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COURSE TITLE: COURSE NUMBER: SEMESTER: PONDERATION: INSTRUCTOR(S): Microbiology 101-301-VA A13 4h theory, 2h lab every other week, 3h homework Section Fred Nestel Terry Saropoulos Maria Panzuto OFFICE HOURS: 1 2 Office and extension A-428, 7656 A-430, 7661 A-422 7343

3 As posted on office door

COURSE OBJECTIVES This course includes an introduction to the field of microbiology, the relationships that exist between humans and microorganisms, the microbiological characteristics of pathogens, antimicrobial controls, immunity and the epidemiology of bacterial, viral, fungal, protozoal and parasitic infections. COURSE CONTENT The following is a tentative schedule of topics to be covered in the course. Further details relating to course content, learning objectives, reading and assignments will be announced and/or distributed as the course progresses.

TOPICS Unit one: Anatomy and Physiology of Microorganisms The microbial world and humans The prokaryotic and eukaryotic cells. Bacterial metabolism and growth. The viruses: structure and multiplication. Bacterial and viral genetics: conjugation, transformation, transduction, recombination. 6. Bacteria, fungi, protozoa and human multicellular parasites. (To be seen in part in Unit Four.) 7. Methods of studying microbes: visualization, cultivation, biochemical tests, animal inoculation. 8. Specimen collection in hospitals.


1. 2. 3. 4. 5.

1.1 1.1 1.1 1.1 1.1 1.1 1.1, 4.2 1.2, 5

1 4 5, 6 13 8, 9 5, 11, 12 3, 6, 10 Appendix C

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Unit two: Control of microbial growth to preclude diseases

1. 2. 3. 4.

Physical methods of microbial control. (Chapter 7) Chemical methods of microbial control. (Chapter 7) Practical techniques in microbial control. (Chapter 7) Antimicrobial drugs for human patient. (Chapter 20)

5 5 5 5

7 7 7 20

Unit three: Interaction between microorganisms and human hosts Principles of disease and epidemiology. Mechanisms of pathogenicity of microorganisms. Nonspecific defenses of the human host. Specific defenses of the human host: Humeral and cell-mediated acquired immunity. 5. Practical applications of immunology: Vaccines and diagnostic immunology. 6. Disorders associated with the immune system: hypersensitivity, autoimmunity, transplantation, immune deficiencies, AIDS, cancer.

1. 2. 3. 4.

1.2 1.3 2.1, 2.2, 2.3, 2.4 2.1, 2.2, 2.3, 2.4 4 3.1, 3.2, 3.3

14 15 16 17 18 19

Unit four: Microorganisms and human disease

1. 2. 3. 4. 5. 6.

Some diseases of the skin and eyes. Some diseases of the nervous system. Some diseases of the cardiovascular system and lymphatic system. Some diseases of the respiratory system. Some diseases of the digestive system. Some diseases of the urinary and reproductive systems.

3.4 3.4 3.4 3.4 3.4 3.4

21 22 23 24 25 26

REQUIRED TEXT Tortora, G.J., Funke, B.R., Case, C.L., Microbiology: An Introduction (11th edition) 2013, Benjamin Cummings, Addison Wesley Longman, Inc. (Approx. price $93 + tax at Vanier Bookstore) ISBN: 9780321796677 INSTRUCTIONAL FORMAT The course is conducted in two periods of lectures per week of 120 minutes each for all students, and one laboratory period of 120 minutes every second week. Sometimes the laboratory periods may be used as a lecture period for all. AV materials and laboratory demonstrations will be used when appropriate. The Biology Resource Centre (A-473) is set aside as a study area. Students are encouraged to ask questions and to contribute to lecture discussions as well as to arrange appointments with the instructor to discuss the subject matter, grades or any other problems that may arise. ATTENDANCE Attendance at lectures is not compulsory except for those lectures that include a laboratory period.
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Attendance will be taken for labs. Attendance at laboratories is compulsory and some laboratory periods will be continued in the lecture periods. In cases of unusual illness if a student has a medical note, the instructor should be informed as soon as possible and a make-up test could be arranged for a missed lab quiz. Students will be permitted to write lab quizzes only for those labs that they have performed. LABORATORY All labs will be held in A-443. Lab coats are compulsory for the microbiology labs. Eating and drinking (including water) is prohibited in the laboratory. Students will be split into Groups A & B and will attend labs every second week unless indicated otherwise in the schedule below. WITHDRAWAL If you should decide to drop this course prior to the official deadline for withdrawal (Thursday, September 19, 2013, 4:30pm), you must officially withdraw at the Registrars Office (H-100), not merely tell the instructor. Should you drop the course after the official withdrawal date, you will be assigned a numerical grade consisting of the marks accrued up to the date of withdrawal. EVALUATION Students will be evaluated on the basis of exams and quizzes as follows: Exam I: Unit I Exam II: Unit II Exam III: Unit III Exam IV: Unit IV Six (6) Lab Quizzes (6% each) 16% 16% 16% 16% 36%

