Stem Cell: Pluripotency Signaling Pathways

Insulin/IGF
P P P P P P

Integrin αβ

FAK Paxillin

FGF PI3K
P P P P P P
Zygote

Epidermal Stem Cells

Wnt LRP
β γ

PIP2 Gqα PLK/GIT CK1 Dvl Caveolin

PIP3 PDK1

Grb2 Sos

Ectoderm

Skin & Hair Progenitor Cells

FZL Rac Fyn
P P

P

Akt

P

Ras Ptc Raf CK1 PKA

Shh
Blastocyst

Neurons Astrocytes Neural Stem Cells Oligodendrocytes

P

FRAT

GSK-3β

APC

Axin β-Catenin

PAK
P

MEK Smo
P

SMAD 2/3 TGFβ
P

SMAD 2/3
P

P

mTOR β-Catenin
P

GSK-3β

ESC

SMAD 2/3

SMAD 4 SMAD 4

p70S6K

Gli ERK1/2
P P

Myoblasts Mesenchymal Adipocytes Fibroblasts Stem Cells Osteochondral Progenitor Cells

Mesoderm
iPS Cells

Cardiac Stem Cells Lymphoid Progenitor Cells Myeloid Progenitor Cells

SMAD 1/5 BMP
P

SMAD 4 SUFU

p53 LIF JAK

Hematopoietic Stem Cells

P

SMAD 1/5

SMAD 1/5 γ-Secretase ECN ICN PEST Nanog p15 p21 Nanog Lefty ADAM ICN Lef/TCF BMI-1 Myc CycD MiR 34a MiR 145 TEAD2
P

YES STAT3 STAT3 P
P

Adult Somatic Cells

Jagged / Delta Presenting Cell

YAP

Lung Progenitor Cells Intestinal Progenitor Cells
Klf-4 Oct-4 Lin28 Sox-2 c-Myc Nanog

Endoderm
Hepatic Progenitor Cells Pancreatic Progenitor Cells

CSL CdkNIA Sox-2 Hes c-Myc Oct-4 Oct-4 Nrarp CycD Nanog Klf-4 Nanog Oct-4 Nanog Klf-4 Oct-4

NUCLEUS

PluriSTEM™ Human ES/iPS Medium
A defined complete medium for the serumfree and feeder-free expansion of human ES/ iPS cells, containing small molecule inhibitors, growth factors, and defined cell culture supplements that enables single cell passaging and maintains high cellular performance with less frequent cell feeding and decreased overall cell culture time. For more information visit: www.millipore.com/stemcells

STEMCCA™
STEMCCA™ lentivirus reprogramming kits make it easier than ever to generate induced pluripotent stem (iPS) cells. STEMCCA™ kits’ use of a single polycistronic lentiviral vector greatly improves efficiency, reproducibility and reduces the number of viral integrations generated during the reprogramming process. Transgenefree iPS cells can easily be generated using our Cre-excisable STEMCCA™ lentivirus along with our cell-permeable TAT-Cre Recombinase fusion protein. For more information visit: www.millipore.com/stemcca

Stem Cell Antibodies and Characterization Kits
EMD Millipore is committed to providing validated stem cell antibodies for common and novel protein targets that are related to all areas of stem cell biology. Our complete line of stem cell antibody products include primary and directly conjugated antibodies for stem cell research and numerous ES cell characterization kits and assays. For our complete line of antibodies and kits visit: www.millipore.com/stemcells

Cytokines and Growth Factors
EMD Millipore offers a comprehensive range of cytokines and growth factors for stem cell cultures including xeno-free, animal-free, and pluripotency-related recombinant proteins. Each produced is thoroughly tested for bioactivity, purity, and endotoxin levels. To see our complete offering visit: www.millipore.com/growthfactors

Selected Small Molecule Modulators of Stem Cell Pluripotency and Differentiation
Inducers of Pluripotency: GSK-3β Inhibitor IX, BIO GSK-3 Inhibitor IV, SB-216763 ID-8 Pluripotin Pyrintegrin Valproic acid Y-27632 Inducers of Differentiation BMP Inhibitor II, DMH1 GSK-3β Inhibitor XII, TWS119 Hepatic Differentiation Inducer, SJA710-6 Neuronal Differentiation Inducer, Isobavachin Stauprimide Trichostatin A

For more information on these and other products visit: www.millipore.com/calbiochem

EMD Millipore is a division of Merck KGaA, Darmstadt, Germany
STEMCCA, PluriSTEM, EMD Millipore and the M logo are trademarks of Merck KGaA, Darmstadt, Germany. Lit. No. PS7223EN00 BS-GEN-13-08088 03/2013 Printed in USA. © 2013 EMD Millipore Corporation, Billerica, MA USA. All rights reserved.

www.emdmillipore.com

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