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Cefadroxil

Molecular formula: C16H17N3O5S Molecular weight: 363.4 CAS Registry No.: 66592-87-8

SAMPLE Matrix: blood Sample preparation: 100 |xL Plasma + 8 mL dichloromethane, shake for 20 min, centrifuge at 2500 rpm for 20 min. Remove 7 mL of the organic layer and evaporate it to dryness under nitrogen or at 60°. Dissolve residue in 200 |xL mobile phase, inject a 20 \iL aliquot. HPLCVARIABLES Column: 150 X 6 Shimpack CLS-ODS (Shimadzu) Mobile phase: MeCN: 0.5 mM phosphoric acid 12:88 Column temperature: 40 Flow rate: 1.5 Injection volume: 20 Detector: UV 254 CHROMATOGRAM Internal standard: cefadroxil OTHER SUBSTANCES Simultaneous: antipyrine KEYWORDS plasma; rat; cefadroxil is IS REFERENCE
Lee, C.K.; Uchida, T.; Kitagawa, K.; Yagi, A.; Kim, N.-S.; Goto, S. Skin permeability of various drugs with different lipophilicity. J.Pharm.Sci., 1994, 83, 562-565

SAMPLE Matrix: blood Sample preparation: 100 |xL Serum + 1 mL MeCN, vortex, centrifuge at 2000 g for 10 min. Remove the aqueous phase and add it to 2.5 mL dichloromethane, vortex, centrifuge, inject a 25 JJIL aliquot of the upper aqueous layer. HPLCVARIABLES Column: 150 x 3.9 5 jxm C18 (Waters) Mobile phase: MeCN: 150 mM ammonium acetate 0.7:99.3, pH 7.0 Flow rate: 1.1 Injection volume: 25 Detector: UV 254 CHROMATOGRAM Internal standard: cefadroxil OTHER SUBSTANCES Extracted: ceftibuten

31.C. Pittman.6 5 ^m Nucleosil SA Mobile phase: 20 mM Ammonium dihydrogen phosphate to final concentration of 20 mM in water:MeOH:MeCN 30:35:35.7 Internal standard: cephradine Limit of quantitation: 1 \xgJmL .A. 38..P. High-pressure liquid chromatographic analysis of BMY-28142 in plasma and urine. Flow rate: 1. 1994. HPLCVARIABLES Guard column: 23 X 4 37-50 |xm Corasil C18 Column: 150 X 4 Nova-Pak Mobile phase: MeCN: 5 mM 1-octanesulfonic acid 12:88 Flow rate: 1 Injection volume: 25 Detector: UV 280 CHROMATOGRAM Retention time: 10 Internal standard: cefadroxil OTHER SUBSTANCES Extracted: cefepime KEYWORDS plasma.KEYWORDS serum. E.Agents Chemother. 1112-1117 SAMPLE Matrix: blood Sample preparation: 500 jxL Plasma + 100 u. centrifuge at 500-600 g at 5° for 10 min.. W. Papp. The pH was adjusted to 3. 55— 59 SAMPLE Matrix: blood Sample preparation: 100 u-L Serum + 10 |xL 200 |xg/mL cephradine in water + 100 |xL 6% trichloroacetic acid. rat..A. cefadroxil is IS REFERENCE Barbhaiya.Agents Chemother.. R.0 with concentrated phosphoric acid.5 mL dichloromethane. S. mouse. Nicolau. K. R. Optimal times above MICs of ceftibuten and cefaclor in experimental intra-abdominal infections. inject 25 |xL supernatant...T. Forgue. HPLCVARIABLES Guard column: Waters Guard-Pak C18 Column: 200 X 4.L 100 |xg/mL cefadroxil + 300 JJLL 5% trichloroacetic acid + 500 |xL MeCN + 1. D. Quintiliani. CO. Shyu. Antimicrob.5 Injection volume: 25 Detector: UV 240 CHROMATOGRAM Retention time: 7. centrifuge at 9000 g for 10 min. 1987.. cefadroxil is IS REFERENCE Onyeji.H. vortex for 10 s.. Antimicrob. CH. vortex. Nightingale. inject a 25 |JLL aliquot of the aqueous supernatant.

