Code No: RR312304

Set No. 1

III B.Tech I Semester Supplementary Examinations, February 2007 PLANT BIO-TECHNOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Explain about direct and indirect embryogenesis and significance of somatic embryogenesis in plant tissue culture? [8+8] 2. Explain the process of production of secondary metabolites in culture? 3. What are methods of augmenting cell biomass in bioreactors? 4. Write short notes on any two (a) Vir gene (b) T- DNA transfer (c) Selectable marker 5. How transgenic plants are produced using various viruses as vectors? [16] [16] [16] [8+8]

6. What are ferredoxin and flavodoxin and give an account on the role of them in Nitrogen fixation? [3+3+10] 7. What are transposons? Discuss their role in genetic engineering. 8. Explain the process of gene transfer of herbicide tolerance. ⋆⋆⋆⋆⋆ [4+12] [16]

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Code No: RR312304

Set No. 2

III B.Tech I Semester Supplementary Examinations, February 2007 PLANT BIO-TECHNOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Describe historical view in the developmental aspect of plant tissue culture. [16] 2. What are elicitors? Give an account on a variety of physiological responses produced under chemically defined and fungal elicitor stress. [4+12] 3. Explain the factors effecting growth in a bioreactor? 4. What is meant by Agrobacterium mediated gene transfer? [16] [16]

5. Explain in detail about various viral vectors used for gene transfer in plants? [16] 6. What is meant by Symbiosis? Explain it with a suitable example. 7. Write a note on phenotypic and genotypic effects of transposition? 8. Write short notes on any two (a) Protinase inhibitors (b) Recombinant baculovirus (c) Insect control agents ⋆⋆⋆⋆⋆ [6+12] [8+8] [8+8]

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Code No: RR312304

Set No. 3

III B.Tech I Semester Supplementary Examinations, February 2007 PLANT BIO-TECHNOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Write a note on totipotency? Explain the importance of organogenesis? 2. Explain the process of production of secondary metabolites in culture? [6+10] [16]

3. What are the different bioreactors? Compare the functioning of different bioreactors? [8+8] 4. Explain about the importance of cloning vector in the Agrobacterium mediated gene transfer? [16] 5. Give an account on various viral vectors used in gene transfer? 6. Give an account on legume symbiosis. 7. Give an account on the transposons of Prokaryotes? [16] [16] [16]

8. Explain the role of Bt endotoxin genes in the introduction of insect control agents in to plants. [16] ⋆⋆⋆⋆⋆

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Code No: RR312304

Set No. 4

III B.Tech I Semester Supplementary Examinations, February 2007 PLANT BIO-TECHNOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Explain the importance of acclimatization process in plant biotechnology? How this can be achieved? [10+6] 2. List the secondary metabolites of plant origin performing as insecticides? 3. Bioreactors are boom for the production of secondary metabolites- justify. [16] [16]

4. What is meant by cocultivation? Explain its importance in transformation technique? [8+8] 5. What is the importance of Gemini viruses in the integration of foreign gene in to the plant host cell? [16] 6. What is meant by Symbiosis? Explain it with a suitable example. 7. What are the differences among an Is element and a transposon? 8. Explain the process of gene transfer of herbicide tolerance. ⋆⋆⋆⋆⋆ [6+12] [8+8] [16]

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