Updated: October 2013

Biotechnology Journal Instructions to Authors

Manuscript submission: http://mc.manuscriptcentral.com/btj Contact the Editorial Office at: btj@wiley.com

As of January 2014, Biotechnology Journal will be available as a purely online publication in response to environmental concerns and changes in user requirements. We will continue to publish the latest breakthrough research in biotechnology while reducing our environmental footprint. Authors will have the added benefit that there will no longer be color charges or any other charges associated with publishing in Biotechnology Journal (with the exception of those authors who opt-in to OnlineOpen, for which a fee is levied to make the article freely accessible to all). Authors are requested to follow these Instructions carefully. Manuscripts missing all necessary information requested for peer review will be returned to authors – this inevitably leads to a delay in the editorial processing of the manuscript. Please refer to our Appendix B for Authors for a quick checklist. 1. About Biotechnology Journal .......................................................................................................... 2 2. Manuscript preparation .................................................................................................................. 5 3. Online submission of manuscripts ................................................................................................ 12 4. Manuscript publication ................................................................................................................. 13 Appendix A: BTJ Keywords .................................................................................................................... 15 Appendix B: Checklist for Authors ......................................................................................................... 16

BTJ promotes a special emphasis on:         Systems Biotechnology High-Throughput and Microarray Technologies Synthetic Biology and Metabolic Engineering Nanobiotechnology Medical Biotechnology Translational Biotechnology and Applied Microbiology Methods and Advances Biosafety. the journal welcomes unsolicited submissions both for primary research articles and for reviews. A IMS AND SCOPE In particular. and biosafety or biosecurity. engineering materials for biological application. biochips. T-cell therapies. xenotransplantation. knockouts. 1. systems biology etc. medical biotechnology (including antisense/siRNAs.biotechnology-journal. targeting. imaging. re-design of natural biological systems for useful purposes.1. tissue engineering and biomaterials). transgenic animals. vaccines and applied immunology (Ab engineering. ethical and cultural aspects of biotechnological research in the Forum section. Biotechnology Journal is edited by Sang Yup Lee and Alois Jungbauer. data deconvolution). antigen delivery vectors and approaches. microand nanofluidics. therapies exploiting innate immunity. algorithms.). legal.com/btj Contact the Editorial Office at: btj@wiley. Computational analysis of all topics mentioned below (modeling.g.com Page 2 of 17 . enzymes and metabolites in a metabolic pathway). proteomics. Tcell therapies). plant/agricultural. illustrating the latest research results. all -omics fields (genomics. devices and systems. Special attention is also paid to the public. tissue engineering. enzymes. metabolomics. regenerative medicine (stem cells. reproductive cloning.). drug delivery therapeutics. DNA or RNA therapeutics (gene therapy.1. and biomimetics. bioinformatics (algorithms and modeling). xenotransplantation. In addition to special issues. etc. immunogenicity). analytical biotech (sensors/detectors for analytes/macromolecules). BTJ welcomes submissions on: Systems Biology/Bioinformatics: Overview and interplay between biological system components and their functional implications (e. Nanobiotech/ Biomaterials: Nanomaterials for industrial (white) biotech. Some issues are devoted to a special topic. Medicine/ Red Biotech/ Stem cells: Therapeutic cloning (somatic cell nuclear transfer). Synthetic Biology: The design and construction of new biological parts. Rapid Communications and Technical Reports. food or environmental biotechnology.manuscriptcentral. Biotech Ethics. A BOUT B IOTECHNOLOGY J OURNAL Biotechnology Journal (BTJ) is an international resource for both biotechnology researchers and professionals in related disciplines. translational immunology (antibody engineering.com Manuscript submission: http://mc. Mammalian Biotech/Genetics: Manipulating gene structure and control of gene expression. the Journal publishes strictly peer-reviewed papers covering novel aspects and methods in all areas of biotechnology. providing the latest comprehensive information on the most crucial areas of research and technological advances. and nanosystems for imaging. biopharmaceutical and enzyme The Journal contains original papers in the form of Research Articles. nanosensors. BTJ welcomes submission of papers in areas including DNA/protein engineering. molecular imprinting. Fully comprehensive in its scope. Science Communication www. peptide. viral or nonviral strategies for drug delivery.

