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Drago Cobzariu, Stelian Britreanu, Doina Dane

Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, Romania Splaiul Independentei 105, 50097, 5th District, Bucharest, Romania;

Introduction Canine Parvovirus (CPV) continue to produce several outbreaks in Romania. Veterinary practitioners prescribe and breeders usually accept CPV vaccination. Despite these measures, some litters of puppies do not develop a protective response after vaccination and develop intestinal form of disease. This paper aims to present a protocol for diagnosis and specific treatment in intestinal form of CPV infection in puppies with non-protective titers of antibodies. Materials and Methods Diagnosis and specific treatment in intestinal form of CPV infection in puppies with non-protective titers of antibodies has been bone in one litter of Rottweiler puppies [one female (15 kg) and two males (17kg and 20kg)] 25 weeks old, with gastroenteric syndrome (vomiting and bloody diarrhea) and vaccinated against canine distemper (D), infectious hepatitis (H), parvovirosis (P), parainfluenza (Pi), leptospirosis (L) and rabies (R). Method used for CPV antigen detection were PARVO IC (Agrolabo S.p.A., Italy), and for CPV antibodies semi-quantitative titration were ImmunoComb Canine Parvo & Distemper IgG Antibody Test Kit (Biogal-Galed Labs., Israel). Three transfusion bags (250 ml) collected from mother and maternal aunt of puppies [with high level of CPV-Ab (>6 in Comb scale)] were used in specific therapy of CPV. Mother, maternal aunt and puppies were DEA1.1 negative (RapidVet-H, Agrolabo S.p.A., Italy).

Breed : Rottweiler Sex : one female and two males Weight : 15 kg 20 kg Age: 25 weeks Clinical status: gastroenteric syndrome - vomiting - bloody diarrhea Vaccinated: DP / DHPPi L / DHPPi LR


PARVO IC antigenic test (Agrolabo S.p.A., Italy)

The ImmunoComb Parvo and Distemper Test Kit is intended to evaluate the IgG antibody response to vaccination or infection by Canine Parvovirus (CPV) and Distemper Virus (CDV). A high antibody level is associated with immunity to infection (in well dogs) and is helpful for diagnosis of clinical cases. Low titer of CPV-Ab


Levels of IgG and IgM in Days Following Vaccination

Interpretation of Results

ImmunoComb Canine Parvo & Distemper IgG Antibody Test Kit (Biogal-Galed Labs., Israel)

High titer of CPV-Ab

Example of developed Comb

Mother and maternal aunt of Rottweiler puppies, vaccinated annually for DHPPi LR
Canine blood groups identification based on the agglutination methodology.

DEA 1.1 negative

Reagents and materials used in a canine blood groups identification based on the agglutination methodology (RapidVet-H, Agrolabo S.p.A., Italy)

Seven days

Results and Conclusion The following information from case history was significant: immunization with DP vaccine to 49 days and 63 old, DHPPi vaccine to 77 days old and DHPPi-LR vaccine to 91 days old. PARVO IC test (Agrolabo S.p.A., Italy) revealed CPV-Ag in stool of all puppies. ImmunoComb test revealed very low CPV-Ab (0-1 Comb scale) in blood of puppies. Each puppies received general treatment (antiemetics, fluids, antibiotics) and specific therapy (250 ml whole blood; complete transfusion within 90 minutes). Seven days of taking blood transfusion all the dogs were clinically recovered. These data suggest the usefulness of blood with high titer of CPV in Comb scale in therapy of intestinal form of CPV.
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