You are on page 1of 84

Gebrauchsanleitung Operating Instructions Mode d'emploi Manual de instrucciones

Titrator TitroLine alpha plus Titration Unit TitroLine alpha plus Titrateur TitroLine alpha plus Titulador TitroLine alpha plus

Gebrauchsanleitung ........................................................................................................... Seite 1 ....... 82
Wichtige Hinweise: Die Gebrauchsanleitung vor der ersten Inbetriebnahme des Titrators TitroLine alpha plus bitte sorgfältig lesen und beachten. Aus Sicherheitsgründen darf der Titrator TitroLine alpha plus ausschließlich nur für die in dieser Gebrauchsanleitung beschriebenen Zwecke eingesetzt werden. Bitte beachten Sie auch die Gebrauchsanleitungen für die anzuschließenden Geräte. Alle in dieser Gebrauchsanleitung enthaltenen Angaben sind zum Zeitpunkt der Drucklegung gültige Daten. Es können jedoch von SCHOTT sowohl aus technischen und kaufmännischen Gründen, als auch aus der Notwendigkeit heraus, gesetzliche Bestimmungen der verschiedenen Länder zu berücksichtigen, Ergänzungen am Titrator TitroLine alpha plus vorgenommen werden, ohne dass die beschriebenen Eigenschaften beeinflußt werden.

Operating Instructions .................................................................................................... Page 83 .... 164
Important notes: Before initial operation of the Titration Unit TitroLine alpha plus please read and observe carefully the operating instructions. For safety reasons the Titration Unit TitroLine alpha plus may only be used for the purposes described in these present operating instructions. Please also observe the operating instructions for the units to be connected. All specifications in this instruction manual are guidance values which are valid at the time of printing. However, for technical or commercial reasons or in the necessity to comply with the statuary stipulations of various countries, SCHOTT may perform additions to the Titration Unit TitroLine alpha plus without changing the described properties.

Mode d’emploi ................................................................................................................ Page 165 .... 246
Instructions importantes: Prière de lire et d’observer attentivement le mode d'emploi avant la première mise en marche du Titrateur TitroLine alpha plus. Pour des raisons de sécurité, le Titrateur TitroLine alpha plus pourra être utilisé exclusivement pour les usages décrits dans ce présent mode d'emploi. Nous vous prions de respecter également les modes d'emploi pour les appareils à connecter. Toutes les indications comprises dans ce mode d’emploi sont données à titre indicatif au moment de l'impression. Pour des raisons techniques et/ou commerciales ainsi qu'en raison des dispositions légales existantes dans les différents pays, SCHOTT se réserve le droit d'effectuer des suppléments concernant le Titrateur TitroLine alpha plus pour séries de dilution qui n’influencent pas les caractéristiques décrits.

Manual de instrucciones ............................................................................................ Página 247.. 325
Instrucciones importantes: Primeramente, lean y observen atentamente el manual de instrucciones antes de la primera puesta en marcha del Titulador TitroLine alpha plus. Por razones de seguridad, el Titulador TitroLine alpha plus sólo debe ser empleado para los objetivos descritos en este manual de instrucciones. Por favor, respeten las indicaciones descritas en los manuales de instrucciones de los equipos antes de conectarlos. Todos los datos contenidos en este manual de instrucciones son datos orientativos que están en vigor en el momento de la impresión. Por motivos técnicos y/o comerciales, así como por la necesidad de respetar normas legales existentes en los diferentes países, SCHOTT puede efectuar modificaciones concernientes al Titulador TitroLine alpha plus sin cambiar las características descritas.

83
TABLE OF CONTENTS

TABLE OF CONTENTS PAGE

1.. Technical properties of the TitroLine alpha plus Titration Unit ..........................85
1.1 1.2 Summary ......................................................................................................................................85 Warning and safety information ...................................................................................................91

2.. Set-up and first operation ......................................................................................92
2.1 2.1.1 2.1.2 2.1.3 2.2 2.3 2.4 2.5 2.6 2.7 Unwrapping and set-up of the TitroLine alpha plus Titration Unit...............................................92 Installation of the stand rod ......................................................................................................92 Connection of the optional TM 135 titration rig. .......................................................................92 Installation of the titration clip ...................................................................................................92 Installation of the TM KF Titration Stand and of the titration vessel ...........................................93 TA plus Interchangeable Unit:......................................................................................................96 Fitting and replacing an interchangeable unit ..............................................................................97 Replacing the glass cylinder ........................................................................................................98 Initial filling and rinsing of the complete interchangeable unit .....................................................99 Combining the TitroLine alpha plus Titration Unit with other devices........................................100

For a functional description, please refer to chapter 5, “Working with the TitroLine alpha plus Titration Unit”. In addition, please note the information provided with the devices being connected to the TitroLine alpha plus Titration Unit. ....................................................................................................100

3.. Construction of the TitroLine alpha plus Titration Unit.....................................101 4.. The connections of the TitroLine alpha plus Titration Unit ..............................102
4.1 4.2 Measurement-amplifier, A input channel , electrode signal.......................................................102 Measurement-amplifier, B input channel , electrode signal.......................................................102

5.. Working with the titration unit .............................................................................103
5.1 Functioning of the keyboard.......................................................................................................103 Multifunctional graphics display: ...........................................................................................................103 Contrast setting: ....................................................................................................................................103 5.2 The start menu ...........................................................................................................................105 5.3 The mode menu .........................................................................................................................105 5.3.1 Changing a method ..........................................................................................................106 5.3.2 Calculation menu item ............................................................................................................127 5.3.3 TL alpha plus formulae editor.................................................................................................132 5.3.4 Documentation menu item .....................................................................................................138 5.3.5 Method management..............................................................................................................139 5.3.6 Create new method ................................................................................................................140 5.3.7 System settings ......................................................................................................................141 5.3.8 Measurement..........................................................................................................................150 5.3.9 Output .....................................................................................................................................151 5.4 The Cal menu.............................................................................................................................152

6.. Method creation ....................................................................................................154
6.1 154

7.. In/Output for data communication ......................................................................155
7.1 7.2 7.3 7.4 7.5 7.6 Keyboard port.............................................................................................................................156 Power connector ........................................................................................................................156 Centronics-Interface...................................................................................................................156 RS-232-C-Interfaces ..................................................................................................................157 Loading the operating-system software via the RS-232-C interface .........................................157 Controlling the TitroLine alpha plus Titration Unit via the RS-232-C interface .........................158

8.. Maintenance and care of the TitroLine alpha plus Titration Unit .............................159
8.1 Maintenance and care of the titrator section..............................................................................159

84
8.2

TABLE OF CONTENTS Maintenance and care of the burette section.............................................................................159

9.. Storage and transportation ..................................................................................160 10Recycling and disposal ........................................................................................160 11Reference list ........................................................................................................161
Vers: 040714-E

Fig. 1

fotofinishing. µA. e. and curve printouts using a printer with a parallel interface. Owing to its connections the TitroLine alpha plus Titration Unit is very versatile to use. . The possibilities include pH. These methods are just examples. and acid number in polymers − Redox titrations such as iodometry. TBN. p and m value − Acid/base determination in non-aqueous solutions. and process monitoring. environment.1 Technical properties of the TitroLine alpha plus Titration Unit Summary The design of the TitroLine alpha plus Titration Unit is compact and very solid. for use in biochemistry or on soil samples − indices such as OH number. or for dead-stop titrations. and pH-stat titrations with up to 50 methods which can be stored in memory. The Centronics interface is used for the documentation of results. chromatometry. It may also be used for connecting an analytical weighingbalance. e. up to highly complex and difficult. As a matter of course. − − − − − − − − One of the two RS-232-C interfaces (Computer) is used for extensive data communication with a computer (Personal Computer) The second RS-232-C interface enables up to 16 SCHOTT-Instruments devices to be operated within one daisy chain: TITRONIC® universal. or saponification numbers. and CSB determinations − Other mV titrations. fluoride. e. e. calcium. The other 2-channel jack socket can be used to connect a TM 125 magnetic stirrer and to control the TM KF titration rig (on/off).g. The stirring speed can be set on the stirrer/titration rig assembly. The standardized socket for a standard PC keyboard (German keyboard layout) enables simple parameterisation for many functions. iodine number. manganometry. copper. Karl Fischer. One of the two 2-channel jack sockets can be used to operate a TM 125 magnetic stirrer or a TM 128 stirrer plus the TM KF titration rig.g. Its potential stretches from simple end-point titrations (EP) such as the determination of the total acid in wine. biochemistry.g. lead ions − Water determinations according to Karl Fischer. a lot of further applications can be found in food technology.g. data. TAN. pharmaceutics. halogenides next to each other − Titrations using ion-sensitive electrodes. chloride. the automatic TitroLine alpha plus Titration Unit is also the ideal tool for pH-stat applications such as the determination of the enzyme activity.85 Chapter 1 Technical properties 85 1 1. The illuminated graphics LCD display with its corresponding dialogue menus and status displays enable safe operation of the TitroLine alpha plus Titration Unit. such as the water determination according to Karl Fischer (KF). non-aqueous titrations such as the determination of the total acid and base number in oils (TAN/TBN). TITRONIC® T110 plus. and the device is highly versatile and variable to use. Examples of the possible applications of the TitroLine alpha plus Titration Unit include: − Acid/base determination in aqueous solutions. TITRONIC® 110/T 200 and TW alpha/TW alpha plus sample changer. bromine number in oils − pH-stat titrations. quality control.

2003 SCHOTT Instruments GmbH Hattenbergstraße 10 55122 Mainz Deutschland. Allemagne 05. Germany.12. dass das Produkt We declare under our sole responsibility that the product Nous déclarons sous notre seule responsabilité que le produit Titrator Titration Unit Titrateur TitroLine alpha plus auf das sich diese Erklärung bezieht. Teil 3 und mit dem normativen Dokument and the normative document et au document normatif Technische Daten 17. übereinstimmt mit den Normen TitroLine alpha plus to which this declaration relates is in conformity with the standards TitroLine alpha plus auquel se réfère cette déclaration est conforme aux normes DIN EN ISO 8655. 5 Août 2002 AGQSF 0000-A070-00/020805 st .KONFORMITÄTSERKLÄRUNG DECLARATION OF CONFORMITY DÉCLARATION DE CONFORMITÉ Wir erklären in alleiniger Verantwortung. August. August 5 .

00 . 0 ..0 . When working with inflammable substances.001 0. the explosion-protection regulations of the employers' liability insurance association of the chemical industry have to be observed..1. SCHOTT Instruments GmbH Hattenbergstraße 10 55122 Mainz Deutschland.0 .. 2000 . Reference electrode 1 x 4 mm socket Measurement range Over-range Display reso.Measurement Input resisA channel lution accuracy* without tance sensor probe [Ω] pH A PH 0.001 0. Part 1 Country of origin: Made in Germany The following solvents / titration reagents may be used: − All common titration solutions − Water and all non-aggressive inorganic and organic liquids can be used as solvents.2003 DIN EN ISO 8655.. 1800 Over-range . Liquids with a viscosity above 20 mm²/s cannot be dosed. 1820 Display reso. Termination: Electrode socket according to DIN 19 262.Translation of the legally binding german version Technical data of the TitroLine alpha plus Titration Unit Conformity: CE-Sign: Release 17.. 14. 14. as amended by the Directive 93/68/EEC of the Council Testing basis: EN 61 010.1.02 ± 1 Digit > 1 • 1013 mV A . 4 smoothening levels of the measurement signal are possible in the A channel.. a socket insert (Z 860) may be used. 0 ..02 ± 1 Digit > 1 • 1013 0. Part 1 Jamming resistance according to the EN 61 326 standard.2000 .00 0. the connector of the temperature sensor probe must NOT be connected to the common earth in the case of single-rod electrodes with an integrated temperature probe. − With liquids with a higher viscosity (≥ 5 mm²/s). 1920 0..0 ± 1 Digit > 1 • 1013 B channel pH B mV B PH U [mV] Measurement range 0.. 5 Août 2002 AGQSF 0000-A070-00/020805 st .1820 .1 1..0 ± 1 Digit > 1 • 1013 To ensure maximum accuracy of the readings we recommend to allow some reasonable time for the TitroLine alpha plus Titrator to “warm up”. with the BNC version..1 U [mV] 1. Measuring input: A and B channel. August 5 . Allemagne 05. a low boiling point or a tendency to exhalation the filling and dosing speed may be adapted.Compatibility according to the directive 89/336/EEC of the Council. Please note with regard to the B measurement channel: To maintain the galvanic insulation from the A channel.Measurement Input resislution accuracy* without tance sensor probe [Ω] 0. In this case a separate PT 1000 temperature probe has to be used for temperature measurement or temperature compensation. Part 2 Low-voltage directive according to the Directive 73/23/EEC pf the Council..00 .2020 . galvanic insulation of the B channel from the A channel.00 . 14.12.. August. pH/mV input with 16-bit transducer for high-precision readings resolution..1800 . Teil 3 Konformitätszeichen EMC . 14. Interference emission according to the EN 61 326 standard... Germany.

