In this issue

Malaria News Guest Column Young Scientist Column Institute’s Activities Annual Day Celebrated Symposia/Workshops/Trainings Organized Scientific and Research Advisory Committee Meetings Organized Awards Received Research Papers Published (July–December 2008) Progress of NIMR (2004–08) 2 3 4 5 5 5 6 6 7 8

Fr om th e Dir ec tor’s De sk Fro the Direc ect Desk
Dear Readers, With great gratification I present this issue of Plasmodium. In the past six months, NIMR has achieved new heights in both quality and quantity of scientific accomplishment. During this period, NIMR has been identified as ‘WHO collaborating centre for laboratory testing and evaluation of public health pesticides’ and the ‘National referral centre for diagnosis of malaria’ by the National Vector Borne Disease Control Programme. The quality and quantity of scientific publications has been enhanced. Shifting to the new campus at Dwarka has been initiated and many activities have become functional in the new campus. NIMR is all set to start an integrated M.Sc.-Ph.D. course in Medical Entomology in affiliation with the Goa University, Goa. If I look back, NIMR has travelled far in almost every aspect during the last five years. Many pending assignments have been successfully completed and several new initiatives have been taken. We have gathered some of these activities and highlighted in this issue of Plasmodium. I wish you all a happy and prosperous new year. AP Dash

Editorial Board
Editor-in-Chief AP Dash Editors OP Singh, Aparup Das Anup Anvikar, Neelima Mishra Assistant Editor U Sreehari Production Jitender Kumar DS Sontiyal

National Institute of Malaria Research
(Indian Council of Medical Research), Sector 8, Dwarka, New Delhi–110 077 Telephone: +91-11-25365774, 25365904; Fax: +91-11-25365904 E-mail: director@mrcindia.org; Website: www.mrcindia.org

After plasmodia infect a blood cell. and in this study. According to the researchers. Source: ScienceDaily. However. c o m . Higgins found that a variable region of PfEMP1 covers a section that is important for docking up with the placental wall.C. just enough to allow anchoring to take place. These tests are storable for long periods of time at ambient temperatures and do not involve wet reagents. s c i e n c e d a i l y. as plasmodia contain many different varieties. s c i e n c e d a i l y. The team then developed an antibody to find a protein similar to SM1 that existed naturally in the parasite.com) immune response for PfEMP1. The diagnostic tests in the DxBox system run much faster than conventional tests in part because the liquids involved behave differently. saglin expression. New Target for Malaria Drugs Identified A new study has identified one of the tricks malaria uses to hide from immune proteins–a finding that may help in future drug development. low birth-weight. According to a report in Discovery News. still plagues humanity. The UW prototype cards look for the presence of malarial proteins. but the team is also working on other kinds of protein tests as well as a second kind of test for each disease that looks for the pathogen’s DNA or RNA. Essential Proteins for Critical Stage of Malaria Discovered Researchers at the Johns Hopkins Malaria Research Institute have identified the molecular components that enable the malaria-causing parasite Plasmodium to infect the salivary glands of the Anopheles mosquito—a critical stage for spreading malaria to humans. to detail how plasmodia protect these conserved areas. saglin. Germany. a structural molecule on blood vessels. a key factor for clinicians who have limited time to spend with their patients.Malaria News ‘Astronaut Food Approach’ to Medical Testing: Dehydrated. Nerlich and colleagues discovered that most people buried at the site died between the ages of 20 and 30. such as DNA amplification and gene sequencing. In this test. This tactic can be especially problematic during pregnancy as malariainfected RBCs congregate in the vessel-rich placenta (the source of food and oxygen for