and public authorities. mass spectrometry.com/btj Contact the Editorial Office at: btj@wiley. www. characterizations without functional data or applications. fungal or plant cells. fermentation optimizations without technical novelty. purification. arrays. Biosensors: (Meta)genomics. environmental scientists. microbiologists. electron microscopy/tomography. gene function analysis (e.biotechnology-journal. targeting and Metabolic Engineering: Genetic manipulation of species of interest to modify or allow the production of a commercially or therapeutically relevant compound. 1. novel organism/cell/molecular imaging techniques. but not limited to: biotechnologists. structural genomics. Food Biotechnology: Application of microbiology and metabolic aspects to food and beverage systems. for example cloning without functional relevance. and packaging after fermentation or bioconversion. bioengineers.2. separation. biopharmaceuticals. monitoring.com Manuscript submission: http://mc. Omics. metabolomics (chromatography. Manuscripts submitted to BTJ are first assessed by the Editors-in-Chief.production. forest biotech. food bioactive compounds. biosecurity and genecontainment strategies. chemogenomics. screening methods. food biotech. fluorescence microscopy. Analytical methods and systems for monitoring research. drugs.g. nutraceuticals.1. etc. In silico predictions with no supporting experimental data or implications for further studies will also not be considered. Methods: Technical aspects and practical protocols for all biotech applications. NMR. Analytical Biotech. disease. pharmacogenomics (SNPs). or separation studies without a modeling support. microbial biodegradation. enzymes. manuscripts not within the scope of the Journal will be sent back to authors within 10 working days without peer-review. Biotech Communication Ethics. gene structure analysis.1. arrays and chips. high-throughput imaging tools for drug screening. plant vaccines or bioreactors. food and nutrition scientists. Biocatalysis: Evolution and protein design (antibodies. Science 1. SAGE). use of enzymes as industrial catalysts.manuscriptcentral. proteomics (e. etc. O U T O F SC O P E Biotechnology Journal will NOT consider submissions consisting of marginal advancements.g. insect. food fermentation. SPR). patent lawyers. yeast or other organisms. environmental biotech: bioremediation. medical and pharmaceutical scientists. mass spectrometry). transgene expression strategies. expression systems in mammalian.) White/Industrial Biotechnology: Chemical synthesis by bacteria. yeast two hybrid. food safety and pathogens. biofuels. Plant Biotechnology: Crop improvement (resistance to stress. R EADERSHIP Including. pests). bacterial. bioenergy. Biosafety. phytoremediation. Biochemical Engineering/ Downstream Processing: Chemical engineering aspects of biological processes from primary material to product recovery. confocal imaging. biomining. agriculturists.com Page 3 of 17 . adsorption and chromatography studies without a deeper insight into the process. development and production of biotechnological products.

whereby it may be difficult to rephrase particular protocols. P E R MI S SI O N T O R EP R O DU C E FI GU R ES O R D AT A . will result in rejection/retraction of the manuscript.3. but not limited to.manuscriptcentral. data fabrication. www. The authors agree to this condition by submitting a manuscript to Biotechnology journal.biotechnology-journal. including.2.com/btj Contact the Editorial Office at: btj@wiley. All materials and reagents that are not commercially available (antibodies. S HA RI N G O F M AT ERI A L It is the authors’ responsibility to obtain permissions for reproduction of figures.3. etc. P L AGI ARI S M All manuscripts accepted for publication will be cross-checked with existing publications for plagiarism. All instances of publishing misconduct. or text from published works.org. In such cases. etc.1.) and associated protocols detailed in manuscripts published in Biotechnology Journal are to be freely available to academic researchers in a timely manner upon request. tables.com Manuscript submission: http://mc.uk. E T HI CA L MI S CO N D U CT It is the author’s responsibility to obtain and keep a copy of these permission statements This Journal endorses the COPE (Committee on Publication Ethics) guidelines and will pursue cases of suspected research and publication misconduct. authors are encouraged to refer to the original paper and only include modifications that were made in the current manuscript. constructs. Reference to any unpublished data of others also needs permission statements. 1.3. the Journal will follow the processes set out by COPE.1. the publisher of a journal or book is the copyright owner from whom the written permission must be obtained. plagiarism. CI T I N G O F UN P U BLI S H ED DA T A 1. For sections such as the Materials and methods. P UBLICATION E THICS 1. cell lines.publicationethics.4.3. Permission from authors is also encouraged as a professional courtesy.3. In many cases.com Page 4 of 17 . 1.3. For more information about COPE please visit the COPE website at http://www. image/data manipulation to falsify/ enhance results.

