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Personal Protective Equipment (PPE) All employees handling hazardous materials must wear the appropriate PPE when

necessary. Standard lab PPE includes a lab coat, safety glasses, and gloves such as powder-free SafeSkin, N-dex, or NeoPro, and closed shoes. The two most common routes of exposure when handling chemicals in the lab are inhalation and skin contact or absorption. Handling chemicals in the fume hood reduces the inhalation hazard and provides protection from splashes. Use of proper gloves and other PPE will prevent skin exposure and damage.
6.1 Gloves

Gloves are used to protect hands and in some cases portions of the arms from coming into contact with a hazard. In the laboratory the main hazards involve chemicals, biological material, radioactive materials, sharp objects, and extreme temperatures (autoclave, liquid nitrogen).
6.1.1 Selection

Glove selection is based on the following factors to determine the best option for each task. 1. Chemical and physical hazard(s) For chemical hazards the best source of information is a chemical resistance chart or database. These are available from many manufacturers and provide guidance on a glove’s compatibility with a chemical. This information will guide selection of a glove’s material and thickness. The information available may not include every glove nor compatibility data for every chemical. Manufacturers provide varying degrees of information and may have different definitions of permeability, permeation rate, and degradation rating. They also use different rating systems making comparison between manufacturers difficult at times. Links to several manufacturer’s charts or databases are located in section 6.1.8. Material safety data sheets may also provide some guidance on the selection of glove material. Glove selection must also take into account non-hazardous materials. For example, Polyvinyl Alcohol (PVA) gloves work very well for protection against some organic solvents but the coating is water soluble and will quickly degrade when in contact with an aqueous solution.

polyvinyl chloride.1. Contact EH&S if you have any concerns or questions or would like guidance with glove selection. If chemical contamination occurs while wearing disposable gloves. They are not designed for applications involving prolonged.1. remove the gloves and dispose of them in the regular garbage. Table 6. If you need assistance choosing the proper glove. nitrile rubber. Potential contact time and splash hazard The higher the potential contact time or risk of splash (by nature of the task or volume) requires higher protection via a thicker or longer glove. Disposable gloves must not be reused. Nitrile gloves provide protection from a wider range of hazardous materials and are more resistant to tearing. A thicker glove may provide better protection but loss of dexterity may increase the risk of a spill. If contamination results from incidental contact (small amounts of chemicals that will dry quickly). Pouring a substance would likely require more protection than pipetting work due to the risk of splashing during pouring. but instead for incidental splash exposures. 2. Leather and metal mesh gloves offer good resistance to bites. 3. Disposable latex gloves typically offer sufficient protection when handling small quantities or diluted chemicals with a low chance for contact or splash. hands or forearms into a hazardous material would require greater protection than from incidental contact with small volumes.2 Disposable Gloves (small quantity handling) Disposable gloves are thin (< 8 mil) and most commonly made from latex rubber.2. glass punctures and cuts.Common physical hazards in the laboratory involve sharp objects or extreme temperatures.6. Gloves appropriate for liquid nitrogen are discussed in Section 12. Looped terry cloth gloves are a good option for handling autoclaved items assuming they are dry and cool. immediately remove and discard the gloves. or Neoprene.1 shows glove recommendation for handling small quantities of common lab chemicals. direct exposure to chemicals. 6. Dexterity A balance must be struck when selecting gloves when hazardous materials are involved with precise tasks. contact EH&S. Immersing fingers. If gloves are grossly .

Contact EH&S for assistance in determining the best disposal option for gloves contaminated by a particular chemical.1: Glove Recommendations for Small Quantity Handling Hazardous Material Acetic acid Acetone Acetonitrile Acrylamide Chloroform DMSO Ethanol Ethidium bromide Methanol Phenol Sodium hypochlorite Blood and body fluids Radioactive isotopes Latex Exam Gloves* NT NT NT NT NT NT NT NT NT NT NT x x Best N-Dex 7005 (4 mil) x x x x x x x x x x x NT = Not Tested x = Best Glove Choice *Powdered latex gloves are prohibited. swelling. they should be collected as hazardous waste in a plastic bag as described in Chapter VI.3 Reusable Gloves (large quantity handling) Reusable gloves are thicker and offered in a wider range of materials. saturated with. Table 6. etc.1.1. Once contaminated: they may be washed and dried.2 shows glove recommendation for handling large quantities of common lab chemicals. The most common are latex rubber. These gloves are necessary when handling large quantities or chemicals where significant contact or splash is likely. nitrile rubber. Neoprene. wash your hands and don a new pair of gloves.1. After removing contaminated gloves.2.1.. and if found. Table 6. Solid Chemical Wastes.contaminated (were immersed in. holes. cracking.4.1. Viton.1: Specialty Gloves for Large Quantity Handling Hazardous Ansell Ansell Best NitroNorth Material Edmont Edmont Solv 727 Butyl Natural Neoprene Rubber Rubber (15 mil) Ansell Ansell Edmont Edmont Supported 4H PVA . or butyl rubber. Signs of degradation include tears. 6. the gloves must be properly disposed as outlined in 6.2.1. Table 6.2. or are still wet with chemicals). hardening. Section 4.2.

