemical investigations (some of which are ISO 17025 accredited).5
5.4 3.3. with antioxidant potential.2 2.4 2. is involved in all phases of the project and in all its tasks. This department has the proper analytical equipment to assay the by-products (processed by P1) and the active antioxidants from the compound feeds (manufactured by CO).1 2.2 1. P3 – UMF has a team consisting of teaching staff from the Department of Pharmacognosy Phytochemistry Phytotherapy of UMF.3 5. P2 and P3.6 3.5
3. eggs and poultry droppings.1 5.2 4.2. The working team is multidisciplinary and it consists of specialists in animal nutrition. environmentally controlled experimental houses fitted with new (2008.2 3. The table below shows the interconnection between partners throughout the project.7
4. Phase 1 Task CO P1 P2 P3 P4 Task CO P1 P2 P3 P4
1.CO – IBNA.3 3. 2009) cage systems for poultry and good logistics for knowledge transfer. properly endowed with research and analytical equipment and instruments. These by-products are transferred as analytical samples to CO. The Biochemistry Department of USAMVB has all the specific and necessary equipment and instruments to evaluate the effects of using in poultry feed by-products of vegetal origin. who have the necessary expertise to determine the antioxidant capacity in the biological samples collected by CO during the trials on layers and broilers. has all the adequate infrastructure needed for this kind of projects: pilot mill for compound feeds manufacture. 5 3. it also performs the microbiological control in feed. 3. P1-Hofigal has a strong research department.2 5. most of whom are PhD or PhD students.3 1.1 1.3 2.4
. having responsibilities particularly in the tasks concerning the by-products which resulted from the processing of the plants grown in its own greenhouses. except 1. and these dried and grounded samples will be send it to CO which will use it in the formulation of the compound feeds for poultry. on lipid peroxidation of PUFA from poultry feed. P4 – UB consists of teaching staff from the Faculty of Chemistry.
3. blender. turbidimeter. CO performs activities which involves: identification and characterization of vegetable byproducts. egg shell thickness gauge. P2 will involve the project team consisting of 6 researchers and will use the existent equipment: ovens. centrifuge. Resources to be committed CO . deepfreezer.To achieve multidisciplinary studies framed in the first phase of the project and dissemination of results. coling heating thermostat. egg shell analyzer. nutritional and mycotoxicological quality control of raw material. To conduct these activities all 7 key persons will contribute. laboratory fridges.To achieve multidisciplinary studies and dissemination of results. 3 workers. chemiluminometer. P1 will involve 7 key persons and the existent computerized systems. P1 . P2 . athomic absorption spectrophotometer. P2 will carry out activities involving the determination of by-products and feed antioxidant capacity used in experiments and determination of microbiological load. in the pilot station for compound feeds.To achieve multidisciplinary studies and dissemination of results.To achieve multidisciplinary studies and dissemination of results. centrifuge. rotary evaporator. pH-meter. 3 and 4. These will be manufactured within the coordinating institution. automatic soxhlet extractor. analytic balance. laboratory mill. rotary-evaporator. analytical balances. 4. calcination oven. For carrying out these activities will be involved 10 researchers. In phases 3 and 4 will be produced batches feed necessary for laying hens and poultry experiments. system of video-documentation in gel. spectrophotometer UV-Vis-NIR. determination in egg and meat samples of physical and chemical parameters. 4 laboratory assistants. thermostat. P2 will involve 6
. 3GB DDR3. To achieve the objectives. microcentrifuge. complete ELISA line.There will be involved 4. P3 . CO will use: 6 key persons and computerized systems. FOSS systems. pH-meter. P2 will involve 3 key persons and the existent computerized systems. P1 must acquired PHOTOCHEM analyzer within the project and the interpretation of data analyzer will be made by purchasing a system desktop Serioux Explorer V8 Intel Pentium Dual Core E5700 3 GHz. 2 technicians. and it will be used experimental and digestibility cages for poultry. freezers. 500GB 32MB. vertical freezer. In phases 2. 2 HPLC systems for vitamins and amino acids determinations. For rapid analysis of antioxidants and free radicals.2.4. magnetic agitators. UV lamps. 3 and 4. homogenizer. electrophoresis device in vertical gel. It will use existing equipment in the chemistry laboratory: GC for fatty acids determinations. water bath. electrophoresis device in horizontal gel. 4 technicians. spectrophotometer. nise. automatic potentiometric titrator. In phases 2. In phases 2. P1 will realize the conditioning and the assurance of the experimental groups of BYPROD'S.