Technical Reports describe the development of a novel method or an improvement or noteworthy modification of an already existing technique or platform used in biotechnology.com/bauthor/en glish_language. These manuscripts should bear  www. Full-length reviews should not exceed 8000 words (including references and figure/table legends). iv. Rapid communications should not exceed 2500 words (including references as well as figure and table legends) and contain no more than three display elements (figures and tables). There are two types of reviews:  Reviews (full-length) should be reserved for subject areas that have not been previously reviewed in detail. Materials & methods. Technical Reports should not exceed 4000 words (including references as well as figure and table legends) and contain no more than three display elements (figures and tables). Acknowledgement and References.asp. Suggestions for language service providers can be found at http://authorservices. Reviews are to be invited by the Editor.com Manuscript submission: http://mc. American spelling is preferred. These manuscripts should bear the words “Technical Report” immediately above the title on the first page. ii. S CI EN T I FI C Four categories of scientific contributions are published in Biotechnology Journal: i. T YPES OF CONTRIBU TION S CO N T R I B UT I O N S 2.2.biotechnology-journal. Longer manuscripts will be considered but only if the increased length is a reflection of the amount of data presented and not a result of inclusion of unnecessary information. Acknowledgement and References. Research Articles should be divided into the following sections: Introduction. Discussion. Discussion. Conclusion. Results.2. Results & Discussion.2. 2. Authors planning to submit unsolicited reviews should send a brief outline of its contents and a short CV to the Editorial Office (btj@wiley. Technical Reports should be divided into the following sections: Introduction. M ANUSCRIPT PREPARATIO N ASSISTANCE the words “Rapid Communication” immediately above the title on the first page. The articles should be divided into sections that are appropriate to the topic. except in the form of a preliminary communication such as meeting abstracts.com Page 5 of 17 .com) prior to writing the full article. Mini- 2. Mini-Reviews provide an update of the latest research. Materials & methods. Original research articles should not exceed 6500 words. and authors less familiar with English usage are advised to seek the help of English-speaking colleagues.manuscriptcentral. Authors should refer to previous reviews for detailed background of the subject area. this limit includes references. Manuscripts may not have been published previously. Manuscripts must be grammatically and linguistically correct. Rapid Communications should be divided into the following sections: Introduction. figure legends and tables.1. Acknowledgement and References. Research Articles Rapid Communications Technical Reports Reviews Research Articles describe complete investigations. We recommend a maximum of five display elements (figures/tables). Rapid Communications describe the latest biotechnological discovery in a brief and succinct form.1. Materials and methods. L ANGUAGE Biotechnology Journal publishes articles in English.com/btj Contact the Editorial Office at: btj@wiley.wiley. Results. iii. These authors may also choose to have their manuscript professionally edited prior to submission.

com Page 6 of 17 . Conflict of Interest statement: authors are responsible for disclosing all financial and personal relationships between themselves and others that might bias their work. either completely.biotechnology-journal. The manuscript has not been submitted to another journal and will not be published elsewhere within one year after its publication in this journal. These articles are invited by the Editors. If no such rules or permissions have been iii.manuscriptcentral.reviews should not exceed 4000 words (including references and figure/table legends).    Industry highlights Ethical issues surrounding biotechnology Biotech around the world Please contact the Editorial Office (btj@wiley. O T HE R CO N T R I B UT I O N S Biotechnology journal also accepts Other contributions of the following types: Commentaries describe the latest research results published in the same issue of Biotechnology Journal.com Manuscript submission: http://mc. and also relevant ethical and socioeconomic issues. Examples include: .Important publications and awards .2. iv. Recommended word limit is 2000 words (including references and figure/table legends). Review articles are strongly encourage NOT to include previously unpublished data. of the laboratory and of the investigator) should be stated if animal experiments are included. Why the paper is suitable for publication in Biotechnology Journal? All authors concur with the submission and have seen a draft copy of the manuscript and agree with its publication. Intentional omission of any potential conflict of interest may however. vi. authors are also encouraged to submit summary figures that support the thesis of the Commentary. Meeting abstracts or preprints do not constitute prior publication.com/btj Contact the Editorial Office at: btj@wiley. 2. The permission of the national or local authorities (giving the permission or the accreditation no. C OVER LETTER The manuscript should be accompanied by a letter of transmittal that includes the following statements: i. negatively impact the decision to publish the manuscript. 2. www. As the peer-review process of Reviews do not allow for detailed scrutiny of research data. Authors are encouraged to avoid excessive reference to their own unpublished data. Publication in any reasonably retrievable source constitutes prior publication. Forum Articles describe the latest news and events in biotechnology. authors must state explicitly whether potential conflicts do or do not exist. Any new primary research data should only be in support of the topic of the review and NOT form the basis of the review. in part. reviews SHOULD NOT be a mere listing of available information.Institute or company highlights  New trends or policies  Meetings reports v.2. To prevent ambiguity. Authors are also encouraged to raise controversial opinions these however. This statement will not influence the decision.3. The manuscript does/does not contain experiments using animals. the article should provide novel insights and synthesis of available literature within the topic of the review. reviews should provide a balanced view of the field by citing and discussing relevant data. The work has not been published elsewhere.com) before writing or submitting a contribution to the BTJ Forum. ii. or in another form. must be supported by relevant literature and NOT the author's unpublished data. Commentaries discuss the studies in light of current knowledge. For both review types.