sockets.1 Measurement accuracy* without sensor probe 0. Default buffer solutions according to DIN 19 266 and NBS. 128 x 128 pixels. contrast setting. or with integer pH values: pH = 1. August. Connections: A Measurement input: pH/mV input with electrode socket according to DIN 18 262 / or BNC B Measurement input: pH/mV input with electrode socket according to DIN 18 262 / or BNC KF/µA measurement input: Karl-Fischer (Dead-stop) connector for double platinum electrode (Connection sockets: 2 x 4 mm) Pt 1000 measurement input: Temperature sensor probe for P 1000 resistance thermometer (Connection sockets: 2 x 4 mm. pH=4. ph=4.00.. pH= 7.12.01.00. Allemagne 05.Translation of the legally binding german version Release 17. PH=10.2 ± 1 Digit Display: Readings display: * The measurement incertainty of the sensor probe has to be taken into account as well.sockets and 1 x 2 mm socket.connector for a Pt 1000 resistance thermometer Connection: 2 x 4 mm . 175 Display resolution 0. 4 softkeys for speedy operation of the titration unit during parameterisation and titration. August 5 .. 100 Display resolution 0. approx. 1 x 2 mm) SCHOTT Instruments GmbH Hattenbergstraße 10 55122 Mainz Deutschland. LCD graphic display with background illumination. sequence during calibration optional. Connector: 2 x 4 mm .87. 9 alphanumeric lines each 6 mm high. Germany. Measurement range T [°C] . pH=6. Automatically with 2 buffer solution out of 8. 69 x 69 mm. 3 freely definable buffers can be input.1 Measurement accuracy* without sensor probe 0...00. 4 ½ digit display with floating point and unit.00. 14 mm high. Measurement range I [µA] 0 . 5 Août 2002 AGQSF 0000-A070-00/020805 st . Additional control displays.2 K ± 1 Digit Measurement input: Karl-Fischer (Dead-stop) connector for double platinum electrode Polarisation voltage variably adjustable from 10 … 200 mV.18.2003 Measurement input: Temperature probe . Calibration: pH= 9.75 .

or a German PC keyboard.. Ohaus types. daisy-chain input RS-232-2 TitroLine alpha plus Titration Unit. HP-compatible printer with a centronics interface (parallel interface).Protocol for operating-system upload. fuse: 0.3 Volt Centronics interface (25-channel D-SUB socket) for connecting an Epson. 50/60 Hz. Allemagne 05. TM 128 and TM KF stirrer (with cable TZ 1581) Stainless steel/polypropylene. 5 Août 2002 AGQSF 0000-A070-00/020805 st . . . Part 1: 80% for temperatures up to 31 °C.TW alpha plus + TW alpha sample changers . additional types upon request Stirrer off: Voltage smaller than 1.3 Volt Stirrer on: Voltage 3.115 Volt. 2400.Weighing-scales of the Mettler.2003 Power supply: Switch-over at the bottom panel of the device .1 kg for basic device Approx. linear decrease down to 50% relative humidity at a temperature of 40 °C Printer RS-232-C-Interface: Data bits: Stop bits: Start bit: Parity: Baud rate: Stirrer control: Printer connection: Keyboard connection: StirrerConnection: Casing material: Front membrane: Casing dimensions: Weight: Environment: SCHOTT Instruments GmbH Hattenbergstraße 10 55122 Mainz Deutschland. Kern. August.25 AT. 4. 7 or 8 bits (default value 7 bits) Adjustable. fuse: 0. +40 °C for operation and storage Air humidity according to EN 61 010.Daisy-chaining is possible Adjustable.Connections: RS-232-1 computer. T universal piston burette .12. 1 or 2 bits (default value 2 bits) Fixed 1 bit adjustable.230 Volt. Germany. Sartorius. .9 for complete device including interchangeable unit (including empty reagents bottle) Ambient temperature: +10 . August 5 . Stirrer Start/stop signal for TM 135 and TM KF stirrers (TZ 1581) 12V DC out Power supply for a TM 135. height including dosing unit Approx. Keyboard PS2 socket for connecting a TZ 2835 keyboard from SCHOTT Instruments. T 200. 19200 (default 4800 baud) The baud rate can be changed via the software address (0 to 15). 4800. 50/60 Hz.RS-232-C interface with galvanic insulation via opto-coupler . .. Power consumption 42 VA .TITRONIC T 110.Translation of the legally binding german version Release 17. with interior metallization for shielding Polyester 145 x 360 x 295 mm (W x H x D). 4. per software download and upload protocol: for operating-system software and methods parameters .TITRONIC® T 110 plus piston burette. 9600. even / odd / none adjustable: 1200.5 AT.

1 % (depending on the interchangeable unit being used) Dosing accuracy of the TitroLine alpha plus Titration Unit when used with TA and TA plus interchangeable units: Interchangeable unit type no.1 ± 0. 145 x 260 x 270 mm (W x H x D) without reagents bottle Approx. Germany.0. Part 3 0. T 110/200 and T 100 plus piston burettes Automatic.05 0.05 SCHOTT Instruments GmbH Hattenbergstraße 10 55122 Mainz Deutschland.1 ± 0. August.Translation of the legally binding german version Release 17. high-grade steel.00 5. August 5 .05 0. 5 Août 2002 AGQSF 0000-A070-00/020805 st . TA 01 TA 05 plus TA 10 plus TA 20 plus TA 50 plus Volume [ml] 1.1 – 0. mounting for the glass cylinder bears unmistakable code Volume-independent cone valve made of fluorcarbon polymers made of DURAN ® FEP hose set with UV-protection To fit glass bottle to DIN 12 036 and DIN 12 039 or rectangular glass or plastic bottle With automatic checking feature Borosilicate glass.003 ± 0.3 ± 0.00 Tolerances of the Øi of the glass cylinders [mm] ± 0. Allemagne 05.15 ± 0. fluorocarbon polymers. 0.003 ± 0. acrylic glass.80 kg for TA 20 interchangeable unit (without reagents bottle) According to DIN EN ISO 8655.1 Reproducibility [%] 0. PPS.003 ± 0.10 0.05 .003 ± 0.3 % 0.00 10.12.2003 Interchangeable units Compatibility: Coding of the cylinder size: Valve: Cylinder: Hoses: Bracket for storage bottle: Fastening for interchangeable unit: Materials: Dimensions: Weight: Dosing accuracy: Correctness: Precision: The units are compatible with those of the T 100.07 0. polypropylene.00 50.00 20.003 Dosing mistakes* referred to 100 % volume [%] ± 0.

The operating voltage is specified on the type plate at the bottom panel of the titration unit. or if it is damaged maliciously or through negligence the guarantee is void. The earth wire must not be broken intentionally. it is to be taken out of operation and secured to prevent its being put back into operation unintentionally. computer. keyboard. The negligible residue of fluid may evaporate. If the unit is opened without authorisation. the user will be responsible for the safe and proper operation of the TitroLine alpha plus Titration Unit. The TitroLine alpha plus Titration Unit conforms to protection class I. X-rays or other high-energy rays are fully retained by the casing.1) When using liquids with a high vapour pressure and/or matters or mixed matters not qualified for use under chapter 1. however.2 Chapter 1 Warning and safety information Warning and safety information For safety reasons the TITRONIC T 110 Piston Burette may only be opened by authorised persons who are in charge of the observance of the relevant safety conditions. The setting can be seen from voltage selector at the bottom panel of the titration unit. These circuits (ICs) can be deleted by X-rays or radioactive rays. the fuses have to be replaced in accordance with the information on the rear panel. Before switching on it is to be ensured that the operating voltage matches that of the mains voltage. The unit must not be stored or operated in wet rooms. work on the electrical equipment may only be performed by trained specialists. The earth must not be eliminated by using an extension cable without an earthed wire.e. especially the chemical resistance of the materials of the TitroLine alpha plus Titration Unit must be taken into account (please refer to chapter 1. Please observe the corresponding operating instructions of the devices to be connected.1:1993. The mains plug may only be inserted into a socket with an earthed contact.1. i. Any discontinuity in the earth wire inside or outside the device or the release of the earth wire connection may lead to the TitroLine alpha plus Titration Unit becoming hazardous. Part 1. "Maintenance and care of the TitroLine alpha plus Titration Unit").91 1. . chapter 8. electrodes. and thus penetrate into the zone beneath the piston and corrode or dissolve the materials used there (cp. The TitroLine alpha plus Titration Unit is equipped with integrated circuits ( flash memory and EPROMs). Each time the piston rises inside the cylinder a microfilm of the dosed fluid will remain on the internal wall of the cylinder. weighing-scales. For example. When changing the operating voltage. printer. the user must observe all the instructions and warnings contained in these operating instructions. For safety reasons the TitroLine alpha plus Titration Unit is only to be used for the purposes described in the present operating instructions. When working with fluids other than conventional titration media. In order to maintain this condition and ensure safe operation. If it has to be assumed that it is not possible to operate the TitroLine alpha plus Titration Unit safely. but this has not influence on the dosage accuracy. It has been built and tested in accordance with EN 61 010 . Protection Measures for Electronic Measuring Instruments and left the factory in perfect technical condition.

please press the grey key. Screw the stand rod into the TM 135 titration rig. then fasten the stand rod bracket to the bottom panel. The desired stirring speed is set at the adjustment knob. The operating voltage is indicated on the type plate (ref. . The stirring speed cannot be controlled. push the TZ 303 electrode carrier onto the stand rod. to bottom panel). press the grey key.1.1 Set-up and first operation Unwrapping and set-up of the TitroLine alpha plus Titration Unit Functions and dimensional accuracy of the TitroLine alpha plus Titration Unit as well as that of all accessories and peripherals are thoroughly checked in the factory. Subsequently. Slip the Z 303 titration clip onto the stand rod. it can be moved easily. Power is supplied by a TZ 1581 connection cable. If the stirrer is to be switched on/off from the titration unit. 2.1. To complete. To do so.92 Chapter 2 Set-up and first operation of the TitroLine alpha plus Titration unit 2 2. Prior to switching the device on. The TM 135 magnetic stirrer is attached to the bottom of the titration unit using the appropriate TZ 1560 connection sheet. 2. To begin. Power is supplied by a TZ 1581 connection cable. Please pay attention not to leave any of the small accessories in the package. To do so. 2 Working without titration rig: The TZ 1516 type no. To do so.1 Installation of the stand rod − − − Fig. lay the titration unit on its left side.1. push the TZ 303 electrode holder onto the stand rod. screw in the stand rod. After pressing the retention key. 2. please refer to the enclosed parts list.2 Connection of the optional TM 135 titration rig.2. For the scope of delivery. Working with the TM 135 titration rig: please refer to chapter 2. it has to be ensured that the operating voltage of the device matches that of the mains voltage. fasten the connection sheet to the titration rig. The TitroLine alpha plus Titration Unit may be placed on any flat surface. The mains supply cable plugs into the cold-device socket at the back panel of the device. then attach it to the bottom panel of the titration unit.1. the second TZ 1581 connection cable is to be used.3 Installation of the titration clip Subsequently. stand rod bracket is mounted to the bottom plate of the titration unit using the screw.

Fill the solvent into the 1 l solvent bottle.93 2. so no molecular sieve will fall into the titration vessel. unscrew the large screwed connector. and then put one of the enclosed septums into the opening. Adjust the dosing and waste hoses as is shown in figure 3. item 10 and 6). If you wish to inject liquids through a septum. You can remove the black O-ring before. All the other hose connectors as well as the threaded connections of the TZ 1770 or TZ 1772 titration vessel are already pre-mounted. This applies to the titration vessel. item 9) using one of the short PVC hoses (figure 3. Screw the titration hose onto the valve of the titrator. Connect the other hose olive of the desiccation bottle to the hose olive of one of the square bottles (solvent bottle) using the other short PVC hose (refer to figure 3. you will place the titration rig directly to the right of the TitroLine KF Titrator. tighten the screwed connections with the hoses on the titration vessel and on the bottles. Insert the stand rod (figure 3. fill some molecular sieve into the desiccation pipe for the titration vessels. First. the storage bottle.2 Chapter 2 Set-up and first operation of the TitroLine alpha plus Titration unit Installation of the TM KF Titration Stand and of the titration vessel As a rule. Also. . item 2). item 4) into the corresponding holder on the titration rig. Seen from the front: Connect the right hose olive located at the back panel of the titration rig and the hose olive on the desiccation bottle (figure 3. Use the knurled screw to fasten the titration vessel to the stand rod in such a manner that the bottom of the titration vessel sits directly on the upper surface of the titration rig. Connect the left hose olive at the backside of the titration rig and the hose olive on the waste bottle using the longer PVC hose (figure 3. and the waste bottle. you should put some of the enclosed cotton into the desiccation pipe. item 9) up to two thirds with the enclosed 0. tighten the screw connector manually. *) Fill the desiccation bottle (figure 3. then tighten it manually. Instead of the square laboratory bottles coming with the device.3 nm molecular sieve. Subsequently. Subsequently. item 10 and item 3). it is also possible to use other reagents bottles with a GL 45 or S 40 (TZ 1795 adapter) thread.

3 Hose connections (seen from behind) 1 2 PTFE filling hose: Storage bottle ⇔ titration vessel PVC hose for overpressure in the storage bottle: Storage bottle ⇔ receiver drier PVC hose for overpressure in the storage bottle: Receiver drier ⇔ titration vessel Stand rod PTFE suction hose: Titration stand ⇔ waste bottle PVC hose for vacuum in the waste bottle: Titration stand ⇔ waste bottle GL 45 screwed connections with adapter for hose connections and screw connections Storage bottle 1 l. brown Receiver drier 100 ml *) see previos page Olives for connection of the PVC hoses Mains-unit connector U = 12 VWaste bottle 1 l. bright 3 4 5 6 7 8 9 10 11 12 .94 Chapter 2 Set-up and first operation of the TitroLine alpha plus Titration unit Fig.

or if the pressure or vacuum systems should contain a leaking screw connection. Prior to extracting the finish-titrated solution. The built-in magnetic stirrer (stirrer for magnetic stirrer rod) will stir the liquids in the titration vessel. Likewise. if solvent should coast out of the storage bottle during the change from dosing to extraction. Note: Wrinkled hoses will cause dosing and extraction problems. Note: Please observe the filling level of the waste bottle. • suck off: Press the back part of the key rocker to suck off solvent into the waste bottle. If solvent should coast out of the storage bottle during additive dosing. please place the bottle on a level below that of the titration vessel.95 Chapter 2 Set-up and first operation of the TitroLine alpha plus Titration unit Power supply of the TM KF Titration Stand The power supply to the titration stand comes from the titrator. So when checking the hoses. item 11 and figure 4. The tightness of the hoses including their connections should be checked on a regular basis. 4 Dosing Extraction Stirring speed Malfunction Problems might occur if the hoses were connected improperly. Working with the titration stand • Dosing: Press the front part of the key rocker to pump solvent into the titration vessel. Fig. 12 V DC). The same applies to the hose connections of the receiver drier or the interchangeable desiccating tube unit. Use theturning knob on the front panel of the titration stand to set the stirring speed (figure 4). Connect the titration stand to the titrator using the enclosed low-voltage cable (please refer to figure 2. . sucking off will continue as long as you press the key. This will lead to a cessation of the solvent discharge after only a few seconds after the start of the pump. please pay particular attention to the tightness between the flanged connections and their adapters. Replace defective items if necessary. you should wait for a few seconds during the change. Additive dosing will continue as long as you press the key. please ensure that the waste bottle can accommodate at least this quantity of solution.

