13 views

Uploaded by Tarun Meena

DDP

- Lesson12_Material_Property_Definition.pdf
- Tube Sheet Paper
- Constitutive Modeling of layered concrete shells.pdf
- Df 31729732
- Finete Element
- Notes - FEM
- J.N.Reddy
- Naca Arr3k04[1]
- APEGBC-Eng-Syllabus-Structural.doc
- Fem
- Finite Element Modelling of Flexible Pavement.pdf
- Live Cell Imaging of Actin
- FEM - Nonlinear
- Why_we_need_MSCR_8_25_2011
- FEM analysis of pavement linear and non linear.pdf
- Optimizations of Outer Door Handle in Automobiles using Composite Material
- 631_638
- 4
- Site Response
- 2-intro_truss_1lt+2th

You are on page 1of 38

Introduction

E.g. cells must able to maintain their shapes when

The varied mechanical behaviour of cell is determined

of filament network

We discuss the rheology of F-actin networks and

highlight how the small change in network composition can qualitatively change the mechanical response

Cytoskeleton

Establishing cell shape Providing mechanical strength Locomotion and muscle fiber contraction Cell motility Chromosome separation Cell motility Ensure proper division of cell during cellular production

Existing Models

Elasticity of Stiff Polymer Networks This model is about the elasticity of a two dimensional network of rigid rod

The random network was generated by placing N line

is defined as below

Continued..

Figure: Typical networks at high and low densities. The distribution shown in false colors; the load on filament increases from blue to red. the left network has 99.99% of the strain energy stored in bending modes. In contrast, the right network has only 5% of the energy stored in bending; the remainder is stored in compression mode.

Continued..

rods, where compressional stiffness is lower or comparable to the bending stiffness, the shear modulus scales linearly with the filament compressional modulus and the number of filament per unit area.

Continued..

Elasticity of Planar Network In this model the fiber axial deformation was considered This model account for microscopic deformation for the elastic stiffness of planar Filament Networks with all possible lengths and orientations. They suggested that the effective stiffness of a planar random Filament Network increases with the increase of Arial density Arial density, which can be measured by the number of fibers per unit area(fiber concentration)

Continued..

Continued..

The constitutive relation derived in this case for the effective stiffness of random Filament Network is as follows

Where,

Continued..

Passive and Active micro-rheology for crosslinked F-actin networks in vitro According to the model if the length of the filament is comparable to mesh size many loose end exist which contribute little to stiffness of the network

Reducing the filament length leas to more loose ends,

thereby altering network connectivity, as a result network is less capable of withstanding stress and therefore exhibit smaller modulus

Continued..

(A) (B) Figure : F-actin network organized by long(A) and short(B) actin filaments at identical concentration of actin filaments and cross linkers

Continued..

filaments(A) is regularly arranged while the network with short filament length(B) forms incomplete loops with many loose ends would cause the network with short filaments to be less stiff than the one with long filaments

technique for approximate solutions to a variety of real world engineering problems The basic idea of the finite element method is to break up a continuum into discrete number of small elements and expressing the unknown field variables in terms of the assumed approximating functions within each element FEM package such as ABAQUS enable to solve more complex element behaviour to be model

While modeling several assumptions have to made in order to simplify and practically model the actual system. The Key assumptions are as follows The fibers are straight and oriented in the same plane The fibers are randomly distributed The deformation is linearly elastic Beam cross section is assumed to be circular Effect of the medium, in which fiber networks is, being assumed negligible

For the filament network analysis, step by step procedure is as follows Sketched the two dimensional geometry of the filament network and created a part representing the network Defined the material properties and section properties of the network Assembled the model and created instance of the geometry Meshed the frame

Continued..

filament network Created job named fil Network and submitted for analysis Observed the analysis result The above procedure was repeated each time for change in network composition separately

of independent short line segments on a two dimensional plane. In the network, at y=0;x=0 and x=L hinge and roller condition is applied Varying Displacement is provided at y=L i.e. at the top of the surface For the fixed value of displacement force has been calculated

Continued

Figure : Model of the Network with vertical displacement and support condition

Continued..

procedure The reaction is obtained on the upper surface of the network and summed to the total reaction force in ydirection Elastic modulus of the network can be calculated as

RF2 Ec L disp. L

Effective elasticity of the filament network The load-displacement curve is obtained for a network with Length of fiber 20m

Elasticity of the fibers is assumed to be

E=2x1011 N m2

for

the analysis

Continued..

(A) (B) Figure Model of the Network with vertical displacement and support condition (A) and its deformed shape (B)

Continued..

Fig: Reaction Force Vs Displacement of the filament Network

4,50E+10

4,00E+10 3,50E+10 Reaction Force(N)

3,00E+10

2,50E+10 2,00E+10

1,50E+10

1,00E+10 5,00E+09

0,00E+00

0 0,2 0,4 0,6 0,8 Displacement(m) 1 1,2 1,4 1,6

Continued..

The effective elasticity of the network. i.e. ratio of the elasticity of the network with the elasticity of the filament,

Ec =0.14 E

To study the effect of variation in elasticity two models

Applying BCs hinges and rollers at x=0,y=0 and x=50m

Keep the length of the fibers 20m for both of the

Continued..

