Code No: RR312302

Set No. 1

III B.Tech I Semester Supplementary Examinations, February 2007 GENETIC ENGINEERING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. What is attenuation? How does it help in controlling gene expression? 2. Explain the expression of hormone regulated genes in eukaryotes 3. Explain the structure of PBR322. 4. Write notes on any two: (a) Ploylinker DNA (b) Staggered cuts (c) λ - Phage as vector 5. What are probes? Discuss their importance in cloned gene detection. [8+8] [4+12] [4+12] [6+10] [4+12]

6. What is the basic principle of PCR? Write about the different types of PCR. [6+10] 7. Comment on the applications of molecular markers in various fields of biotechnology. [4+12] 8. Explain the detailed structure of a Ti plasmid in Agrobacterium. ⋆⋆⋆⋆⋆ [6+10]

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Code No: RR312302

Set No. 2

III B.Tech I Semester Supplementary Examinations, February 2007 GENETIC ENGINEERING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Write notes on any two (a) CAP- binding site (b) Translation regulation (c) Regulator 2. Write notes on any two: (a) Steriod hormonal regulation (b) Short repeats in DNA (c) DNA binding motifs. [8+8] [8+8]

3. What are the characters of ideal plasmid? Explain with suitable example. [8+8] 4. Write notes on any two: (a) Restriction digestion (b) Homopolymer tailing (c) Ligases 5. Write the steps involved in construction of a genomic library. 6. Explain the role of PCR in Forensic Science. [8+8] [4+12] [4+12]

7. Comment on the applications of molecular markers in various fields of biotechnology. [4+12] 8. Describe the construction of plant vectors based on Agrobacterium Ti plasmid. [6+10] ⋆⋆⋆⋆⋆

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Code No: RR312302

Set No. 3

III B.Tech I Semester Supplementary Examinations, February 2007 GENETIC ENGINEERING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Write about Arabinose operon regulation in Prokaryotes. 2. Write notes on any two: (a) Tissue specific enhancers (b) Phosphorylated proteins (c) TATA box 3. Write notes on any two: (a) Relaxed plasmid (b) PUC8 (c) α - Complementation. 4. Write detailed account on restriction mapping and its importance. 5. Explain nucleic acid hybridization and its uses as a screening procedure. [8+8] [4+12] [6+10] [8+8] [4+12]

6. What is the basic principle of PCR? Write about the different types of PCR. [6+10] 7. What is gene mapping? Explain the role of restriction enzymes in it. [6+10]

8. Elaborate on the importance of transgenic animals citing suitable examples. [8+8] ⋆⋆⋆⋆⋆

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Code No: RR312302

Set No. 4

III B.Tech I Semester Supplementary Examinations, February 2007 GENETIC ENGINEERING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Differentiate between positive and negative control in lac operon. 2. Write notes on any two: (a) Silencers (b) Transacting regulatory proteins (c) Britton and Davidson model 3. Write notes on any two: (a) Mechanism of transpositions (b) Isolation of plasmids (c) Ty elements in yeast. [8+8] [8+8] [8+8]

4. Give the methods of isolation and purification of DNA from plant systems. [6+10] 5. Give a brief definition of a gene library. What is the essential difference between a genomic library and a cDNA library? List the major advantages/limitations on the use of each. [8+8] 6. Discuss about Multiplex PCR. 7. Write short notes on any two: (a) Satellite markers (b) Restriction enzymes (c) Gel electrophoresis. 8. Discuss about the viral methods used in doing In vivo gene therapy. ⋆⋆⋆⋆⋆ [8+8] [4+12] [4+12]

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