Unit tests consist of objective questions usually of the multiple-choice type. Lab quizzes are short answer. Lab quizzes cover material from the previous lab. A student who has not performed a lab will not be permitted to write the lab quiz associated with that lab. The duration of the tests is at the discretion of the instructor and unless otherwise specified, the student is responsible only for the material covered in that unit or lab. The instructor will make announcements of unit tests and quizzes at least one week in advance. A student who fails to take a unit test or quiz will receive a zero grade for the missed test or quiz. Only in exceptional cases, e.g., sickness (with a physicians note), will it be possible to make up a missed test or quiz at a date determined by the instructor (usually at the end of the term). CHANGE OF GRADE REQUESTS A student who wishes to have his/her final grade reviewed will be given a maximum of fifteen (15) days after the receipt of his/her transcript in which to make his/her request. A Mark Update Request form should be submitted through the Registrars Office. Any further appeal, such as a Grades Review Request, should be in writing and submitted to the Biology Departments Student-Faculty Mediation Committee.

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COURSE POLICIES General Academic Policies: It is the student's responsibility to be familiar with and adhere to the Vanier College Academic Policies. These policies can be found online under Academic Policies at ). Your attention is drawn in particular to the following policies. A brief summary of each is included: Student Academic Complaints (Policy number 7210-8): The Vanier College Student Academic Complaints Policy and procedures puts an emphasis on mediation as the primary means to resolve complaints in the academic area. If you have a problem with a teacher and have been unable to resolve it by talking with him or her, you may wish to enlist the help of the Faculty Mediation Committee. The committee member names and contact information are available in Student Services or through the office of the Faculty Dean. The complete version of this policy is available online ( Cheating and Plagiarism (Policy number 7210-31): Any form of cheating or plagiarism will result in a grade of zero on the test or assignment and a letter from the teacher will be placed in your file. A repeated offence may lead to even more serious consequences. Please consult the Vanier Student Writing Guide, the Vanier College Catalogue, the Student Handbook, the policy online ( and your teacher for more information. Student Misconduct in the Classroom (Policy number 7210-19): This policy provides guidelines for handling cases of student misbehaviour in the classroom and other instructional settings. Such cases may include conduct that is abusive to the teacher and/or other students, or disruptive to the teaching/learning process. This policy does not limit the teachers or the Colleges right to take imm ediate action in cases of imminent danger to persons or property. The complete version of this policy is available online ( Zero Tolerance (Policy number 7110-2): The following disruptive behaviours will not be tolerated in any degree on campus: Unauthorized Use of Alcohol/Illegal Drugs; Violence against Persons or Property; Possession of Weapons; Verbal or Written Abuse or Intimidation; Theft or Gambling. The complete version of this policy is available online ( Student Absences for Religious Holy Days (Policy number 7210-20): Students whose religious obligations require them to be absent from the College on a holy day not formally recognized in the College calendar must inform their teachers, in writing, during the first week of classes, of the particular date(s) and times of the religious holy days on which they must be absent. Absences approved in this manner are considered to be excused absences. Students are responsible for material covered in the classes and labs them miss. The complete version of this policy is available online (

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OBJECTIVES / STANDARD (Performance) Element 1 To specify the action of an aggressive agent on the human body Performance Criteria 1.1 Identification of characteristics of an aggressive agent. 1.2 Consideration of method of transmission. 1.3 Precise description of the agents pathogenicity. LEARNING OUTCOMES To specify the action of an aggressive agent on the human body, the student will:

1. Describe the classification of microorganisms:

bacteria, fungi, protozoa, helminthes, viruses and prions. 2. Describe the structural and functional characteristics of prokaryotic and eukaryotic cells and viruses; microbial metabolism (biochemical identification tests); microbial growth requirements, culture media; microbial genetics (mutation, genetic transfer and recombination). 3. Describe the principle of diseases and epidemiology (normal microbiota; etiology of infectious diseases; development of diseases; spread of infection, reservoirs of infection; nosocom infections; epidemiology. 4. Describe the microbial mechanisms of pathogenicity (portal of entry; capsules, cell wall, enzymes, toxins of bacteria; pathogenetic properties of nonbacterial microorganisms, virus, fungi, protozoa and helminthes. 5. Perform some experiments in the following areas: Microscopy and staining methods Identification tests Cultivation of bacteria Metabolism Microorganisms and diseases Epidemiology To define the bodys reactions to the aggressor, the student will:

Element 2 To define the bodys reactions to the aggressor. Performance Criteria 2.1 Demonstration of bodys resistance to the aggressor. 2.2 Identification of conditions that are favourable or unfavourable to homeostasis. 2.3 Identification of disrupted physiological and metabolic functions. 2.4 Consideration of the evolving nature of the biological dysfunction.

