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Model Test Paper 2013 B.Sc.-III Biotech.

Plant Tissue Culture

Attempt five questions in all, including Question No. 1 which is compulsory,selecting one question from each section. Q1. Answer the following: 1. Plant tissue culture technique is a redefined method of ________ a) Hybridization b) Vegetative Propagation c) Asexual Reproduction d) Selection 2. Polyethylene glycol is a) Fusogenic chemical b) Electro fusion stimulant c) Callus stimulant d) Differentiation stimulant
3. Somatic hybridization is achieved through a) Grafting b) Protoplast fusion c) Conjugation d) Recombinant DNA technology 4. The enzymes required to obtain wall-free / naked protoplasts are a) Cellulose and Proteinase b) Cellulose and Pectinase c) Cellulose and amylase d) Amylase and Pectinase 5. The first transgenic crop was a) Pea b) Tobacco c) Flax d) Cotton 6. A(n) __________ is an excised piece of leaf or stem tissue used in micro propagation.

a) micro shoot





a) b) c) d)

b) medium c) explant d) scion Protoplasts can be produced from suspension cultures, callus tissues or intact tissues by enzymatic treatment with a) cellulolytic enzymes b) pectolytic enzymes c) both cellulolytic and pectolytic enzymes d) proteolytic enzymes Which of the following is considered as the disadvantage of conventional plant tissue culture for clonal propagation? a) Multiplication of sexually derived sterile hybrids b) Less multiplication of disease free plants c) Storage and transportation of propagates d) Both (b) and (c) What is meant by 'Organ culture? a) Maintenance alive of a whole organ, after removal from the organism by partial immersion in a nutrient fluid b) Introduction of a new organ in an animal body with a view to create genetic mutation in the progenies of that animal c) Cultivation of organs in a laboratory through the synthesis of tissues d) The aspects of culture in community which are mainly dedicated by the need of a specified organ of the human body Which method of plant propagation involves the use of girdling? Grafting Cuttings Layering Micro propagation 10X1=10 10

Q2 Define the components and their composition of plant tissue culture media. Q3 Define the following: a) Surface sterilization technique b) Somatic embryogenesis Section - B Q4 Explain in detail the process of isolation and culturing techniques of single cell.



Q5 Describe in detail the process of isolation of somaclonal variants along with their application. 10

Section - C Q6 Describe the process micropropagation. Explain in detail along with its applications. 10 Q7 Explain in detail the steps involved in haploid production along with its major applications. 10 Section - D Q8 Explain the process of protoplast isolation, culture and its fusion for the formation of somatic hybrids. 10 Q9 How disease free plants can be produced with the help of tissue culture. 10