Transfer upper layer phase. 413. inject 10100 |JLL upper aqueous phase. K. Silber. cimetidine. ibuprofen.8 buffer 5:95 Flow rate: 1. 1987. shake 5 min. Clin.Chromatogr.M.. Faulkner. 1987. centrifuge 5 min. cefadroxil. inject a 50-80 jxL aliquot. Hiltke. J. cephalexin.. warfarin KEYWORDS serum REFERENCE McAteer.Chem. M. 1788-1790 SAMPLE Matrix: blood Sample preparation: 1 mL Plasma + 1 mL MeCN. centrifuge 5 min.. reconstitute in 100 |xL mobile phase. HPLCVARIABLES Column: 300 X 3.A. propranolol. B. HPLCVARIABLES Guard column: RCSS Silica Guard Pak (Waters) Column: 150 X 4. cephalexin. diazepam. Liquid-chromatographic determination of five orally active cephalosporins. J. cefaclor.KEYWORDS serum REFERENCE Lindgren. 33.D.9 /xBondapak C18 Mobile phase: MeOH: 10 mM pH 4.6 5|xm Ultrasphere Octyl C8 Mobile phase: MeOH: 12. Determination of cefadroxil in serum by high-performance liquid chromatography with cephradine as internal standard.5 mM pH 2. salicylic acid. add 6 mL dichloromethane. vortex for 15 s. phenytoin. R.4 Limit of detection: 150 ng/mL . cephradine Noninterfering: acetaminophen.6 NaH2PO4 (pH adjusted with concentrated phosphoric acid) 20:80 Flow rate: 2 Injection volume: 50-80 Detector: UV 240 CHROMATOGRAM Retention time: 3 Internal standard: cefixime (11) Limit of detection: 1 \±g/ mL OTHER SUBSTANCES Extracted: cefaclor.cefixime.R. Remove the supernatant and evaporate it under a stream of nitrogen. and cephradine-in human serum. 347-350 SAMPLE Matrix: blood Sample preparation: 100 |xL Serum + 10 |xL 5 jx^mL cefixime in MeOH + 100 \xL MeCN. centrifuge at 14000 g for 2 min.. digoxin. shake 30 s.5 Injection volume: 10-100 Detector: UV 240 CHROMATOGRAM Retention time: 6.

Russlie. Fourtillan.. reconstitute with 35 JJLL MeOH: water 5:95. inject a 25 |xL aliquot. SPE. Canafax.B. 200 |xL Plasma or 50 (xL middle ear fluid + 1 mL 50 mM pH 6. Evaporate the eluate to dryness under a stream of nitrogen at 40°. inject an aliquot of the upper aqueous layer (J. vortex. adjusted to pH 6. 109). 10 Suppl B. J.KEYWORDS plasma REFERENCE Brisson.Chromatogr.Chromatogr. equilibrate for 10 min. HPLCVARIABLES Guard column: 10 x 2 5 \xm MOS Hypersil C8 Column: 150 X 2 5 |jim MOS Hypersil C8 Mobile phase: MeCN: 5 mM phosphate buffer containing 5 mM tetrabutylammonium 6: 94.Antimicrob..M. D. 413.35 Injection volume: 25 Detector: UV 210 CHROMATOGRAM Retention time: 12.6 Spherisorb S5-ODS2 C18 Mobile phase: MeOH: 100 mM pH 3 acetate buffer 13:87 Flow rate: 1 Detector: UV 254 . re-equilibrate for 4 min. vortex. inject an aliquot. cefadroxil is IS REFERENCE Yuan.8 phosphate buffer. add to the SPE cartridge. J.8 phosphate buffer.8 mL 500 mg Bond Elut C18 SPE cartridge with 4 mL MeOH and 1 mL 50 mM pH 6. 150 |xL Plasma + 150 |xL MeCN. H.8 phosphate buffer.. dry under vacuum. urine Sample preparation: Plasma.M.B. Transfer supernatant to another tube and add 7 volumes dichloromethane.Chemother. elute with 1 mL MeOH: water 40:60. centrifuge at 1000 g for 10 min. rotate at 20 rpm for 10 min. 11-15 SAMPLE Matrix: blood. Dilute with water.Q. J. centrifuge at 1000 g for 10 min.) Column temperature: 40 Flow rate: 0. wash with 1 mL buffer. 674. A. 1982. Urine. middle ear fluid Sample preparation: Condition a 2. Sensitive assay for measuring amoxicillin in human plasma and middle ear fluid using solid-phase extraction and reversed-phase high-performance liquid chromatography. wash with 1 mL 50 mM pH 6. Z. Pharmacokinetic study of cefadroxil following single and repeated doses. rotate at 20 rpm for 10 min. HPLCVARIABLES Guard column: C18 Column: 150 X 1.30 Internal standard: cefadroxil OTHER SUBSTANCES Extracted: amoxicillin KEYWORDS plasma.. 1987.5 (After 14 min wash column with MeCN: buffer 25:75 for 2 min. 93-99 SAMPLE Matrix: blood. 1995.