Deliverables (listed in annex 6): the value of the private (HOFIGAL) financial contribution within the project is 9828 lei. vacuum-pump. To achieve the experimental determinations and to elaborate the conslusions. a chemiluminometer. setting methodologies and modalities to determine antioxidant effect of by-products on feed. In phases 3 and 4. 3 and 4. poultry organisms and evaluating oxidative processes at the level of lipids and proteins from poultry meat. Phase 2 Physical and chemical characterization of by-products (BYPROD) of vegetal origin and valuing their efficiency as feed additives with antioxidant role in laying hens and broilers feed. will be made determinations concerning their nutritional value. glass filter holder assembly with accessories. spectrofotometer UV-VIS. analytic and precision balance. electric sand-bath.In this phase is aimed to create the technical and logistic framework. electronic balance. P2 will determine active compounds as antioxidants in vegetal by-products and will produce the feed. P4 -To achieve multidisciplinary studies and dissemination of results. P3 will involve a team of 6 persons and will use existing lab equipment: chromatographic system. pharmaceutical sieves with accessories.5. flask heaters with accessories. where will be carried out the project activities concerning: nutritional integrative solutions of optimizing feed recipes for laying hens and broilers by valuing some natural vegetal by-products of local source from the perspective of diminishing unwanted effects of oxidation of fatty acids from poultry rations. analytical balance. from Hofigal. liver and meat samples. fluorimeter.key persons and the existent computerized. 2. rotary-evaporator. antioxidant capacity. autoclaves hood with laminary flow. electric water-bath. active
. In phases 2. we plan to have applied interdisciplinary research activities during 5 phases: Phase 1 Multidisciplinary studies regarding methodologies. P4 will involve 2 key persons and the existent computerized systems. TLC plate heater with accessories. pH-meter. ultrasonic bath with accessories. Also it will be setting the list with 6 by-products (titled as BYPROD). electrochemical equipment for potentiometric. amperometric and voltammetric determinations. which will be characterized in phase 2. enriched in ω3FA From the 6 BYPRODs chosen at the end of the first phase. binocular microscop with digital photo camera. working techniques and economic analyzes of evaluation of effects of using some by-products of vegetal origin as antioxidants in the feed of laying hens and broiler chickens. laboratory oven. centrifuges. Methodology and associated work plan: In order to accomplish these general objectives. For phytochemical analysis P3 needs to purchase: hot air oven with accessories. conditioned by P1. A team of 2 researchers will collaborate and will use the equipment: spectrophotometers with optical fibers. P4 will determine
antioxidant capacity of brain. organs and eggs. supercritical fluid chromatography and extraction system. moisture balance.
liver. 4 variants of feeds differentiated as follows: FL_ω3FA (with ω3FA but without BYPROD’S ) and 3 experimental variants which are differentiated from variant FL_ω3FA by the levels of BYPROD’S_1 (experiment 1). to determine the effects of by-products (BYPROD’S) used as antioxidant feed additives in laying hens feed. hens and broilers. and the concentration of the active antioxidant components. glutathion reductase and of the level of reduced glutathion will be determined from blood. (iii) 6 feeds structured as FC but in which will be embedded. To validate innovative solutions of using BYPROD’S as antioxidant feed additives efficient in laying hens rations enriched in ω3FA (FL_ω3FA).compounds as antioxidants. registrated patent application . 2 articles in the mainstream journals . at the level of laboratory batch: (i) a standard feed. will be made comparative determinations regarding maintaining the quality in time (during 1 month). with the best results. At the end of the phase will take place a first consortium meeting. distributed in 4 lots/ each experiment). for ”in vivo” experimentations in the next 2 phases. brain and kidney samples. national and international mobilities( month 14 x persons 4) Phase 3 Experimenting. in this phase will be conducted 3 experiments on laying hens (120 hens. liver and kidney. BYPROD’S_2 (experiment 2). At the end of each experiment will be slaughtered 6 hens/lot and will be collected serum. From the samples of eggs and organs will be determined primary and secondary lipidic peroxidation products. For each. based on the feed recipes. Will be fabricated. On the basis of the obtained results will be able to select two by-products (titled as BYPROD’S_1 respectively BYPROD’S_2). for each experiment. peroxidase. Activity of the antioxidant enzymes catalase. After summarizing the results obtained in the three experiments will be elaborated documentation for patenting the feed recipe with the most efficient action in terms of eggs enrichment in ω3FA under the conditions of preventing emergence of oxidation reactions in feed
. Will be collected also manure samples which will be microbiologically analyzed. glutathion peroxidase. During the experiments will be followed bioproductive parameters and physical and chemical parameters of reference for assessing eggs quality. will be prepared. at a level of exploration. one of the 6 BYPROD studied previously. (ii) two types of feed enriched in ω3FA (by a raw oleaginous material which will be decided depending on the disponibility on the local feed market) from which one type of feed without antioxidant (titled as FC) and a type of feed (titled as FAOS) with one synthetic antioxidant (vitamin E) . BYPROD compounds thus characterized will be taken into account for elaboration of a set of 18 feed recipes (9 for laying hens and 9 for broilers). a mixture between BYPROD’S_1 + BYPROD’S_2 (experiment 3) added to the rations. Deliverables: the value of the private (HOFIGAL) financial contribution within the project is 663049 lei. “in vivo”. microbiological and mycotoxicologic load. From the 18 variants of feeds fabricated at the level of a laboratory.
