United States Department of Health and Human Services. (n.d.). Retrieved from http:// www.foodsafety.

gov/poisoning/causes/index.html !

! Berkes, J. (n.d.). Retrieved from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1773546/! ! Food-poisoning bacteria detection carrier, and method for detecting food-poisoning bacteria ! !

! Food consumption is one of the daily task in people’s lives, however, occasionally food poisoning will happen. Bacterias are one of the potential causes of food poisoning, other potential causes include viruses, parasites, molds, toxins, containments, and allegens. (foodsafetly) There are 5 known families of bacteria which causes food poisoning - Clostridium difficile, E. Coli, Bacteroides fragilis, Vibrio cholera, and Clostridium perfringens, and can be further divided into three categories based on the bacteria’s mode of infection: cytoskeleton, metalloprotease, and tight junction receptors. Each of the different methods caused the tight function within the epithelial cells be disengaged, allowing the bacteria to affect the cell lining. Cytoskeletal bacterias, Clostridium difficult and E. Coli, make changes in the altercation in the cytoskeleton of the epithelial cells. This changes involve actin degradation toxin and a secretion of skeletal proteins. Metalloprotease bacterias, Bacteroides fragilis and Vibrio cholera, involves zinc-protease affecting the tight junction’s permeability, cadherin and occludin respectively. While tight junction bacteria, Clostridium perfringens, involves the cadherin and occludin as the docking point and removes the claudins as its method of permeability. The body’s response is the cascade of Cl ions caused by cAMP-gated channels to shift the storage of water from the blood to the epithelial lumen. Understand the cause, which categorical and further which bacterial family, and the response will help determine systemically prove the causing agent. ! In conclusion, the proteins and different method require specific DNA, mRNA, and amino acid sequences. With a simple assay which isolate the bacteria’s DNA and protein sequence, can tell whether the bacterial is within our sample running a modificated ELIZA and a modified PCR. !


! With an ELIZA probing the supernatant, after isolating the circular DNA and Proteins sequence, enable the ability to seek the two key aspect of the bacterias. As these bacterias are well study, there should be a sequence of each of them, especially the DNA and tRNA sequence of the proteins. If not, a whole bacterial genome has to be sequenced quickly; the antibodies, if not developed, can be hyperdoma cell to have the taqs and receptor. This can be done with a array to detect the difference. With an Array which has the antibody within the plate. by probing in a fashion where the antibody glows will determinate if the bacteria causes food poisoning. !


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