3 Chapter 2 Set-up and first operation of the TitroLine alpha plus Titration unit TA plus Interchangeable Unit: 11 1 2 12 10 9 3 8 7 6 5 4 Fig.protection Receptacle for reagents bottle according to DIN 12 036 and DIN 12 039 or rectangular glass or plastic bottle Adjustment of reagents bottle Suction hose to valve Clamped screwing connection FEP hose for valve ↔ cylinder connection Items 1.96 2. . 2. and 12 are only available within the whole set. 11. 3 1 2 3 4 5 6 7 8 9 10 11 12 Built-in valve TZ 3000 with automatic control Hose between valve an titration tip Titration tip Inscription field Locking feature with lower burette section Cylinder pressure screw UV. 10. 3.

4 Chapter 2 Set-up and first operation of the TitroLine alpha plus Titration unit Fitting and replacing an interchangeable unit An interchangeable unit is installed by simply slipping it on over the guide rail from front to back. If a new interchangeable unit is installed without reagent. Caution: After each replacement. you have to initiate an "Initial filling" or "Rinsing" of the Piston Burette (please refer to chapter 2. Subsequently. 4 . 4). an automatic filling plus a float balancing will take place. until the top edge of the PTFE piston is approx. The actual adjustment is now made by simply pressing the interchangeable unit onto a flat surface. please use the clamping ring provided to prevent the reagents bottle from falling out of the holder. Fig. the interchangeable unit can be easily removed towards the front by pressing the retention key on the left side of the unit (Fig. To adjust the piston. but only as soon as the device is again ready for the start of a titration.4). it is to be drawn downwards using the piston drawer provided. please DO NOT remove the interchangeable unit immediately. the signal tone will sound. If you have problems during installation.4. one has to initiate the filling operation (also refer to chapter 2. the piston in the cylinder has to be set to the lower starting position. "Initial filling and rinsing of the entire interchangeable unit"). for example a table top. 8 mm below the upper edge of the black pressure screw. The installation is completed when you can clearly here the mechanical retention device snap in. This ensures accuracy of the dosed volume at any time. For this reason. After removing the interchangeable unit. The front parts of the interchangeable units contain a coding which communicates the size of the installed cylinder to the bottom section of the burette. After inserting a different (or the same) interchangeable unit the burette size is automatically recognised and displayed.97 2. To replace an interchangeable unit. and the display <<--->> indicates that no interchangeable unit is presently in place. When working with a square glass bottle. the position of the PTFE piston in the glass cylinder has to be adjusted. To do so.

The cylinder with the piston inside can now be removed. The UV protection is to be pulled down. item 12). the hoses and cylinder contain chemicals which may escape or be sprayed around when the unit is dismantled. 3. it is to be ensured that only the intended cylinder size is fitted within an interchangeable unit.98 2. item 6) for the glass cylinder is to be loosened by turning it anti-clockwise and pulling it downwards. 3. A new cylinder with a piston is to be fitted in reverse order and then adjusted as described under chapter 2. Fig. and the black press screw (cp. it is always recommended to replace the PTFE piston at the same time when a defect glass cylinder is replaced.5 Chapter 2 Set-up and first operation of the TitroLine alpha plus Titration unit Replacing the glass cylinder The hose between the cylinder and the valve is to be unscrewed from the cylinder (see Fig. For reasons of dosage and analysis accuracy. Please note: As a rule. The relevant safety precautions for dealing with these chemicals have to be observed. This applies in particular if the glass is broken. The result would be an incorrect dosage. Basically.3 "Fitting and replacing an Interchangeable Unit". 5 Installation of the glass cylinder . Fig. since the sealing rings of the PTFE piston may be damaged by splinters of glass. since otherwise the code in the interchangeable unit will not conform with the cylinder size.

and liquids which widely differ in composition and/or concentration. and titration tip (for disassembly procedure. . the filling procedure is started during which fresh reagent is drawn in. the contents of the cylinder (air or old reagent) will be ejected. After the execution of the program. with fresh reagent being taken in.6 Chapter 2 Set-up and first operation of the TitroLine alpha plus Titration unit Initial filling and rinsing of the complete interchangeable unit Initial filling of the burette unit is carried out by a rinsing program.4. When the lower piston position is reached. and the display will show <<Status: Ready>> Please note: The initial filling / rinsing program is not suitable for changing titration media. the display will show <<Status: Rinsing>>. Subsequently. glass cylinder. Such cases require a careful cleaning of the parts coming in contact with the titration agents. During the execution of the first-fill or rinsing program you have to place a waste container under the titration tip. solutions. respectively. the TitroLine alpha plus Titration Unit is ready for the next titration or dosing processes. You initiate the rinsing program using the keyboard in the <R/D> menu item. the complete contents of the cylinder will be ejected once again. and depending on the setting. “Replacing a glass cylinder”). and after float balancing the message <<0. and the piston will move to its bottom position. valve. please refer to chapter 2. the appropriate number of upward and downward piston strokes will be carried out. or <<0. As soon as the top piston position is reached.99 2.00 ml>> with interchangeable units of 20 and 50 ml. Another filling cycle will take place. such as intake tube.000 ml>> will appear in the case of interchangeable units from 1 to 10 ml. After the start of the rinsing routine. After the start of the rinsing program.

T 110 plus TW alpha plus T 110. PG weighing-balance etc. length 10 m Daisy-Chain. TM 128 TM KF Please note: If a keyboard. . please refer to chapter 5. For a functional description. HP-Deskjet 920 / 970 etc. please refer to RS 2 RS 2 TitroLine alpha plus. Centronics Printer e. Keyboard keyboard Stirrer TM 135.7 Chapter 2 Set-up and first operation of the TitroLine alpha plus Titration unit Combining the TitroLine alpha plus Titration Unit with other devices The TitroLine alpha plus Titration Unit may be combined with numerous devices of the SCHOTT-Instruments programme. TW alpha T universal Sartorius weighing-balance Mettler AT weighing-balance Mettler AB-G. T 200. Epson Stylus Color 880/980. please note the information provided with the devices being connected to the TitroLine alpha plus Titration Unit.25 115V 0 5 I O 2 KeyStirrer 12V out Fig. length 5 m or with cable. the titration unit has to be switched off and on again in order to star the initialisation of the system. please refer to the table below: KF Pt Electrode A Electrode 1 RS-232-C Printer Ref Centronics Ref 230V:0. “Working with the TitroLine alpha plus Titration Unit”. or weighing-balance is connected to the TitroLine alpha plus Titration Unit. 6 Keyboard Stirrer Printer Cable TZ 3088 TZ 3089 TZ 3084 TZ 3086 TZ 3087 TZ 3082 TZ 3083 TZ 3081 CentronicsCable TZ 2835 TZ 1581 TZ 1581 What is to be connected? RS 1 PC with cable.g. printer. In addition.100 2.

the TZ 303 electrode holder and one measurement electrode. the TZ 2835 keyboard.101 Chapter 3 Construction of the TitroLine alpha plus Titration unit 3 Construction of the TitroLine alpha plus Titration Unit Fig.7 The illustration shows the TitroLine alpha plus Titration Unit with an interchangeable unit TA 20 plus. . a TM 135 magnetic stirrer.

B input channel . A maximum of 1. The B measurement input is galvanically insulated from the measurement channel A. 4. 5 s Suitable for titrating media aqueous. The smoothing of the measurement signal cannot be adapted any further in the case of the B pH/mV measurement input.2 Measurement-amplifier. the two measurement potentials must not be connected to each other.000 measurement values can be recorded during one titration. the A measurement input has to be used. electrode signal The B input channel of the measurement amplifier enables another a pH/mV electrode to be connected to the TitroLine alpha plus Titration Unit . 99% of the measurement signal are obtained only after five seconds.1 The connections of the TitroLine alpha plus Titration Unit Measurement-amplifier. In the case of the pH/mV A channel. If more measurement values are present. The respective setting times of the four smoothing stages are as follows: This means that the input delay causes a delayed response time of the input amplifier. If the measurement signal needs to be smoothened. they will be stored through an automatic data reduction. the minimum setting time has to be ensured by suitably long waiting times.102 Chapter 4 The connections of the TitroLine alpha plus Titration unit 4 4. the smoothing of the measurement signal can be adapted in four stages to the requirements of the titration.5 s approx. acid-base titrations in halogenised solvents or aromatics The selectable input delay (setting time) causes a smoothing of the measurement signal. at the start of the titration. This is done through the user surface of the TitroLine alpha plus Titration Unit. alcoholic solvent with non-polar portion non-aqueous. This ensures that the two measurement signals will not affect each other. 0. A input channel . . in the case of smoothing stage 3 (5 s). 2. . non-polar Examples Acid-base titration in water Acid-base titration in alcohol and weak acids or bases in water Titrations in alcohol/water mixtures TAN. electrode signal The A input channel of the measurement amplifier enables a pH/mV electrode to be connected to the TitroLine alpha plus Titration Unit . e. TBN. polar aqueous. one should work with a fixed drift control. In the case of a very high variation of the measurement values. with the delay time being been at least as long as the setting time resulting from the selected input delay.5 s approx. For instance.g. Smoothing stage 0 1 2 3 Input delay none weak medium strong Setting time 0s approx. To maintain this galvanic insulation. For this reason.

it may be deactivated retrospectively. Deletion of the input digit/character above the cursor. the keys of the keyboard supplied with the unit have the predefined functions as specified in the table below: Start of the method being displayed Cancellation of the method Cancellation of calibration Direct move to method creation Filling of the dosing unit The current titration parameters are stored. Scrolling within the overview menu to select specific menus Within the specific menu items: Move the cursor to select parameters Input of method numbers. It is also possible to display the current measurement value and the consumption instead of the on-line curve. sample numbers. respectively.103 Chapter 5 Working with the TitroLine alpha plus 5 5.9 decimal point <ENTER> or <↵> <DEL> or < ←Backspace > Letters. upper case only.> <FILL> <STORE PARAM. such as an on-line curve during titration.. It can be used to display charts. ASCII characters All other keys <START> <STOP> <EDIT PARAM.1 Working with the titration unit Functioning of the keyboard In combination with the TitroLine alpha plus Titration Unit. Within the present operating instructions. If nothing visible is left on the display. Confirmation of the input parameters. please press these keys until something becomes visible. and parameters. Without any function. the previously specified pH or mV end value will remain stored within the respective method and use for all future scaling operations. (The display can be switched over during titration using the <III> key. and you return to “Method creation”. With the MF keyboard. Display of the <MODE> overview menu to parameterise a method. both upper and lower case. .> <MODE> <INIT> <CAL> +/DOS <ESC> returns you to the previous menu level. Jump to the system parameters menu item Display of the <Calibration> menu for calibrating the A and B measurement channels Change of sign Takes you to the “Dosing” menu <F1> <F2> <F3> <F4> <F5> <F6> <F7> <F8> <F9> <F10> Num Lock Prt Sc Sys Rq Scroll Lock <ESC> < ↑> < ↓ > Cursor key 0. The scaling of this graph depends on the end value which was specified as an end criterion in the case of a pH or mV titration. both keys are referred to as <ENTER>..) • Contrast setting: The contrast is set using the Page-up or Page-down key. However. Multifunctional graphics display: • • The display is a LCD graphics display with a resolution of 128 x 128 pixels. ASCII characters  Alphanumeric input is possible. If the pH or mV end criterion is supposed not to appear within a method. Letters. In the case of TZ 2825.

The parameters are inquired and input in a dialogue. This kind of inquiry is particularly user-friendly. you can stop running titration. they are designed to facilitate the device’s parameterisation during input. or jump back to the preceding selection menu Upward selection Downward selection Take over value (ENTER) Start calibration Parameterise. and rinsing processes.104 The menu system: Chapter 5 Working with the TitroLine alpha plus Head line Line 1 Headline Line 2 Input Line 1 Input Line 2 Input Line 3 Input Line 4 Input Line 5 Input Line 6 Input Line 7 Status Information 003 01 • The display is divided up into 9 lines. since after you will always be returned to the starting point each pass. in this way it is impossible to get lost. On the right side you can see four “softkeys” which are assigned to the keys as a function of the menu level you are currently on. dosing. with the two upper heading lines containing information about the currently active user-menu level. One step backwards at input. dose START/STOP: Start of titration Using STOP. The lowest line shows “status” informations. . rinse.

) output: Printout and export of the curve and the titration results of the last titration. The desired method is selected by pressing the <↑>/<↓> keys. The mode menu can be used to input all settings required for parameterisation. etc. New calculation: A re-calculation will be performed. copy and export methods.2 • Chapter 5 Working with the TitroLine alpha plus The start menu In principle. This level can only be accessed using a connected keyboard. if necessary with a modified formula. it is always possible to adapt the TitroLine alpha plus Titration Unit to your specific requirements. From the level of the Start menu. Pressing the <C> key will initiate the calibration of the 5.3 • • • The mode menu <MODE> / <F6> is used to select the mode menu. print. new: Creation of a new method: system settings: Used to select the system parameters of the titrator measuring: Performance of measurements (pH. Titroline ready no. or for a change or the administration of methods as well as for the input of calculation and documentation parameters. 1 01 • Method 1 dosing unit size 20 ml 001 Start menu: The method which is available is always the one which was being worked with before the device was switched off. the corresponding method name will be displayed.105 5. mode change 1 method method management create method system settings measuring output new calculation 01 • • • • • • change method: This item can be used to change an existing method. 100 default standard methods for various applications quickly and conveniently. mV. management: Selection of standard methods. you can modify each method in such a manner that it will fit to your very specific tasks. delete. The rinsing and dosing parameters and the rinsing and dosing functions can be set after pressing the <R/D> key. you also have the possibility of accessing the database containing approx. The specified documentation will be prepared once again 003 • . This menu may also be used to access the system settings. titration is started by pressing the <Start/Stop> key. After entering the desired method number. Below you find an overview of the major menu items. Yet. In this context. The upper right corner shows the method which is presently active. as well as printout and display of the CAL data.