the growing fetus). certain parts of the protein have to remain constant for proper function.htm Earliest Evidence of Malaria Found in Ancient DNA of Egyptian Mummies The ancient DNA of two Egyptian mummies who died more than 3./ r e l e a s e s / 2 0 0 9 / 0 1 / 090116073203./ r e l e a s e s / 2 0 0 9 / 0 1 / 090121123049. then move into red blood cells (RBCs) to replicate and wait for the next mosquito to help continue the cycle. fever and more. The malaria-test card is being developed as part of an automated diagnostic system informally called the DxBox. Nerlich and colleagues believe that their work in identifying one of the earliest forms of the disease may help develop new treatments. malaria antibodies are dried in sugar matrices and were shown to retain 80–96% of their activity even after 60 days of storage at elevated temperatures. Retrieved 28 January 2009 from h t t p : / / w w w. The two proteins bind together to allow invasion of the salivary gland by Plasmodium sporozoites. Paul Yager. which greatly diminished salivary gland invasion in the mosquito. which can be transmitted to a human when bitten by an infected mosquito. Source: ScienceDaily. creating health problems such as anemia. Retrieved 28 January 2009 from h t t p : / / w w w. and colleagues described the prototype cards in the December issue of the journal Lab on a Chip. a mosquito salivary protein. Tests with the prototype malaria card can reach a result in less than nine minutes using an immunoassay. the team conducted experiments 2 Plasmodium . Matthew Higgins generated high-resolution 3-D structures of a malarial sticky protein that binds to placenta. c o m . which they use to evade the human immune system. enabling them to attach to blood vessels and escape destruction by the host’s spleen while they replicate. the variable region moves aside and ever so briefly exposes the binding region. Using special techniques from molecular biology. Marcelo Jacobs-Lorena and his colleagues found that saglin bound with the artificial peptide SM1. Through a series of experiments. In a previous study. To carry out the test. even months later. The DxBox consists of a portable. which they identified as TRAP. Wallet-sized Malaria Tests Promise Better Diagnoses in Developing World Researchers at the University of Washington have developed a prototype malaria test printed on a disposable Mylar card that could easily slip into your wallet and still work when you take it out. fully automatic reader being developed by Micronics that will process the card-based disposable tests. approach. The ancient scourge. To further prove the interaction between saglin and TRAP. they send out clusters of sticky proteins to the cell surface. or switch off. When the infected RBC gets close to chondroitin sulphate. which has shaped history by decimating invading armies and making villages in the grip of the fever hard to colonize. is a receptor for the Plasmodium protein Thrombospondin-Related Anonymous Protein (TRAP). Once inside their human hosts the parasites first set up shop in liver cells. PfEMP1. the Dx being medical shorthand for diagnosis.500 years ago has provided clear evidence for the earliest known cases of malaria. Pathologist Andreas Nerlich and colleagues at the Academic Teaching Hospital Munchen-Bogenhausen in Munich. UW bioengineering professor. But targeting these sticky proteins with drugs is difficult. or antibody-based. a clinician has to spot a drop of a patient’s blood onto a card and feed it into an instrument that gives a yes or no answer for a panel of infectious diseases in 20 min or less. studied 91 bone tissue samples from ancient Egyptian mummies and skeletons dating from 3500 to 500 B.htm to down-regulate. the researchers identified ancient DNA for the malaria parasite Plasmodium falciparum in tissues from two mummies. Higgins noted that women in regions where malaria is endemic do gain some immunity to the build-up of RBCs at the placenta after multiple pregnancies by developing an (Source : medindia.

