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Table 4.3 Showing positive growth over Pseudomonas isolation media.

Positive growth of the samples
Station Road Sample no.1 (New Cake House), Sample no.4 (Krishna Bakery)

Rama Mandi

Sample no.1 (Flora Bakery), Sample no.2 (Gurunanak Bakery) and Sample no. 4 (Ashwani Confectioner)

Jyoti Market

Sample no. 1(Friends Bakery), Sample no.3 (global Bakery Shop) and Sample no.4 (Gulshan Bakery)

Model town

Sample no.1 (Prakash Bakery)

Nakodar Chowk

Sample no.1 (Fancy Bakery) and Sample no.3 (DashmeshBakery)

Sample no.2 Station road

Sample no.4 Station road

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1 Showing bacterial growth of Samples over the pseudomonas selective media collected from different areas.2 Rama mandi Sample no. Preparation of Pure Culture for Pseudomonas aeruginosa For preparing pure culture single colony has been isolated and then suspended into the nutrient broth. 11 .3 jyoti Market Sample no.1Nakaudar Chawk Sample no.4 Jyoti Market Sample no.4 Rama mandi Sample no.Sample no.1 Model Town Sample no.-3 Nakaudar Chawk Fig4.1 Jyoti Market Sample no.1 Rama mandi Sample no.

-3 Jyoti Market Sample no.-2 Rama Mandi Sample no.-1 Rama Mandi Sample no.-4 Rama Mandi Sample no.-1Jyoti Market Sample no.-4 Station Road Sample no.-2 Station Road Sample no.Gram’s staining Pseudomonas aeruginosa is a gram negative bacteria Sample no.4 Jyoti Market 12 .

Fig 4.3Showing positive results of Gelatin Hydrolysis 13 .-1Nakaudar Chawk Sample no.-3 Nakaudar Chawk Fig 4.Sample no.-1Model Town Sample no.2All figures showing gram’s negative results Biochemical Tests Biochemical tests has been done for cross checking the presence of this bacteria Gelatin Hydrolysis It gives positive result on gelatin hydrolysis which had been visualised by liquefaction of gelatine agar.

coli Growth on selective media Out of different isolated bacteria some were found to be grown on the EMB agar selective media Table no.Citrate Utilization Test Colour changing from green to blue indicates that Pseudomonas aeruginosa were present in the sample. 4.4 showing positive growth over the EMB media 14 . Fig 4.4Showing positive results of Citrate Utilization Test Confirmatory test for E.

4 Model Town 15 .1 Model Town Sample no.-2 Rama Mandi Sample no.2 Model Town Sample no.2 Station Road Sample no.2 Jyoti Market Sample no.-3 Station Road Sample no.1 Jyoti Market Sample no.4 Jyoti Market Sample no.Sample no.-4 Rama Mandi Sample no.3 Jyoti Market Sample no.

Fig.3 Model Town Sample no.Sample no. 4. After Gram’s staining 14 samples were found as negative bacteria 16 . coli For preparing pure culture single colony has been isolated and then suspended into the nutrient broth.6 showing gram negative bacteria by doing Gram’s staining. Preparation of Pure Culture for E. coli is a gram negative bacteria.5 Showing bacterial growth of Samples over the EMB media collected from different areas. Gram’s Staining E.3 Nakaudar Chawk Fig 4.4 Nakaudar Chawk Sample no.

7 Showing positive results of IndoleTest MethylRed-VogesProskauer TEST 17 .Biochemical test Biochemical tests has been done for cross checking the presence of this bacteria Indole Test Fig 4.

8 Showing positive results of Methyl RedTest Fig4.9 showing negative results of Voges Proskauer Test for E. coli Citrate Utilization Test Fig 4.10 showing negative results of Citrate Utilization Test for E.coli 18 .Fig 4.

11 showing minimum growth over the NA agar plates from biscuits samples Fig 4. Results Fig 4.13 showing no growth over the Pseudomonas isolation agar plates from biscuits samples 19 .Comparison with another bakery product (Biscuits) For observing which part of the pastry is more contaminated compared with another bakery product biscuits.Parley G biscuits were chosen and the same protocol were followed as done in the case of Pastry sample . For this .12 showing no growth over the EMB agar plates from biscuits samples Fig 4. cream and bakery part of the pastry were separately observed for bacterial growth.Further.

For Pseudomonas aeruginosa Name of Area Positive growth over Positive growth over the media from cream the media from bakery part part Sample no.15 showing positive growth over the Pseudomonas isolation agar plates.3(global Bakery Shop) --------Sample no. samples taken from the creamy part Fig4.4(Krishna ------Bakery) Sample Bakery) Sample Bakery) no.14 showing positive growth over the Pseudomonas isolation agar plates. For E. 4. Station Road Rama Mandi Jyoti Market Model town Nakodar Chowk ------ 1(Friends Sample no.coli 20 .2(Gurunanak no.5 showing comparative results of growth over the Pseudomonas isolation media from creamy and bakery part of pastry Fig4.3(DashmeshBakery) Table no. samples taken from the Bakery part.1(Fancy Bakery) -------Sample no.

3(DashmeshBakery) Table 4.Name of Area Station Road Rama Mandi Jyoti Market Model town Nakodar Chowk Positive growth over Positive growth over the media from the the media from the creamy part bakery part Sample no. it can be said that the creamy part of the pastry is more susceptible for bacterial. Fig4.6 Showing comparative results of growth over the EMB media from creamy and bakery part of pastry Fig4.17 showing positive growth over the EMB agar plates. samples taken from the creamy part.3(global Bakery Shop) -----------Sample no. samples taken from the bakery part.1(New Cake ------------House) --------------------------Sample no.16 showing positive growth over the EMB agar plates.1(Fancy Bakery) ----------Sample no. Result On the basis of this experiment. 21 .