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Sophia Spadavecchia

sophia.spada@gmail.com
EDUCATION University of Medicine and Dentistry of New Jersey PhD, Biomedical Sciences Dissertation: Kaposi’s Sarcoma-associated Herpesvirus lytic transactivators in pathogenesis. Montclair State University BS, cum laude, Molecular Biology Minor in chemistry. RESEARCH EXPERIENCE Public Health Research Institute Newark, NJ Postdoctoral researcher; Advisor: David Dubnau 2010-2013 MecA regulation of fatty acid metabolism in Bacillus subtilis  Aim: To identify the mechanism of MecA regulation of fatty acid synthesis using biochemical approaches.  Analyze changes in biotin carboxylase protein expression, complex formation and enzymatic activity in the absence and over-expression of MecA.  Utilized western blotting, tagged protein purifications, EMSA, ATP hydrolysis coupled assay, GST pull-downs, FPLC gel filtration, co-immunoprecipitation. University of Medicine and Dentistry of New Jersey Newark, NJ Doctoral researcher; Advisor: David M. Lukac 2003-2010 Kaposi's Sarcoma-Associated Herpesvirus regulation of Epstein-Barr Virus oncogenes and cell growth requires viral and cellular proteins.  Aim: Identify host and viral factors required for Kasposi’s sarcoma-associated herpesvirus pathogenesis in the context of a co-infection with Epstein Barr Virus  Demonstrated KSHV Rta transactivates EBV oncogenes via the cellular RBP-Jκ and is required for short-term cellular growth.  Utilized luciferase promoter fusions, immunofluorence, antibody arrays, cytotoxicity assays, qRT-PCR, and EMSAs. Newark, NJ 2010

Montclair, NJ 2003

GRANTS New Jersey Commission on Cancer Research Pre-Doctoral Fellowship University of Medicine and Dentistry of New Jersey 2005-2007

RESEARCH FELLOWSHIPS Howard Hughes Medical Institute, Villanova University Summer Undergraduate Research Fellowship Waksman Institute of Microbiology, Rutgers University Summer Student Scholar’s Program, Onion Genome Project Philadelphia, PA 2002 Piscataway, NJ 1998

PEER-REVIEWED PUBLICATIONS Prepiak P.*, Defrancesco M.*, Spadavecchia S., Mirouze N., Albano M., Persuh M., Fujita M., Dubnau D. MecA dampens transitions to spore, biofilm exopolysaccharide and competence expression by two different mechanisms. Mol Microbiol.;80(4):1014-30 (2011) Spadavecchia S., Gonzalez-Lopez O, Carroll KD., Palmeri D., Lukac DM. Convergence of Kaposi's sarcoma-associated herpesvirus reactivation with Epstein-Barr virus latency and cellular growth mediated by the notch signaling pathway in coinfected cells. J Virol 84(20):10488-50 (2010) Palmeri D., Spadavecchia S., Carroll KD., Lukac DM. Promoter- and cell-specific transcriptional transactivation by the Kaposi's sarcoma-associated herpesvirus ORF57/Mta protein. J Virol. 81(24):13299-314 (2007). Carroll KD., Khadim F., Spadavecchia S., Palmeri D., Lukac DM. Direct interactions of Kaposi's sarcoma-associated herpesvirus/human herpesvirus 8 ORF50/Rta protein with the cellular protein octamer-1 and DNA are critical for specifying transactivation of a delayed-early promoter and stimulating viral reactivation. J Virol. 81(16):8451-67 (2007). Carroll KD., Bu W., Palmeri D., Spadavecchia S., Lynch SJ., Marras SA., Tyagi S., Lukac DM. Kaposi's Sarcoma-associated herpesvirus lytic switch protein stimulates DNA binding of RBPJk/CSL to activate the Notch pathway. J Virol. 80(19):9697-709 (2006).

PRESENTATIONS Spadavecchia S., Driscoll KD., Palmeri D., and Lukac DM. (2009). Convergence of Kaposi's sarcoma-associated herpesvirus reactivation with Epstein-Barr virus latency by the notch signaling pathway in coinfected cells. Poster presentation at the 34th Annual International Herpesvirus Workshop, Cornell University, Ithaca, New York USA. Spadavecchia S., Driscoll KD., Palmeri D., and Lukac DM. (2007) Host cell control of cell and promoter specific transactivation by the KSHV ORF57/Mta protein. Poster at the Annual Retreat on Cancer Research. Robert Wood Johnson Medical School, Piscataway, NJ. *Award

Spadavecchia S.*, Palmeri D.*, Lukac DM. (2006) Cell and promoter-specific transactivation by the KSHV ORF57/Mta protein. Oral presentation at the 9th Annual Workshop on Kaposi’s Sarcoma-Associated Herpesvirus (KSHV) and Related Agents. Cape Cod, MA. Spadavecchia S.*, Palmeri D.*, Lukac DM. (2006) Cell and promoter-specific transactivation by the KSHV ORF57/Mta protein. Poster presentation at the Annual Retreat on Cancer Research. Robert Wood Johnson Medical School, Piscataway, NJ. Spadavecchia S.*, Palmeri D.*, Lukac DM. (2005) Cell and promoter-specific transactivation by the KSHV ORF57/Mta protein. Poster presentation at the 9th International Conference on Malignancies in AIDS and Other Acquired Immunodeficiencies. North Bethesda, MD.

AWARDS New Jersey Cancer Research Award for Scientific Excellence (Poster Presentation) Edward J. Bloustein Scholar St. Peter's College Summer Student Scholar 2007 1999 1997

ORGANIZATIONS American Society for Microbiology New York Academy of Sciences Golden Key International Honor Society

WORK EXPERIENCE Wilson Ornithological Society Editorial Assistant, The Wilson Bulletin  Filed and organized all received manuscripts for efficient processing.  Searched for appropriate reviewers.  Corresponded with authors and reviewers.  Proofread and formatted accepted manuscripts. Montclair, NJ 2001-2003

TEACHING EXPERIENCE University of Medicine and Dentistry of New Jersey Mentor, Lukac Laboratory  Trained and supervised rotating graduate students, Master's thesis students, medical students, and a pre-med student. New Jersey Medical School Laboratory Instructor, Medical Microbiology Newark, NJ 2006-2009

Newark, NJ Spring 2005

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Introduced contents and practicals for each laboratory Supervised students during each laboratory session.

Spring 2006

Montclair State University Teaching Assistant, Genetics Laboratory  Supervised and assisted students experiments and semester projects.  Prepared materials necessary for each laboratory session.  Proofread final lab reports; tutored students needing extra help. Teaching Assistant, Principles of Biology I Laboratory  Supervised and assisted students performing experiments. Teaching Assistant, Microbiology Laboratory  Supervised and assisted students performing experiments in class and for semester projects.

Montclair, NJ 2000-2003

Spring 2002

Spring 2000

TRAINING GE Biacore Basics  Biacore 2000 instrument operation and routine maintenance.  Designed affinity and kinetic experiments.  Evaluated data with BIAevaluation software. 2010