If the selected method is to be changed. Titration parameters: Selection of the titration type and the parameters: selection of the titration type (pH. it is possible to link several methods at this point. or administration of formulae Documentation: Selection of the type of documentation reset statistics?: This item can be used to reset the statistics memory of the current method.1 Chapter 5 Working with the TitroLine alpha plus Changing a method 01 • To activate the password feature. Hardware settings) method creating Passwort Passwort eingeben • 048 new method method number 1 enter method name Methode -----1 01 • • • At this point a 18-digit alphanumeric method name can be input. • 049 method creating selection sample handling/TW titration parameter calculations documentation reset statistics? method link 01 • Sample handling/PW: This item is used to enter the sample ID. Method link: With more complex jobs. inquiry. the type of the sample quantity. You may enter a different method number. pressing the <Enter> or < > key will take you to the following menu item. or for the creation. µA.3.5.3. please use the “Password” item of the system menu (please refer to chapter 5. mV. The <↑> <↓> arrow keys will take you to the next menu line. Karl-Fischer or pH-Stat) Calculation: This item is used to calculate the parameters of the selected method. and the kind of sample changer. • • • • 032 • .106 5.

speed) 106 Calculation: Calculation of the results 107 a. the system will take you directly to the menus used for the input of the sample description and the sample weight or volume. the TL alpha sample changer will be programmed to the selected plate size. sampler none 12 samples 16 samples 24 samples 24 samples COD 01 • If no sample changer is selected. If COD titration is to be performed. the first thing to do is to select a plate size. please select the <<24 samples COD>> position. sample ID. A method is not completely defined and parameterised unless or until all the above settings have been made. Documentation: Output of the titration including result 441 Statistics reset: Reset of the statistics memory of the current method. If the use of the TW alpha sample changer is specified. respectively. sample changer 65 Titration parameters: Control of the titration (type. Sample parameters/TW menu item At this point a decision is made as to the use of a sample changer and the processing of the sample. This is the only menu which can be used to influence the attribution of sample descriptions and the selection of a sample quantity or sample volume. 108 Method interlinkage: Run next method automatically. sample quantity. * The numbers are equivalent to the numbers of the menues displayed. • • 050 . At the time the method is started.107 Chapter 5 Working with the TitroLine alpha plus Overview of all elements of a method 032* 50 Sample parameters/TW: General settings.

None: No sample quantity or sample volume is desired • • • 179 • • Parameterisation of the sample quantity (sample weight) titration parameter weight fix weight in g: 1.0 will be substituted in order to avoid a division by 0 in the calculation. 1. a value has to be input at this point. The quantity or volume. will be multiplied by the factor "Q". Sample volume fixed: The sample volume entered here will be used for each titration within the method. the input mass is used for each titration. the balance data transmitted from a connected balance will be taken over automatically. a master value is to be input which will then be counted upwards automatically. If no value is input. Sample weight fixed: Within the method.00000g 01 • At this point. without the possibility of entering a sample ID • • 079 Parameterisation of the sample quantity (sample volume/sample weight) titration parameter sample amount weight manual weight automatic weight fix volume manual volume fix no 01 • Sample weight manual: The weighing-in data which are inquired at the start of the method have to be input manually each time. Sample volume manual: The sample volume has to be input manually after the start. Automatic: Only active if a sample changer is being used. a value has to be input at this point. 1. Sample weight auto: Upon the start of the method. If selected. At the beginning. The sample weight entered here will be used as a fixed sample weight for all subsequent titrations of this method. the value for the desired sample quantity is to be input. None: Titration will start directly.0 will be substituted in order to avoid a division by 0 in the calculation. If no value is input.108 Chapter 5 Working with the TitroLine alpha plus Parameterisation of the sample ID titration parameter sample ID manual automatic no 01 • Manual: A manual sample ID is prompted for input upon the start of a method and has to be entered using the external keyboard. respectively. 203 . The calculation will take into account the sample quantity or volume if the formula contains a "Q". If selected.

000 ml 01 • At this point. 1 01 • If more of than one determination is to be made on one and the same sample. when using high-walled COD containers. 104 • . The sample volume entered here will be used as a fixed sample volume for all subsequent titrations of this method. you will be taken to the input of the desired stirring speed after selecting the plate size to be used: titration parameter stirrer speed step 1-10 enter value : 7 01 Please note: Proper mixing of samples prior to the start of the titration will have a direct influence on the titration result! • • You can select one out of 10 possible stirring-speed stages.109 Chapter 5 Working with the TitroLine alpha plus Parameterisation of the sample quantity (sample volume) titration parameter volume fix volume in ml: 1. you will be taken to the following menu item: titration parameter number titrations multi determinat. The results obtained are used to perform a statistical calculation of the mean value and the standard deviation as soon as a value greater than 1 is entered. this is the point to input the number of determinations to be made. A value of 6. has to specify the formula to be used for the preparation of the statistics! • • 208 Parameterisation of the sample changer If a sample changer is to be used. The calculation. The stirring speed on a connected TM 135 is to be set manually. The stirring speed refers only to the stirrer in the sample changer. for instance. 10 seconds. 204 Input for multiple determination (statistics) As soon as you have made the desired settings for the balance data and the sample volume. Likewise. however. makes sense Please note: When using a TW alpha sample changer with stirrer. since this stirrer has a startup time of approx. a minimum stirring time of 15 seconds should be set from below. the value for the desired sample volume is to be input. the optimum minimum stirring time should be determined in experiments.

Using rinsing positions. the required parameters for controlling the various rinsing operations are inquired one after another. the positions 15 and 16 are rinsing positions. the number of the desired rinsing positions is inquired. capacity of the titration vessel should be observed. the rinsing liquid may spill over the titration vessel! If desired. The possible rinsing positions always refer to the last three plate positions. the max. you can define a position in which the electrode may remain. it makes sense after finishing titration on the first sample to replace the first sample by a KCl solution and to define the first position as "waiting position". 99 s). In practice. As a rule. At this point.110 Chapter 5 Working with the TitroLine alpha plus Parameterisation of the rinsing function If the rinsing function is selected. After the titration of the last sample. WARNING: Please note the capacity of the titration vessel!! One position/two/three positions. 055 . for instance. Considering that in this case rinsing is done at the titration position into the sample on which titration was just finished. • 051 Once the rinsing type or number of desired rinsing positions selected. sampler waiting position first position second position no wait position 01 • In order to avoid that the electrode will dry out after titration with its head up. the optimised rinsing time depends on the application. 052 Warning: If the selected rinsing duration is too long. If. you may subsequently determined the waiting position of the electrode. in the case of a 16 sample plate. for instance a TP 20. the rinsing duration will determine the rinsing volume. 5 seconds should be sufficient. the sample changer will move to the waiting position and immerse its head into the beaker glass. sampler rinsing do not rinse rinse with TP one position two positions three positions 01 • • Do not rinse: No rinsing will take place Rinse with TP: If you connect a rinsing device. two rinsing positions are desired. for instance over night. sampler rinsing time rinsing time in s : 5s 01 • If a rinsing device is connected. you set the rinsing duration (max. you have the possibility of rinsing the measuring sensors and burette tips at the titration position.

the number can be re-defined for each titration process. the parameterisation of the processes of the sample changer is completed. • µA titrations are performed using a double platinum electrode and in the form of µA deadstop titration (e. Once sample processing has been parameterised. and you can proceed with the parameterisation of sample processing. 1 • 057 At this point. The number of the samples to be processed can be specified within the method. bromine index). the parameterisation of the titration as such starts. • KF titration is used for water determination with special equipment.1. please refer to "Sample description". The number will be inquired after each start of the method. - 065 . In the case of method interlinkage it is possible to enter different starting positions. 5.1 Titration parameters menu item pH/mV titration type The parameterisation of mV titration is done in analogy to pH titration titration parameter type of titration selection pH selection mV selection uA selection KF selection pH-Stat 01 - The present example illustrates the selection of pH or mV.3.g. 056 sampler number samples variable number samples 01 • With the <<variable>> setting.111 Chapter 5 Working with the TitroLine alpha plus sampling assign position start position 1 01 • The starting position may be specified explicitly. • pH Stat is a form of titration where a defined pH value is kept constant over a specified period of time.

a specific drift value has to be fulfilled.5 s Medium: The signal will be delayed by approx. The drift value is defined in terms of reading/time and is defaulted internally in the form of quite a number of experiencebased applications. 2. for instance with titrations in non-aqueous solutions.5 s Strong: The signal will be delayed by approx. you may select whether an initial measurement value is to be recorded prior to the start of titration.0 s • 248 . This is particularly beneficial in the case of titrating solutions with a high Ohmic resistance. 244 Selection of the measurement channel titration parameter select channel measuring channel A measuring channel B 01 • • A measurement input: Channel for pH and mV titrations. 5. The B measurement channel is galvanically separated from the A measurement channel. An initial measurement value will be recorded "exactly" and also documented automatically. The individual stages corresponds to the following timely delay (for a signal change of 99%): None: The signal will not be delayed Weak: The signal will be delayed by approx. value yes no 01 • • At this point. 0.112 Chapter 5 Working with the TitroLine alpha plus Initial measurement value titration parameter initial meas. Before an initial value will be recorded. B measurement input: Channel for pH and mV titrations. 246 Attenuation of the A measurement channel titration parameter input delay no / water weak / alcohol medium / organic strong / unpolar 01 • These parameters are used to determine the response time of the input amplifier.

for instance. since the dosing volume is optimally adapted to the curve. • • 068/ 250 • • pH/mV end point titration titration parameter control EP 1 strong medium weak buffered minimal consumption 01 The parameters for end point titration depend on the type of the curve: • • • • • Strong: Strong jump with a steep rise Medium: Medium jump with less steep rise. In this case the dosed volumes of the individual dosing steps are different in their magnitude. The disadvantage of linear titration control is found in the range of the equivalence point. such as can be found in paper industry. larger dosing steps may be performed in a more flat curve portion. irrespective of the change of the measurement signal. Linear: Titration is performed with constant dosing steps. less measurement points will be recorded in the flat part. As a result. and by the drift. This means that the volume to be dosed will double up to the maximum volume. The step distance depends on the respective rise of the titration curve. This is required for special methods. This kind of titration is subject to a given regulation which will be influenced by the curve to be expected. as a rule. with tartaric acid Minimum consumption: E. since. for instance. which means titration will be finished as soon as the measurement value which was input as end point will be reached. If the titration curve becomes steep.g. for instance. whereas more measurement points will be recorded in the steep part of the curve. only very few date points are found there Dynamic: Dynamic titration will be performed. for instance.113 Chapter 5 Working with the TitroLine alpha plus Subsequently. titration is performed to TWO pre-defined end points. As a result hereof. with acetic acid Weak: Flat jump without significant rise Buffered: A flat curve portion ahead of the end point as is the case. This feature means that a dynamic titration will be performed considerably faster than a linear one. dosing will continue at minimum volume until the set value is reached. for p + m valued titrations. you select the way in which the titration is to be performed: Selection of titration type (pH/mV) titration parameter pH titrations one end point two end points three end points linear (EQ) dynamic (EQ) 01 • One end point: Titration will be carried out to ONE predefined end point. This kind of titration may be used. Three end points: Titration is performed to THREE predefined end points. for the titration of blank values 252 . Two end points: In this case.

the control and the speed of titration are to be parameterised. Cycling is done separately for each end point.00 steps[ml] 0. titration parameter EP speed fast medium exact value: 100 ml/min 01 • Fast / medium / exact: These criteria influence the duration of titration. 255 After the definition of the titration end. a measurement value drift per minute will be indicated. The drift control feature ensures the quality of the measurement values. you may select the various end criteria as a function of the pH/mV value. If <<value>> is selected. These menus are cycled through up to three times as a function of the desired number of end points. It defines the time when a measurement value will be taken over after the addition of the titration agent. after the end point was reached for the first time. titration parameter enter value EP 1 end[pH]: 7. 209 . accurate titration will take longer (approx.114 Chapter 5 Working with the TitroLine alpha plus Subsequently. it will be used as a safety criterion to prevent the titration vessel from spilling over. As a rule. which means that an "accurate" drift control will cause a stable measurement value and thus an enhanced certainty of the measurement value.00 ml delay[ s]: 10 s 01 • • • End [pH]/[mV]: Titration is performed up to an input pH/mV value. <<medium>>. 4 to 5 minutes) Under the 'value' item you can enter a drift which has to be fallen short of in order to end to titration • 265 titration parameter total consump.00 ml 01 • The ml end criterion will define the cancellation criterion for titration.ml 50.titr. so that titration will be ended. but also a slower course of the titration. respectively. <<accurate>> parameters have an influence on the titration duration owing to the drift control. End point titration will be performed at various speeds: Fast titration will be finished within less than one minute. Step [ml]: At the end of titration. Delay [s]: Delay time upon the lapse of which the input end point has to be exceeded or reached. The selected <<fast>>. final consumpt.