or non-lab floors. cafeterias (or other eating areas). library. restrooms. If you are wearing gloves.1. it will prevent contamination of your gloves if they are being used to protect the material you are handling. conference rooms. Factors that exacerbate the severity . the duration of exposure.Acetic acid Acetone Acetonitrile Acrylamide Chloroform DMSO Ethanol Ethidium bromide Methanol Phenol Sodium hypochlorite √ √ √ (15 mil) √ √ √ √ √ √ √ √ √ √ (17 mil) √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ = Best Glove Choice 6. Conversely. it has been determined that the corn starch powder is the carrier of the latex allergen. its concentration.4 Gloves Use Restrictions Gloves should not be worn in elevators.1.5 Latex Allergies A latex allergy is an allergy to products made from natural rubber latex. It is usually a reaction to proteins in the rubber that are still present in products made from natural rubber latex. This will prevent contamination of facilities and other personnel if your gloves are contaminated. Use a secondary container to transport materials. 6. Latex allergies can produce a variety of symptoms. A recent study demonstrated that corn starch binds with the allergenic latex proteins and transports them by direct contact with the skin and/or by exposure to airborne particles. In the case of powdered latex gloves. The severity of the reaction depends on the potency of the allergen. and the individual’s sensitivity. remove them before answering the phone or touching equipment or doorknobs. stairs. It will allow you to remove your gloves as well as protect your work. offices.

dentists. In general. compared with 1% in the general population.8 Glove Charts and Databases . and the Occupational Health Nurse (OHN) of allergies before receiving care.1. wearing latex over nitrile gloves will provide adequate protection without impeding dexterity. but does permit the use of nonpowdered latex gloves. This is to prevent employees from developing latex allergies and to protect employees who have latex allergies. a nitrile glove will provide better protection when handling chemicals and is recommended. Nitron One. pressure. If you prefer a powdered glove. but still need good dexterity to perform the work.6 Powdered Latex Prohibited The Center prohibits the use of powdered latex gloves. There is no treatment available for latex allergies. Wear an allergy bracelet. It is important to double glove when handling millicurie amounts of 125I or 131I. The only means of controlling allergic reactions is strict avoidance of latex exposure. 3. The powder carries the latex and. the rate of latex allergy prevalence among lab and healthcare workers has been estimated between 6-14%. If you are handling a highly toxic or a dangerously toxic chemical. Double gloving is required when handling HIV cultures or when human blood routinely contacts gloved hands. you can use a powdered nitrile glove such as Microflex. Employees who have been diagnosed with latex allergies should: 1. Carry and use non-latex gloves and/or non-powdered latex gloves.1.7 Doubling Gloving Double gloving is recommended under several circumstances to provide adequate protection.1. 2. Currently. it can trigger a response in those who are sensitive. and previous dermatological disease. when airborne. 6. changing the outer glove frequently. sweating. 6. The airborne powder contributes to the development of allergies to latex.of the contact dermatitis include friction. Inform physicians. 6. or N-dex.

pdf N/A Best Kimb erlyClark Micro flex N/A http://www. · Permeation Rate: Highest rate at which the chemical migrates after breakthrough has occurred.aspx (for Microflex thin latex and nitrile gloves). re. Some manufacturers rely on reference materials while others use data from one glove for all gloves of similar material and thickness.kcprofessional. Note: it is very important to understand each manufacturer’s definitions and source of data. softening.ansellpro. swelling. manufacturers use the following for assessing glove chemical resistance.microflex.Glove recommendations in the charts and databases are typically based on permeation and degradation testing. · Breakthrough Time: Time it takes for the chemical to travel through the glove material. Others only publish data for testing done on each glove material and thickness and communicate if a glove has not been tested against materials in the table.2 Lab Coats . · Degradation Rating: Evaluates any changes in the physical properties of the material which includes hardening.asp (for thin lab gloves.ansellpro. select Microflex Chemical Resistance Guide) 6. cracking. select Splash Guide results) hemrest/ (select US then Chemrest – Chemical Resistance Guide) http://www. Manuf Interactive Database acture Ansell _8thEditionChemicalResistanceGuide. Below is a list of some common vendors with links to their glove interactive database or charts. In are/ s/mkt/ChemicalSelectorGuide/ N/A Chart For thicker gloves only: http://www.

