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Code No: RR222305 Set No.

1
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. In what component parts do DNA and RNA differs? [16]

2. Distinguish between unidirectional and bidirectional DNA synthesis. [16]

3. What are the 3 general mechanisms used in RNA splicing? Explain each of them.
[16]

4. Describe translation process in eukaryotic cells. [16]

5. What are mutation caused by the radiations like X-ray and UV-rays. Why is the
X-radiation is more potent mutagen than UV-radiation? [16]

6. A point mutation occurs in a particular gene. Describe the types of mutational


events that can restore a functional protein, including intergenic events. Consider
missense, nonsense, and frameshift mutation. [16]

7. Describe the basis for chromosome mapping in the Hf r × F − crosses. [16]

8. How T4 infects to bacteria and reproduction takes place. [16]

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Code No: RR222305 Set No. 2
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Sketch the shape of B-DNA and Z-DNA and write their properties. [16]

2. What is the start site for DNA replication? [16]

3. What are the general principles underlying all splicing mechanisms? [16]

4. Draw and describe the primary structure and secondary structure of protein and
their significance. [16]

5. Write short notes on

(a) Thymine dimers


(b) Error prone Repair [16]

6. A point mutation occurs in a particular gene. Describe the types of mutational


events that can restore a functional protein, including intergenic events. Consider
missense, nonsense, and frameshift mutation. [16]

7. Describe the mechanism of transformation process. [16]

8. Illustrate with diagrams and written designations a model for the life cycle of a
temperate (lysogenic) virus such as E. coli phage lambda (l). What is the role of
the lambda repressor protein in the lambda infection process? [16]

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Code No: RR222305 Set No. 3
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. If the tetranucleotide hypothesis were correct regarding the simplicity of DNA


structure, under what circumstances could DNA be the genetic material. [16]

2. Summarize and compare the properties of DNA polymerase I, II and III. [16]

3. How does the structure of a eukaryotic CoreRNAPol compare with


the E. coli CoreRNApol? [16]

4. Write a note on post-transnational modification. [16]

5. Write short notes on

(a) Thymine dimers


(b) Error prone Repair [16]

6. Describe the Ames assay for screening potential environmental mutagens. Why is
it though that a compound that tests positively in the Ames assay may also be
carcinogenic? [16]

7. Distinguish among the three modes of recombination in bacteria. [16]

8. Why are IS elements sometimes referred to as selfish DNA? [16]

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Code No: RR222305 Set No. 4
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. What are the roles of RNA primers and Okazaki fragments during DNA replication?
[16]

2. Write a note on DNA replication mechanism found in E. coli. [16]

3. What processing events occur with prokaryotic mRNA? [16]

4. Indicate the role of cysteine in stabilizing the tertiary structure of secretory proteins.
[16]

5. What is meant by Reverse Genetics? How is useful? [16]

6. A point mutation occurs in a particular gene. Describe the types of mutational


events that can restore a functional protein, including intergenic events. Consider
missense, nonsense, and frameshift mutation. [16]

7. How do you map the chromosome in Hfr bacteria? [16]

8. Define plaque, lysogeny and prophage. [16]

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