present the hypothesis. implicated in the particular country.com Page 7 of 17 . Therefore. The limit is 150 words. and full family name) of the authors and the name of their institute(s).containing only the most important keywords pertaining to the subject matter.4. trained reader may repeat the studies.2.1. Any materials and experiments that are used only in the Supporting Information. abbreviations should be restricted to a minimum. Abbreviations should be introduced Authors are requested to provide the “Practical Applications” of your work.com Manuscript submission: http://mc.4. Permission statements relating to points 4 and 5 should also be included in the text of the manuscript. Keywords: provide a list of 3-5 keywords v. Keeping in mind that abbreviations may be a hindrance to those not familiar with the field of study. A B ST RA C T An Abstract of the manuscript should be included. This should provide a brief background to the study rationale. It should not exceed 200 words. iv. P RA CT I C A L A P P LI C AT I O N S The first page of all manuscripts should contain only the following: i. Authors are www. T I T L E AND STYLE OF MANUSCRIPTS PAGE 2. the affiliations of all authors should be clearly stated by using superscript numbers after the name and before the institute. only when repeatedly used.vii. A list of abbreviations used in the paper. If the publication originates from several institutes.com/btj Contact the Editorial Office at: btj@wiley. and emphasize the takehome message of the study. This should also include the e-mail address. The manuscript does/does not contain human studies. it should be stated that local Ethical Committee approval was received for the studies and that the informed consent of all participating subjects was obtained. 2. Abbreviations used only in a table or a figure may be defined in the legend. This text should highlight the uses – either actual or potential – of the research described in the manuscript. Name. do not need to be described in the main text and instead should be included in the Supporting Information.manuscriptcentral. this must be stated.4. highlight significant findings/results.5. M AT ERI A LS AN D M ET H O DS The Materials and Methods section should describe all materials and methods used in the study in sufficient detail so that an independent. brief description of the methods used.4. This text should also be included on the second page of the manuscript. Permission statements relating to points vi) and vii) should also be included in the text of the manuscript. F ORMAT 2. The study rationale/hypothesis should also be clearly described. Full names (including full first name.3. Title of the paper . The abstract should not contain any references.4.4. This does not apply to Reviews and Other contributions.biotechnology-journal. 2. middle initial/s. I N T RO DU CT I O N The Introduction section should contain a description of the problem under investigation and a brief survey of the existing literature on the subject. This must be selfexplanatory and intelligible without reference to the main text.4. title and full postal address of the author to whom all correspondence (including galley proofs) is to be sent. ii. 2. 2. iii. If such studies are included. Providing this information will ensure the content is widely accessible by both academic and corporate audiences.

2. Either: The authors declare no commercial or financial conflict of interest Or: Conflict of interest: Nature of the conflict of interest. At the time of first submission a statement about any conflict of interest MUST be included in the cover letter and in the manuscript after the Acknowledgements section.g. they should also be typed with double spacing throughout. Titles of journals should be abbreviated according to the practice of PubMed.com/btj Contact the Editorial Office at: btj@wiley. should be numbered sequentially in the order in which they appear in the text. R E S UL T S The Results section describes the main experimental finding of the study.6. The name of each test used and its outcome should be detailed. References.7.com Page 8 of 17 .com Manuscript submission: http://mc. 2.10. If necessary. the Introduction and the Results sections. in which the Results and Discussion sections are combined.manuscriptcentral. if no print reference is 2.encouraged to refer to previously published descriptions of methods and provide only a brief summary of the method.8. References are to be collected in numerical order at the end of the manuscript under the heading “References”.11. 2. including those in tables and figure legends.4. e. A CK N O W L E DG E M EN T S Acknowledgements should be provided on a separate page at the end of the text (before the References).4. Full statistical analysis should be performed.biotechnology-journal. may negatively impact the decision to publish the manuscript. cite unpublished or personal work in the text but do not include it in the reference list. The numbers should be set in square brackets in the text i.4.9. Any modifications to published methods should be described in detail. Intentional omission of any potential conflict of interest however. 2. "Author X is a paid consultant for company Y. C O N C LU DI N G R E M AR K S 2.4. to a biotech or pharmaceutical company).O F . It should enrich. The abbreviated journal title and the volume number should be in italics. The DOI for the reference should be included at the end of the reference. R E F E RE N C E S The Concluding Remarks section is only applicable to Rapid Communications and Technical Reports. 18]. Articles that have been submitted but not yet accepted should NOT be included in the reference list." This statement will not influence the peer review and the decision on the manuscript. authors must state explicitly whether potential conflicts do or do not exist. Concluding Remarks should provide the take-home message in a short and succinct manner. [2. or external contractor. To prevent ambiguity.I N T ER E S T ST AT E ME N T Authors are responsible for disclosing all financial and personal relationships between www. ALL funding sources for the study and each author’s contribution to the article should be stipulated. Sources (name and location) of reagents and equipment should be provided. but not repeat.4.e. themselves and others that might bias their work (e. D I S C US SI O N The Discussion section describes the findings in light of current knowledge. an author writing on advances in basic research on antibiotics might be a paid consultant. as should the number of samples. replicates and data presentation (SD vs SEM). C O N F LI C T . Subsections should be provided to reflect the key messages of the study.4.g.