falciparum. in late 2004. Plasmodium vivax is the most common species of malaria parasite outside Africa. the project did not start until the P. primarily due to a concentration of resources and scientific effort on the greater threat to human lives. on p.K. was featured as the cover article in the journal Nature. however. the material was shipped to TIGR where genomic libraries were constructed and highthroughput sequencing was undertaken. Dr Carlton has a keen interest in training scientists to use genomic data. Dr Carlton is passionate about genomics and the power that genomics technology has to revolutionize medicine. The Economist and USA Today. the Burroughs Wellcome Fund stepped up to the challenge and provided the additional funds for more monkey infections. she provides training and support for Indian students through a joint NYU Langone Medical Center-National Institute of Malaria Research grant with Drs Hema Joshi and A. almost six years to the day that the P. falciparum. vivax genome told us? Briefly. The P. vivax was completed. the most common malaria parasite in Asia and Latin America. which has the potential to invigorate the field of basic vivax malaria research. the National Institutes of Health and The Institute for Genomic Research. we now know that the genome has very G+C-rich internal chromosome cores that contain genes involved in housekeeping activities. vivax poses a less severe threat to human health. Maryland. an initial problem was obtaining sufficient DNA in order to be able to generate genomic DNA libraries for highthroughput sequencing. is the recipient of several multi-million dollar federal grants. At last. vivax in vitro culture has left the study of the biology and genetics of the species far behind that of P. Since P. What has analysis of the P. annotation. Dash. vivax project used funds from the U. In particular. An anxious period ensued during which many members of the vivax malaria community wrote supporting letters for several proposals to funding agencies in order to secure additional funds to complete the project. Recently. perhaps reflecting the perception that P. However. whereas the peripheral. With sufficient DNA generated from the successful infection of eight Saimiri saimiri monkeys. the impracticality of continuous P. Currently.GuestColumn The Plasmodium vivax Genome Project Jane M. New York. coordinated among three genome sequencing centers in the United States (U. the first genome sequence of P. her work deciphering the genetic code of P. and her work has been profiled by many media organizations including CNN. U. a groundswell of support among malaria researchers led to an international project to sequence the genome of P.S.S. Her own research involves decoding the DNA of important human pathogens. U.P. as well as parasites prevalent in the U. vivax cannot be grown in vitro and is present at low levels of parasitaemia in natural infections. Department of Defense and the National Institute of Allergy and Infectious Disease/National Institute of Health (NIAID/NIH) remaining from the first Plasmodium sequencing project. The project to decode the P.A. subtelomeric regions of chromosomes are highly A+T-rich and contain multi-gene families — in particular ~350 copies of the largest gene family contd.S. Reuters. funds to close the gaps in the sequence and to annotate and analyze the genome were exhausted. Georgia. with consequent losses in the development of novel methods of control. vivax genome was fraught with problems from the outset. Finally. BBC. including the University of Florida. falciparum sequence was close to completion. falciparum genome was published. that of severe malaria caused by P. Atlanta.S.) and the United Kingdom (U. and causes up to 40% of malaria cases per year world-wide. the paper describing the P. The project. vivax. such as the common STD Trichomonas vaginalis. and has taught at several World Health Organization/World Bank workshops in Africa. In the mid-1990s. thereby paving the way for NIAID/NIH to provide funding for closure. Despite this. with frequent exchange of scientists between New Delhi and New York. falciparum. The solution was to use a laboratory line from El Salvador (Salvador I) adapted to grow in Aotus and Saimiri monkeys in the 1970s and maintained at the Centers for Disease Control. Most recently. and analysis of the genome. 4 Dr Jane M Carlton is Associate Professor of Parasitology at New York University Langone Medical Center.). in particular species of malaria. and has spent the past 14 years as a member of several scientific institutions in the United States. The Salvador I line is designated a ‘reference strain’ because it has been passaged in human volunteers and through mosquitoes. She received her doctorate in Genetics at the University of Edinburgh in 1995. and was sequenced in its entirety at The Institute for Genomic Research (TIGR) in Rockville. vivax malaria is often referred to as ‘neglected’.S. and was finally published in October 2002 [1]. Carlton Associate Professor Department of Medical Parasitology New York University Langone Medical Center. NY 10010. vivax sequence appeared as the front cover article in Nature [2]. She has published more than 70 articles and book chapters. used resources from several funding agencies as well as expertise from many malaria researchers. and has been used for previous whole genome expression studies. Thailand and Brazil. Although there was some support for sequencing the genome of Plasmodium vivax during this time. January 2009 3 . after completion of genomic sequencing and construction of the sequence data into an ‘assembly’. and the project came to a halt in late 2003.

