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SBP PERFECT SCORE – BIOLOGY – SET 4 (P3

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SBP PERFECT SCORE BIOLOGY SET 4

PAPER 3

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An experiment to investigate the effects of temperature on the rate of an enzyme reaction is carried out by a group of student with the following steps:    Step 1: Step 2: Step 3: Fifteen drops of iodine solution were dropped separately in the grooves of a white tile. C and D. White tile Figure 2 shows the result obtained at the end of the experiment: Blue black solution Time taken for the complete hydrolysis of starch ___ minute Brown iodine solution Figure 2 Steps 1 to 4 are repeated by using the different sets of test tubes labelled as B. 1ml of saliva was put into a test-tube labelled as A. and the test tubes are immersed in water baths with temperatures at 25°C.SBP PERFECT SCORE – BIOLOGY – SET 4 (P3) Question 1 1. 35°C and 45°C respectively. The mixture was stirred and a drop of the mixture was taken out and dropped into the first groove of the tile containing iodine solution. Test-tube A was immersed into the water bath with a temperature of 15°C. 44 . Figure 1 shows the method used in the experiment: Thermometer Iodine solution Water bath ( 15˚C) 1 ml saliva Figure 1  Step 4: 5ml of starch suspension was added into test tube A. The iodine test was repeated every minute for 1 minute.

………………………………………………………………. [3 marks] 45 .SBP PERFECT SCORE – BIOLOGY – SET 4 (P3) Table 1 shows the observations of the drops on the white tile after each experiment: Temperature (°C) Time taken for the complete hydrolysis of starch (min) Test tube Observation on the white tile B 25 C 35 D 45 Table 1 1(a)(i) Record the time taken for the hydrolysis of starch to be completed at a temperature of 15°C in the space provided in Figure 2. (ii) Record the time taken for the hydrolysis of starch to be completed at different temperatures in the boxes provided in Table 1. ………………………………………………………………. ………………………………………………………………. 2 . [3 marks] (b) (i) State two observations on time from Figure 2 and Table 1 1..... ……………………………………………………………….

[3 marks] (c) Complete Table 2 based on the experiment that was carried out: Variables 1.SBP PERFECT SCORE – BIOLOGY – SET 4 (P3) (ii) State two inferences from the observations in (b)(i)... ……………………………………………………………….. Manipulated variable Particulars to be implemented How to alter the manipulated variable ……………………………………………. ………………………………………………………………. ……………………………………………………………………………. ………………………………………………………………. ………………………. Responding variable How to determine the responding variable ……………………………………………. ………………………… …………………………………………….. 2 . 3. ……………………………………………. 2. Controlled variables How to maintain the controlled variables …………………………………………….. ……………………………………………………………….. [3 marks] (d) State the hypothesis for this experiment …………………………………………………………………………….. [3 marks] 46 .. 1.. ………………………… …………………………………………….

[3 marks] 47 . Your table should contain the following titles:  Temperature  Time taken for reaction to be completed  Rate of reaction.. 1/time Temperature (ºC) Time taken (min) 15 25 35 45 Plot a graph of rate of enzyme reaction against temperature based on the table : Rate of reaction. ……………………………………………………………………………. 1/time (min-1) [3 marks] (e)(ii) Based on the graph in (e)(i). state the relationship between the rate of enzyme reaction with temperature of the medium ……………………………………………………………………………..SBP PERFECT SCORE – BIOLOGY – SET 4 (P3) (e)(i) Construct a table to record the results of this experiment.

……………………………………………………………………………... [3 marks] (h) Draw an arrow to match the enzyme with the substrate given in the boxes below: Amylase Peptidase Maltase Sucrase Pepsin Lipase Starch Sucrose Maltose Lipid Protein Peptide [3 marks] END OF QUESTION PAPER 48 . [3 marks] (g) If another set of experiment was prepared and immersed into the water bath at a temperature of 70°C. Give a reason for your prediction. predict the time taken for the enzymes to hydrolyse the starch completely. ……………………………………………………………………………... …………………………………………………………………………….SBP PERFECT SCORE – BIOLOGY – SET 4 (P3) (f) Based on the results of the experiment. what can you deduce about enzyme? …………………………………………………………………………….

light intensity and the presence or absence of nutrients. Your experimental planning need to include the following aspects:          Statement of identified problem Objective of study Variables Statement of hypothesis List of materials and apparatus Technique used Experimental procedures Presentation of data Conclusion the effect of pH. on 49 . design a laboratory experiment to investigate the activity of yeast. pH.SBP PERFECT SCORE – BIOLOGY – SET 4 (P3) Question 2 Microorganisms are not always active. The growth and activities of microorganisms are influenced by various abiotic factors such as temperature. Based on the above information.