... Biotechnol.4. Fairbrother J. 1996. H. Please note that website addresses must not be included as a reference but should be inserted in the text directly after the data to which they refer. Eng. John Wiley & Sons. [6] Nadeau. E.. Supporting Information should be cited within the article text.Williams. Direct conversion of methane to hythane. 3D structures..14. The figures should be submitted separately and not included into the main document. Ahmed. US Patent Application 2007/0218035 A1.com/btj Contact the Editorial Office at: btj@wiley.K. b). and typed at the bottom of the Table. in: Heller...1002/bit. c). Genetically Engineered Food – Methods and Detection. figures. 557–562....com Page 9 of 17 . 2004. J. Perspectives of genetic engineering of bacteria used in food fermentations.biotechnology-journal. Bone repair with a form of BMP-2 engineered for incorporation into fibrin cell ingrowth matrices. Column headings should be kept as brief as possible and indicate units.available. for these reasons. P. J. C.). Footnotes to tables should be indicated with a).4..4. 100– 118. R.manuscriptcentral. J. C. Patents: [5] Pandey. and a proof is not made available prior to publication. K. (Ed. J. Bioeng.. F. audio clips. and a descriptive legend should be included. Examples of Supporting Information include additional tables. (Ed. Klee. Use of live bacteria for growth promotion in animals. and other related nonessential multimedia files.. authors should provide any Supporting Information in the   www. Ratnani. J. It is published as supplied by the author. S UP P O RT I N G I N FO R M AT I O N Supporting Information can be a useful way for an author to include important but ancillary information with the online version of an article. Life Sci.12.. Note that  Papers with multiple authors should be limited to listing the first four authors. K. Eck. etc. The legends should not appear under the figures. Other serial publications should be cited in the same manner as journals. Examples: Journals: [1] Liebeton. pp. 4. Papers published online in advance of print should be cited with their DOI. Identification and Expression in E. 2. A. Handbook of Plant Biotechnology. S. E. Gratz. [2] Schmoekel. T A B L ES Tables with suitable captions at the top and numbered with Arabic numerals should be collected at the end of the text on separate pages for different tables. Each Figure must have a separate legend. US Patent 5516967.com Manuscript submission: http://mc. followed by et al.13.20168.Weber. 2005. 2. [4] Chrisou.  References in tables and figure legends should be listed AFTER the references in the main text 2. F I GU R ES AN D L E G EN DS Figures should be numbered in the order of their appearance in the text with Arabic numerals.W. Abstracts and posters in meeting books must not be cited unless they are generally accessible. data sets. et al. Schense. Books: [3] Geis. 2004. DOI: 10. Wiley-VCH Verlag. but be gathered in a separate section (Figure legends) after the references and tables. K.). movie files. Weinheim 2003. coli of novel nitrile hydratases from the metagenome. which should be self-explanatory and allow readers to understand the data presented without reference to the main text.

