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"Active Potassium Transport Across Guinea-Pig Distal Colon: Action of S" by Gerhard Rechkemmer, et al.

SelectedWorks of Dan R. Halm
DAN R. HALM
Wright State Univ ersity Associate Professor, Department of Neuroscience, Cell Biology & Physiology

Active Potassium Transport Across Guinea-Pig Distal Colon: Action of Secretagogues
Gerhard Rechkemmer Raymond A. Frizzell Dan R. Halm, Wright State University - Main Campus Abstract 1. Adrenaline (5 uM) stimulated a K+ secretory current by 2-2 ftequiv h-' cm2 in isolated guinea-pig distal colonic epithelium. This secretory activity was inhibited entirely by addition of the loop diuretic bumetanide to the serosal solution. On-going K+ uptake via the absorptive pathway was unaltered by these changes. 2. Prostaglandin E2 (PGE2, 2 /M) stimulated electrogenic K+ secretion and Cl- secretion by 3 0 and 3-6 ,tequiv hcmM2, respectively. Serosal addition of bumetanide completely inhibited this K+ secretion but blocked only -70% of Cl- secretion. The bumetanideinsensitive Cl- secretory current was dependent on the presence of Cl- and HC03- in the bathing solutions. 3. Stimulation of electrogenic K+ secretion by PGE2 occurred with a half-maximal concentration of 4 nm, an affinity -300 times higher than that for stimulation of Clsecretion by PGE2. 4. Forskolin (10 AM) stimulated Cl- secretion by 4-9 tequiv h-' cm2. The apparent K+ secretory rate was increased by only 1-5 ytequiv h-1 cm2. A bumetanide-insensitive shortcircuit current (Isc) was apparent and of the same size as that stimulated by PGE2. 5. Addition of the Ca2+ ionophore A23187 (10 fSM), in the presence of indomethacin (1 /SM) to reduce prostaglandin production, inhibited the K+ absorptive pathway by 40% and concurrently stimulated a small rate of electrogenic K+ secretion. 6. Active K+ absorption was inhibited by the addition of ouabain, omeprazole or SCH28080 to the mucosal solution. Both omeprazole and SCH28080 also stimulated a small negative Isc, consistent with electrogenic K+ secretion. 7. Association of K+ absorption, K+ secretion and Cl- secretion is indicated by similarities in transport mechanism and by secretagogue regulation. In particular, maximal rates of K+ secretory current require uptake via apical membrane K+ pumps. Such interrelations support a common cellular locus for these ion transport pathways. Suggested Citation Gerhard Rechkemmer, Raymond A. Frizzell, and Dan R. Halm. "Active Potassium Transport Across Guinea-Pig Distal Colon: Action of Secretagogues" The Journal of Physiology 493.2 (1996): 485-502. Available at: http://works.bepress.com/dan_halm/17 Link to Full Text Find in your library Share Share Share Share Share More

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