Nature 2002. some immune gene families such as LRIM1 and TEP1 were also found that limit the parasite load in malaria vector (Fig 1) [7]. 8. Science 2002. melanization also requires LRIM1 activity (horizontal arrow) [7] The Plasmodium vivax Genome Project contd. our comparison will help us predict what mutations might be involved. Carlton JM et al. In depth understanding of diverse immune gene families [8] provides insight as to how the parasites invade the malaria vector and proliferate without any interruption. 206: 3835–43. Trends Parasitol 2008. 316: 1738–43. Perhaps more striking is what the genome sequence did not tell us. 97: 6619–24. Unfortunately. we desperately need a continuous in vitro culture system so that researchers world-wide can have access to P. We know that several gene families have expanded massively in P. Proc Natl Acad Sci USA 2000. falciparum proteins known to be involved in drug resistance with their P. We also know that many of the genes are remarkably conserved between P. et al. 455: 757–63. falciparum and P. Osta MA. such as those involved in red blood cell invasion and immune evasion. the genome sequence has been used to elucidate the pattern of expression of all genes during the asexual blood stages [3].com/nature/focus/malaria/. vivax laboratory isolates adapted to growth in monkeys. 2.A. J Exp Biol 2003. vivax. a characteristic feature of infection with P. Moreover. Despite the destruction of parasite. 7. Christophides GK. 3. Hemlata Srivastava. Delhi Fig. The Plasmodium vivax genome article and associated papers can be viewed for free at http://www. Since one genome sequence is not enough for such intra-species studies. In contrast. a total of 338 genes were found from 31 gene families in An. should resistance arise in P. vivax biological material for their experiments. 2008. However. so that genetic markers of resistance can be identified and used to monitor its spread. et al. et al. References 1.S. 419: 498–511. Satisfyingly. 24: 545–50. Science 2002. Gardner MJ et al. et al. BMC Genomics 2008. gambiae [6] yielded fruitful clues of direct involvement of specific immune genes in regulation of Plasmodium development in vectors. several thousand genes remain classified as ‘hypothetical’ with no known equivalent in any other organism sequenced to date. we are now determining the sequence of six more P. CTLMA2 protect the remaining ookinetes from the melanization response (slanted black bars). 207: 2551–63. 6. how it will evolve in response to new drug regimes and potential vaccines.1: Schematic model of LRIM1 and CTL (CTL4 and CTLMA2) protein action during Plasmodium development in the mosquito midgut. but there is much that remains to be done. Many such immune gene families were identified in Anopheles gambiae (an African malaria vector) which is the only vector having entire genome sequence information [2]. and thus truly take advantage of the genome sequence. vivax parasite has undoubtedly propelled our understanding of human Plasmodium biology. et al. and other whole genome transcriptional studies are ongoing. gambiae [3. we need to understand how the parasite is evolving and in particular. et al. 5. In many recent studies. Interestingly. 298: 159–165. 4 Plasmodium . Int J Parasitol 2003. 4. References 1. J Exp Biol 2004. 2. Dimopoulos G. And finally. CTL4 and. Understanding the detailed mechanisms could be helpful in designing transmission blocking vaccines and helping in controlling malaria. functional characterization of immune genes in An. Shin SW. Nature 2008. vivax counterparts. we have been able to compare the structure of P. Cohuet A. vivax. Carlton JM et al. three out of four invading ookinetes are eliminated. 105: 16290–5. it did not tell us the precise identity of genes involved in relapse.YoungScientistColumn Genetic Basis of Innate Immune System in Malaria Vectors It is a well known fact that immune system genes are responsible in development of immunity in organisms against foreign bodies. These genes also play very crucial roles in innate immune system of malaria vectors against the invasion of malaria parasite [1].nature. the vir family. et al. vivax. vivax and that those that appear to be evolving quickly are likely to be involved in interactions with the host’s immune system. partly through the antagonistic action of LRIM1 (upward oriented arrows). 33: 933–43. from a non-genomics point of view. During or soon after invasion of the midgut epithelium (four downward-oriented arrows). however. 4. vivax.4] that are involved in cellular and molecular interaction at different levels of malaria parasite’s life cycle in vectors [5]. it was also found that many immune gene families facilitate transmission of parasite in malaria vectors such as C-type lectins that act as agonists and protecting parasite from innate immune response. Science 2007. We also need to expand studies into uncovering the molecular mechanisms of drug resistance in P. Decoding the genome of the first P. Bozdech Z et al. SRF Evolutionary Genomics and Bioinformatics Laboratory NIMR. see [4]). Finally. et al. to a lesser extent. including an isolate from India (for more details. Alavi Y. Waterhouse RM. 298: 129–149. 3. 9: 1–13. in P. although some putative candidates were suggested. Holt RA. Proc Natl Acad Sci U. vivax. For example.