SBP PERFECT SCORE – BIOLOGY – SET 4 (P3) Question 3 A group of students carried out an experiment to study the effect of light intensity on the rate of photosynthesis  Diagram 1(a)(i) shows the set up of the experiment.  Diagram 1(a)(ii) shows the time taken for the experiment  Diagram 1(b) shows the result of the experiment done at the distance of 20 cm.40 cm.60 cm and 80 cm from the bulb (menthol) Boiling Tube Gas bubbles beaker Aquatic plants Water bath (370C) Sodium hydrogen carbonate solution DIAGRAM 1(a)(i): SET UP OF THE EXPERIMENT Time taken for the experiment/min d Diagram 1 (a)(ii) 50 .

SBP PERFECT SCORE – BIOLOGY – SET 4 (P3) Diagram 1(b) : Result of the experiment For Examiner ’s Use 51 .

...............……………………………………………………………… …………………………………………………………………………… Observation 2: ……………………………………………………………………………… ……………………………………………………………………………… [3 marks] (ii) State the inference for each observation made in (a) (i).……………………………………………….. Inference for observation 1 ……………. Record the number of gas bubbles released in the space provided in diagram1(b) at the distance of 20 cm... Observation 1: ……………..SBP PERFECT SCORE – BIOLOGY – SET 4 (P3) 1(a) (i) Based on diagram 1(b).. state two observations that can be made from the experiment.................. 1 (a) (ii) 1(a)(i) …………………………………………………………………………………… [3 marks] (b)(i) (ii) Record the time taken for the experiment in the space provided in diagram (b)(i)(ii) 1(a)(ii).60 cm and 80 cm in the time given......………………………………………………. [3 marks] (c) Complete Table 1 based on the experiment that was carried out.. …………………………………………………………………………………… Inference for observation 2 ……………..... Variables Manipulated variable Particulars to be implemented How to alter the manipulated variable ……………………………………………… 52 .....40 cm......

SBP PERFECT SCORE – BIOLOGY – SET 4 (P3) ………………………. Distance (cm) 20 40 60 80 Number of gas bubbles released Rate of photosynthesis (Number of bubbles min -1) 1 (d)(ii) [3 marks] 53 .……………………………………………………………………… [3 marks] (ii) Record the result of the experiment in the table below.……………………………………………………………………………… 1(d)(i) ………………. ……………………………………………… Responding variable ………………………… How to determine the responding variable ……………………………………………… ……………………………………………… Controlled variable ………………………… How to maintain the controlled variable ……………………………………………… ……………………………………………… 1 (c) (d)(i) [3 marks] State the hypothesis for this experiment. ………. Plot a graph of rate of photosynthesis against distance.

SBP PERFECT SCORE – BIOLOGY – SET 4 (P3) (d)(iii) Explain the relationship between the rate of photosynthesis and the light intensity ………………………………………………………………………………………… … ………………………………………………………………………………………… … ………………………………………………………………………………………… … [3 marks] 1(d)(iii) (e) The experiment is repeated. but by increasing the temperature of the water bath to 600C.……………………………………………………… ……………………………………………………………………………………… ……………………………………………………………………………………… [3 marks] (f) State the relationship between the increase of temperature and the rate of photosynthesis. Predict the effect of increasing the water temperature to the rate of photosynthesis. 1 (f) 54 . 1 (e) ……………………………….

light bulb.……………………………………………………… ………………………………. sodium hydrogen carbonate powder.……………………………………………………… ……………………………………………………………………………………… ……………………………………………………………………………………… [3 marks] (g) Based on the experiment. By using a suitable plant that can absorb nutrient from nutrient solution (aquaculture). phosphorus and potassium are most important. Nitrogen as in nitrate. phosphorus and potassium deficiency on this plant. ………………………………. [3 marks] 1 (g) 1 (h) Question 4 Plant needs macronutrients and micronutrients to grow healthy and reproduce.……………………………………………………… ……………………………………………………………………………………… [3 marks] (h) Another group of students had carried out the experiment to study the effect of carbon dioxide concentration on the rate of photosynthesis but using the following apparatus and materials. Categorised the apparatus and materials in their experiment. distilled water. design an experiment to show the effect of nitrogen. define photosynthesis operationally.SBP PERFECT SCORE – BIOLOGY – SET 4 (P3) ………………………………. Hydrilla sp. Your experimental planning need to include the following aspects:          Statement of identified problem Objective of study Variables Statement of hypothesis List of materials and apparatus Technique used Experimental procedures Presentation of data Conclusion 55 .