Code No: RR222305

Set No. 1

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007 MOLECULAR BIOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. In what component parts do DNA and RNA differs? 2. Distinguish between unidirectional and bidirectional DNA synthesis. [16] [16]

3. What are the 3 general mechanisms used in RNA splicing? Explain each of them. [16] 4. Describe translation process in eukaryotic cells. [16]

5. What are mutation caused by the radiations like X-ray and UV-rays. Why is the X-radiation is more potent mutagen than UV-radiation? [16] 6. A point mutation occurs in a particular gene. Describe the types of mutational events that can restore a functional protein, including intergenic events. Consider missense, nonsense, and frameshift mutation. [16] 7. Describe the basis for chromosome mapping in the Hf r × F − crosses. 8. How T4 infects to bacteria and reproduction takes place. ⋆⋆⋆⋆⋆ [16] [16]

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Code No: RR222305

Set No. 2

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007 MOLECULAR BIOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Sketch the shape of B-DNA and Z-DNA and write their properties. 2. What is the start site for DNA replication? 3. What are the general principles underlying all splicing mechanisms? [16] [16] [16]

4. Draw and describe the primary structure and secondary structure of protein and their significance. [16] 5. Write short notes on (a) Thymine dimers (b) Error prone Repair [16]

6. A point mutation occurs in a particular gene. Describe the types of mutational events that can restore a functional protein, including intergenic events. Consider missense, nonsense, and frameshift mutation. [16] 7. Describe the mechanism of transformation process. [16]

8. Illustrate with diagrams and written designations a model for the life cycle of a temperate (lysogenic) virus such as E. coli phage lambda (l). What is the role of the lambda repressor protein in the lambda infection process? [16] ⋆⋆⋆⋆⋆

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Code No: RR222305

Set No. 3

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007 MOLECULAR BIOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. If the tetranucleotide hypothesis were correct regarding the simplicity of DNA structure, under what circumstances could DNA be the genetic material. [16] 2. Summarize and compare the properties of DNA polymerase I, II and III. 3. How does the structure of a eukaryotic CoreRNAPol compare with the E. coli CoreRNApol? 4. Write a note on post-transnational modification. 5. Write short notes on (a) Thymine dimers (b) Error prone Repair [16] [16] [16] [16]

6. Describe the Ames assay for screening potential environmental mutagens. Why is it though that a compound that tests positively in the Ames assay may also be carcinogenic? [16] 7. Distinguish among the three modes of recombination in bacteria. 8. Why are IS elements sometimes referred to as selfish DNA? ⋆⋆⋆⋆⋆ [16] [16]

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Code No: RR222305

Set No. 4

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007 MOLECULAR BIOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. What are the roles of RNA primers and Okazaki fragments during DNA replication? [16] 2. Write a note on DNA replication mechanism found in E. coli. 3. What processing events occur with prokaryotic mRNA? [16] [16]

4. Indicate the role of cysteine in stabilizing the tertiary structure of secretory proteins. [16] 5. What is meant by Reverse Genetics? How is useful? [16]

6. A point mutation occurs in a particular gene. Describe the types of mutational events that can restore a functional protein, including intergenic events. Consider missense, nonsense, and frameshift mutation. [16] 7. How do you map the chromosome in Hfr bacteria? 8. Define plaque, lysogeny and prophage. ⋆⋆⋆⋆⋆ [16] [16]

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