(A) (B) Figure : Low density Network(A) and High density Network(B)

Continued..

(A) (B) Figure : Deformed shapes of the low Density network (A) and High Density Network (B)

Continued..

Reaction Force Vs Displacement

4,50E+10 4,00E+10 3,50E+10 Reaction Force(N) 3,00E+10 2,50E+10 2,00E+10 1,50E+10 Fig: Reaction Force Vs Displacement of low density Network Fig: Reaction Force Vs Displacement of High density Network

1,00E+10

5,00E+09 0,00E+00 0 0,5 Displacement(m) 1 1,5

Figure : Plot of Reaction Force Vs Displacement for low Density network (A) and High Density Network (B)

Continued..

2.822E+10 N m2

density network)comes out to be 3.00E+09 N2 while m elasticity of the network B(high density network) is

increase in density of the network The above result can also be verified with the similar model developed by X.-F. Wu, Y. A(6) where they suggested that the effective stiffness of a planar random Filament network increases with the increase of elasticity

short filaments and network with long filaments

x=100m

Analysis and comparison of both of the network is as

follows

Continued..

(A)

(B)

Figure : F-actin Network organized by long (A) and short (B) actin filaments at identical concentration of filaments and intersections

Continued..

(A) (B) Figure : Deformed shape of F-actin Network organized by long (A) and short (B) actin filaments at identical concentration of filaments and intersections

Continued..

Reaction force Vs Displacement

5,00E+10 4,50E+10

4,00E+10

3,50E+10 Reaction force(N) 3,00E+10 2,50E+10 2,00E+10 1,50E+10 1,00E+10 5,00E+09 0,00E+00 Fig: Reaction Force Vs Displacement of Network with short filaments(B) Fig: Reaction Force Vs Displacement of Network with long filaments(A)

0,5

Displacement(m)

1,5

Figure : Plot of Reaction Force Vs Displacement for of F-actin Network organized by long (A) and short (B) actin filaments

Continued..

1.14E+10 N m2

2

with long filament comes out to be 3.38E+10 N while m the elasticity of the network B with long filament is

more compare to the network B with short filament The above model can be verified with similar type of model which is given by Lee, Ferrer ,Nakamura (8)

Continued..

individual filament lead to more loose ends in the configuration, thereby altering the network connectivity The resulting effect is that the network is less capable of withstanding stresses and therefore exhibit smaller modulus

Bibliography

1. (n.d.). Retrieved from Wikipedia: http://en.wikipedia.org/wiki/Cytoskeleton 2. Risler, T. (2011). Cytoskeleton and Cell Motility. 3. Abed, G. (2010). COMPUTATIONAL MECHANICS TOWARDS IMPROVED UNDERSTANDING OF THE BIOMECHANICS OF MYOCARDIAL INFARCTION. 4. David A. Head, A. J. (2003). Deformation of Cross-Linked Semiflexible Polymer Networks. PHYSICAL REVIEW LETTERS , 91 (10), 108102. 5. Frey, J. W. (2008). Elasticity of Stiff Polymer Networks. 6. X.-F. Wu, Y. A. (2005). Elasticity of planar fiber networks. JOURNAL OF APPLIED PHYSICS , 98, 093501. 7. R.Y. Kwon, A. L. (2008). A microstructurally informed model for the mechanical response of three-dimensional actin networks. Computer Methods in Biomechanics and Biomedical Engineering , 11 (4), 407418. 8. Hyungsuk Lee,Jorge M. Ferrer,Nakamura(2010). Acta Biometerialia,6,1207-1218 9. F. C. MacKintosh, J. K. (1995). Elasticity of Semiflexible Biopolymer Networks. P. A. Janmey , 75 (24).

Continued..

10.G. A. Buxton, N. C. (2009). Actin dynamics and the elasticity of cytoskeletal networks. eXPRESS Polymer Letters Vol.3, No.9 , 579-587. 11. Gardel, M. L. (2004). Elasticity of F-actin Networks. 12. Jeffrey S. Palmer, M. C. (2008). Constitutive modeling of the stress strain behavior of F-actin filament networks. Actabiomaterialia , 4, 597612. 13. Jonathan Stricker, T. F. (2010). Mechanics of the F-actin cytoskeleton. Journal of Biomechanics , 43, 914. 14. Madenci, E. (2005). The Finite Element Method and Applications in Engineering Using ANSYS (1st ed.). Springer. 15. Margaret L. Gardel, *. K. (2008). Mechanical Response of Cytoskeletal Networks. In M. L. Gardel, METHODS IN CELL BIOLOGY (Vol. 89, pp. 487-519). 16. ABAQUS CAE documentation. (n.d.). Retrieved from http://abaqus.civil.uwa.edu.au:2080/v6.9/