registrated patent application national and international mobilities( month 29. to determine the effects of by-products (BYPROD’S) used as antioxidant additives in broilers feed. From the meat and organs samples will be determined primary and secondary products of lipidic peroxidation. The level of BYPROD’S added will not be differentiated between the growing phases. Will be collected also manure samples which will be microbiologically analyzed. During experiments will be followed consumptions and gains. divided in 4 lots. problems which occur and solving solutions. After summarizing the obtained results in the three experiments will be elaborated patenting documentation of the feed recipe with the most efficient action. 2 articles in the mainstream journals. Deliverables: the value of the private (HOFIGAL) financial contribution within the project is 39291 lei. Each experiment will be carried for 42 days. 2 articles in the mainstream journals . both in feeds and in meat. BYPROD’S_2 (experiment 2). The 4th phase has a development algorithm similar with that from the first part of preceding phase. During the phase will be conducted 3 experiments. national and international mobilities( month 24 x persons 4) Phase 5 is for dissemination and exploiting of the obtained results. liver. By the activities of this phase is followed the validation of innovative solutions of using BYPROD’S as efficient feed antioxidant additives in broilers rations enriched in ω3FA (ratia FB_ω3FA). 4 variants of feeds: variant fabricated by recipe FB_ω3FA (without BYPROD’S ) and 3 experimental variants which are differentiated from variant FB_ω3FA by the level of BYPROD’S_1 (experiment 1). At the end of each experiment will be slaughtered 12 chickens/lot and will be collected meat (breast and calf). Deliverables: the value of the private (HOFIGAL) financial contribution within the project is 663049 lei. stage of project progress. mixtures of BYPROD’S_1 + BYPROD’S_2 (experiment 3) which are added to the rations. Will be fabricated.3 x persons 4)
. evaluation of the metmyogobin and myoglobin level from the poultry musculature. Deliverables: the value of the private (HOFIGAL) financial contribution within the project is 108048 lei. in terms of chicken meat enrichment with ω3FA in conditions of prevention of the occurence of oxidation reactions of polyunsaturated fatty acids. 1registrated patent application . national and international mobilities( month 14 x persons 4) Phase 4 Experimenting “in vivo”. Will be also done: determinations on the proteins solubility and electrophoretic profile of proteins from chicken muscles. brain and kidney samples. the basic structure will be established according to the growing phases. on 240 chickens 1 days of age. In the case of recipes for chickens. for each experiment. At the end of the phase will be conducted the consortium meeting which will analyze obtained results.and egg by using a BYPROD’S. serum. 2 articles in the mainstream journals.
