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Cefixime

Molecular formula: C16H15N5O7S2 Molecular weight: 453.4 CAS Registry No.: 79350-37-1

SAMPLE Matrix: bile, blood Sample preparation: Plasma. 25 |xL Plasma + 25 |xL buffer + 450 |xL 5% trichloroacetic acid, mix, centrifuge, inject a 10 |xL aliquot of the supernatant. Bile. 100 |xL Bile + 900 J L J L L buffer, mix, centrifuge at 2000 rpm for 5 min, inject a 10 J U L L aliquot of the supernatant. (Buffer was 2.54% NaH2PO4.2H2O and 4.41% Na2HPO4.12H2O, pH 7.4.) HPLCVARIABLES Column: 150 X 4.6 Chemcosorb 7C18 (Chemco) Mobile phase: MeCN: buffer 11:89 (plasma) or 13:87 (bile) (Buffer was 20 mM (NH4)H2PO4 adjusted to pH 3.2 with phosphoric acid.) Column temperature: 40 Flow rate: 2 (plasma), 1 (bile) Injection volume: 10 Detector: UV 290 KEYWORDS rat; plasma REFERENCE
Yasui, H.; Yamaoka, K.; Nakagawa, T. Alternative continuous infusion method for analysis of enterohepatic circulation and biliary excretion of cefixime in the rat. J.Pharm.ScL, 1994, 83, 819-823

SAMPLE Matrix: blood Sample preparation: 100 |xL Serum + 10 jxL 5 |xg/mL cephalexin in MeOH + 100 |xL MeCN, vortex for 15 s, centrifuge at 14000 g for 2 min. Remove the supernatant and evaporate it under a stream of nitrogen, reconstitute in 100 |xL mobile phase, inject a 5080 |xL aliquot. HPLCVARIABLES Guard column: RCSS Silica Guard Pak (Waters) Column: 150 X 4.6 5|xm Ultrasphere Octyl C8 Mobile phase: MeOH: 12.5 mM pH 2.6 NaH2PO4 (pH adjusted with concentrated phosphoric acid) 20:80 Flow rate: 2 Injection volume: 50-80 Detector: UV 240 CHROMATOGRAM Retention time: 11 Internal standard: cephalexin (15) Limit of detection: 100 ng/mL OTHER SUBSTANCES Extracted: cefaclor, cefadroxil, cephradine

inject a 100 |xL aliquot. inject 75 |xL supernatant. and cephradine—in human serum. Reeves. (Buffer was 66. inject a 20 |JLL aliquot of supernatant. Liquid-chromatographic determination of five orally active cephalosporins—cefixime. Pleural.Chem. D. Cut tissue with a scalpel. centrifuge.A. Cut tissue with a scalpel.P.D. propranolol. A. 1987. inject a 20 |xL aliquot of supernatant. centrifuge at 9600 g for 5 min three times.. inject a 20 |xL aliquot. Tissue (lung. CSF Sample preparation: 150 |xL Serum or CSF + 150 |xL 6% trichloroacetic acid.36 g NaH2PO4 + 2 mL phosphoric acid + 828 mL water adjusted to pH 2. HPLC assay of cefixime in serum and CSF. adjusted to pH 5. L. Urine. warfarin KEYWORDS serum REFERENCE McAteer. method has advantages over that of Falkowski et al. A. B.4 mM K2HPO4 adjusted to pH 5. Silber. phenytoin. 422. centrifuge.R. diazepam. cimetidine. 1788-1790 SAMPLE Matrix: blood. homogenize with 3-6 mL buffer in an ice bath for 2-3 min.6 3 |xm Techsphere C18 Mobile phase: 170 mL MeCN + 1. 31. tissue.) HPLCVARIABLES Column: 200 X 4 5 |xm Nucleosil C18 Mobile phase: MeOH: buffer 15:85. Dilute urine 1:10 to 1:100 with buffer. homogenize with 1-3 mL buffer. 33. 1987. inject a 20 |xL aliquot. Lovering. Clin.. 1993. centrifuge.O.Chemother. J. M. Dilute serum or plasma 1:2 to 1:10 with buffer. cefaclor..M. Tissue (chondral). ibuprofen.6 mM K2HPO4 adjusted to pH 7. cefadroxil. MacGowan. J. Dilute human pleural samples with buffer.M.40 with KH2PO4. cephalexin. urine Sample preparation: Serum. Hiltke. centrifuge for 3 min.Noninterfering: acetaminophen. R..2 with phosphoric acid (Buffer was 57. gut)..Antimicrob. Chromatogr.) Flow rate: 1 Injection volume: 20-100 Detector: UV 230 CHROMATOGRAM Retention time: 9 Limit of detection: 100 ng/mL .7 Flow rate: 2 Injection volume: 75 Detector: UV 313 KEYWORDS serum. centrifuge at 9600 g for 5 min four or five times. (J..2 with phosphoric acid. 145152) REFERENCE White.S.. Faulkner. 450-451 SAMPLE Matrix: blood. digoxin. HPLCVARIABLES Column: 150 X 4. plasma. salicylic acid.