barbital. aspirin. Martinez. gentamycin. ceftizoxime. cetazidime. Fluorescamine post-column derivatization for the HPLC determination of cephalosporins in plasma and urine. plasma. 1988. B.. R. ceftriaxone. Peris. theophylline. netilmicin. make up to 50 mL with 100 mM pH 4.6 ng/mL (UV) OTHER SUBSTANCES Noninterfering: amidopyrin. dibekacin.5 m X 0. Mandrou. M.. J. mix.. make up to 50 mL with 100 mM pH 4. diazepam.-E. HPLCVARIABLES Guard column: 10x 47 jim Lichrosorb RP 18 Column: 250 X 4 7 |xm Lichrosorb RP 18 Mobile phase: MeCN: 25 mM pH 7 phosphate buffer 5:95 Flow rate: 1 Injection volume: 10 Detector: F ex 385 em 485 following post-column reaction. The column effluent mixed with 200 |xg/mL fluorescamine in MeCN pumped at 0. Drug Metab. Inject an aliquot of urine directly.. 2993-3010 SAMPLE Matrix: bulk. tetracaine. kanamycin. tobramycin KEYWORDS post-column reaction. 1996. J.5 phosphate buffer 60:40 Flow rate: 1 Injection volume: 10 Detector: UV 254 . 270-272 SAMPLE Matrix: blood. lidocaine.D.3 ng/mL (F).J. H. pharmacokinetics REFERENCE Gimeno..5 phosphate buffer. Take 5 mL of this solution. Torres-Molina. rat. M.25 mL/min and the mixture flowed through a 4. Fabre.. caffeine. F detection may be less susceptible to interferences REFERENCE Blanchine. L. cefmenoxime. add 0.5 phosphate buffer.. UV 260 CHROMATOGRAM Limit of detection: 0.Chromatogr. Influence of probenecid on the renal excretion mechanisms of cefadroxil. inject an aliquot of the supernatant. Granero.Dispos. 24. M.5 mL 30 mg/mL dimethyl phthalate in MeCN: water 1:1. urine Sample preparation: 1 mL Plasma + 1 mL 6% trichloroacetic acid. cefotaxime. 0. centrifuge at 4000 rpm for 10 min. HPLCVARIABLES Column: 300 X 3.9 10 ^m ^Bondapak C18 Mobile phase: MeCN: 10 mM pH 4. 11..25 mm ID coil of PTFE tubing to the detector.Liq. formulations Sample preparation: Homogenize sample.CHROMATOGRAM Limit of detection: 300 ng/mL KEYWORDS plasma. weigh out sample equivalent to 50 mg cefadroxil.

