Code No: R050212305

Set No. 1

II B.Tech I Semester Supplementary Examinations, February 2007 MICROBIOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Discuss the discoveries of Redi, Needham, Spallanzani, and Ferdinand in disproving the theory of spontaneous generation. [16] 2. Briefly describe the endospore formation and germination in Bacteria. 3. Describe the viruses of eucaryotic organisms. 4. How does a bacteriophage introduce its nucleic acid into host cell. [16] [16] [16]

5. Briefly describe in general terms the morphology and nucleic acids of the major phage types. [16] 6. Write short notes on: (a) Growth factors. (b) Aerobes. (c) Anaerobes. (d) Nutrients. 7. Write short notes on: (a) Spore preparation. (b) Lyophilisation. (c) Cryopreservation. (d) Stock cultures. [4+4+4+4] [4+4+4+4]

8. What are the chemotherapeutic agents? Discuss the effectiveness of chemotherapeutic agents. [16] ⋆⋆⋆⋆⋆

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Code No: R050212305

Set No. 2

II B.Tech I Semester Supplementary Examinations, February 2007 MICROBIOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Describe the contributions of the following people in the development of microbiology a) John Tyndall b) Alexander Fleming c) John Needham d) Francisco Redi. [4+4+4+4] 2. How PCR technique is useful in classifying bacteria. 3. Mention the difference between animal and plant viruses. 4. Describe the replication and transcription in RNA viruses with example. 5. What is a virus? Discuss various types of viral infections. 6. Write short notes on: (a) Growth factors. (b) Aerobes. (c) Anaerobes. (d) Nutrients. [4+4+4+4] [16] [16] [16] [16]

7. Define water activity and briefly discuss how it can be determined. Why is it diffcult for microorganisms to grow at low water activity (aw) values. [16] 8. Describe surfactants, alkylating agents and heavy metals in terms of their chemical nature as disinfectants. [16] ⋆⋆⋆⋆⋆

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Code No: R050212305

Set No. 3

II B.Tech I Semester Supplementary Examinations, February 2007 MICROBIOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. What are the different serological methods of identification of microorganisms?[16] 2. Write notes on: (a) FAME (b) Ribotyping (c) Bacterial Speciation. 3. Describe the structural properties of virus. 4. Discuss general steps in viral replication. 5. What is a virus? Discuss various types of viral infections. 6. What are nutrients? Describe major nutritional types of microorganisms. [6+4+6] [16] [16] [16] [16]

7. Explain the influence of environmental factors on growth of microorganisms. [16] 8. Explain various methods to control microorganisms growth. ⋆⋆⋆⋆⋆ [16]

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Code No: R050212305

Set No. 4

II B.Tech I Semester Supplementary Examinations, February 2007 MICROBIOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Describe the contributions of the following people in the development of microbiology a) John Tyndall b) Alexander Fleming c) John Needham d) Francisco Redi. [4+4+4+4] 2. Describe the eucaryotic diversity with suitable examples. 3. What is ‘hepatitis’ ? Explain about hepatitis viruses. 4. Explain the entry of viruses into host cells. [16] [16] [16]

5. How is a one step growth experiment carried out ? Summarize what occurs in each phase of the resulting growth curve. Define latent period, eclipse period, rise period and burst size. [16] 6. What are nutrients? Describe major nutritional types of microorganisms. [16]

7. Write the effect of the following environmental factors on the growth of microorganisms. (a) Solutes. (b) PH (c) Oxygen (d) Osmotic pressure. [4+4+4+4] [16]

8. Explain the use of physical methods in control of microorganisms growth. ⋆⋆⋆⋆⋆

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