Where possible. no enlargement or reduction should be necessary. Symbols and labels should be a height of 1. and 0. or scale. An image viewed at 100% on screen may look fine but will not necessarily reproduce well as the screen resolution is much lower (only 72-96 dpi) than that of a printing press.com/btj Contact the Editorial Office at: btj@wiley. C. resolution: 300 dpi). then the image will not reproduce well in print.  Make sure that any labeling is legible against the background. it is not necessary to submit graphics with high resolution. however. high resolution graphics may be requested.4 pt for    Do not use higher resolutions than those given in the Table above as these will not improve the quality of your image but will produce very large files. Make the image orientation the same as that intended for publication. If this is not possible. B. a width of 1800 pixels would be required to obtain a resolution of 300 dpi. Flatten images prior to submission. it is essential that the resolution is sufficient so that the reviewer is able to accurately assess the work.wiley.com Manuscript submission: http://mc. The recommended minimum line weight is 0. figures to the size intended for publication. etc. Images (photos) with lettering should be saved at a higher resolution (minimum 600 dpi) than a photographic image alone www. all comparable labels should have the same size lettering. If the size is increased to six inches.       2.desired final format.5. i.0 mm at their final published size.e. a photographic image that is three inches (76.com/bauthor/su ppinfo. Type. however.com Page 10 of 17 . a width of 55–85mm or 120–175mm.2 mm) across at final size should be saved as TIFF with a width of 900 pixels (final size: 3 in.manuscriptcentral. For further information on recommended file types and requirements for submission. G UIDELINES FOR FIGURE PREPARATION white lines on a black background. and that lines are of a suitable thickness. they should not contain layers and/or transparent objects Figures should have the following resolution at their final published size: ´ Graphs Photos Color (RGB) 800-1200 DPI 400-800 DPI 300-400 DPI For initial submission. Figures should be submitted in a format which can be reduced to publication size i. Remove excess space and elements from around the image.  Figure panels should be indicated by bold upper case letters (A. An easy way to check the quality (resolution) of figure files is to use the zoom function: if an image viewed at 400% on screen is blurry (pixelated).biotechnology-journal. Changing the size of a digital image changes the resolution. lines or other elements not intended for publication should be removed before submission. authors are encouraged to submit Supporting Information as a single PDF file. do not define lines as ‘hairline’ width. as an example. as the area is increased the overall dpi actually remains the same. Upon acceptance.5–2.) on the top left hand corner  As far as possible. Gridlines are not allowed except for log plots.3 pt for black lines on a light background.e. and the font size should be consistent throughout the Figures. Use standard fonts such as Arial and Helvetica.asp It is at the Editor-in-Chiefs’ discretion to decide which part of the manuscript will be published as Supporting Information. Please prepare your figures according to the following guidelines:  Crop. Each figure should be uploaded in a separate file. please visit: http://authorservices.

however. if at all possible. including the background. 2. experiments. I MA G E Manipulation of images is unacceptable. especially when the image contains large areas of single color or repeating textures and patterns. preferably in the Figure legends to allow each Figure to be easily understood without reference to the main text of the article. and should note that analyzing replicates within a single experiment does not provide information regarding experimental reproducibility. E Q UAT I O N S Mathematical and chemical equations are to be written in the manuscript at the place in which they belong and should be marked by Arabic numerals in parentheses in the right margin in the order of their appearance. added. Remember that supplying high-quality electronic artwork can reduce delays in production time as it minimizes the need for artwork to be resupplied.com/btj Contact the Editorial Office at: btj@wiley. The name of each statistical test used and the results obtained (including a description of the comparisons made to obtain the p values. or re-positioning of lanes/fields. Try to avoid adding more to your graph or illustration than is necessary.   (minimum 400 dpi) to avoid the text and line art appearing jagged. obscured or moved. O THER GUIDELINES 2. S T AT I ST I C AL AN A LY SI S – ST AN D A RD R EQ UI R EM E N T S 2. In cases where manipulation is unavoidable. removal of background noise/bands. Unacceptable manipulation includes.biotechnology-journal. but is not limited to. They must be numbered in consecutive order with the other figures. lines and symbols (if there are several symbols.5.1.1. eliminated. the enhancement of one feature/ band over others. vital data are not removed. 2. MAN I P U L AT I O N full blots or gels that the figures are derived from are supplied in the supporting information.6.com Manuscript submission: http://mc.com Page 11 of 17 .manuscriptcentral. Cropping of gels. ensuring that all original data.6. if not clearly indicated in the Figures themselves) should be stated. software and processes used to obtain the images must be fully detailed in the manuscript either in the Figure legends or in the Materials and methods section. this should be clearly detailed in the Figure legend. The number of samples.6. an explanation of the alterations is included in the Figure legend and images of Full statistical analysis of your data should be detailed in the manuscript. Information should not be enhanced. S T RU CT U RA L FO R M U LA E Structural formulae should be drawn in the manuscript in the position where they belong.3. most software has an option to compress the file using LZW compression and this will produce smaller files. etc. stipples. excessive shading. Photographic images often produce very large files. be wary of using shades too close together – an ideal separation is 20%. All instruments. is permitted providing that all alterations are clearly indicated by the use of dividing lines in the image itself. Authors must be able to produce all data in their raw format upon editorial request. When using shades of grey or other tints. try and add them to the legend rather than a key).) should be noted. www. replicates etc. All figures must accurately reflect the original data. remains visible and that no new features are introduced. Authors are encouraged to analyze data across multiple experiments.2. 2. Avoid 3D charts. Keeping an image simple is the best way to produce good output. should be detailed. data presentation (mean +/– SD or SEM etc.6. Acceptable image manipulation includes uniformly adjusting the contrast of an entire image and any control images.