make up to 2 L with water. 1992.1 Flow rate: 1 Injection volume: 20 Detector: UV 254 CHROMATOGRAM Retention time: 5 Limit of quantitation: 1. and 74.0 potassium phosphate buffer.Off. cephalothin. M. J. cefoxitin. Column liquid chromatography and microbiological assay compared for determination of cefadroxil preparations. add to the SPE cartridge. filter (0. Evaporate the eluate to dryness under a stream of nitrogen. remove the supernatant and add it to 20 mL water and 400 (xL tributylamine.0 potassium phosphate buffer 35:65 Flow rate: 1 Injection volume: 200 Detector: UV 210. Y-W. 71. cephradine REFERENCE Ting.4 g EDTA. heat at 60° for 10 min..Chromatogr. 181-186 SAMPLE Matrix: bulk. centrifuge for 10 min. Charm II assay . inject an aliquot of the filtrate. (Buffer was 545 mL 100 mM citric acid. 455 mL 200 mM Na2HPO4.5 with ammonium hydroxide.Chem. inject a 20 (JLL aliquot. S. wash with two 2. cefoperazone.Anal.2 ixm).9 10 |xm ixBondapak C18 Mobile phase: MeOH: water: acetic acid 30:70:0. cefamandole nafate.CHROMATOGRAM Retention time: 2 Internal standard: dimethyl phthalate Limit of quantitation: 20 [xg/mL KEYWORDS capsules.Assoc. formulations Sample preparation: Dissolve in water to a concentration of 40 |xg/mL. cefazolin. ceftizoxime. Chang. 1123-1130 SAMPLE Matrix: milk Sample preparation: Condition a Bond Elut C8 SPE cartridge with 5 mL MeOH and 5 mL water. HPLCVARIABLES Column: 300 X 3..5 mL portions of water. cephapirin. adjust to pH 4. shake well..-C.6 10 |xm Lichrosorb RP-8 Mobile phase: MeOH: 50 mM pH 6. elute with 2. cefaclor.5 mL MeOH. cefamandole. J. extract the residue with three 100 |xL portions of 50 mM pH 6. Lee. 20 mL Milk + 1 mL 1 M oxalic acid. Reverse-phase liquid chromatographic analysis of cephalosporins. 609. cefotaxime. cephalexin.8 jxg/mL OTHER SUBSTANCES Simultaneous: impurities. powders REFERENCE Hsu.) HPLCVARIABLES Column: 250 X 4.-T. Y. 1988.

cephapirin. V.5. LC-Receptograms: A method for identification and quantitation of (J-lactams in milk by liquid chromatography with microbial receptor assay. Cefadroxil as model drug and sodium lauryl sulfate as model surfactant: Studies in rat colon.9 4 |xm Novapak C18 (Teknochroma) Mobile phase: MeCN:buffer 16:84.W. Flow rate: 1 Detector: UV 254 CHROMATOGRAM Retention time: 6.9 with dilute NaOH (Buffer was 5..456 g sodium laurylsulfate in 1 L water. cefotaxime. cefazolin. ticarcillin Simultaneous: ampicillin. 78. Bengochea. Fabra-Campos. D-hydroxyphenylglycine REFERENCE Slingsby.AOAC Int.. dicloxacillin.. S. J. Determination of Pharmaceuticals by multi-phase chromatography: Combined reversed phase and ion exchange in one column. Charm. Experimental studies on the influence of surfactants on intestinal absorption of drugs. 107-134 . cephalothin. J. cephalexin.Chromatogr. M. ceftiofur. cephalosporin C. Gomez-Meseguer.8 mL glacial acetic acid and 2. B was MeCN: 300 mM perchloric acid 45:55. R... A. oxacillin. Arzneimittelforschung.OTHER SUBSTANCES Extracted: amoxicillin.5:86. cloxacillin. A:B from 100:0 to 0:100 over 7 min. 1165-1172 SAMPLE Matrix: perfusate HPLCVARIABLES Guard column: 40 |xm C18 (Teknochroma) Column: 150 X 3. S. V. J. 13. Saul. nafcillin. 595-601 SAMPLE Matrix: solutions HPLCVARIABLES Column: 250 X 4 OmniPac PCX-500 (Dionex) Mobile phase: Gradient.E. penicillin G KEYWORDS SPE REFERENCE Zomer. Quintana. maintain at 0:100. 45. adjusted to pH 3. E.Liq. 1990.. S. M.) Flow rate: 1 Detector: UV 280 KEYWORDS rat REFERENCE Sancho-Chust.. A was MeCN: 90 mM perchloric acid 13. 1995.. Martin-Villodre.. 1995. Rey. cephapirin.. cephaloridine.5 OTHER SUBSTANCES Simultaneous: 7-aminocephalosproranic acid.