irrespective of the course of the curve. a combination of criteria is used. and therefore it should always be input. • 268 . titration parameter end of titration mL EQ M Val M Val + mL M Val + EQ mL + EQ mL + M Val + EQ 01 • • • mL: Titration will be ended as soon as the input ml value has been reached. will ensure that the beaker glass will not spill over. Decreasing: The initial measurement value is above the end point. titration parameter linear steps step size in ml 0. titration is done in constant dosing steps and irrespective of the change of the measurement value. for instance. The volume being dosed remains the same all the time. Automatic: Titration starts without any verification of the initial measurement value. A great many titrations in organic media are performed in this way (mostly in combination with fixed waiting times). EQ: Titration will end as soon as the specified number of EQs has been reached (inflection point of the titration curve).115 Chapter 5 Working with the TitroLine alpha plus Increasing/decreasing titration After the input of the titration parameters for end point titration. An ml criterion. The above criteria are intended to prevent an end point titration if the end point has already been exceeded. 071 Parameterisation of linear titration: In the case of linear titrations. The measurement value depends on the specified titration type (pH or mV). The first criterion which is reached will end titration. M Val: Titration will be ended as soon as the specified measurement value has been reached. 096 Combining multiple end criteria If you selected linear or dynamic titration. this menu item will give you the opportunity of selecting and combining multiple end criteria. you specify the expected direction of the titration: titration parameter titration direction increasing decreasing automatic 01 • • • Increasing: The initial measurement value is below the end point.00 ml 01 • Enter the desired volume in ml. Titration will always be performed with identical steps. M Val + mL / M Val + EQ / mL + EQ / mL + M Val + EQ: In practice.

this is the point to select the sensitivity of the inflection point to be determined. The values vary from 1 (weak jump) to 1000 (strong jump). 274 EQ end criterion. As a hint strong jump: > 300 medium jump: > 10 weak jump: < 5 275 .00 ml 01 • Enter the desired value in ml 092 EQ end criterion titration parameter number end points EQ 1 EQ 2 EQ 3 EQ 4 EQ 5 01 • This item is used to specify the desired equivalence points at which titration will be cancelled. titr. Special titrations may require an adaptation of the cancellation criteria in the form of an input of a numerical value for the derivative. final consumpt.116 mL end criterion Chapter 5 Working with the TitroLine alpha plus titration parameter total consump.ml 50.00 01 • • Depending on the intensity of the jump. 8. type of jump titration parameter end of titration steep jump medium jump flat jump value dev.

final consumpt. This bears the disadvantage that the duration of titration is frequently longer than with the other drift criteria. that the titration vessels volume will not suffice. Fixed waiting time: The waiting time following the addition of the titration agent is always constant. non-aqueous titrations such as TAN/TBN. Fast/medium/exact: The measurement value resulting from an addition of titration agent will always be taken over as soon as it has stabilized over a certain period of time.00 01 • The measurement value to be input here depends on the specific titration type: pH. titr.e. 269 Selecting drift control titration parameter drift control fixed waiting time fast medium exact set 01 • • Drift control decides when a measurement value is taken over after the addition of the titration reagent. mV. PLEASE NOTE: In the case of difficult. 271 mL + EQ end criterion titration parameter total consump. In the case of acid/base titration in aqueous media.00ml 01 • At this point you should enter the maximum volume upon reaching of which a titration is to be cancelled. i. Set: The value of the drift may be set at this point in terms of mV/min.117 Chapter 5 Working with the TitroLine alpha plus Measurement value (M Val) end criterion titration parameter enter end M Val: enter parameters 0. µA. ml 50. • 097 • . This criterion is used to prevent excessive titration. the selection of ‘fast’ or ‘medium’ as a criterion is recommended. or inhibited chemical reactions such as the determination of aromatic amines through diazotising it is necessary to set a constant waiting time.

.00s 01 • Waiting time between two titration steps in seconds Can be set in steps of 1 second up to 999 s. 098 Input. value of drift control titration parameter drift control set 100.the slope (please refer to the 1st derivative) where the largest step width is to be titrated.the largest step width for the flat portion of the titration curve .118 Chapter 5 Working with the TitroLine alpha plus Input.the slope (please refer to the 1st derivative) where the smallest step width is to be titrated. fixed waiting time of drift control titration parameter fixed waiting time waiting time in s: 0. application-specific settings . 276 Dynamic pH/mV titration titration parameter dynamic control steep jump medium jump flat jump Dyn_Parameter 01 The selection of the type of jump has an influence on titration control and will improve the result of titration.00 [mV/min] 01 • Special requirements of the application may make it necessary to enter a value here in terms of mV/min. • • • Steep leap. such as in the case of chloride with silver nitrate. Flat jump.the smallest step width for the steep jump range . such as in the case of hydrochloric acid with NaOH Medium jup. 099 . such as in the case of trace determination of chloride Dyn_Parameter: This is the point to make detailed.

TITRONIC® 200. it is possible to run a stirring time. burette ext adress burette: 00 01 • • This item is used to input the address which is set as address on the desired external burette. The starting stirring time (0-999) proceeds prior to the initial measurement value and pretitration.01 100 ml) at one time.00 ml 01 • Pretitration is intended to add a larger dosage volume (0. titration parameter titrat. or by means of the rotary switch at the back panel of the device.119 Chapter 5 Working with the TitroLine alpha plus Input of the start stirring time (waiting time 1) titration parameter start mix time [s] enter value : 1 s 01 • Prior to commencing titration. piston burettes of the TITRONIC® universal. 279 The external burettes which may be used include. durchmischen. The illustration below shows the inquiry of the address for the desired external titration burette. for instance. The added dosage volume will be taken into account in the result of the titration volume. This stirring time can be used for mixing a sample properly. Please note: In the case of pH Stat and Karl Fischer titrations the use of an external titration burette is impossible for system reasons. and to accelerate or optimise titration. Using the "titration burette <yes>/<no> menu item you can select whether you wish to work with an external titration burette. 074 External titration burette You have the possibility of titrating using an external burette. and TITRONIC® 110 plus types. depending on the type of the burette. TITRONIC® 110. Pretitrating titration parameter pretitration pretitration in ml: 0. In the case of EP titrations the titration speed has to be set to less than 4 ml/min (20 ml interchangeable unit) or to ‘accurate’. 280 . The address of the external burette is set either using the software.

this is performed using an external burette.120 Chapter 5 Working with the TitroLine alpha plus Input of reaction time titration parameter reaction time [s] waitung time in s: 0s 01 • • Following pretitration. the address of the burette(s) and the desired predosing volume have to be specified here.100 ml). TITRONIC® 200. If no predosing or pretitrating is performed. both waiting times will be added up. 286 The predosing burettes which can be used include: Piston burettes of the TITRONIC® universal. the address of the burette and the predosing volume are to be entered. and TITRONIC® 110 plus types . titration parameter pretitration 1 adress burette: 00 predosing in ml: 0. reverse titration with thiosulfate in iodometry. how many burettes are to be used. for instance. Up to three different predosing burettes can be used in one method. At this point. and if yes. The typical applications include. TITRONIC® 110. 078 Predosing titration parameter predosing no 1 burette 2 burettes 3 burettes 01 • At this point you specify whether predosing is to be done at all.01 . 072 If predosing is to be done using one or more burettes. or sample preparation with pH value setting using buffers.01ml 01 • If predosing is to be done (0. The first waiting time will run after predosing. a waiting time will run to ensure complete reaction.

The value can be input in terms of µl/min. Go on with method: Exit the input menu.gefüllt werden. this can save time! PLEASE NOTE: The burette can be filled at any time by pressing the unlatch button or the F4/FILL key. Min. In the latter case the solvent components contain a serial of constituents. End criteria: The end criteria consist of a current value in terms of µA with a specified cut-off time and/or a drift value in ml/min. However./max. Proceed with methods parameterisation. • 251 The input of the parameters for a µA titration is done in analogy with the parameterisation of a pH/mV titration. This kind of titration is subject to a given regulation which will be influenced by the titration leap to be expected and by the drift. time: Depending on the sample to be analysed. should this be desired. Drift stop: Setting of a drift stop. Polarisation voltage: Setting of the voltage between the two pins of the double platinum electrode. In the case of small titration volumes and automation using the sample changer. Karl Fischer titration type titration parameter KF settings type of reagent polarisation volt extraction time end criteria drift stop min/max time go on with method 01 • Reagent type: In the case of KF titration. irrespective of the change of the measurement signal. a distinction is made between a single-and a two-component reagent. which means titration will be finished as soon as the µA value which was input as end point will be reached. In the case of single-component reagents. neither at the beginning. only titration to one end point is possible. this is the item to enter the minimum or maximum titration duration. Extraction time: Setting of the waiting time which is required to either dissolve the sample or to extract the water from an insoluble sample. Linear: Titration is performed at constant dosing steps. all the reagent components required for titration are contained in the titration agent. nor at the end of titration.121 Chapter 5 Working with the TitroLine alpha plus Automatic filling of the burette titration parameter fill dosing unit fill do not fill 01 • • Filling: An automatic filling process takes place at the end of a titration Do not fill: No filling is performed. 105 µA titration type titration parameter uA titrations one end point linear (EQ) 01 • One end point: Titration will be carried out to ONE predefined end point. This means that reaction speed is somewhat slower than in the case of a two-component reagent. • • 217 • • • • .

in combination with the µA criterion. however. either to dissolve the sample or to extract the water from an insoluble sample (therefore the description "extraction time". 282 . may also be used for adapting to different solvents. a certain period of time has to be waited before the titration unit will start with the addition of the KF reagent.122 Chapter 5 Working with the TitroLine alpha plus titration parameter KF settings one comp. reagent.) The extraction time can be set in a range from 0 to 9999 s. In principle. For safety reasons. The selection of two-component control when using a singlecomponent reagent will be too slow. The current strength readings change as a function of the voltage being applied. two-component control is the default standard control method. the following is true: Higher voltage = Slower titration! 281 titration parameter KF settings extraction time 0s 01 • Extraction time: After the sample has been added into the titration vessel. If other solvents are being used. This waiting time is necessary. reagent 01 • • The control of a single-component reagent is based on the assumption of a slower reaction time. the selection of single-component control will lead to clear over-titration. the titrations may be optimised by modifying the polarisation voltage. 10-200 mV may be set. The standard set value is 100 mV. may be modified according to specific requirements. two comp. If a two-component reagent is being used. This value. 218 titration parameter KF settings polarisation volt 100mV 01 • Polarisation voltage refers to the voltage being applied between the two platinum pins of the double platinum electrode. This value.

123 Chapter 5 Working with the TitroLine alpha plus titration parameter KF settings end criteria cutoff curren 20 uA cutoff time 10 s 01 • • End criteria: The end criteria decide when titration will stop. the cut-off time resumes at 0. titration will stop. titration duration is a very suitable parameter for samples out of which the water is extracted only very slowly. The drift can be used very beneficially as a criterion for cancellation. The term of 'drift' refers to the reagent consumption over time. The standard set value is 10 seconds. in terms of µA. time: The max. The max. the cut-off time starts to run. titration duration is used on samples which create an increased drift towards the end. • 219 titration parameter KF driftstop no [µl/min] [ug/min] 01 • Drift stop: Some humidity will penetrate from the environment into each titration vessel. 220 . Max. caused by a slow extraction of water. It is indicated in terms of µl/min. even if it seems to be very closely sealed. In the case of such samples it makes sense to have the water reacted away by the addition of KF reagent as early as during extraction. Its standard setting for all methods is 20 µA. titration duration can be set from 0 to 9999 s. If the value drops back below the set value again. The drift will be displayed automatically during conditioning and in the course of titration. If the set cut-off current is exceeded for a period exceeding the cut-off time. The min. In this case the µl value will be multiplied by the titer. 221 titration parameter KF settings min/max time min time max time 01 • 1s 999s • Min. can be set variably between 1 and 100 µA. The standard value for all samples is set to 1 s. This means that the water will be extracted more quickly out of the sample. for instance. Cut-off current: The cut-off current. This humidity will consume KF reagent. The standard value for all samples is set to 9999 s. Cut-off time: As soon as the set cut-off current has been reached. [µg/min] means a higher value. and as a rule this value does not require any change. time: Min. titration duration can be set from 0 to 9999 s. and where no stable end points will occur.

115 . • 112 titration parameter set pH pH= 7. • • • • • Unbuffered: There is a strong rise. a pH-value (set value) will be kept stable titration parameter pHStat set phase unbuffered strong buffered flat jump medium jump steep jump 01 The set phase of titration corresponds to an end point titration.00pH Max. titration will be interrupted. The addition will slow down automatically when the set value is being approached. Flat jump: Example acetic acid with NH4OH (ammonium hydroxide) Medium jump: Phosphate. for instance with hydrochloric acid Strong buffered: The titration curve contains a flat section. titration parameter add reagent no automatic manual 01 • • None: No further substance is to be added. The titration parameters are set analogously. and a manual addition can be made.: 100 ml/min 01 Input of a pH value which is to be preserved during titration. titration will be stopped. for instance in the case of citric acid. This means that after the first reaching of the set value (exceeding 2 s). the following screen will be displayed and ask for an addition. first jump Steep jump: Strong acid with strong base 109 pH-stat unbuffered After selecting the titration parameters for pH-stat titration. the volume to be added and the address of the addition burette have to be entered.124 Chapter 5 Working with the TitroLine alpha plus pH-stat titration type In the case of pH-stat titration. then titration continues automatically. Automatic: The corresponding volume of a reagent will be added by dosing after the set value has been reached. and a manual addition can be made. Manual: As soon as the set value is reached. The process of value preservation will start immediately. It would be 10 ml/min in the case of a 5 ml interchangeable unit. The max. If 'automatic' is selected. dosing or titrating speed is: 100 ml/min with a 50 ml interchangeable unit.

a specific pH value is to be kept constant for five hours. a sensible interval should be defined for the measurement-value "pairs" to be stored. 116 The window for entering the value is not shown here. Titration control and dosing are not affected by this time. titration parameter measuring interval second minute hour day 01 • Considering that the number of measurement values without data reduction is limited. 118 . • 401 Subsequently. titration parameter duration total time second minute hour day month manual cancellation 01 • Specify the total duration of pH-stat titration. Automatic: The direction will be recognised automatically. (The measurement value will also be kept constant within this interval. If. pH value. one minute might be a recommendable measurement interval. Manual cancellation is always appropriate if the consumption of pH-stat titration and the reaction time cannot be estimated. The term of 'measurement interval time' refers to the waiting time between two measurement reading recordings. and indication of time.125 Chapter 5 Working with the TitroLine alpha plus Selection of the titration direction titration parameter titration direction increasing decreasing automatic 01 • • Increasing: To be set in the case of base (NaOH) addition. the basis of the measurement interval time is to be specified. Decreasing: to be set in the case of acid addition (HCl). The next thing to be specified is the overall duration of titration.) The result will consist in 300 measurement value pairs including consumption. for example. Attention should be paid to the number of measurement values and a sensible specification of the measurement interval.

