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Biology 383 Stream Ecology Analysis of Biological Samples

Introduction: In today s lab we will process the biological samples from earlier labs. The samples were taken to capture benthic invertebrates and fish. The processing of samples includes sorting out the organisms into like groups and using a taxonomic key to identify them. You will use these data to describe the stream section we have been studying over the last few weeks. Benthic Invertebrates: Work with one sample at a time and proceed carefully. Sort the sample by picking out aquatic organisms and separating them from the rest of the sample. Use the stereo microscopes to make sure you have found all of the organisms. One separated, identify the organisms using the keys provided and reference texts in the laboratory. Identify organisms to the level of Family at least. Record the information from each sample on the data sheet provided. Calculate the Biotic Indices indicated. Fish: Fish population analysis involves the estimation of total number of each species from the removal sampling method employed. Data are analysed with the help of a computer program known as Microfish 3.0. Results from this analysis of last week s work will be available in lab - make sure you get a copy!

Lab Report: Write up the work from the last 2 labs and this one (4, 5 & 6) into a scientific report entitled, The Biota of Southwest Brook. This will be due in 2 weeks - November 17, 2003.

Lab Six

Biology 383

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Data Sheet for Benthic Macroinvertebrates


Date Sample Collected: ______________________Site:_____________________________ B B No. of Organisms ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ X C C Biotic Score* ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ = D D Total ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________

A Family 1. ____________ 2. ____________ 3. ____________ 4. ____________ 5. ____________ 6. ____________ 7. ____________ 8. ____________ 9. ____________ 10.____________ 11.____________ 12.____________ 13.____________ 14.____________ 15.____________

Family Biotic Index = Total of Column D divided by Total of Column B = ________________ % EPT = Total Ephemeroptera, Trichoptera, and Plecoptera divided by Total of Column B =____________ Taxa Richness = Total No. of taxa = _________________ Biotic Score - See Xerox of Appendix 31.1 Resh et al 1996 Lab Six Biology 383 Page: 2

Compare your FBI with the following table.

Water Quality Based on Family Biotic Index Values


From Hilsenhoff (1988) Family Biotic Index 0.00 - 3.75 3.76 - 4.25 4.26 - 5.00 5.01 - 5.75 5.76 - 6.50 6.51 - 7.25 7.26 - 10.00 Water Quality Excellent Very Good Good Fair Fairly Poor Poor Very Poor

References: Hilsenhoff, W.L. 1988. Rapid field assessment of organic pollution with a family level biotic index. Journal of the North American Benthological Society 7: 65-68 Resh, V.H., M.J. Myers, and M.J. Hannaford. 1996. Macroinvertebrates as Biotic Indicators of Environmental Quality in Hauer, F.R. and G.A. Lamberti (eds.) Methods in Stream Ecology. Academic Press, New York. 674 pp.

Lab Six

Biology 383

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