adjust pH to 3.J. R. Cephalosporin penetration into soft tissue of paralyzed limbs. J. HPLCVARIABLES Column: 150 X 4. C. Plasz. HPLCVARIABLES Column: 4 jxm Novapak C18 Mobile phase: MeCN: 5 mm sodium heptanesulfonic acid 9:91.. White. C. R. 33.6 Limit of quantitation: 100 ng/mL REFERENCE Gorski. muscle. centrifuge at 1600 g for 5 min. L.... A. remove excess liquid with a second swab. inject a 50 |xL aliquot..Pharm. Ou. Musher. Rognerud. inject a 50 |xL aliquot. 125 |xL Supernatant + 100 JJLL water + 800 JJLL MeCN. cefadroxil is IS REFERENCE Darouiche. L. Shaohong. 1525—1527 SAMPLE Matrix: tissue Sample preparation: Homogenize muscle with three volumes phosphate-buffered saline (Polytron..7 Internal standard: cefadroxil OTHER SUBSTANCES Extracted: cefepime KEYWORDS mouse. J. The chromatographic behavior of cephalosporins in gel filtration chromatography. Antimicrob. Yoder. S.. Kaimin. 8. Elrod.J. cefmenoxime. filter (0. .6 5 |xm Nucleosil C18 Mobile phase: MeCN: 70 mM KH2PO4 4:96 Flow rate: 1 Injection volume: 50 Detector: UV 254 CHROMATOGRAM Retention time: 5. Determination of cefsulodin. reconstitute the residue in 125 \±L mobile phase.Res.45 jxm polycarbonate).. level 3) for 2 min. and cefadroxil as residues on surfaces..B. 1326-1328 • ANNOTATED BIBLIOGRAPHY • • Changqin... centrifuge at 1300 g for 10 min. D. W. a novel method to separate high molecular weight impurities. 1989. R. Remove the supernatant and evaporate it to dryness under a stream of nitrogen. H.SAMPLE Matrix: surface wipes Sample preparation: Swab 100 X 100 mm surface with water (total volume 10 mL).33 with glacial acetic acid Flow rate: 2 Injection volume: 50 Detector: UV 280 CHROMATOGRAM Retention time: 6.Agents Chemother. 1991.C. Hamill. vortex swabs for 45 s. vortex for 30 s. Pharm.

Steijer. 61-70 [extracted amoxicillin. Masuda. Van de Merbel.A. cephalexin... J. Pharm.. cefazolin. cephradine] Muranushi. cephalexin. Automated column liquid chromatographic determination of amoxicillin and cefadroxil in bovine serum and muscle tissue using on-line dialysis for sample preparation. muscle. N. 533-541 [also cefamandole. ceftriaxone. Liquid chromatographic method for the determination of cefadroxil in its suspension and in serum. Jackson. latamoxef] Snippe.. J. serum. cow. O. N. H. Characteristics of ceftibuten uptake into Caco-2 cells. Brinkman.Liq.M. 1761-1765 [also cefaclor..Res. cephradine. 1994. LOD 50 ng/mL. K.C.T. cephaloridine. Ruiter. N. Lingeman.S. ceftazidime. cefotaxime.. U. 1994. 11. RRM. cefoperazone. LOD 200 ng/g] Nahata. cephalothin. Hirano.Chromatogr. 1990. SPE. K. Horie.AnaL. 662.Biomed.Chromatogr. 1651-1656 [suspensions. cefmenoxime. on-line dialysis. cyclacillin. 12.C. post-column reaction.. cefaclor (IS)] .. serum. D..B. 1994. M. 13.. K. ceftibuten.