online help is available. In the first round of     www.1.3 Cover letter. Prepare cover letter as per the guidelines in section 2.com/btj Contact the Editorial Office at: btj@wiley.3. Follow the step-by-step instructions in the system to supply the requested information .manuscriptcentral. O RIGINAL Biotechnology Journal offers a web-based manuscript submission and peer review system accessible at: http://mc.manuscriptcentral.biotechnology-journal.com Page 12 of 17 . Create an account in the system by clicking on the “Create an Account” button. it is not necessary to upload files separately – authors may choose to upload all relevant information in ONE WORD or PDF file. Keywords: entering appropriate keywords (see 3.com/btj To submit manuscripts online:  Prepare manuscript and illustrations in the appropriate format.com Manuscript submission: http://mc. O NLINE SUBMISSION OF MANUSC RIPTS MANUSCRIPTS manuscript peer-review. according to the instructions given (see Section Format and style of manuscripts) and ensure that data are given in the correct order and style for the journal.

      Appendix A: BTJ K EYWORDS ) speeds up the peer-review process by facilitating the selection of appropriate reviewers. the corresponding author receives an e-mail with a unique code and link.2. To monitor the progress of the peerreview process. Check the final PDF! The information in this final PDF preview is the same as that seen by the editors and reviewers. please contact the Editorial Office at btj@wiley. P ROOFS AND REPRIN TS . There may be a short delay from when the article is accepted by the journal and when it has been received at Wiley-Blackwell. Upload the two documents above to the Online Submission site. A UTHOR S ERVICES Once an article is accepted. R EVISED MANUSCRIPTS Authors are given a suitable period to revise their manuscripts – should this not be sufficient. Alternatively. Logging in to Author Services with the same e-mail address where the alert is received automatically connects the author to the article. Any questions concerning the online submission process should be directed to the Editorial Office at btj@wiley. Authors may exit/re-enter at any stage during the preparation stage.com for a prolongation. All submissions will be converted to PDF format during the upload process. authors may register with any e-mail address: 4.     4. Go to http://authorservices. only the changed files need to be replaced. Reviewers: authors are requested to suggest at least four suitable reviewers and may also exclude potential reviewers by selecting them as “non-preferred” reviewers – a reason for this should be included in the cover letter. When an article is accepted. Prepare a revised manuscript that contains the changes highlighted by either changing the font color or using the “track changes” mode.com/bauthor/ for further information. Once a manuscript is submitted. Wiley’s Author Services provides the functionality for authors to track the production progress of their articles. it is therefore critical that all information are provided in this file prior to clicking the “submit” button.2. 3.com. it is therefore critical that all information are provided in this file prior to clicking the “submit” button.1. Prepare revision as follows:  Prepare a point-by-point response letter – reviewers’ original comments should be followed authors’ response to the comment. Please note that when revised manuscripts are submitted online. authors may login periodically to the Author Center for status updates. M ANUSCRIPT PUBLICATIO N use the "add article" feature and enter the unique code contained in the initial e-mail to connect to the article. Authors will also receive free access to the final published version of their articles. Check the final PDF! The information in this final PDF preview is the same as that seen by the editors and reviewers. The system automatically generates one PDF file which contains all parts of the manuscript. 4.wiley. no changes are allowed until an editorial decision has been made.