vivax ex-vivo maturation’ was conducted from 25 August to 5 Sept. Dr V. R. B. Mr N. Chandigarh introduced the speakers.M.K. • A workshop on Malaria and other vector borne diseases was organized for medical officers of Municipal Corporation of Delhi on 3 December 2008. R. Dr V. Mr M. Katoch felicitating Prof. Ganguly. Dash Symposia/Workshops/Trainings Organized • A workshop was held for developing ‘scientific skills’ among young researchers from 18 to 20 August 2008. Ahmedabad on 17 October 2008. It was facilitated by Drs Steven Sullivan and Jane Carlton. Sharma. Dr V. for clinicians on 27 September 2008. Dr V. Ganguly. Faculty members were Dr Bruce Russell of the Singapore Immunology Network and A*STAR. Rajamani.L. Prof.P.K. • Training was organized on Treatment of malaria for resident doctors of Department of Community Medicine.N. Prof. National Centre for Cell Science delivered the Annual Day lecture.C. Prof.C. Director. Prof. Katoch. ICMR were the guests of honour.Institute’s Activities Annual Day Celebrated The institute organized its annual day on 26 November 2008.M. • A seminar was organized on National Malaria Drug Policy for Post Graduate students of Community Medicine Department. Ministry of Health and Family Welfare. Mishra delivering the Annual Day lecture.M. Dr S. A. • A workshop on ‘P.K. Mahajan. Dash and Mr M. NHL Medical College. Mishra. N. Dr Katoch emphasized the need of translational research that would help to improve diagnosis and treatment of malaria in India. Prof. Secretary. On the dias. N. Bose INSA Research Professor and Emeritus Professor. • NIMR organized a symposium on Malaria and dengue in collaboration with the Janakpuri Chapter of the Indian Medical Association and Municipal Corporation of Delhi. M. Dr Sarala K. Mr Sanjeev Dutta. Katoch. Dr V. New York University School of Medicine. On this occasion. employees completing 25 years of service were felicitated. Translational Health Science & Technology Institute and Former DG.K. Singapore apart from scientists of NIMR. Pattanayak. Subbarao.C.C. Kalra and other dignitaries were also present in the function. Ahmedabad in collaboration with Vector Borne Disease Control Programme in India from 23 to 29 July 2008 at the Institute. Department of Health Research. S. Prof. A. ICMR. Mahajan.J.P. PGIMER. Senior Deputy Director General (Admin) and Mr Sanjeev Dutta.P. and Director General. Rajamani.M. Katoch addressing the audience Dr G. January 2009 5 . Financial Advisor. Medical College. 2008. distinguished Biotechnology Fellow & Advisor. Pillai. Dr G. Indian Council of Medical Research presided over the function.