Thank You

- Lesson12_Material_Property_Definition.pdfUploaded bySaravanan Srvn T
- Tube Sheet PaperUploaded byJavier Ordieres
- Constitutive Modeling of layered concrete shells.pdfUploaded bysahashi
- Df 31729732Uploaded byAnonymous 7VPPkWS8O
- Finete ElementUploaded byBimalKrishna
- Notes - FEMUploaded byKamal Nikhurpa
- J.N.ReddyUploaded bypvmoorthi
- Naca Arr3k04[1]Uploaded byw824rg2
- APEGBC-Eng-Syllabus-Structural.docUploaded byRay Gong
- FemUploaded byajith kumar
- Finite Element Modelling of Flexible Pavement.pdfUploaded byHenry Abraham
- Live Cell Imaging of ActinUploaded byIvani Yunita
- FEM - NonlinearUploaded bysteven_gog
- Why_we_need_MSCR_8_25_2011Uploaded byEdgardo A. Chavarria
- FEM analysis of pavement linear and non linear.pdfUploaded byHanamant Hunashikatti
- Optimizations of Outer Door Handle in Automobiles using Composite MaterialUploaded byIRJET Journal
- 631_638Uploaded byCarlo Galli
- 4Uploaded bykrishnanunni
- Site ResponseUploaded byesscindia
- 2-intro_truss_1lt+2thUploaded byNovemberRain
- JournalNX- Dental Material AnalysisUploaded byJournalNX - a Multidisciplinary Peer Reviewed Journal
- Un Modelo Geométrico de Matriz de Inclusión Para El Análisis de Elementos Finitos Del Concreto a Múltiples EscalasUploaded byAaron Arango Ayala
- 1Uploaded byvaliente_11
- ch07_ex.pdfUploaded byTom
- Mech Props Slides- Remix1 (3)Uploaded byLeighton Thompson
- Midterm RevisionUploaded bySaleh
- settlementUploaded byBJER
- Iss32 Art1 - Numerical Simulations of Geotechnical Works in Bangkok SubsoilUploaded byJuan Manuel Velazquez Gonzalez
- Mechanics of MaterialsUploaded byaap1
- 68 Palmstrom&Singh on Deformation ModulusUploaded byClovis Gonzatti

- Dynamics of filament GrowthUploaded byTarun Meena
- HPCL call letter.pdfUploaded byTarun Meena
- DDP 2 ReportUploaded byTarun Meena
- Allocation_List_ESE_2011.pdfUploaded byTarun Meena
- Indian Institute of Technology Delhi[1]Uploaded byTarun Meena
- DDP STAGE-I PresentationUploaded byTarun Meena
- Ex Beam ColumnsUploaded byTarun Meena
- Civil Eng MainsUploaded byTarun Meena
- PDF-BSNL JTO 2009 Civil EngineeringUploaded byTarun Meena
- Lecture on Plates and ShellsUploaded bykhers_zal
- DDP STAGE-I PresentationUploaded byTarun Meena

- Three Moment Equation PaperUploaded byAkash Sood
- Uniaxial Tension Test - The Mother of all Mechanical Tests By Kartik Srinivas, Owner AdvanSESUploaded byRaasik Jain
- CHE572 Chapter 5 Hopper Design.pdfUploaded byMuhd Fahmi
- 06 - Resist Flow Thru Piping ElementsUploaded byManuel Antunes Luis
- Shim_Alvin_1770934_Tues_430Uploaded byshimmy
- Topic 01-Basic Principles in Fluid MechanicsUploaded byLeo Lai
- Core Practical 4Uploaded byDanKimberley
- Sissala - FEA Simple Tension Test Progressive FailureUploaded byYoun Seok Choi
- Comparative Analysis of Symmetrical and Asymmetrical L-Shape & T-Shape Multistoried Building under Seismic Load and Gravity Load With Varying Stiffness.Uploaded byIJSTE
- formula studentUploaded byChirila Razvan
- Introduction to ANSYS CFX Workshop 04 AxUploaded byLam Trinh Nguyen
- curved surface hydrostaticsUploaded byDheeraj Thakur
- SSC JE 2013 100-200 mechUploaded byjiteshpaul
- Csanady Air Sea InteractionUploaded byEduardo Salgado Enríquez
- Chapter 2 Conduction AnalysisUploaded byNurul Asyilah
- 01_11Elasticity (183-197)Uploaded byeamcetmaterials
- Jovan Bebic Precise Input-output Energy MeasurementUploaded bycorrynt
- Vehicle AerodynamicsUploaded byHelloWorld
- Yield StrengthUploaded byShanmuga Sundaram
- II Sem 2 Marks QuUploaded byGnana Ambica
- Teaching physics during the 17th century: some examples from the works of Evangelista TorricelliUploaded byAmelia Carolina Sparavigna
- Manhole AnalysisUploaded byhasan_kygsz
- Physics Mc Forces Newtons LawsUploaded byT W
- Bonded versus unbonded strip fiber reinforced elastomeric isolators: FEAUploaded byDaniele Di Luca
- Mechanical Reasoning ReviewUploaded byking gh
- Gas Dynamics-Nozzles and DiffusersUploaded byRahul
- Handbook - ABB Flowmeters (9!9!05)Uploaded byRubén Jimenez Castro
- Machine Design Reviewer.pdfUploaded byRobert
- Storage Tank DesignUploaded byravi456comp
- Stabilitas KapalUploaded bytuteng