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A PPENDIX A: BTJ K EYWORDS BTJ primary keywords/areas of expertise: select at least 1 Analytical biotechnology Biochemical engineering Bioprocess engineering Food biotechnology Green/plant biotechnology Nanobiotechnology Red/medical biotechnology Systems biology White/industrial biotechnology Biocatalysis Biomaterials Downstream processing Computational biology Metabolic engineering Omics Science communication Synthetic biology Other BTJ secondary keywords/areas of expertise: select 3-4 Algae Bioelectronics Biofuels Biophysics Bioremediation Biosynthesis Cell biology Chromatography Drug delivery Fermentation Gene regulatory network Genomics Insect cells Medical applications Micro/nanofluidics Modeling Polymers Protein expression Proteomics Scale-up/down Textile biotechnology Translational medicine Antibodies Bioenergy Bioinformatics Biopolymers Biosafety Biotransformation Cell culture Crystallization Environmental biotechnology Food Gene therapy Glycobiotechnology Mammalian cells Metabolic flux analysis Microarrays Molecular biology Process development Protein folding Recombinant proteins Screening Tissue engineering Vaccines Bacteria Bioengineering Biomaterials Bioreactor Biosensors Blue/marine biotechnology Cellular engineering Diagnostics Enzymes Fungi Genetic engineering High-throughput Mass spectrometry Metabolic network Microbiology Molecular engineering Process monitoring Protein purification Regenerative medicine Signaling network Transcriptomics Viruses Biochemistry Bioethics Biomedical engineering Biorefinery Bioseparation Cancer Cellular therapy Diseases Expression systems Gene expression Genetics Imaging Materials Metabolomics Microreactors Plants Protein engineering Protein stability RNA interference Stem cells Transgenic organisms Yeast .

Abbreviations: alphabetical order. EPS or PPT) at high resolution (300 dpi)     Please note that a fee has to be charged for color figure printing Supporting information online Extensive tables Text exceeding the maximum word count Other files such as animations and video sequences Figures and tables have to be accompanied by appropriate legends. Does the manuscript contain experiments using animals? 5.A PPENDIX B: C HECKLIST FOR A UTHORS  CHECKLIST Which manuscript type? 1. E. J. Why is the paper suitable for publication in BTJ? 2.1002/bit. Biotechnol. Full author names: (including first name) and the name of their institute(s). Weinheim 2003.. P. (Ed. timely and/or of such importance that rapid publication is warranted. 4. 2004. 3. [4] Chrisou.). et al. 3 display elements (Figures and/or Tables) 5. Technical Report: Short description of the development of a novel method or an improvement or noteworthy modification of an already existing technique or platform used in biotechnology. J.. G. Has to be submitted along with the manuscript body and in final form. K. Not more than 5 figures.. 5. including those in tables and figure legends. Identification and Expression in E. Jason C. using superscript numbers after name to indicate multiple affiliations. Do all the authors concur with the submission? 3.20168.. full postal address and e-mail address of the author to whom all correspondence (including galley proofs) is to be sent. Handbook of Plant Biotechnology. DOI:10. pp. Weber. Correspondence: name.. 2. Schmoekel. Bioeng. only standard abbreviations). Note that  When more than 5 authors: list 4. F. meeting report. Full-length Review: 8000 words maximum Mini-Review: 4000 words maximum 3.  Papers in press: cite DOI.. The first page of the manuscript should contain: 1. A. 18] in the order in which they appear in the text. Klee. should be numbered sequentially in square brackets [2. Other contribution: Forum column (500 to 2000 words). Wiley-VCH Verlag. 4. figure legends and tables. Figures and tables OK? Tables with title and footnotes included at the end of the text Figure legends at the end of the text Figures should be submitted in original format (preferably TIFF. book review Manuscript style and format OK? Manuscripts should be submitted in English (American spelling). 3 display elements (Figures and/or Tables) 4. Keywords: (3 to 5). Maximum 2500 words. coli of novel nitrile hydratases from the metagenome. title. [2] Hugo. et al.  Other serial publications: cite in the same style as journals  Website addresses not as references but inserted in the text directly after the data to which they refer  Abstracts and posters in meeting books must not be cited unless they are generally accessible     Personal communication or unpublished data should be cited in the text. (Ed.. 2004.J. in press. 2. K. Can you confirm that the work has not been submitted to another journal and will not be published elsewhere within one year after its publication in this journal? 4. in: Heller. (no typesetting or galley proofs before publication) Cover letter complete? Please state: 1. Bone repair with a form of BMP-2 engineered for incorporation into fibrin cell ingrowth matrices. 557–562. 2005. letter. Perspectives of genetic engineering of bacteria used in food fermentations. Reference list (collected at the end of the manuscript) as in the following examples: Journals: [1] Liebeton. Research Article: Describing complete investigations.. Books: [3] Geis.. Maximum of 6500 words. Eck. C. Rapid Communication: Brief. Maximum 4000 words. Eng. John Wiley & Sons. preferably as PDF. including references. 100–118. Life Sci. Review: Only invited by the Editors. The second page: abstract (maximum 200 words) Number the sections: 1 Introduction 2 Materials and methods 3 Results (or Results and discussion) 4 Discussion (or Concluding remarks) 5 References Reference format OK? References. Does the manuscript contain human studies? Suggest reviewers Provide the names and e-mail of four suitable reviewers . Genetically Engineered Food – Methods and Detection.). Title: (concise. H.