Research Advisory Comittee meeting of Vector Biology held on 24 December 2008 at Delhi Research Advisory Comittee meeting of Parasite Biology held on 24 December 2008 at Delhi Scientific Advisory Committee (SAC) of NIMR held on 25 December 2008 at New Delhi Research Advisory committee of Integrated Disease Vector Control Project held on 17 December 2008 at Chennai Awards Received • Dr V. Pattanayak. falciparum. She was awarded prestigious travel fellowships from the Journal of Cell Sciences. Parasite Biology and Vector Biology and Control were held on 24 December 2008 under the chairmanship of Dr S. The members of the committees appreciated the progress made by NIMR in recent years. R. Uttarakhand held from 3–4 December 2008. Pattanayak. Roorkee. Dehradun. M.Institute’s Activities Scientific and Research Advisory Committee Meetings Organized The Research Advisory Committee meeting of the Integrated Disease Vector Controlled Project was organized at NIMR Field Unit.C. Dr G.K. 6 Plasmodium . Prerana Sethi and Ms. Munich. Germany. The meeting of the 29th Scientific Advisory Committee was held at the NIMR campus on 25 December 2008 and was chaired by Prof.C. Dehradun. Sharma Oration Award at the Symposium on ‘Recent advances in vector biology & control’ at DAV College. Munich. Kumkum Mishra were awarded Young Scientist Awards for their best Posters in Chemistry and Environment Science respectively in 3rd Uttarankhand Science Congress held at IIT.Prerana Sethi and Mr. The Research Advisory Committee meeting of Epidemiology. She was also awarded Geprufte wissenschafliche Hilfskraft fellowship from LMU.K. Pillai respectively. Germany for three months to study the selective forces operating in and around pfcrt in P. • Ms. UK and Boehringer Ingelheim Fonds. • Ms.P. Mahajan. • Ms. Mishra and Prof. Gaurav Verma were awarded Young Scientist awards for Oral and Poster presentations respectively at the Symposium on ‘Recent advances in vector biology & control’ at DAV College. Chennai on 17 December 2008 under the chairmanship of Dr S. Gauri Awasthi visited Ludwig Maximilian University.K. Dua was felicitated with Dr V. Uttarakhand held from 3–4 December 2008.

Bhat J. Dash AP. Kauth CW. Sharma D. Dutta S. Mishra SK. Pediatr Perinatal Epidemiol 2008. Dash AP. 24: 315–8. domain I+II of AMA-1 ectodomain. Wiegand RC. Somashekar U. Biswas S. Lander ES. Chitnis CE. Udhayakumar V. Immunogenicity of a recombinant malaria vaccine candidate. Bhatt RM. Malaria in India: challenges and opportunities. Tiwari SN. Trans R Soc Trop Med Hyg 2008. 24. Aug 6 [Epub ahead of print]. Rosen D. Singh MP. Pandey AC. 6. Wirth DF. Karforma C. 33 (3): 333–6. 9. Insecticide treated nets . Trop Med Int Health 2008. Anvikar A. Sadacharam K. India. Srivastava HC. 29. 17. Upadhyay AK. Sharma YD. 128: 22–5. 3. Dua VK. Kiwanuka GN. International J Infect Dis 2008. Haque MA. Nagpal AC. Singh N. Dash AP. Dash AP. India. Burden of cerebral malaria in central India (2004-2007). Prakash. Indian J Med Res 2008. Observation of sporozoites in naturally infected sibling species of Anopheles culicifacies complex and variance of An. Singh S. 46:3759–65. Pillai CR. Genome Biol 2008. Dash AP. Phytother Res 2008. India. India. J Am Mosq Control Assoc 2008. Dash AP. vivax: So similar yet very different (Lancet Infectious Diseases) An open label randomised study of Dihydroartemisininpiperaquine for uncomplicated malaria in India. S. Effect of Candida infection on outcome in patients with perforation peritonitis. Dash AP. Nagpal AC. Dash AP. Plasmodium falciparum and P. Dash AP. Ukey M. Antibody responses to the merozoite surface protein-1 complex in cerebral malaria patients in India. J Clin Microbiol 2008. Vaccine 2008. Building small dams can decrease malaria: a comparative study from Sundargarh District. Uday Somashekar. First Response® Combo