3 A III. IV Trim.3 A IV.5 A III.4 A IV.3 A I.5 A III.2 A III.3 A V. III 2014 – 2015 Trim.4 A V.4 A II.2 A V.2 A II.2 A IV.6 A III.3 A II.1 A II.1 A I.Gantt Diagrama
Etapa Categ. I IV
2012 Etapa I 2012 Etapa II
2013 Etapa III
2013 / 2014 Etapa IV 2014 / 2015 Etapa V
Key persons list (their CVs are uploaded on the web platform) Name surname* Coordinator (CO) Criste Rodica Diana and Scientific title CS 1 Phase Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Untea Arabela CS 3 Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Olteanu Margareta CS 2 Phase 1 Phase 2 Phase 3 Person-month 1 2 3 3 2 1 2 3 2 2 1 2 3 42
.5 Trim. Trim.5 A V.1 A IV.4 A III. Activ. III Trim. III Trim . I 2013 Trim . A I. II 2012 Trim.2014 Trim. Trim I .1 A V.7 A IV.1 A III. II Trim. II Trim.2 A I.4 A II. IV 2013 .
Phase 4 Phase 5 Mircea Eugenia AC Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Panaite Tatiana CS 3 Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Ropota Mariana CS 2 Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Partner 1 Bordei Natalita Chem Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Tamas Viorica PhD chem Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Neagu Mihaela Phd biochem Phase 1 Phase 2
2 2 1 2 2 2 2 1 2 2 2 2 1 1 2 2 2 3 3 3 3 2 3 3 3 3 2 2 3 3 43
Phase 3 Phase 4 Phase 5 Cozea Andreea Phd Bioteh Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Traistaru Alina Gina Phd Chem Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Badea Elvira PhD Biol Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Carabela Viorica Eng Chem Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Partner 2 Papuc Camelia Puia PhD. Professor Phase 2 Phase 3 1 1 3 2 2 2 3 3 2 2 2 3 3 2 2 2 3 3 1 2 2 3 3 1 1 2 2 2 1
. Professor Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Crivineanu Maria PhD.
assistant Phase 1 Phase 2 Phase 3
1 1 1 1 1 1 2 4 5 3 1 2 4 5 3 1 2 4 5 3 1 2 4 5 3 1 2 4 5
. lecturer Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Popescu Maria Lidia PhD. lecturer Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Iordache Alina Titina PhD student. assistant Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Nencu Ioana PhD student.Phase 4 Nicorescu Valentin Razvan PhD. lecturer Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Dutu Ligia Elena PhD. Lecturer Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Partner 3 Gird Cerasela Elena PhD.
OIL EXTRACTION INOX. Gas chromatographer for fatty acids determinations PerkinElmer (USA). P1 . OIL
.A.Experimental house. Professor Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Valentina Popa PhD Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Total 261
3 1 2 4 5 3 1 0. Digestibility cages for poultry.. EF-2Bulk ELECTROLAB TESTER ASA Technology. Laboratory mill.C. Electric driers S. FOSS Kjeltec 2300. Deepfreezer ULUF 450. Calcination oven.S. FOSS Soxtec 2055. Pilot station for compound feeds. Whirpool COLD LADA 2003. HARDNESS TESTER Logan HDT-300 ASA Technology. Spectrofluorimeter.Phase 4 Phase 5 Costea Teodora PhD student. HPLC PerkinElmer (USA). Analytical balances Sartorius and Kern. FOSS Fibertec 2010.5 0 2 2 1
Available research infrastructure CO .5 0 2 2 1 0. Nise. Homogenizer. LIQUID.INCDBNA-Balotesti . Egg shell thickness gauge. Ecocell oven 222. Freezer DKF 350. . Egg shell analyzer. Mill Plants. Cardinal Service. grains and seeds with knives and hammers IMUC. Hofigal Export-Import S.C. HPLC Termo Electron. Centrifuge. Rotary-evaporator Buchi. Temperature adjustable oven VENTICELL BMT. assistant Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Partner 4 Andrei Danet PhD. Athomic absorption spectrophotometer.