36 g/L KH2PO4. J. Bremm.. urine Sample preparation: Serum. Look.7 (urine) Flow rate: 2 Injection volume: 75 Detector: UV 280 (serum).5 (serum).2 containing 1. 6.Chromatogr.8:0. centrifuge at 9300 g for 4 min..D. centrifuge at 30000 g for 2 min.40 with 50 mmol KH2PO4 (Soerensen buffer). (Buffer was 60 mmol K2HPO4 adjusted to pH 7. W. vortex at high speed for 15 s.. Determination of cefixime in biological samples by reversed. rat.25 |xg/mL IS in 6% trichloroacetic acid in water. 1988. 427. B. UV 313 (urine) CHROMATOGRAM Retention time: 3. urine Sample preparation: Dilute serum 1:10 with buffer. Z. A. Silber. caffeine. inject 20 jxL of supernatant.7 (serum) or MeCN: water: 85% phosphoric acid 20:79. centrifuge at 30000 g for 2 min.. M.6 (serum). theophylline KEYWORDS serum. W.2 containing 1. inject a 75 |xL aliquot of the supernatant. gut. monkey REFERENCE Falkowski. dog. 250 JULL Serum + 250 |xL 4 |xg/inL IS in 6% trichloroacetic acid in water.. lung. Application of high-performance liquid chromatography of some antibiotics in clinical microbiology. human. 100 |xL Urine + 2 mL 0.water:85% phosphoric acid 17:82. Schonfeld.Chromatogr. 145-152 SAMPLE Matrix: blood..20 with phosphoric acid Flow rate: 1 Injection volume: 20 Detector: UV 230 CHROMATOGRAM Retention time: 12 Limit of detection: 100 ng/mL .36 g/L KH2PO4.. 257-267 SAMPLE Matrix: blood. 4 (urine) Internal standard: 7-hydroxycoumarin (5. K. H.8 (urine)) Limit of quantitation: 50 ng/mL (serum). pleural.8:0.M. salicylic acid. Noguchi. vortex at high speed for 15 s. dilute urine 1:10-100 with buffer. J. 1987.. chondral REFERENCE Knoller..KEYWORDS serum. 422. Urine. pH 2. inject a 75 |xL aliquot of the supernatant. Roller.) HPLCVARIABLES Column: 200 X 4 Nucleosil 5 C18 Mobile phase: MeOH: 43 mM K2HPO4 15:85 adjusted to pH 5. pH 2.phase high-performance liquid chromatography. 1000 ng/mL (urine) OTHER SUBSTANCES Noninterfering: acetaminophen.M. J. plasma. HPLCVARIABLES Column: 100 X 8 5 |xm Nova-Pak C18 RCM-100 Mobile phase: MeCN. Kdnig.J.

L. CSF.D. J. S.. Gao.X.G.Liq. Shen. LOD 30 ng/mL] . G.. Schonfeld.... 1991. Wang.Chromatogr. Sha. J. J. Konig. M. W. 1993. 1987. [Determination of cefixime in human plasma and urine using high performance liquid chromatography column switching technique].. Bremm. 7-hydroxycoumarin (IS). K. 3755-3759 [serum.. urine and buffer. S.C. Y. 14. R. 389.Chromatogr. Yao Hsueh Hsueh Poo. Measurement of cefixime in serum and cerebrospinal fluid by high-performance liquid chromatography. In vitro stability of cefixime (FK-027) in serum.. 312-316 • ANNOTATED BIBLIOGRAPHY • • Liu. W. 28.KEYWORDS serum REFERENCE Knoller.X. 216-221 Nahata..