5 ml. This process is referred to as “set phase”. 2 ml. Medium: Applications up to approx. This volume can then be added to the titration volume. • • • Very low: Applications in the range of up to 0. the pH value to be kept constant must first be set.5 ml • Low: Applications up to approx. Very high: Particularly high consumption. 20 ml.126 pH-Stat pretitration Chapter 5 Working with the TitroLine alpha plus titration parameter amount set phase add volume not used 01 • Frequently. High: Applications up to approx. It may also be taken into account in the calculation of the slope and consumption. 120 titration parameter pHStat [ml/min] very low low medium high very high 01 pH-stat titration allows titration with a firmly set pH value over a fixed period of time. The titration parameters depend on the added reagent volume and the speed of this addition. • 121 .

127
5.3.2

Chapter 5 Working with the TitroLine alpha plus Calculation menu item

At this point, you can perform calculations or edit the calculation parameters of the currently selected methods. In addition, this menu can be used to select, create, or modify formulae.

calculations

01

• •

New calculation: The result will be calculated once again. Formula selection EQ: Used for the calculation of one of several turning points Formula selection EP: Used for the calculation of one or several end points (EP) Formula selection KF: Calculation of the result in the case of KF titrationFormula selection pHst. : Calculation of the result in the case of pH Stat titration

formula formula formula formula

select select select select

EQ EP KF pHst.

• •

106 Formula selection, calculate number of EQs

calculation calculate EQ(s) enter no. of EQ’s 1

01

Here the numbers of inflection points are set.

442 Formula selection EQ/EP

calculation calculate EQ(s) EQ 1 EQ 2 EQ 3 EQ 4 EQ 5

01

At this point you can select the formulae for the calculation of the respective inflection point (EQ). There are 5 EQ’s and 3 EP’s possible. The selection of the formulae for the end points is done analogously.

390

128

Chapter 5 Working with the TitroLine alpha plus

calculations formula selection mL*F1*F2/Q (mL-B)*F1*F2/Q (mL1-mL2)*F1*F2/Q (B-mL)*F1*F2/Q Q*F1/((mL-B)*F2 (B*F3-mL*F1)*F2/Q

01

At this point you can select the formulae for the calculation of the respective turning point (EQ) or end point (EP). You may select one out of the following formulae: mL*F1*F2/Q: Normal formula (mL-B)*F1*F2/Q: Normal formula with consideration of blank value (mL1-mL2)*F1*F2/Q: Difference between two EQs or EPs (B-mL)*F1*F2/Q: Reverse titration Q*F1/((mL-B)*F2: Titer determination (B*F3-mL*F1)*F2/Q: Reverse titration with additional calculation factors

• • • • • • 141

PLEASE NOTE: The formula for the <(mL1-mL2)*F1’F2/Q> difference formation must only be used from the use of a minimum of 2 EPs or EQs.

Formula selection KF

calculations formula select KF (ml-B)*Tit*F1*F2/EW EW*F1*F2/ml-B*F3 ml*F1*F2*F3

01

• • • •

At this point you can select the formulae for the calculation of a KF titration. (ml-B)*Tit*F1*F2/EW: EW*F1*F2/ml-B*F3: ml*F1*F2*F3:

404 Formula selection pH-stat

calculations formula select pHSt mL*F1*F2/Q (mL-B)*F1*F2/Q (Stg*F1*F2)/Q

01

• • • •

At this point you can select the formulae for the calculation of a pH-stat titration. mL*F1*F2/Q (mL-B)*F1*F2/Q (Stg*F1*F2)/Q

405

129

Chapter 5 Working with the TitroLine alpha plus

Legend of the abbreviations used in the formulae: = The calculation uses the ml values as determined, i.e. also ml 1, ml 2 etc. = Factor 1 (multiplicative) = Factor 2 (multiplicative) = Factor 3 (multiplicative) = Quotient; will be multiplied automatically with the respective sample weight or sample volume in the calculation Tit = Titer of the titration solution B = Blank value (additive) Stg = Slope in the case of pH stat The following menu items can be used to enter the desired values. ml F1 F2 F3 Q

calculations enter F1 enter value 1.00000 from global memory

01

• •

The factor may be any numeric value, for instance the molecular weight or molarity of the titration solution. It may also be taken from a global memory. This makes sense, for instance, in the case of a titre determination with a separate method.

339

calculations enter Q enter value 1.0000 from global memory

01

Normally, the quotient is located under the fraction bar. It serves first of all as a multiplicative factor used in the conversion of the sample weight. The sample weight is made in [g], but the calculation frequently requires [mg]. In the case of titer determination, the quotient is above the fraction bar.

342

calculations enter B enter value 0.00 from global memory

01

This item can be used to enter a value for the blank value. As an alternative, the value may be taken from a global memory which was calculated or determined in a different place (a different method). The value is used in an additive form.

146

130

Chapter 5 Working with the TitroLine alpha plus

The result consists of an identifier, a calculated value, and a unit (for instance %).

calculations enter unit unit

01

At this point, the unit may be entered (max. 11 characters).

344

calculations enter identifier identifier

01

At this point, the identifier may be input, for instance chloride (max. 11 characters).

345 On this menu you can select whether the calculation of the titration is to be done with arithmetically smoothed or "raw data".

calculations smoothing no smoothing weak smoothing medium smoothing strong smoothing

01

• •

No smoothing: Raw data will be used. Weak/medium/strong smoothing: The degree of smoothing is sets by an arithmetic algorithm in such a way that smoothing with different strengths will occur. In principle, only the raw data will be stored in the devices. Smoothing is done only in the calculation. The smaller the degree of smoothing, the more accurate will the results be.

149

131 Chapter 5 Working with the TitroLine alpha plus calculations no. Delete statistics calculations statistics delete manual delete automated 01 If the statistics feature was activated. decimals 1 01 • This menu item is used to enter the number of decimal digits of the result or the results. decimals no. 284 . only the last calculated value will be stored. a printer or PC has to be connected. the statistics memory will be deleted automatically. • 150 IMPORTANT: To document the statistics. but also in a different method If more than 1 EQ or EP have been calculated. A subsequent documentation of the individual values is no longer possible. the statistics memory may be deleted at this point. • • Manual deletion: The statistics memory will be deleted. respectively. This value can be used in a different formula. Automatic deletion: When a multiple determination is started. 103 calculations result into memory not into memory into global memory with number 1 01 • Here you can store the result of the calculation.

101 5.. 01 • If more than one EQ was selected. selection end selection standard deviation 335 calculations go back formula formula formula formula proceed to select select select select EQ EP KF pHSt.). IMPORTANT: Standard deviation has to be selected to activate full statistics processing..132 calculations Chapter 5 Working with the TitroLine alpha plus 01 • • Selection end: You leave the calculation menu and return to the method creation selection. calculations formula selection own formula 01 • • Within a method either predefined formulas or own formulas can only be used.3. They are selected in the methods from a list 181 .3 TL alpha plus formulae editor You can use the formula editor to create formulae of your and integrate them into methods. this is the point to select formulae and enter factors for further EQs. Since many formulas contain the same numerical values (for example chloride in cheese. water. these formulas can be predefined.

133 calculations Chapter 5 Working with the TitroLine alpha plus 01 • own formula list formula editor print list export list In this example the formula in formula editor was already created for another method and has to be selected only from a list in this case. of EP’s/EQ’s 1 01 • The number of the formulas must be entered in this method and the number of the EQ´s or EP´s used in these formulas. of formula’s 1 no. 152 calculations formula list result: not into memory into global memory with number 1 01 • It is decided here whether a result is supposed to be set for other methods and formulas availably. The result can be stored in one of 50 Global variables 155 . 153 calculations own formula no.

160 . 159 calculations enter identifier identifier 01 • Here the result name can be entered. for example chloride (at the most 11 signs).134 Chapter 5 Working with the TitroLine alpha plus calculations enter unit unit 01 • Here the unit can be entered (at the most 11 signs).

decimals 0 01 • Here one gives the number of the decimal places of the result and/or the results. the result safety very restless titration curves. The smoothing occurs only during the calculation. however. 103 calculations smoothing no smoothing weak smoothing medium smoothing strong smoothing 01 • • Do not smooth: Is worked with raw data. Only the raw data are stored fundamentally in the device. the more precise the results are. Weak Smoothing / Average-Strong Smoothing / Strong Smoothing: The degree of smoothness is set by a mathematical algorithm so that a smoothing is received in different strengths. The smaller the degree of smoothness is. CAUTION: In the case of standard deviation selection the statistic is activated. It can improve. Normally a smoothing is not necessary. decimals no. 335 . 149 calculations selection end selection standard deviation 01 • • End selection: The calculation menu is left and goes back to the selection method creation. By means of the first derivation of the titration graphics a judgement is slightly possible.135 Chapter 5 Working with the TitroLine alpha plus calculations no.

Five EQs from 1 to 5 are possible. 161 . accompanied by a blank. a decimal point. A formula used to calculate chloride in terms of % would then look as follows: EQ1 * 35.1 *100 / (1000 * W) The equivalence point is a variable and referred to as EQ1. 1 formula name Fo-----------1 overwrite 01 • 170 calculations enter formula EQ1 * 35.1 * 100 / (1000 * W) 01 - The formula is input like normal text using the keyboard. 153 calculations formula editor no. The other arithmetic factors (e. The molecular weight ”35. “0. The operators include the asterisk (“*”) for multiplication and the slash (“/”) for division.45 * 0.136 Chapter 5 Working with the TitroLine alpha plus Create an formula with the editor: calculations own formula formula list formula editor print list export list 01 • The “Formula editor” selection will take you to the menu for creating own formulae.1” for the molarity and “100” for the conversion to % and “1000” for the conversion of G to mg) are to be typed.g.45 * 0.4”5 is input in the form of a number incl.

-. EP3 End points EV End volume. 169181 calculations own formula no.137 calculations Chapter 5 Working with the TitroLine alpha plus 01 - formula editor formula selection new formula print formula formula exporting The formula which was just created may be printed or output via the RS interface. / (). At this point it is also possible to select the formula right away to integrate it into the method.456 Numeric value Editing: ▪ Negative values to be entered between brackets ▪ Del key: The character at the cursor position will be deleted ▪ Backspace: The character to the left will be deleted ▪ Arrow keys: Direct cursor movement to desired position ▪ Number of characters: 140 characters on 8 lines . of formula’s 1 no. consumption at the end of titration W Weighed-in quantity V Volume pattern M1 .. (-1) Arithmetic operators EQ1 . of EP’s/EQ’s 1 01 - The input to be made here includes the number of formulae included in this method and the number of EPs or EQs to be used within these formulae. M50 Global memories (ln Zahl) Natural logarithm to the base of e (lg Zahl) Logarithm to the base of 10 (ex Zahl) Exponent to the base of e (sq Zahl) Square root 123. EQ5 Equivalence points EP1 . *... 152181 Functions +.

GLP documentation shortlist 01 • No documentation: The results will not be output on the printer. date. Standard documentation: In addition to the short documentation. the device can be used for measuring.1 NaOH mol/l concentration 1000 Conversion ml to litres V Pattern in ml (sample quantity) Chloride % EQ1 * 35.04 * 0. and result. shortlist • • • • 107 • .138 Chapter 5 Working with the TitroLine alpha plus Input: Directly at cursor position Examples:: Citric acid mg/litre EP1 * 64. the titration chart will be printed.3. In this way. Short documentation: Output of the method name. dosing. including the method parameter. detail documetation: Besides to the standard documentation the formula with all values is printed GLP documentation: All attitudes are printed.04 equivalent weight of citric acid 0. for instance. time. and setting of the pH value without wasting paper.45 * 0.45 Mol weight of chloride 0.1 * 100 / ( 1000 * W) EQ1 Equivalence point 35.1 AgNO3 concentration 100 Conversion to % 1000 Conversion g to mg W Weighed-in quantity in g 5.4 Documentation menu item titration parameter documentation no documentation short documentation std documentation detail documentat.1 * 1000 / V EP1 End point 64.

1 print meth. print list: A list containing names and numbers of the working methods. Subsequently.5 Chapter 5 Working with the TitroLine alpha plus Method management This menu item can be used to administer. Print method 1: All parameters of the selected method will be printed on the connected printer. the current method number is displayed as the one to be deleted. method management meth. print. 1 copy meth. edit. the selected method will be deleted without security prompt. Print list: A lists containing the numbers and names of the working methods will be printed.meth. it can be overwritten by the desired number. The current method can be stored under a new number. export list: During export. pH titrations mV titrations uA titrations KF titrations pH-Stat 01 • This item is used to preselect the titration type of the standard method. However. etc.139 5.* *Not in version 1. this list will be output in ASCII via the RS-1 interface • • • 004 • • • Standard methods atandard methods selection print list export list 01 • • • Selection: This item is used to select the standard methods of the respective titration modes.3. this new method can be modified in a time-saving manner. 1 delete meth. When confirming the selection with <enter> or < ↵ >. Export method: The term of ‘export’ refers to its output via the RS interface 1 in the form of a text file. a method can be selected or edited from a selection of standard methods. Standard methods: At this point. 037 .0 036 standard methods selection std.* export list: If exported. this list will be output through the RS 1 interface in ASCII form. existing methods. 1 export standard methods print list export list 01 • Delete method 1: Firstly.

