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Code No: R05222303 Set No.

1
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. What does “right handed helix” & “left handed helix” mean? Explain taking
suitable examples. [16]

2. How does rolling circle replication produce multimers of a replicon? [16]

3. How is the lagging strand synthesis different from the leading strand synthesis in
DNA replication? [16]

4. Justify the statement “there are different types of rRNA found in ribosome” [16]

5. Using suitable examples explain how they are acting as inhibitors of transcription.
[16]

6. What is meant by “Wobble hypothesis”? Explain its connection to the genetic


code. [16]

7. How is the initiation of translation process occurring in prokaryotes and eukaryotes?


Elaborate briefly. [16]

8. Write short notes on [2 × 8 = 16]

(a) Thiamine dimmers and photo reactivation


(b) Excision type of DNA repair

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Code No: R05222303 Set No. 2
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. What does “right handed helix” & “left handed helix” mean? Explain taking
suitable examples. [16]

2. Using neat diagrams explain a circular chromosome undergoing bi-directional semi


conservative replication. [16]

3. Explain the process of DNA replication in detail. [16]

4. Discuss in detail the structure of the organelles functioning as sites of protein


synthesis. [16]

5. Using suitable examples explain how they are acting as inhibitors of transcription.
[16]

6. In the genetic code, how can one justify the presence of 64 codons coding for only
20 amino acids. [16]

7. Once a protein is synthesized, can it be modified? Justify your answer giving


suitable examples. [16]

8. With the help of a neat diagram explain how the process of site directed mutagenesis
is done. [16]

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Code No: R05222303 Set No. 3
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Write a note on the different forms of the double helix DNA and elaborate on “B”
form. [16]

2. How does the bacteriophage Φ×174 replicate through rolling circle mechanism?
[16]

3. What is meant by ‘end replication problem’ in DNA synthesis? Discuss the role of
telomase in it. [16]

4. Write about the different types of ribonucleic acids. [16]

5. Where does the synthesis & processing of pre-rRNA occur? Explain the process.
[16]

6. Write briefly about the steps of initiation, elongation and termination in the process
of prokaryotic translation. [16]

7. What are post transnational modifications? Give some examples. [16]

8. How is reverse genetics approach used to study a gene function? [16]

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Code No: R05222303 Set No. 4
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Explain how GC base pair content of a ds DNA affects the melting temperature
Tm. [16]

2. With respect to DNA replication, explain

(a) Origin of replication


(b) Replication fork
(c) Bidirectional replication
(d) Semi-conservative replication. [4 × 4 = 16]

3. How does DNA replication proceed in the 5’ − > 3’ polarity parental DNA strand?
[16]

4. Give a step-by-step description of the process of Transcription. [16]

5. Describe briefly about the posttranscriptional processing occurring in eukaryotic


mRNA. [16]

6. In the genetic code, how can one justify the presence of 64 codons coding for only
20 amino acids. [16]

7. With context to gene transfer mechanisms, explain [2 × 8 = 16]

(a) Transduction
(b) Conjugation

8. Explain how base analogs and alkylating agents act as chemical mutagens in in-
duced mutations. [16]

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