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LS23LSAFETYSHEETFORPROTEINS&SDSPAGELAB

YoumustreviewthissheetandtheattachedSOPscarefullybeforecomingtolab.Youareresponsibleforfollowing
allsafetyguidelineswhileinlabandyouwillbetestedonthismaterialintheprelabquiz.

STANDARDOPERATINGPROCEDURES(SOPS)REFERREDTOINTHISLAB:
TGSBuffer(1xconcentration)
iGels(TrisGlycinegelsmanufacturedbyNuSep)

POTENTIALHAZARDS:
Bothgelsandbuffercontainmaterialwhichcancauserespiratorytract,eyeandskinirritation.Containsmaterial
whichcausesdamagetothefollowingorgans:skin,eyes.Maybeharmfulifabsorbedthroughskinorifswallowed.
Maybeharmfulifinhaled.

ENGINEERINGANDWORKPRACTICECONTROLS:
Chemicalsshouldonlybeusedinlabrooms(YHS2336,2370,2379)

PERSONALPROTECTIVEEQUIPMENT(PPE):
Inadditiontostandardlabattire,youmustwear:
Safetyglassesorgoggleswhenworkingwithyourproteinsampleandloadingthegel
Gloveswhenhandlingyourproteinsampleandloadingthegel
Labcoatfortheentiretimeyouareinthelabroom

WASTEDISPOSAL:
Usedpipettetipsandmicrocentrifugetubesregulartrash
Microcentrifugetubescontainingsmallvolumeofproteinmixregulartrash
YourTAwillhandlethedisposalofthegelsandthebuffer

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StandardOperatingProcedure
forworkwith
Chemicalname:
iGelsbyNuSep
CAS#:
SeelistatendofSOP
PI:
Dr.GastonPfluegl
Updated: 3/28/2013
Building: YoungHallSouth
Room(s): YHS2344,2336,2370,2379

1. CircumstancesofUse/Storage:
iGelsareprecastpolyacrylamidegelsmanufacturedbyNuSepandstoredat4CinYHS2344.Thegelsarecarried
intothelabrooms(YHS2336,2370,2379)fortheseparationofproteinsintheproteinslabinLS23L.Gelsare
enclosedinaplasticcassette.ManufacturersdescriptionClear,solid,gelencasedinahardplasticcassette,
individuallypackagedinaplasticsachetinstoragebuffer(pH8.5).

2. PotentialHazards:
Inhalation:Thegelisunlikelytoposeaninhalationhazardbecauseitisencasedinaplasticcassette.
Skincontact:Datasuggeststhatthisproductshouldpresentnosignificantproblems.
Eyecontact:Thisproductmaybemildlyirritatingtoeyes.However,itisunlikelytocauseanymorethan
mildtransientdiscomfort.Itisalsounlikelytocauseanylastingeffects.
Ingestion:Ingestionofthecassetteisunlikely.Gelmaycausegastrointestinalirritationifswallowed.
ChronicExposure:Nospecificdataisavailablefortheproductforchronicexposuresymptoms.
CARCINOGENSTATUS:IngredientsarenotclassifiedashumancarcinogensbytheInternationalAgencyforResearch
onCancer(IARC).

3. EngineeringControls:
N/A

4. WorkPracticeControls:
iGelswillonlybeusedinthelabrooms(YHS2336,2370,2379).

5. Personalprotectiveequipment(PPE):
Allpersonnelarerequiredtowearthefollowingpersonalprotectiveequipmentwheneverhandlingthismaterial(in
additiontostandardlabattire):Safetyglassesorgoggles,gloves,labcoat.

6. TransportationandStorage:
iGelsarestoredinthepreproom(YHS2344)at4C.Individuallypackagedgelsaresentouttothelabrooms(YHS
2336,2370,2379)foruse.

7. WasteDisposal:
LSCorelabstaffisresponsibleforthesafecollection,preparationandproperdisposalofwaste.iGelsarenot
consideredtoxicandcanbedisposedofasnonhazardouswaste.

8. Exposures/Unintendedcontact:

UCLACampusPolice911(telldispatcheryouareatUCLAifcallingfromcell)or310.825.1491
EnvironmentalHealthandSafetyx59797(310.825.9797)
EmergencyRoom(RonaldReaganMedicalCenter)x52111(310.825.2111)

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CompletetheincidentreportfoundontheLSCoreLabsprepserverandnotifyEH&Sofanyseriousaccidentswithin
8hours(310.825.9797).

SkinContact
Datasuggeststhatthisproductshouldpresentnosignificantproblems.
EyeContact
Thisproductmaybemildlyirritatingtoeyes.However,itisunlikelytocauseanymorethanmildtransient
discomfort.Itisalsounlikelytocauseanylastingeffects.Quicklyandgentlyblotorbrushawaychemical.
Immediatelyflushthecontaminatedeye(s)withlukewarm,gentlyflowingwaterfor5minutes,oruntilthechemical
isremovedwhileholdingeyelidsopen.Ifirritationpersists,obtainmedicaladvice.
Inhalation
Thegelisunlikelytoposeaninhalationhazardbecauseitisencasedinaplasticcassette.
Ingestion
Ingestionofthecassetteisunlikely.Gelmaycausegastrointestinalirritationifswallowed.Drinkplentyofwaterand
seekimmediatemedicalattention.
NODATAONCHRONICEXPOSURE.INGREDIENTSNOTCLASSIFIEDASHUMANCARCINOGENS.