To correct erroneous input. Methode 1 01 • Here you may entered the number and the name of the new method to be created. If password protection is active you will first be prompted to enter the password. you may select the type of pH titration. for instance end point acids. Below. If you press the <ENTER> or < ↵ > key. you will be taken to the selection (032) dialogue window.140 Chapter 5 Working with the TitroLine alpha plus Example: pH titration type standard methods selection pH end point acids EQ acids org Solv acids end point bases EQ bases org Solv bases 01 • At this point. (please refer to chapter 5. press the < ← Backspace> key. This window can be used to program all desired parameters for the new method. • 243 . please enter the desired method number under which the standard method is to be stored. you may select a different standard method.6 Create new method With this menu item you can create a completely new method according to specific requirements. pressing <ENTER> or < ↵ >will take you to the following menu item: new method method number 1 enter method name. If the password protection is off.3. 038 standard methods method Citric acid store as number of method 1 01 • • The selected standard method will appear under 'method'.3. 045 5. page 21). Using the ↑ and ↓ keys.1.

French. and in this case its temperature reading will be used. Presently not yet active! Temperature: The temperature for calibration is to be set manually here. the setting of the calibration data is completed by “End of selection”. English.00 and 7. The march of temperature of the buffer may be input from 10°C to 40°C in steps of 5°C. as a rule 4. All buffers once selected. system settings system settings language calibration setting dos speed/filltime delete all methods user hardware 01 • • • • • Language: You may select between German. The buffers are selected in a row (please refer to fig. date and time. you may select up to two buffers out of a set of standard buffers and freely definable buffers to be used for pH calibration. Calibration setting: This item is used to define which buffers are to be used for calibration. They are provided with calibration data which can then be documented together with the name of the sensor in a form which is suitable for FDA/GLP.141 5. • 018 . etc. A Pt 1000 will be recognised automatically. Hardware: Settings required for RS parameters.00 °C new buffer sensor management Buffer selection: At this point.! Sensor management: This item is to be used to attribute names to sensors (electrodes). 007 Calibration setting system settings 01 • calibration setting buffer selection temperature: 25. the general device-specific settings for the TitroLine alpha plus Titration Unit are made. and Spanish.3. We recommend calibration using two buffers. a pH end point titration is only possible with a valid and correct calibration. Delete all methods: All user methods will be deleted. Presently not yet active! Validation: If validation is active. Calibration can be carried out with 1 to 3 buffers.00. such as language. 094). Dosing speed/filling time: Setting of the dosing speed and filling time. password protection. if no Pt 1000 is connected. printer selection etc. Presently not yet active! • • • 017 • Buffer selection calibration setting buffer selection calibration 2 point selection buffer 1 selection buffer 2 selection buffer 3 end selection 01 • This item is used to define the buffers for calibration. New buffer: To be used for the definition of own buffers.7 Chapter 5 Working with the TitroLine alpha plus System settings At this point.

6. Automatic: If a temperature sensor (Pt 1000) is used.0 . • 094 Temperature compensation calibration setting temperature compens manual automatic 01 • Manual: Setting of the temperature at which the titration unit will work in the case of a pH measurement without temperature sensor.00pH 01 • • New buffer: Specification of the number. for instance: 2. or if a special buffer is required.0 4.7.18 .0) which is provided either by SCHOTT Instruments. it may be defined at this point. Value: Input of the value.10.45. Each buffer can be selected from a list (buffer pH 1. Own buffer: At this point you can select buffers which were previously self-defined by the user inclusive of their temperature march. temperature compensation will be performed automatically.4.01 . 024 .9.142 Chapter 5 Working with the TitroLine alpha plus calibration setting selection buffer 1 standard buffer own buffer 01 • Standard buffer: Ex-works predefined buffer. or by the device manufacturer for the specific application case. • 022 New buffer If the default standard buffers should not be sufficient. The default setting is 25°C.87 . calibration setting new buffer new buffer 1 value 7.0 .

00pH 20° 7. but please note the following: • To enable the TitroLine alpha plus Titration Unit to recognise that a new buffer is being recorded. • • 025 Sensor management calibrate init.143 Chapter 5 Working with the TitroLine alpha plus calibration setting deviation/degree : 10° 7. In addition.00pH 25° 7. sensor management new sensor delete sensor print list export list 01 • • • • • new sensor: Used to activated a new sensor.00pH 15° 7. After pressing the <ENTER> key. the pH values above and below the later measurement temperature at which calibration is to be performed have to be entered. export list: The exported list contains the same information as the printouts and will be output in the form of a text file via the RS 1 interface 129 . sensor management yes no 01 • • Yes: At this point you may store the Information regarding the sensor be used.00pH 01 It is not necessary to enter all of the pH values of the buffers. Example: The calibration temperature is around 15°C. This means that the 10°C. 15°C and 20°C as well as the 25°C pH value of the buffer have to be input. for instance an N 62 glass electrode. the 25 °C value of the buffer has to be entered. you may input the next four values in the temperature range from 30 to 45 °C. print list: Used to create a printout of the list of the currently defined sensors including the calibration data. No: Proceed with the inquiry of validation information 126 calibrate init. delete sensor: Here you may select and delete a sensor.

possible value (100 ml/min) Filling time max.144 Chapter 5 Working with the TitroLine alpha plus sensor management new sensor sensor 1 sensor name Charge Nr.g. speed = 100 ml/min. 12345 130 sensor management Sensor pH mV uA °C 1 01 This is the point where you specify the type of the sensor. the filling time can be extended between 20 s to 999 s. 01 • • At this point you can input a name for the sensor.: The dosing speed is set to be max. possible value. (20 s) Set filling time: For samples with a higher viscosity. Set dosing speed: The dosing speed may be adapted to the application in a range between 0.: The filling speed is set to the max. e.01 ml/min to up 100 ml/min. shortest filling time = 20 s • 013 . N 62 no. dosing speed max. for instance.00ml/min 20 s 01 • • • Dosing speed max. 190 Dosing/Filling dosing dosing speed. filling time max. Default setting: max. set filling time set dosing speed back 100.

the filling time has to be adapted. The possible filling times stretch from 20 s / 100% filling volume and 999 s/100% filling volume. the piston remains in any position. a reduction of the filling speed to 20 s / 100% filling volume should be sufficient. The standard setting is “always” 035 dosing speed dosing speed ml/min 100. • 034 . Always: Automatic filling will take place at the end of each titration After titration: Only after a titration is filled automatically. adjustable dosing speed per minute corresponds to double the dosing volume of the dosing unit being used.145 Chapter 5 Working with the TitroLine alpha plus The menu items below are used to specify the desired filling time. As a rule. After dosing: Only after dosing it is filled automatically. it applies to all methods. Please note! The set filling time applies irrespectively of the selected method. it applies to all methods.e. i. filling time 01 • input via keyboard 20s • If the liquid to be dosed has a too high viscosity or a too high vapour pressure. dosing speeds from 0. i. 033 fill automated always after titration after dosing never 01 • • • • • This menu is used to select whether and when the burette is to be filled after the end of a titration. speed is defaulted.e. for instance: 100 ml/min with a 50 ml dosing unit.01 ml/min up to 100 ml/min may be input. Depending on the dosing unit. and the burette will only be filled with the start of the next titration. The max. Please note! The set dosing time applies irrespectively of the selected method. Never: Upon the end of titration.00ml/min 01 • The max.

the system will dose. Check.146 Chapter 5 Working with the TitroLine alpha plus Dosing speed / Filling time: To get to the “Rinsing/Dosing” window. If the device contains one and the same reagents and is not used over a certain period of time. Set dosing: The volumes may be selected or preset. (please refer to Technical Data) 011 . rinsing/dosing rinsing dosing set rinsing set dosing 01 • • • • Rinsing: After pressing the ENTER key. please press <ESC> on the main menu. 010 dosing set dosing input via keyboard 1 ml 2 ml 5 ml 10 ml 20 ml 50 ml 01 • • Input using the keyboard also enables the input of odd volumes. two rinsing cycles are performed in the course of initial filling. such as 1. Rinse two times: Two rinsing cycles will be carried out. Rinse three times: Three rinsing cycles will be carried out. single rinsing may frequently be sufficient. Set rinsing: specification of the number of rinsing cycles. The default values are available for making a selection without using an external keyboard. the system will rinse. You may also use the R/D key on the built-in keyboard. Dosing: After pressing the ENTER key. if defined volume can be dosed with the actual dosing unit in required accuracy.00 ml. 005 Set rinsing set rinsing rinse once rinse two times rinse three times 01 • • • • Rinse once: One rinsing cycle will be carried out. Maximum 3 rinsing cycles. Normally. Please note: Some volumes are predefined for working without an external keyboard.234 ml. The standard setting for dosing is 0.

Each device requires a different “address” (or number). stop 1. 1. no. subsequently. parity no no odd odd even even 01 • • Selection of the possible combinations for the settings required for RS interface 1. 014 . Interface 1 is used for connecting a PC or a serial printer or for daisy-chain data transmission. Printer: Selection of the desired printer Sample changer: This menu is used for moving the sample changer manually. 1. and the method management. 8.147 Hardware settings Chapter 5 Working with the TitroLine alpha plus system settings hardware RS parameter date/time beeper password printer sampler 01 • • • • RS parameters: Configuration of the RS interface Date/Time: Setting of date and time Beeper: On/off of the acoustic signal Password: Possibility of entering a password in order to protect the method parameters. the second device is connected through its first RS interface to the second RS interface of the first device. the system settings. The term of “daisy chain” refers to a concept where the first device is connected through the first RS interface to the PC. 7. PLEASE NOTE: The parameters have to match those of the connected device. 1. Two RS interfaces are available. The default setting is 7. interface 2 is used for a weighing balance or for daisy-chain data transmission. 8. • • 178 RS-parameters RS parameter RS adress 01 01 • This item is used to select the RS interface. 8. 1. 1. 240 RS Com 1 word 7. 7. 1.

day. The units are not to be input. the corresponding menu is can only be called up using the password. 238 Date / Time date/time year 2000y 01 • Year. second are inquired one after another. PLEASE NOTE: The parameters have to match those of the connected device. 026 Password If desired. since they will be attached automatically. In case the current password should be lost. If required. hour. system settings password activated yes no 01 • Yes: A password consisting of min. 11 alphanumeric characters can be input. Afterwards. month.148 Chapter 5 Working with the TitroLine alpha plus RS Com 1 baud rate 2400 baud 4800 baud 9600 baud 19200 baud 01 • • Selection of the data transmission rate of the RS interface. you may obtain this "master password" from SCHOTT GERÄTE. 5 up to max. Each input has to be confirmed with <ENTER>. • 241 . No: The corresponding menus will not be protected. there is a "master password" which may be used to retrieve the lost one. minute. Method creation.. The default setting is 4800 baud. method management. and the system settings can only be called up if the user knows the suitable password. a password may be entered here.

TW head down: The titration head moves down.149 Chapter 5 Working with the TitroLine alpha plus system settings password enter password repeat password 01 • • A password has to have a minimum length of 5 and a maximum length of 11 characters. Examples: . TW reverse : The preceding sample will be approached. the appropriate printer may be selected. if the < > and the ENTER key are pressed. we recommend the HP low resolution mode. the password will be stored.HP Deskjet: Almost all HP printers with PCL III The selected driver will be activated. and the input has to be repeated.Epson ESC/Raster: Epson Stylus C 70 / C 80 .Epson ESC/P2: Epson Stylus Color 880 . 395 . 125 Printer system settings HP LowRes HP HiRes Epson LowRes Epson HiRes RS export 01 • At this point. stirrer off: filling bueretts: The burettes are filled. Please note: If the two passwords do not match. As soon as you press the < > and the ENTER key. Pressing the < > key will take you to the second input field. the result will be output through the RS interface in the form of a text file. TW advance: The next sample will be approached. • • 315 Sample changer (TW) sampler TW head up TW head down TW advance TW reverse stirrer off filling Bueretts 01 • • • • • • TW head up: The titration head moves up. the password protection is not in place.For reasons of speed. The first input field was selected. RS export: If the RS export setting is set.

measuring channel -0. µA: display of the µA value of a connected double platinum electrode. measure pH pH mV mV °C uA A B A B 01 • • pH A: Display of the pH value of a pH electrode on the A measurement input.000 pH 25.3. mV B: The mV value of the electrode on the B measurement input is shown in analogy to the pH value.150 5. °C: Display of the temperature.0 °C 300 . • • • 008 • Measurement pH A/B. pH B: Display of the pH value of a pH electrode on the B measurement input. example: B measurement channel measure 01 B • Display of the µA value of a pH electrode on B measurement input. mV A: Display of the mV value of an electrode on the A measurement input. both electrodes may be of the single-rod type with built-in reference electrode.e. i. Both measurement inputs are potentialcontrolled.8 Measurement Chapter 5 Working with the TitroLine alpha plus This item can be used to select the desired measurement range for the display of the desired measurement unit.

unit Print meas. 337 show calibr.151 5. value 01 • • • • • Print titration: The documentation of the last titration will be printed. Validated: Currently without any meaning.00 pH buffer 2 7. value: TEXT??????? 009 • show calibr. “Date/Time”. data show calibr. data print result list print meas.00 PH buffer 3 01 • • Display of the selected buffer and the manual temperature. “Method”. data validated: temperature 25.00 pH zero point: 7. Validation: Indication whether validation is active or not. The setting of the method will be used. Print cal data: The currently valid calibration data will be output. output print titration show titration print calibr.00 pH proceed 01 • Display of the slope % and the zero point of the electrode in term pH. Print result list: The calculated results of the last 24 samples will be printed in the following order: “Name of the sample”.3.via the printer interface. “Result.9 Output Chapter 5 Working with the TitroLine alpha plus This menu can be used to output various data . data sensor 1 slope: -59. 338 . value.00 °C buffer 1 4. Show cal data: The currently valid calibration data will be displayed. Show titration: The titration result will be shown on the display.

