Code No: RR412302

Set No. 1

IV B.Tech I Semester Supplementary Examinations, February 2007 DOWN STREAM PROCESSING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. What are the various products of r DNA and their economic assessment of various proteins? [16] 2. Describe about primary factors affecting separation? 3. Write in detail about physico-mechnaical methods of Cell disruption? 4. Explain about Cell aggregation and Flocculation? 5. Write about different modes of operation of filters? 6. Explain the various factors that influence protein solubility? 7. Write short notes on: (a) Paper chromatography (b) SDS-PAGE 8. Explain about the process of Dialysis? ⋆⋆⋆⋆⋆ [8+8] [16] [16] [16] [16] [16] [16]

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Code No: RR412302

Set No. 2

IV B.Tech I Semester Supplementary Examinations, February 2007 DOWN STREAM PROCESSING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Describe about preliminary economic analysis or cost analysis performed in Bioprocess? [16] 2. Explain the role of Downstream processing and different market sectors in Biotechnology? [16] 3. Explain with a neat sketch the working of Homogenizer? 4. Explain about Cell aggregation and Flocculation? 5. Write short notes on: (a) Reverse Osmosis (b) Ultra filtration [8+8] [16] [16]

6. Describe the importance of process integration and whole broth treatment and in-situ recovery strategies for product recovery? [16] 7. Write short notes on: (a) Paper chromatography (b) SDS-PAGE 8. Explain about the process of Dialysis? ⋆⋆⋆⋆⋆ [8+8] [16]

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Code No: RR412302

Set No. 3

IV B.Tech I Semester Supplementary Examinations, February 2007 DOWN STREAM PROCESSING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Examine the continuing role which economics plays in Bioprocess research,development and commercialization? [16] 2. Explain the role of Downstream processing in Biotechnology? 3. Write in detail about physico-mechnaical methods of Cell disruption? [16] [16]

4. What are the various techniques employed in recovery of Bioproducts? Explain in brief about them? [16] 5. Write about different modes of operation of filters? 6. Discuss about Colloidal stability of protein solutions? 7. Write short notes on: (a) Rocket Immuno electrophoresis (b) Capillary electrophoresis 8. Write short notes on: (a) Secondary nucleation (b) Heterogeneous nucleation ⋆⋆⋆⋆⋆ [8+8] [8+8] [16] [16]

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Code No: RR412302

Set No. 4

IV B.Tech I Semester Supplementary Examinations, February 2007 DOWN STREAM PROCESSING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Examine the continuing role which economics plays in Bioprocess research,development and commercialization? [16] 2. Explain about the Filtration technique employed for several Bioproducts? 3. Write short notes on: (a) Homogenizer (b) Bead mill [8+8] [16]

4. What are the Factors influencing Sedimentation rates of particles in Batch gravity? [16] 5. How can RO and Ultra filtration complement each other? 6. Describe about different techniques employed in Precipitation? 7. Write short notes on: (a) Paper chromatography (b) SDS-PAGE [8+8] [16] [16]

8. Explain about the Principle and application of Gel Permeation chromatography? [16] ⋆⋆⋆⋆⋆

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