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Riyani Wikaningrum Bag. Mikrobiologi FK Univ. YARSI

Sampling Technique .

Three-glass Procedure .

Meares and Stamey localization technique .

Expose the penis and retract the foreskin so that the glans is exposed. should be removed. Hands are washed 4. Cleanse the glans with a soap solution. the patient should drink 400 ml of liquid (two glasses). Urinate 10–15 ml into the first container marked VB1 .1. which are marked VB1. Place the uncovered specimen containers on a flat surface and maintain sterility 3. VB2. The test starts when the patient wants to void 2. Approximately 30 minutes before taking the specimen. The lids of four sterile specimen containers. EPS and VB3. The foreskin should be retracted throughout 5. remove the soap with sterile gauze or cotton and dry the glans 6.

The patient bends forward and holds the sterile specimen container (EPS) to catch the prostate secretion 9. urinate 10–15 ml into the second container marked VB2 8. The physician massages the prostate until several drops of prostate secretion (EPS) are obtained 10. the patient urinates 10–15 ml of urine into the container marked VB3. If no EPS can be collected during massage. Urinate 100–200 ml into the toilet bowl or vessel and without interrupting the urine stream. .7. Immediately after prostatic massage. a drop may be present at the orifice of the urethra and this drop should be taken with a 10 ml calibrated loop and cultured 11.

Proteus sp.Prostatitis: • Akut . aeruginosa Serratia sp. Klebsiella sp. aureus. Enterococcus.Disebabkan oleh: – – – – – – – E. coli P. faecalis. S. Enterococci Staphylococcus • Kronik – dibagi menjadi 3 sindrom: • Chronic bacterial prostatitis • Chronic abacterial prostatitis/CPPS-inflammatory • Chronic abacterial prostatitis/CPPS-non-inflammatory – Disebabkan S. difteroid .


Prostatitis • Pemeriksaan laboratorium: – MSU – EPS (expressed prostatic secretion – digital rectal massage): • Lepas antibiotik selama 1 bulan • Tidak mengalami ejakulasi selama 2 hari .

coli Koliform lainnya .Epididymo-orchitis • Penyebab: – – – – – – Mumps virus Coxsackievirus N. trachomatis E. gonorrhoeae C.

Terminology Used in UT infections .

• “Upper UTI” also encompasses – intrarenal abscess (“renal carbuncle”) – perinephric abscess. •  pyelonephritis. the ureters.Upper Urinary Tract Infection • the urinary tract above the level of the bladder. kidneys. . • Renal papillary necrosis refers to infarction of the papillae (sometimes with sloughing into the ureters)  caused by pyelonephritis or analgesic abuse – diabetes mellitus – sickle cell disease – ureteral obstruction. and peri-renal tissues. that is.

Acute bacterial pyelonephritis • clinical syndrome – Fever – flank pain – often with constitutional symptoms • Laboratory findings: – Leukocytosis – leukocyte casts in the urine – bacteriuria with or without signs of concomitant inflammation in the bladder .

and infection of the periurethral glands.Lower Urinary Tract Infection • Lower urinary tract infection refers to infection at or below the level of the bladder. and variable suprapubic tenderness. frequency. Because one cannot say with certainty that infection involves mainly or exclusively the urinary bladder.” a syndrome characterized by dysuria. urgency. “Lower UTI” also encompasses prostatitis. “lower UTI” is often used synonymously with “cystitis. In clinical practice. . urethritis. some authorities suggest that “cystitis” should be abandoned.

– Etiology : Drugs (not only prescription drugs but also non-prescription drugs as in analgesic nephropathy) .Chronic bacterial pyelonephritis • long-standing infection with active bacterial growth in the kidney. or the presence of residual lesions in the kidney caused by such infection in the past. • Chronic interstitial nephritis  – in which evidence for an etiologic role for bacterial infection is lacking.