133 . 7.152 5. then start calibration with the <ENTER> key.4 Chapter 5 Working with the TitroLine alpha plus The Cal menu Calibration is called by pressing the <F8> key or < >. Calibration is defaulted to an automatic 2-point calibration with the 4. calibration sensor measuring channel measuring channel A B 01 • Selection of the measurement channel to be calibrated. 4. It is also possible to define buffers of your own. 9. it is possible to give an estimation as to the correctness of the current values.16.00. 132 For the second buffer a new invitation in the form of an acoustic signal will be issued after calibration.00.00. Considering that the theoretical mV value is pH 59.00 pH measvalue: -144.8 mV 25° 01 • The display will show the buffer value and the current mV value of the electrode.18 und 10. A distinction is made between channel A and B. 4.01.Set calibration” you may select two out of seven preset buffer solutions. Immerse the rinsed electrode into the buffer solution. The following DIN 9266 buffers and NBS buffers are available: 1.00.87.00 and 7.00 DIN buffers.00pH 01 • An acoustic signal will ask you to start calibration. press <ENTER> 131 calibration buffer 1 rinse electrode dip into buf.. Buffer 7. 6. To proceed. calibration buffer checking expected pH: 4. From “System settings .

2 % 01 • • If an incorrect steepness is detected in the course of calibration. To leave the calibration mode. With <ENTER> you confirm that the electrode is immersed. Please check your buffer solutions first. the zero point and the slope will be displayed.00pH 01 • The way of proceeding is analogous for the second buffer. With <ENTER> you confirm that the electrode is immersed.153 Chapter 5 Working with the TitroLine alpha plus calibration buffer 2 rinse electrode dip into buf. Buffer 4. . The following error messages may appear: calibration error slope slope is out of specification zero point: 7. 136 calibration error buffer buffer is wrong ⇐ 01 • The way of proceeding is analogous for the second buffer. this message will be output. please press the <ENTER> key at this point. then the electrode. 137 Upon the successful calibration of the electrode. pressed the <ENTER> key. 132 To leave the calibration mode.00 pH slope: 108.

154 Chapter 6 Method creation 6 6.1 Method creation .

155 Chapter 7 In/Output for data communication 7 In/Output for data communication KF Pt Electrode A Electrode B 1 RS-232-C 2 Keyboard Printer Centro- Ref Ref 230V:0.25 AT I 0 Stirrer 12V out 1 2 3 4 5 6 7 8 9 10 11 Electrode connector pH A/mV A and pH B/mV B Connector for reference electrode channel A and B Mains switch KF connector Connector for temperature-measurement sensor Mains connector Printer connector (Centronics) Stirrer connector (Stirrer) Connector for weighing balance (RS-232-C) Keyboard connector Computer connector (RS-232-C) .

Data 7 Acknlg Busy PE SLCT Pin no 14 15 16 17 18 . “Centronics / Printer”)). The device cannot be parameterised without an external keyboard being connected. non-aqueous solutions Redox. It is used to output result protocols. The TitroLine alpha plus Titration Unit is equipped with a Centronics interface (parallel).titrations Chlorid.1 Keyboard port The keyboard port is used to connect the TZ 2835 PC keyboard.2 7.titrations Temperaturemeasurement/ compensation Titration with ion-sensitive electrodes Reference Electrode N 6280 Connection Stecker Buchse an Elektrode am Gerät A (DIN)/BNC A or B Electrode cable K1A/ K1BNC N 6480 LiCl/Eth.25 Meaning NC ERROR Init GND GND . item 7. Pin configuration of the Centronics interface: Pin no 1 2–9 10 11 12 13 Meaning Strobe Data 0 .156 Chapter 7 In/Output for data communication Typical applications with available electrode and piston options Application PH titration. on a printer with the same interface. The plug-in port for this interface is located on the back panel (figure 6.“ K1A B1A 7. 7. Pt 6280 AgCl 62P7 TZ 1106 NN (4mm „banana“ type) W 2130 NN (4mm „banana“ type) TZ 1101 A (DIN) TZ1103 TZ 1104 B 2920 N „banana“ type „KF“ „Pt 1000“ K1NN 9907/21 A or B „Ref.titrations Karl-Fischer. aqueous solutions PH titration. charts etc.3 Power connector Centronics-Interface Please ensure that the mains voltages matches the voltage set at the bottom panel of the device.

In this case. T x D data input Digital Mass n .4 Chapter 7 In/Output for data communication RS-232-C-Interfaces The TitroLine alpha plus Titration Unit is equipped with two serial interfaces (RS-232-C) for data communication with other devices. The ex-works settings of the transmission parameters are: 4800 baud. n. the two middle keys of the TitroLine alpha plus Titration Unit have to be pressed simultaneously with the device being switched on. This will initiate an internal data reset which is indispensable in view of the device’s proper functioning. 7 bit. It is also possible to update the standard methods using the RS interface. 19200 The remaining parameters are as follows: Data bits Stop Parity 7 1 no 8 1 no 7 1 odd 8 1 odd 7 1 even 8 1 even 7.5 Loading the operating-system software via the RS-232-C interface The TitroLine alpha plus Titration Unit is equipped with a FLASH memory for storing the OS software. 4800. you need a PC with a free serial interface. This is a convenient feature if enhancements and improvements of the functions of the TitroLine alpha plus Titration Unit lead to the generation of a new software version. Optional parameters: Baudrate 1200. c. the download software from SCHOTT. . To use this feature. 2400. too. the required data are to be downloaded into the device through the RS interface. c.c n .c Pin no 2 4 6 8 Meaning R x D data output n. The transfer parameters of all devices communicating with each other have to be the same. please refer to the operating instructions of the download software. Pin no 1 3 5 7 9 Meaning n. The “Balance” socket can be used to connect a weighing-balance. 9600. no parity. This FLASH memory enables new software versions to be loaded down via the RS 232 C interface from a PC onto the TitroLine alpha plus Titration Unit. The “Computer” socket is used to establish the connection with a computer or printer with a serial interface. c. For the way of proceeding. and a serial data cable of the TZ 1581 type. IMPORTANT NOTE: After the download of the software.157 7. 2 stop bits. CTS Please note: The data-transmission parameters are to be set in the “Systems settings” and <<RS>> menu. The plug-in ports of these interfaces are located on the back panel of the device.

with volume addition aaGF30 aaY Filling time in seconds (30 s min. All replies will be returned to the computer after each action has been completed.080 ml an on-line measurement value aaQE aaY Stirrer on aaQA aaY Stirrer off aaLC Output of calibration values via RS computer aaLD Output of datav aaBF aaY Burette is filling aaDB01.6 Chapter 7 In/Output for data communication Controlling the TitroLine alpha plus Titration Unit via the RS-232-C interface The following commands can be used to control the TitroLine alpha plus Titration Unit via the RS-232-C / Computer interface: <aa> steht für die Adresse des jeweiligen Gerätes (immer zweistellig).3 aaY Dosing rate in ml/h aaGDM20.158 7.912 pH “Online measurement”.12 aaY Burette is dosing volume without filling and zero-setting.08 aaY Method change aaFD aaY ‘Measure dead stop’ function aaFPA+B aaY ‘Measure pH A + B’ function aaFVA+B aaY ‘Measure mV A + B’ function aaFT aaY ‘Measure temperature’ function aaM Measurement value Measure within the set measuring range aaO OOM:1. Command aaVE aaRH aaRS ..12 aaY Burette is dosing volume without filling. output of 0.) aaGDH 11. Reply Action Version: 060602-01 Output of version number Ident: TL alpha plus Hardware report Status: READY/MODE/INIT/CAL Report status (where am I?) aaRC Last command Repeat last command aaRA aa UNIT: 10 ml Inquiry of unit size aaSM aaY Start of a method aaMC01.3 aaY Dosing rate in ml/min Each command must be entered separately and concluded with <CR> <LF>. aaDA01. Please note: Commands involving small volumes which cannot be dosed will not be executed.

and leaks. For this reason. the maintenance intervals are to be reduced accordingly.nominal/actual − − − Function: Karl Fischer titration (dead-stop) Interface functions.2 Maintenance and care of the burette section Proper functioning of the TitroLine alpha plus Titration Unit has to be maintained by check and maintenance work. g. measuring amplifier. the electrical contacts have to be checked if the titration unit is used in premises with an occasional occurrence of corrosive matters in their atmosphere. an error. alkaline. Defective glass parts must always be replaced. control behaviour. This maintenance or service work include: − Visual control: − Measurement ranges mV: pH: °C: Front-membrane display 3 values . has to be carried out. Once per quarter. Part 6. keyboard function. the dosing accuracy of the device is to be verified. and in addition a test according to DIN EN ISO 8655. Under particular stress: − Once per quarter. and seals for signs of visible damage. this means.1 Maintenance and care of the TitroLine alpha plus Titration Unit Maintenance and care of the titrator section Proper functioning of the device has to be maintained by check and maintenance work. the glass cylinder has to be inspected visually in order to check for damages. that the glass surface may be damaged by the use of chemicals. These inspections are particularly necessary if solutions were used which are likely to attack glass. contamination. printer output. . the valve and the glass cylinder are parts which are subject to wear and tear. Check the hoses. If there is any suspicion that a solution is attacking the glass excessively. such as cracks. fractures and damage of the surface. screw unions. After each maintenance operation. Defective parts must be repaired or replaced by new ones. a test for metrological reliability according to DIN EN ISO 8655. has to be carried out. fluoride. e. stirrer connection. or phosphatic solutions. Maintenance work to be carried out − − − − − − Check the piston and the cylinder for visible signs of mechanical damage. This wear and tear may lead to inaccurately dosed volumes.159 Chapter 8 Maintenance and care of the TitroLine alpha plus Titration unit 8 8. As a rule.nominal/actual 6 values . intervals for carrying out all maintenance work is 6 months. − In case of disturbances: The work has to be carried out immediately if a disturbance. Check the electrical plug contacts for corrosion and mechanical damage (on the titration unit and on the cables).nominal/actual 3 values . Part 6. the max. 8. dialog system. Maintenance intervals Normal operation: In a larger sense. or another defect becomes obvious. Check the piston rod for corrosion.

or to be dislocated. In this regard. have to be drained. If the liquid is left in the system. Reproducibility of results − The measurement results or analysis results depend on a variety of factors. Please note: The main printed board contains 2 lithium batteries. In no case must water penetrate into the interior of the lower section. Since the state of the art does not provide plastic hoses which are absolutely free of diffusion occurrences. in many cases this packing will not be available any more.160 Chapter 8 Maintenance and care of the TitroLine alpha plus Titration unit Periods without operation − We If the dosing units for the TitroLine alpha plus Titration Unit are not used over a long period of time. the liquids contained in the system. The devices should be stored in a room with a temperature between +10 and +40°C. However. please contact SCHOTT Instruments. or rinsing with. but this method should only be applied in extreme cases. so that one will have to compose an equivalent packaging system. short-term immersion in. Please check the plausibility of the measurement results or analysis results at regular intervals. please adhere to the usual validation procedures. the liquids contained within the system. water or ethanol is not harmful. Sealing the lower section in a foil is hereby recommended. 10 Recycling and disposal The present titration unit and its packing material were mainly made from materials which can be disposed of and recycled in an environmentally friendly manner. these precaution measures apply in particular to the hose-line section. and the (relative) humidity of the air should not exceed 70 %. using the original packing will be the best protection of the device. one has to reckon with corrosion and an alteration of solutions used over the time. in particular aggressive solutions. The casing of the lower section may also be cleaned using a piece of cloth and a household cleansing agent. In general. If dosing units are to be stored over some time. “Maintenance and care of the burette section”. Should you have any questions regarding disposal. in particular any aggressive solutions.2. 9 Storage and transportation If the TitroLine alpha plus Titration Unit has to be stored over some time. Batteries should not be made part of the domestic waste. They will be taking back at no charge by the manufacturer and forwarded to proper reuse or disposal. and carry out the required reliability tests. also refer to chapter 8. . The bottom and rear side have to be cleaned dry. have to be drained. Cleaning − − The dosing units can be cleaned using a piece of cloth soaked in normal water or a household cleansing agent containing ethanol. or to be dislocated.

.....24 Computer connector Connector for temperature sensor probe….18 Control of the TitroLine alpha…………………22 Cylinder…………………………………………..23 Cleaning……………………………………….18 Connector for weighing-balance…………….... 18 EPROMs…………………………………………..18 Storage………………………………………….9 Measurement amplifier……………………….Reference List 161 11 Reference list Care…………………………………………….9 First operation………………………………….10 Keyboard connector……………………………19 Mains connector Mains switch Mains voltage Maintenance…………………………………….3 Printer port………………………………………18 Safety information……………………………….80 Warning information……………………… 9 ..3 Stirrer connector……………………………….17 Measurement sensor…………………………..9 Set-up……………………………………………10 Stirrer…………………………………………….24 Printer……………………………………………....9 Electrode connector………………………….3 Mini keyboard……………………………………3 Operating voltage Periods without operation…………………….

162 Notes: .

163 Notes: .

2. Certificat du fournisseur Nous certifions que le produit a été vérifié selon DIN EN ISO 9001. part 8.schottinstruments.Typ / Type / Type / Tipo: TitroLine alpha plus Bescheinigung des Herstellers Wir bestätigen.4 ”Sequimento y medición del producto” y que las especificaciones requeridas para el equipo son respetadas y cumplidas. Supplier’s Certificate We certify that the equipment was verified according DIN EN ISO 9001. parte 8. Printed in Germany SCHOTTInstruments GmbH Postfach 24 43 D-55014 Mainz Hattenbergstraße 10 D-55122 Mainz Tel. partie 8.4 „Überwachung und Messung des Produkts“ geprüft wurde und dass die festgelegten Qualitätsanforderungen an das Produkt erfüllt werden.2.4 « Surveillance et mesure du produit » et que les exigences spécifiées pour le produit sont respectées.4 ”Monitoring and measurement of product” and that the specified requirements for the product are met. Certificado del fabricanteNosotros certificamos que el equipo está verificada conforme a DIN EN ISO 9001.2.: +49 (0)6131/66-5111 Fax: +49 (0)6131/66-5001 E-Mail: titration@schottinstruments.2. dass das oben genannte Gerät gemäß DIN EN ISO 9001. Absatz 8.com EDV 008 27 007 4 Version 040714 ME .com www.