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of fabricius destruction.
Birna -bi +rna -double stranded RNA virus.
o o o o
Family Genus Species
: birnaviridae : avibirna virus :infectious bursal disease virus
Acronym : IBDV Virus morphology: -virions non enveloped -bisegmented genome -hexagonal icosahedral symmetry.
- 1 and 2 • Serotype 1 -young chickens • Serotype 2 -no clinical signs
Antigenic property: - No HA
1. Resistant to : Acid pH 3 to 7 Alkali pH 7 to 9 2. Virions not sensitive to treatment with heat (60 C, one hour) or ether. 3. Sensitive to pH below 3 & above 9.
CULTIVATION: Chickens , ECF, CEF.
1. CHICKENS: -not recommended. -via eyedrop. -kill chicken 72 to 82 hrs after inoculation. 2. Embryonated chicken eggs: -route of inoculation (i) Yolk sac -5 to 7 days (ii) Chorioallantoic membrane – 12 days.
specific lesions • Dwarfing of embryo • Kidney, liver ,spleen- enlarged. • Congestion and subcutaneous oedema. 3.cell culture: • Chicken embryo fibroblast used.
• -infection oral route. • spread by fomites. • carrier state vertical transmiision not demonstrated.
PATHOGENESIS Host: Chicken, turkey and ducks Route of transmission: Water, feed , infected birds droppings Carrier : Meal worm and Aedes vexan (Mosquito) Morbidity – 100 % Mortality - broilers 25% - layers 60% Incubation period : 2-3 days. Ingestion ↓ macrophages and lymphoid cells in Caeca
Duodenum Jejunum ↓ liver via portal circulation ↓ bursa of fabricius ↓ Replication in cytoplasm ↓ Target cell – B lymphocytes ↓ Destroys lymphoid follicles in the bursa of fabricius
SYMPTOMS • Self vent pecking • Anorexia • Depression • Trembling • Watery and whitish diarrhea • Prostration • death. • Recovered birds – atrophied bursa of fabricius LESIONS 1.Bursa of fabricius a.swollen & haemorrhage b.atrophy. 2.Dehydrated carcass
3.haemorrhagic dark skeletal muscles 4.Swollen liver and kidneys 5.Intestines with increased mucus
DIAGNOSIS: 1. Clinical symptoms 2. lesions.
Isolation and identification: a. Clinical materials: 1. Fresh bursa and spleen 2. HP-Samples of bursa, spleen, intestines, liver and kidney in neutral buffered formalin. 3. Serology-Blood samples
3.Serological tests 1. AGID 2. Quantitative AGID test. 3. Virus neutralization test 4. ELISA 5.FAT
4.serotype differentiation - employing specific monoclonal antibodies • Cross neutralization test. • Virus neutralization test. • Serological test .
DIFFERENTIAL DIAGNOSIS • Marek’s diseases, • Mycotoxicosis, • coccidiosis, • Haemorrhagic syndrome, • Avian adenovirus infection • Infectious bronchitis.
TREATMENT: Not effective
IMMUNITY 1. VACCINES: IBD vaccines prepared serotype 1 IBDV only.
a. Live vaccines: Only healthy birds should be vaccinated. 1.Mild –fully attenuated(lost its virulence but retained immunogenicity) 2. Intermediate – partially 3.Intermediate plus -not attenuated
-8 wks - Intramuscular injection - Spray –aerosal - Drinking water directly added.
b.Inactivated vaccines: 1.breeder hens – only healthy birds vaccinated. 2.produce high, 3.long lasting . 4.uniform levels of antibodies.
ERADICATION: • Recovered and vaccinated shed virus for long periods. Hence disease control
• depopulation • rigorous disinfection of contaminated premises. • Disposal of litter, dead birds, used gunny bags, curtains by incineration or deep burial with slaked lime. ZOONOTIC IMPORTANCE: Not report