1. Describe the non-specific defenses of the host; nonacquired immunity, mechanical, chemical and biological factors. 2. Describe the specific defenses of the host; acquired immunity (B-cells and humoral immunity: antigen and antibody; T-cells and cell-mediated immunity).

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OBJECTIVES / STANDARD (Performance) Element 3 To describe the infectious or immunological disorder. Performance Criteria 3.1 Precise determination of the disorders stages of development. 3.2 Identification of the disorders physiological process. 3.3 Accurate description of physiological and metabolic signs. 3.4 Identification of the epidemiological characteristics of the disorder. Element 4 To relate the immunopathological disorder to the therapy. Performance Criteria 4.1 Consideration of the physiological and metabolic manifestations. 4.2 Identification of diagnostic tests. 4.3 Consideration of the type of aggressive agent. 4.5 Demonstrations of principles underlying therapy.

LEARNING OUTCOMES To describe the infectious or immunological disorder, the student will:

1. Describe the disorders associated with the immune

system: hypersensitivity, autoimmune diseases and congenital and acquired immune deficiencies. 2. Describe some microbial disorders of: skin and eyes, nervous system, cardiovascular and lymphatic system, respiratory system, digestive system, urinary and reproductive system.

To relate the immunopathological disorder to the therapy, the student will:

1. Describe the practical application of immunology

(vaccines and immune sera and diagnostic immunology or tests). 2. Describe the antibiotic therapy of some microbial disorders. 3. Perform some experiments in the following areas: Immunology Diagnostic tests To examine measures to prevent or control infection, the student will:

Element 5 To examine measures to prevent or control infection. Performance Criteria 5.1 Identification of infectious process. 5.2 Judicious detection of contamination risks. 5.3 Identification of barriers required to prevent and control infection. 5.4 Consideration of universal precautions. 5.5 Consideration of work rules and procedures applicable to sterile and contaminated environments. 5.6 Consideration of safety measures applicable to the safe handling and disposal of biomedical waste.

1. Describe the control of microbial growth, i.e., physical

methods (heat, filtration, desiccation, osmotic pressure and radiation); chemical methods (disinfections and antisepsis); and antimicrobial drugs (chemotherapeutic agents). Describe the methods for taking clinical samples. Demonstrate the correct method for specimen collection. Demonstrate some measure to control microbial growth. Perform some experiments in the following areas: Control of microbial growth Collection of specimens

2. 3. 4. 5.

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LAB Week of Aug 26 Sept 2 Groups A & B No Laboratory FOR ALL STUDENTS Lab Intro Culture Media Measurement of Microbial Growth Demonstration of Enterotube Study of Prepared Slides Use of the Hanging Drop - Demonstration Simple Stains Gram Stain Acid Fast Stain Negative Staining for Capsules Spore or Endospore Staining Identification of an Unknown Bacteria An Experimental Epidemic Differential Identification of Enterobacteriaceae Effective of Ultraviolet light on Bacterial Growth Effect of Pasteurization on Bacterial Growth Effect of Salt Concentration on Bacterial Growth Microscopic Observation of Yogurt Bacteria on the Human Body Bacteria at Specific Sites in Our Surroundings Susceptibility of Bacteria to Antibiotics Evaluation of Antiseptics Test of Effectiveness of Methods for Disinfecting Hands Bacteria of the Mouth (Snyders Test) Dec 2 Groups A & B Review ACTIVITIES TEXTBOOK (page #)

164-170 174-179 282-294 55-73

Sept 9 Group A Sept 16 Group B Sept 23 Group A Sept 30 Group B Oct. 7 Group A Oct 14 Group B Oct 21 Group A Oct 28 Group B Nov 4 Group A Nov 11 Group B

69-70, 85-88 87-88 71 71 282-294 409-416 282-294 192-194 190-191 159, 192 797-800 Appendix C on AP-8 710-712 572-573 195-202 185-186 706-709 431-432

Nov 18 Goup A Nov 25 Group B

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