Malaria Ag (pLDH/HRP2) card test for malaria diagnosis in forested belt of central India. Adak T. Dynamics of Anopheles culicifacies transmitted malaria in the absence of effective zooprophylaxis in a riverine settlement in Gujarat. Upadhyay AK. Shouche YS. Gates C. 4. Mittal PK. Sathyanarayan TS. Dash AP. Dixit RK. Indian J Med Res 2008. Curr Sci 2008. Anvikar AR. Valecha N. Joel PK. Shukla MM. J Ind Microbiol Biotechnol 2008. Hartl DL. et al. Subramani R. 107: 174–8. Gadge V. Biological control of mosquito populations through frogs: opportunities and constrains. Jain V. In vitro antiprotozoal activity of some xanthones isolated from the roots of Andrographis paniculata. June 21 [Epub ahead of print]. Sharma P. a primitive tribal community of central India. Birren BW. Yadav R. Rao VG. Mishra A. Gupta RB. 7. High immunogenecity and erythrocyte-binding activity in the tryptophan-rich domain (TRD) of the 74-kDa Plasmodium vivax alanine-tryptophan-rich antigen (PvATRAg74). Das PK. Prakash S. Induction of chymoelastase (Pr1) of Metarhizium anisopliae and its role in causing mortality to mosquito larvae. Genome-wide SNP genotyping highlights the role of natural selection in Plasmodium falciparum population divergence. Raghavendra K. Identification and characterization of a novel salivary cecropin cDNA from malaria vector Anopheles stephensi. Ragahavendra K. Stiles JK. Annual risk of tuberculosis infection among tribal population of central India. Malar J 2008. Alam MF. Papers in pipeline 1. et al. 12: 374–9. Field evaluation of a previously untested strain of biolarvicide (Bacillus thuringiensis israelensis H14) for mosquito control in an urban area of Orissa. Tyagi PK. Gyanchand. Tongren JE. Orissa. Gadge V. Indian J Med Res 2008. 103: 87–9. I CFAI J Biotechnol 2008. Das MK. Advait Prakash. Dash AP. Laos & Thailand (PLoS Med) Arterolane. 11. Savargaonkar DD. Alam MT. 26: 3787–94. Bhat J. Sharma A. Sharma SK. Shukla GP. 3. Anvikar AR. January 2009 7 . Tuberculous infection in Saharia. 18. do Lago Moraes S. 16 (2). vivax (Journal of Infectious Diseases) Reconstruction of Phylogenetic status of indian malaria vectors using multilocus DNA fragments (Systematic Biology) Evolutionary genetics of P. falciparum Malaria (Lancet) Extent of genetic diversity at non coding DNA of Indian P. 13. Agarwal GS. Park D. Vijayaraghavan R. Efficacy of culture filtrates of Metarhizium anisopliae against larvae of Anopheles stephensi and Culex quinquefasciatus. 2: 7–12. Houde N. Ferreira MU. 15. The usefulness of a new rapid diagnostic test. Saxena RK. J Biosci 2008. a New Synthetic Trioxolane for Treatment of Uncomplicated P. 2008. Highly sensitive amperometric immunosensor for detection of Plasmodium falciparum histidinerich protein 2 in serum of humans with malaria: comparison with a commercial kit. 25. Yadav RS. Montgomery P. Silawat N. Rajnikant. 6. Vaccine 2008. Sharma SK. Ndiaye D. Shukla M. Rai S. falciparum functional genes (PLoS Computational Biology) 16. Tiwari BK. 9: R171. 2. India. Volkman SK. Doley GC. Am J Trop Med Hyg 2008. Dash AP. 7: 121. Cortese JF. 12. Dev V. Raghavendra K. Insecticidal activity of Valeriana jatamansi (Valerianaceae) against mosquitoes. Dash AP. Mohanty SS. Awasthi G. Bhondeley MK. Kaul VK. 24: 410–4. 103: 59–66. Malar J 2008. 4. 19. 21. Chopra AK. 2. Trans R Soc Trop Med Hyg 2008. 13(11):1372–7. Haque MA. Nishant Khare. Singh N. Khare N. Gopalan N. Gopi PG. Yadav R. Rao VG. Rao VG. Dash AP. Dhamodharan R. 24: 2283–8. Shukla GP. Mboup S. 35: 1199–202. Yadav RS. Jain S. Raghavendra K. 128: 231–2. stephensi in Karnataka. Neafsey DE.technological and operational challenges. Diarrheagenic Escherichia coli and acute diarrhea in tribal preschool children of central India. Rao VK. Rao VG. Genetic variability of diurnally sub-periodic Wuchereria bancrofti in Nicobarese tribe of Nicobar group of Islands. Stange-Thomann N. Ghosh SK. 33 (4): 583–92. Anup Anvikar: Detection of bacterial DNA in cholesterol gall stones. J Biosci 2008. Indian J Gastroenterol. Daniels R. 5. Subramani R. 5. Bujard H. 23. Verma G. Bora H. 27(3):107–9. Dash AP. 14. 