9. SpillProcedure:
AlllargespillsmustbereportedtoEH&S(310.825.9797)assoonaspossible.
N/A

10. AuthorizedPersonnel:
LSCoreLabStaff
LSCoreTAsandUAs(onlyafterTA/UAtrainingmeeting)
LSCoreStudents(onlyundersupervisionoftrainedTAs)

11. RequiredTraining:LSCoreLabStafftrainingshouldincludethefollowing:

ReviewofthecurrentMSDS
Reviewofthedepartmentalsafetyprocedures
ReviewoftheUCLAChemicalHygienePlan
CompletionoftheUCLALaboratorySafetyclassadministeredbyEH&S
CompletionofannualonlinerefresherLaboratorySafetyclassadministeredbyEH&S
CompletionofannualPPEtraining

12. Composition/InformationonIngredients:
IngredientsCASNo.Proportion,%
Polyacrylamide9003058<30*
TRIS77861<2.5
Sodiumazide*26628228<0.004**
Water7732185to100
*Averageconcentrationinmosthighlyconcentratedgel,othergelscontainless.
**Sodiumazideinstoragebufferonly.

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StandardOperatingProcedure
forworkwith
Chemicalname:
TGSbuffer(1xsolution)
CAS#:
SeelistatendofSOP
PI:
Dr.GastonPfluegl
Updated: 3/28/2013
Building: YoungHallSouth
Room(s): YHS2344,2336,2370,2379

13. CircumstancesofUse/Storage:
TGSbuffer10xconcentratewillbepurchasedin4Lcubesandstoredinthepreproom(YHS2344).Prepstaffwill
diluteto1xina10Lcarboy.Bottlesof1xTGS(two50mLbottlespersection)willbecarriedouttothelabrooms
(YHS2336,2370,2379)aspartoftheproteinsSDSPAGElabinLS23L.Usedbufferwillbedisposedofasdescribed
below.

14. PotentialHazards:
WARNING!Causesrespiratorytract,eyeandskinirritation.Containsmaterialwhichcausesdamagetothefollowing
organs:skin,eyes.Maybeharmfulifabsorbedthroughskinorifswallowed.Maybeharmfulifinhaled.

15. EngineeringControls:
N/A

16. WorkPracticeControls:
TGSbufferwillonlybeusedinthelabrooms(YHS2336,2370,2379)atthediluted1xconcentration.

17. Personalprotectiveequipment(PPE):
Allpersonnelarerequiredtowearthefollowingpersonalprotectiveequipmentwheneverhandlingthismaterial(in
additiontostandardlabattire):Safetyglassesorgoggles,gloves,labcoat.

18. TransportationandStorage:
TGS10xconcentrateisstoredinthepreproom(YHS2344).1xbottlesarecarriedtolabrooms(YHS2336,2370,
2379).Containersshouldbekepttightlycapped.

19. WasteDisposal:
PerEH&S,the1.0xTGSbufferusedintheLSCoreLabs,uncontaminatedandinapHrangeof6.08.0isapproved
fordraindisposal.Usedbuffershouldbepoureddownthesinkandflushedwithwater.
Thisisanexceptionforourlabsonlyanditshouldnotbeassumedthatotherbufferscanbedraindisposed.

20. Exposures/Unintendedcontact:

UCLACampusPolice911(telldispatcheryouareatUCLAifcallingfromcell)or310.825.1491
EnvironmentalHealthandSafetyx59797(310.825.9797)
EmergencyRoom(RonaldReaganMedicalCenter)x52111(310.825.2111)

CompletetheincidentreportfoundontheLSCoreLabsprepserverandnotifyEH&Sofanyseriousaccidentswithin
8hours(310.825.9797).
SkinContact
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Immediatelyflushskinwithplentyofwaterforatleast15minuteswhileremovingcontaminatedclothingand
shoes.Getimmediatemedicalattention.Washclothingbeforereuse.Thoroughlycleanshoesbeforereuse.Get
medicalattentionimmediately.
EyeContact
Immediatelyflusheyeswithplentyofwaterforatleast15minutes,liftingupperandlowereyelidsoccasionally.
Checkforandremovecontactlenses.Getmedicalattentionimmediately.
Inhalation
Removetofreshair.Ifnotbreathing,giveartificialrespiration.Ifbreathingisdifficult,giveoxygen.Getmedical
attention.
Ingestion
Ifswallowed,DONOTINDUCEVOMITING.Givesmallquantitiesofwatertoavoidinducingvomiting.Ifvomiting
occurs,theheadshouldbekeptlowsothevomitdoesnotenterthelungs.Nevergiveanythingbymouthtoan
unconsciousperson.Getmedicalattentionimmediately.

21. SpillProcedure:
AlllargespillsmustbereportedtoEH&S(310.825.9797)assoonaspossible.
Usepersonalprotectiveequipmentasdescribedabove.Coverandrecoverliquidwhenpossible.Collectliquidinan
appropriatecontainerorabsorbwithaninertmaterial(e.g.vermiculite,drysand,earth)fromthespillkitavailable
ineachroomandplaceinachemicalwastecontainerfordisposal.

22. AuthorizedPersonnel:
LSCoreLabStaff
LSCoreTAsandUAs(onlyafterTA/UAtrainingmeeting)
LSCoreStudents(onlyundersupervisionoftrainedTAs)

23. RequiredTraining:LSCoreLabStafftrainingshouldincludethefollowing:

ReviewofthecurrentMSDS
Reviewofthedepartmentalsafetyprocedures
ReviewoftheUCLAChemicalHygienePlan
CompletionoftheUCLALaboratorySafetyclassadministeredbyEH&S
CompletionofannualonlinerefresherLaboratorySafetyclassadministeredbyEH&S
CompletionofannualPPEtraining

24. Composition/InformationonIngredients:
NameCASnumber%byweight(at1xdilution)
Water773218598.298.1
Glycine564061.41.5
Tris(hydroxymethyl)aminomethane778610.3
SodiumDodecylSulfate1512130.1

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