Chemiluminometer Turner Biosystems (USA). pH-meter. Water bath GFL 1092. Palm Sens (Holland). Laboratory fridges Nitech. Laboratory oven Ecocell. Microcentrifuge Beckman. Supercritical fluid chromatography and extraction system (SFC / SFE) Jasco P4 – UB – Facultatea de Chimie: Spectrophotometers Ocean Optics (USA) with optical fibers. Pneumatic conveying plants TOCATOR Plant. DRY HOT AIR TRAYS-Valcea Ramnicul P2 . Analytical balance.UMF „Carol Davila” – Facultatea de Farmacie: Chromatographic system Reprostar 3. Analytic balance Precisa. System of video-documentation in gel Vilber Lourmat. Binocular microscop with digital photo camera Zeiss. Autoclaves hood with laminary flow. Coling Heating Thermostat Biosan CH 100 P3 . Electric sandbath. Electrochemical equipment for potentiometric. Moisture balance Kern. Electrophoresis device in vertical gel Consort. Rotary evaporator Heidolph. UV lamps. Electric water-bath Raypa. Thermostat Nitech.STORAGE STAINLESS UPRUC. Complete ELISA line. Spectrofotometer UV-VIS V-530 Jasco. SpectrophotometerJena.USAMV – Facultatea de Medicina Veterinara Bucuresti: Ovens Nitech. Electronic balance Shimadzu. Rotary-evaporator Buchi. Vacuum-pump Fischer. Fluorimeter Able Jasco. Chemiluminometer – Berthold FB12. Electrophoresis device in horizontal gel Consort. PH-meter – Sigma. Blender Waring. Spectrophotometer UV-VisNIR V670. Automatic potentiometric titrator Titro Line easy. Turbidimeter Cole Parmer. Centrifuges
. amperometric and voltammetric determinations. Analytic balance KERN.
Budget breakdown by year (lei) Public Budget
Priv ate cof %
(CO) P1 P2 P3 P4 Total
98012 83913 17483 17483 2850 219741
234380 200660 41807 41807 36346 555000
234380 200660 41807 41807 36605 555259
85228 72967 15203 15203 11399 200000
652000 558200 116300 116300 87200
45191 108049 108048 39291 300579
98012 129104 17483 17483 2850 249111
234380 308709 41807 41807 36346 625231
234380 308708 41807 41807 36605 662805
85228 112258 15203 15203 11399 262853
652000 858779 116300 116300 87200 1830579 16.42
Budget breakdown by category of expenses Budget breakdown / destination (lei)1 Personnel costs
Coordinator (CO) Partner 1 Public Budget Private cofinancing Public Budget Private cofinancing Partner 2 Public Budget Private cofinancing Partner 3 Public Budget Private cofinancing
125000 60000 60000
167460 180579 0
100000 36000 25100
558200 300579 116300
According to Chapter 8 – Budget
Memory 4GB. mouse. for 100 mL. video AMD Radeon HD 6750M/512MB. Operating system: MacOS X Lion
Justification .1+EDR. 21. 250 mL.5GHz. Full measurement of antioxidant capacity from plant materials.Complet system for determining antioxidants which are soluble in water and lipids.GDDR5. IntelCore i5. 2.5’’. KB wireless. bluetooth. IR receiver. DDR3 1333Hz.Partner 4
Public Budget Private cofinancing
Justification of purchasing major pieces of equipment Equipment name and characteristics Partner 1 -Computer Desktop all in one. Measurement of whole antioxidant capacity ( the sum parameter) the most diverse and compelx mixtures of plants. Partner 3 -Hot air oven with accesories Flask heaters. 500 mL and 1000 mL flasks with accesories Pharmaceutical sieves with accesories TLC plate heater with accesories Ultrasonic bath with accesories Glass filter holder assembly with accesories
.Proces simulation Tehnical repport edditing
. 2. WI-FI. HDD 500GB/7200rpm.PHOTOCHEM (Analytik Jena Romania srl) Rapid analysis of antioxidants and free radicals with PCL method (Fotochem luminiscent) Complete system for determining antioxidants The first tool that allow a unique determination of both water and lipids soluble antioxidants.Phytochemical analysis
The people want and have the right to know what they eat. generate market benefits for producers of eggs (functional foods). as well as animal welfare. ecology. Impact on the development and promotion of young people – Professional training of young PhD students. 3. there is strong need for specific information and communication activities to consumers in this regard. nutrients. The preconditions for a “good food” are: price. Data that can be used in European databases concerning the food industry by-products that can be used as antioxidants in poultry nutrition. Provide support information for farmers and employers of the feed and food industry. Added value of the project results at National. and management of intellectual property rights The main project result and the dissemination strategy is summarised in the following table:
.5%) and 5 are PhD students. considered waste. through using vegetal material without economic potential. hygiene and taste. Social Impact Because of the limited consumer awareness and limited health effects awareness of new functional ingredients developed. by promoting a healthy lifestyle and with direct implications on the healthcare system.Expected impact 1. decreases the amount of waste stored. Doctoral theses topics are related to study the phytoadditives and the benefits of their inclusion in animal diets (2 PhD students from CO team) and studies on the properties of medicinal plants (3 PhD students from P3 team). Using local food industry by-products as sources of antioxidants.2. Dissemination and/or exploitation of project results. Environmental impact • The project does not prejudice the environmental quality on the contrary.1. having a lower production cost. health. Direct and indirect reduce of costs associated with cardiovascular disease. functional foods thus obtained. Of the 24 key people. master students and students involved in the project. European and International level Economic Impact The project will generate economic benefits to the co-financing economic agent due to the using of a vegetal source with antioxidant potential as an alternative to expensive synthetic products. 9 are young people under 35 years (37. Further consumer’s expectations involve the origin and the image of the product.