7: 126. Joshi H. 28. Dynamics of Plasmodium falciparum alleles in children with normal haemoglobin and with sickle-cell trait in western Uganda. Rao S. Parasitol Res 2008. 102(9):898–904. Lucchi NW. Rolling back malaria is possible. Udhayakumar V. Raghavendra K. Lukens A. 8. Lalitha PV. Rai GP. Tyndall E. Dash AP. 128: 82–3. Dua VK. 22: 40–6. Woehlbier U. 10. 27. Sharma MK. Andaman and Nicobar Islands. Saxena A. Sharma SK. World J Microbiol Biotechnol 2008. Anvikar AR. 20. International J Surgery 2008. Anvikar AR. Wares DF. J Am Mosq Control Assoc 2008. 22. Mohanty SS. Gopi PG. MJ Mendki. Sadacharam K. Prakash A. Sabeti PC. from Indian P. Dash AP. Schaffner SF. Genetic characterization and evolutionary inference of TNF-α with computational analyses. Isharaza WK. Jain V. Braz J Infect Dis 2008. Dolla CK. falciparum alleles. Das A. Ndir O. Stiles JK. 26. Singh PP. 26 (35): 4526–35. Hoti SL. Seroprevalence of sexually transmitted viruses among tribal population of central India.Institute’s Activities Research Papers Published (July–December 2008) 1. 79: 636–42. Acta Trop 2008. Singh MP. Milner DA Jr. Kumar A. 95: 82–87. Eschrich K. Singh N. Anvikar AR. Sarr O. Bharti PK. Singh Neeru. Dhananjaya Sharma.

Chennai. Goa. Sonapur and Jabalpur were shifted to new campuses. hospitals. The Liquid Nitrogen Plant was procured and installed. New Delhi-110 077. Phase-I. State-of-the-art laboratories have been established with modern equipments. IDVC project has been included as an intramural activity in 11th Plan of ICMR. Delhi. A number of research outputs of NIMR have gone into the national programme. A.P. Field units at Bengaluru. universities. Linkages of NIMR were strengthened. Jabalpur. Here are some glimpses of the same. The long pending Standing Finance Committee approval of the Integrated Disease Vector Control (IDVC) Project was obtained. Editor-in-Chief: Prof. Gwalior. Sector 8. Jiwaji University. • • • • Malaria Research Centre was renamed as National Institute of Malaria Research. The number of Ph. A. Extramural funding increased substantially. • • • • • • • Infrastructure development • NIMR was functioning from four different campuses till date. The construction of research block of NIMR in its Research papers published in indexed journals Printed and published by Prof. auditorium and other buildings. and Rani Durgavati University. IDVC field units were reorganised and two new field units were opened at Raipur (Chhattisgarh) and Ranchi (Jharkhand).D. etc. medical colleges. • • own campus is now over. Dash 8 Plasmodium . NIMR carried out collaborative research projects with a number of research organizations. programme to I.P. Agreement has been signed for construction of animal house.D. NIMR carried out a number of clinical trials with new drugs/ combinations and insecticide. LLINs.D. Dwarka. Director on behalf of National Institute of Malaria Research (ICMR). New Delhi-110 028 and published at National Insitute of Malaria Research (ICMR).D. Currently 40 candidates are persuing Ph. students increased. Ph. NIMR was a part of the IPCC document (2007) which got the Nobel Prize.P. Dash. industries. Goa University. and Printed at M/s Royal Offset Printers. Naraina Industrial Area. larvicides.Institute’s Activities Progress of NIMR (2004–08) NIMR progressed in all fronts in the last five years. etc. University. IDVC field units were strengthened in terms of manpower and infrastructure. A-89/1. NIMR scientists have also been recognized as independent guides by these Universities. Programme • • • NIMR is affiliated for Ph.