1 Lab Coat Pick-up and Drop-off Locations New lab coats are picked up and soiled coats returned to the following locations: Building Fairview Hutchinson PHS Thomas Weintraub Distribution Location LF-260-3. Measure the widest portion of your chest and choose a lab coat 1 to 2 sizes larger. Thomas.northwest corner. 2. 3.2. body fluid. PI or supervisor name. A vendor provides weekly laundry service for all the lab coats.Lab coats are available for all employees whose jobs involve direct use of hazardous materials (radioactive. . for wearing ease. Generic lab coats with Hutchinson Center logos are available for laboratory employees who handle hazardous materials in the Weintraub. Fill out the Lab Coat Order Form or call in an order directly to EH&S. Name. and infectious materials). Employer (the Hutchinson Center).fhcrc. chemical. The new generic coats will be available in a range of sizes in each location.2. The lab coats range from 34 to 54 (even sizes). Personalized lab coats in these locations are not provided by EH&S. Mailstop. past the vending machines E-Level Clinic: ME-B502 Fifth floor labs: Hallway next to M5-A402 Alcove: Cross-hallway of E-level. 4. and PHS buildings. west corner C1M-029: First level interstitial. Building C. 7. Worksite. near the west set of elevators AD-115: Near the D-level elevators 6. Hutchinson. blood. 6. Hazardous materials you work with. 2nd floor.2 Ordering Lab Coats Only PHS clinic lab coat users may order new lab coats from EH&S. Lab coat size. Please take only one coat as needed. The lab coat service was approved by the Health & Safety Committee and the Space and Resources Committee and there are several locations throughout the Center where lab coats are 6. Please include the following with your request: 1. and return it to the hamper when it becomes soiled. and 8. Extension. See the EH&S website for more information: centernet. 5.

1 Safety Glasses Approved safety glasses are those which meet the American National Standards Institute (ANSI) Z87 testing criteria. .Please allow up to three weeks for delivery of your new lab coat.3 Contacts If you choose to wear contacts when handling chemicals. fit test. Do not remove side shields from these glasses. label. EH&S will medically clear. 6. 6. seal. place the coat in a plastic bag. seal. or Risk Group 1 (BL-1) agents: Place the coat in the provided red hamper for laundering. and hold for EH&S pickup. Safety glasses offer good impact protection. then place the coat in the provided red hamper for laundering. seal. Chemical: If the chemical is sewerable and the contamination is minor.3. Radioactivity: Place the lab coat in a plastic bag. 6.2 Goggles The eyes and face should be protected from potential splashes by conducting work with hazardous substances in a chemical fume hood.2. The vendor treats all coats as though they are contaminated with any of these agents. In the event of a chemical splash to the eyes. rinse with water. Chemical splash goggles underneath a face shield must be worn when a chemical fume hood is not used and there is a potential for a hazardous substance to splash.3 Eye protection As a minimum standard. 3.5 Foot protection . Blood.3. If it is gross contamination or contamination with a select carcinogen (as defined in Chapter III). 6. and hold for EH&S pick-up. and issue a cartridge respirator to an employee if there is a potential for overexposure.3. 6. 6. Do not purchase respiratory protective devices of any kind without EH&S approval. be sure to wear safety glasses. safety glasses must be worn whenever handling hazardous materials.3 What to Do if Your Lab Coat Becomes Contaminated With . body fluids. contacts can hold the chemical against the eye prolonging the exposure and increasing eye damage. 1.4 Respiratory Protection Lab ventilation and chemical fume hoods typically control exposure to hazardous chemicals. Contact EH&S if a loaner coat is necessary. label. 4. 6. but very limited splash protection. If you believe a hazardous airborne exposure condition may exist in your work area. train. BL-2 agents: Place the lab coat in an autoclave bag. Approved glasses are marked with the number Z87 on the inside of the temple. label. . and hold for EH&S pickup. contact EH&S. 2.

Employees working in a lab must wear sturdy-soled. well-fitting shoes that cover the entire foot .. Crocstm). . and open-toe shoes are not acceptable in labs.g. perforated shoes (e. Leather shoes are recommended. Sandals. slip-ons.