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Zi ehl –N eel sen st ai n - Wi ki pedi a, t he f r ee encycl opedi a

Ziehl–Neelsen stain
From Wikipedia, the free encyclopedia

The Ziehl–Neelsen stain, also known as the acid-fast stain, was first described by two German doctors: the bacteriologist Franz Ziehl (1859–1926) and the pathologist Friedrich Neelsen (1854–1898). It is a special bacteriological stain used to identify acid-fast organisms, mainly Mycobacteria. Mycobacterium tuberculosis is the most important of this group because it is responsible for tuberculosis (TB). Other important Mycobacterium species involved in human disease are Mycobacterium kansasii, Mycobacterium marinum, and members of the Mycobacterium avium complex. Acid fast organisms like Mycobacterium contain large amounts of lipid substances within their cell walls called mycolic acids. These acids resist staining by ordinary methods such as a Gram stain.[1] It can also be used to stain a few other bacteria, such as Nocardia. The reagents used are Ziehl–Neelsen carbolfuchsin, acid alcohol, and methylene blue. Acid-fast bacilli will be bright red after staining.

A variation on this staining method is used in mycology to differentially stain acid-fast incrustations in the cuticular hyphae of certain species of fungi in the genus Russula.[2][3] It is also useful in the identification of some protozoa, namely Cryptosporidium and Isospora. The Ziehl–Neelsen stain can also hinder diagnosis in the case of paragonimiasis because the eggs in an ovum and parasite sputum sample (OnP) can be dissolved by the stain, and is often used in this clinical setting because signs and symptoms of paragonimiasis closely resemble those of TB.

Mycobacterium tuberculosis visualization using the Ziehl–Neelsen stain.

Contents
1 Procedure 2 Modifications 3 See also 4 References 4.1 Online protocol examples 5 References 6 External links

Procedure
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. rinse with tap water Blot dry View under oil immersion lens Studies have shown that an AFB stain without a culture has a poor negative predictive value.Wi ki pedi a. this has a much higher negative predictive value. t he f r ee encycl opedi a 1. en.[4] See also Kinyoun stain Lowenstein-Jensen medium References "Microbiology with Diseases by Body System". 2006. 11. Paul A. Kinyoun modification (or cold Ziehl–Neelsen technique) is also available. 7. or g/ w ki i / Zi ehl –N eel sen_st ai n 2/ 3 . Wilson. Josephine A. Pearson Education. 8. Morello. and Verna Morton. 4. heat-fix slide 10 minutes at 90 °C Flood slide with Carbol Fuchsin Hold a flame beneath the slide until steam appears but do not allow it to boil Allow hot slide to sit for 3 to 5 minutes. Drop suspension onto slide Air dry slide 10 minutes at 60 °C. Modifications 5% sulfuric acid is used for destaining Mycobacterium leprae instead of the 20% used for Mycobacterium tuberculosis. 10th ed. Inc.12/ 26/ 12 Zi ehl –N eel sen st ai n . Granato. rinse with tap water Flood slide with 30% hydrochloric acid in isopropyl alcohol Allow to sit 1 minute. Laboratory Manual and Workbook in Microbiology: Applications to Patient Car. 9. 10. Bauman. Print. 2009. rinse with tap water Flood slide with Methylene Blue Allow to sit 1 minute. w ki i pedi a. 2. Marion E. 3. Robert W. Boston: McGraw-Hill Higher Education. 5. 6. A protocol in which a detergent is substituted for the highly toxic phenol in the fuchsin staining solution. An AFB Culture should be performed along with an AFB stain.

4. (1967).php?title=Ziehl–Neelsen_stain&oldid=522307566" Categories: Acid fast bacilli Microbiology techniques Staining Bacteriology Microscopy Navigation menu This page was last modified on 18 December 2012 at 14:13.nlm.ncbi. Wilson.utah.med. Mad River Press. en.PDF) protocol (PDF format). (1993). RC. ISBN 0-934454-87-6.edu/WebPath/HISTHTML/MANUALS/AFB.nih. ^ Largent.6.46. 3. D Johnson.org/10.gov/pubmed/7687254) . (1977). See Terms of Use for details. or g/ w ki i / Zi ehl –N eel sen_st ai n 3/ 3 . PMC 501296 (//www.. a non-profit organization.doi.gov/pmc/articles/PMC501296) . Boston: McGraw-Hill Higher Education. Wikipedia® is a registered trademark of the Wikimedia Foundation.pdf. w ki i pedi a.Wi ki pedi a. Text is available under the Creative Commons Attribution-ShareAlike License. doi:10. Paul A. and Verna Morton.559) .nih.gov/pmc/articles/PMC501296/pdf/jclinpath002070071. t he f r ee encycl opedi a Online protocol examples Ziehl–Neelsen (http://www-medlib.6. LA Zabrowarny. R Watling. "Safer staining method for acid fast bacilli" (http://www. ^ Morello. ^ Romagnesi.org/w/index. 10th ed. Les Russules d'Europe et d'Afrique du Nord.nlm. Journal of Clinical Pathology 46: 559–560. Granato.559 (http://dx.. How to identify fungi to genus III: microscopic features. 2. Josephine A.wikipedia.nih. PMID 7687254 (//www.ncbi.gov/pmc/articles/PMC501296/pdf/jclinpath00207-0071.ncbi.ncbi.nlm.1136/jcp. Marion E.nlm.12/ 26/ 12 Zi ehl –N eel sen st ai n . H. D. Laboratory Manual and Workbook in Microbiology: Applications to Patient Care. additional terms may apply.1136%2Fjcp.46. Print. Inc. External links Media related to Ziehl-Neelsen stain at Wikimedia Commons Retrieved from "http://en. Bordas. ISBN 0-916422-09-7. References 1. p 25.pdf) . http://www.nih. ^ Ellis. 2006.