wich are supervised by the ProjectCoordinator and the Project Administrator. 34 Month 14. symposium. professional si patronale assossiations)
Information and awareness of consumers
Pending patent application
Month 18. The journal publication or other means of putting foreground in the public domain constitute appropriate alternatives. 30
Member of the network. 29
Feed producers. the nature of the results concerned and the legitimate interests of the participants. The Foreground resulting from the project belongs to that participant which generated it. Contracting Authority Mainstream journals
Obtaining relevant and original results
Communications Month 25. national exhibition
Business field (feed producers. The Background that we will consider represent the relevant information to the project held by the participants prior to their accession to the Consortium Agreement. In order to be able to prove ownership of foreground. 27. 26. 51
Project progress report Articles
Month 3. The process of protection and exploitation of the intellectual property will be regulated by the Consortium Agreement. as well as any intellectual property rights which are needed for carrying out the project or for using foreground. 18. Internet. The beneficiaries will define in the Consortium Agreement what IP is considered necessary with respect to the obligation to grant access rights. poultry industry
Development of a nutritional strategy to optimize feed recipes
An established framework within the Consortium Agreement regulates the Backgroung and the Foregroung intellectual property rights (IPR). 29 Communications Month 29
Participation at conferences. seminar
Business field (feed producers. professional and patronal assossiations
Professional events. 8. professional and patronal assossiations
Strategy Web portal. press release.Result Newsletter
Date Month 2
Target for dissemination National and international research community. all the participants will maintain documents showing the development of the generation of knowledge or results. taking into account the specificity of the project. mailing.
Maintenance. With regard to the quality of the products. complemented with those of other internationally circulating pharmacopoeias. being characterised by the fact that they produce in their own greenhouses and agricultural lands the majority of the raw materials that they use.T. Patent applications and expected use of foreground will be reported in the plan for the use and dissemination of foreground. is a company that is specialised in the manufacture of exclusively natural products. Administrative. Approximately 299 employees work in the company. made in the form of pharmaceutical products. they fulfil the requirements of the European Pharmacopoeia. and I.A. Production. and teas. Legal. cosmetics. Logistics and Distribution. Supply. “Hofigal” is a share-held company comprising an exclusively Romanian private capital. Research and Development. being continually motivated towards a professional and personal perfection. The project is well integrated in the development strategy of company by the specific concerns it has: working feed based on indigenous raw materials rich in nutrients and antioxidants.14000. Production Planning. and teas conform to the international norms regarding the cultivation and harvesting of plants. Quality Control. “Hofigal” S. Regulatory Affairs. Hofigal. UMF. any dissemination will be delayed until a decision about its possible protection has been made (through IPR). these people carry out their activities covering a wide range of fields: Agriculture. Marketing. dietary supplements. as well as the rules for Good Manufacturing Practice. and phytotherapeutic galenical products in Romania. dietary supplements. down to the finished product in the form of pharmaceutical products.18000. regardless of what stage they are in.
. Business case – only for Type 2 projects “Hofigal” is one of the famous brand names when it comes to homeopathic. The company utilises an area of 35 hectares. – all of which represent an integrated system. New Therapies.Each participant will ensure that the foreground it owns is disseminated as swiftly as possible. Research results of the project: a new formulation adequate of variability feed ingredients. Total Quality Management. USAMV. UB. The information in this application is hereby certified to be correct. All of the stages of the process. cosmetics. will be implemented at pilot and industrial scale by cooperation of partners: IBNA. from cultivation (pre-processing. However. and of the quality conditions pertaining to Hofigal philosophy.22000 obtained from German TUV company . These new products will represent an important source of economic development and also will protect the animal food which influence the growth and the health of its. ISO 9001. plant processing). Human Resources. Sales. gemoderivative.
. first name: CRISTE RODICA DIANA Signature: