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SWSCC Educational Seminar & Suppliers Day August 23, 2012 - Las Colinas
quest for eternal life immortality alchemists hunted in vain for the rejuvenating Philosopher’s stone
Who is Unipex?
External/Internal factors :
pollution, foods, alcohol, tobacco, emotions…
Strategies to reverse skin aging Anti‐aging cream Skin Aging Plastic surgery Make‐up and photoshop .
scaly skin Increase in skin fragility (barrier function) Increase in roughness. wrinkles Laxity Easy bruising Decrease immune function … Mature skin AGING .Physiological changes with age Young skin Hyper‐ or hypopigmentation Seborrheic keratoses Dry.
. maturation Proteoglycans synthesis Dermal Epidermal junction Dermis/epidermis cohesion Flat Dermis level +++ Low +++ ECM component (collagens.) MMPs activities Immune functions Hypodermis level +++ Subcutaneous fat AGING . elastin. Mc: proliferation.Skin changes with age Flaky Thick +++ SC Epidermis DEJ Dermis Hypodermis +++ Wavy +++ Stratum corneum Epidermis Compact Thin Thickness = proper skin barrier Keratinocytes.
.Our strategy to reverse skin aging Flaky Thick +++ SC Epidermis DEJ Dermis Hypodermis +/.Progerin +++ Wavy +++ Stratum corneum Epidermis Compact Thickness = proper skin barrier Thin Keratinocytes.) MMPs activities Immune functions Hypodermis level +++ Subcutaneous fat +++ Progerin AGING . maturation Proteoglycans synthesis Dermal Epidermal junction Dermis/epidermis cohesion Flat Dermis level +++ Low +++ ECM component (collagens. Mc. elastin. proliferation.
2011 This process of aging gallops to about seven times the normal rate .Structural aging due to senescence marker: progerin 1 Progerin as a new marker of aging • Progerin is an altered form of the Lamin A protein (nuclear intermediate filament protein) • Accumulation of progerin in nucleus • Originally associated with the Hutchinson‐Gilford Syndrome (= Progeria) Progerin and acceleration of aging • Accumulation of progerin induces various defects in nuclear structure and functions • Alterations cell cycle progression caused by progerin known to accelerate human aging Lamin A/C From Cao et al.
Progerin in healthy cells The same molecular mechanisms responsible for premature aging in progeria act at low level in healthy cells 1 • Progerin is expressed in healthy cells (fibroblasts and terminally differentiated keratinocytes mainly) and accumulated with age (3% per year) • Cell nuclei from old individuals acquire defects similar to those of progeria patient cells From Scaffidi et al. 2008 9 years old 96 years old Progeria • Progerin has been linked to physiological ageing as presence of progerin is concomitant with alterations of the dermal compartment such as disorganized connective tissue .
Results are given in pg of progerin per µg of proteins 22% inhibition progerin production TT‐2 peptide TT‐2 peptide decreases significantly the production of progerin. an enzyme inhibitor 1 Focus on biomimetic peptide derived from structural proteins and enzymes implicated in the maintenance of Protocol • Mature human dermis fibroblasts were incubated for 96 hours with TT‐2 peptide • The progerin was quantified using an ELISA methodology.TT‐2 ‐ a biomimetic structural peptide dermal structure and connective tissue organization TT‐2 (INCI: F3‐Acetyl Tripeptide‐2) : 3 amino acid peptide derived biomimetic of Elafin. attesting its efficacy on the senescence process .
Effect of TT‐2 peptide on dermal structure and connective tissue organization 1 MMP‐1. MMP‐3 & MMP‐9 inhibition Syndecan Dermis contraction Elastase. collagenase MMPs ‐2 peptide control TT Progeline™ TT‐2 peptide control Progeline™ +27% collagen contraction Progerin T0 After24h .
Effect of TT‐2 peptide on dermal structure and connective tissue organization 1 Control + Elastase TT‐2 peptide + Elastase + Progeline™ 100% inhibition elastase Syndecan Dermis contraction Elastase. collagenase MMPs Control + collagenase TT‐2 peptide + collagenase + Progeline™ Elastin fibers Progerin Collagen fibers 43% inhibition collagenase .
collagenase MMPs TT‐2 peptide induces several biological responses at the dermis & epidermis levels Progerin 13 .Effect of TT‐2 on dermal structure and connective tissue organization 1 By modula ng senescence process (↓progerin). Syndecan Dermis contraction Elastase.
Laxity .1 Clinical Studies Firmness.
Clinical evaluation of anti‐sagging effect on jawlines 1 Protocol • • • • 10 healthy volunteers : 54 ‐64 years old with aged skin Formulation: 2% TT‐2 peptide emulsion cream or placebo Application: split‐face and split‐neck 2X/day for 56 days Measurement of the volume of the jawlines of the face by in vivo fringe projection profilometry TT‐2 peptide 2% 80% of volunteers had an improvement TT‐2 peptide decreases significantly the volume of the jaw lines after 28 and 56 days .
elasticity and viscoelasticity after 28 days . slackening skin) Formulation: 2% TT‐2 peptide emulsion cream or placebo Application: split‐face and split‐neck 2X/day for28 days Measurements are performed on both cheeks (diameter probe = 2mm) by cutometry Improvement up to: • 93% of cutaneous elasticity • 82% of cutaneous firmness • 42% of cutaneous viscoelasticity TT‐2 peptide 2% TT‐2 peptide increases significantly firmness.Clinical evaluation of firmness & elasticity Cutometry Protocol 1 • • • • 13 healthy volunteers (54 ‐ 66 years old) with saggy skin (loss of firmness.
Clinical evaluation of firmness & elasticity Major building effect over time! D0 1 TT‐2 peptide Slight improvement .
Summary – TT‐2 peptide effect Firmness. collagenases Progerine Reverse effect of TT‐2 peptide . Elasticity Laxity 1 Syndecan Dermis contraction Elastase.
elastin. maturation Proteoglycans synthesis Dermal Epidermal junction Dermis/epidermis cohesion Flat Dermis level +++ Low +++ ECM component (collagens. proliferation. Mc.) MMPs activities Immune functions Hypodermis level +++ Subcutaneous fat ..Our strategy to reverse skin aging Flaky Thick +++ SC Epidermis DEJ Dermis Hypodermis +++ Wavy +++ 1 Compact 2 Stratum corneum Epidermis Thickness = proper skin barrier Thin Keratinocytes.
Structural aging due to lipoatrophy Young skin Full fat compartments 2 Mature skin Deflated fat compartments Nasolabial wrinkles .
which means ”stimulation” and the first mention of the term hormesis in the open scientific literature was published in 1943 - + + + + • Concept launched by Givenchy with Vax’in (2010) : This vaccine‐like serum is formulated with a unique complex capable of generating micro‐stimuli. which encourage the skin's cells to produce their own age‐defying protein HSP70 .Hormesis concept 2 • Hormesis : homeostatic and adaptative beneficial response of cells to microstress exposure in order to correct a physiological imbalance • The word “hormesis” comes from the greek root “hormeticos”.
Lipohormesis™ – New proprietary patented concept 2 • Lipohormesis™ = hormesis applied to adipose tissue • Various stresses in the adipose tissue are able to stimulate its expansion through the activation of Hypoxia Inducible Factor (HIF‐1α). a transcription factor Mature skin + HIF‐1α Adipogenesis activation (adipocyte differenciation) Activation of adipocyte anabolism Lipolysis Inhibition (free fatty acid release) Lipogenesis activation (triglyceride synthesis) Young skin Adipose tissue growth Reverse aging Wrinkle reduction Plumper skin .
• Polysaccharide‐coated encapsulation: polysaccharides (stearoyl inulin) inserted in the phospholipid membrane = protective coating • Ionosome™ benefits: ‐ Resistant delivery system ‐ High penetration capacity to target adipocytes ‐ Higher efficacy L-ornithine Lipophilic side C18 C18 Hydrophilic side C18 C18 L‐ O . encapsulated in a Ionosome™ 2 • Ionosome™: new generation of delivery system (proprietary patented).L. ‐ A lipofilling‐like alternative • L‐O (INCI: Ornithine (and) Phospholipids (and) Glycolipids): L‐ornithine. an amino acid obtained by biotechnology from vegetable starch.O.
02% L‐O was added on adipocytes culture • Genic expression was evaluated through cDNA methodology Results Adipofill™ ↑ HIF‐1α ↑ genes involved in adipogenesis ↓ genes involved in lipolysis 2 ↑ genes involved in lipogenesis Adipocyte differenciation Triglyceride synthesis Free fatty acid release L‐O reproduces lipohormesis phenomenon and mimics lipofilling result by increasing fatty tissue volume with a global action . • 0.Effect of L‐O on the activation of HIF‐1and adipocyte anabolism Protocol • Human preadipocytes were isolated from biopsies and cultivated for differentiation.
Lipid storage enhancement – lipolysis Protocol 2 • • Human adipocyte obtained from a piece of surgical resection were incubated with 0.05% and 0.2% L‐O Free fatty acids (NEFA) were quantified in the media using a spectrophotometric method and the adipocyte size was evaluated through the measurement of their average diameter after staining with red oil (specific for lipids) L‐O → Increase in size L‐O → Increase in lipid content L‐O inhibits lipolysis and decreases the release of fatty acids from adipocytes by increasing lipid storage .
2 Clinical Studies Nasogenian fold .
• Formulation: 2% L‐O emulsion cream or placebo • Application: split‐face and split‐neck 2X/day for 56 days • Measurement of depth and roughness by profilometry and evaluation of smoothness by a dermatologist Reduction up to: • 27.Reduction of nasogenian fold 2 Protocol • 20 healthy volunteers (40 ‐ 60 years old) with nasogenian folds.6% of wrinkle depth 85% of volunteers had an improvement L‐O 2% L‐O 2% L‐O strongly reduces the appearance of nasogenian fold after only one month .5% of roughnesss • 37.
Reduction of nasogenian fold 2 D0 D60 L‐O strongly reduces the appearance of nasogenian fold .
Summary – L‐O effect 2 Nasogenian fold HIF‐1α Adipogenesis Lipogenesis Lipolysis Adipose tissue Reverse effect of L‐ O .
1 TT‐2 peptide By modula ng senescence process (↓ progerin). L‐O targets HYPODERMIS restructuration for a lipofilling effect and nasogenian fold reduction 1 + 2 La Spirit Cream …for a synergistic efficacy 4 . TT‐2 peptide targets DERMIS restructuration for a remodelling effect and wrinkles reduction 2 L‐O By inducing lipohormesis™ (↑ HIF‐1).
softness) & comfort Sodium Acrylates Copolymer (and) Lecithin: Brings a light texture to the emulsion . SOPHISTICATED AND BIOEFFICIENT ACTIVE INGREDIENTS AND DELIVERY SYSTEMS TT‐2 peptide: Improves firmness & reduces wrinkles and sagging L‐O: Reverses age‐related lipoatrophy to plump the skin DELICIOUS TEXTURES THAT DELIVER EXQUISIT SKIN FEEL Hydrogenated Lecithin (and) C12‐16 Alcohols (and) Palmitic acid: Brings the phospholipids touch (coolness.La Spirit Cream UNIQUE.
La Spirit Cream Tested by 62 women (45 to 70) twice a day for 28 day SKIN BENEFITS The product is pleasant to apply The texture is smooth The skin feels softer The skin feels suppler The skin seems hydrated The skin is more luminous The skin looks more uniform The signs of fatigue are less visible The skin texture looks improved The skin feels firmer The skin have a healthy glow The skin looks visibly smooth out AFTER 1 WEEK 100% 96% 92% 86% 85% 71% 67% 69% 67% 63% 56% 51% AFTER 4 WEEKS 100% 97% 97% 91% 87% 73% 79% 75% 75% 73% 72% 70% 87% 89% 84% 73% 77% Compared to Iris historical result on anti‐aging consumer tests 2567 tests IRIS DATABASE* .
Conclusion Focus on structural aging • Cellular aging with a progressive dysfunction of the cell metabolism (progerin) • Cutaneous aging characterized by a loss of elasticity. appearance of wrinkles… • Structural aging due to a lipoatrophy (loss of adipose tissue) Reverse effect of TT‐2 peptide & L‐O Young Skin Mature Skin .
Thank You ! Jeanne Calment (1875 – 1997) Living to the age of 122 years = 44 724 days .