PG -170

II Semester M.Sc. Examination, June/July 2010 (New Scheme) BIOCHEMISTRY BC - 201: Enzymology Time : 3 Hours Max. Marks : 80

Instruction: Answer question No. 1 and any five of the remaining.

1. Answer any ten of the following : a) What is mixed inhibition ? b) What is rapid equilibrium assumption ? c) What is acid-base catalysis ? Give an example.

(2x10=20)

d) With a suitable example depict ping pong mechanism using Cleland notation. e) What is transition state stabilization hypothesis ? f) Define specificity and turnover number. g) What are super reactive amino acid side chains ? Give an example. h) Give the coenzyme action of FAD in the conversion of succinate to flimarate. i) j) What is equilibrium dialysis ? Give its applications. What are multi-functional enzymes ? Give an example.

k) What is photo-oxidation ? Give an example of a photo sensitizer. 1) Illustrate the free energy profile for an enzyme catalyzed reaction.

2. a) Give the major assumptions of the MWC and KNF model for allosteric interactions. b) Discuss the rapid reaction technique employed in enzyme assay. Give its significance. (6+6)
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PG - 170
3. a) Derive the Michaelis-Menten equation for a single substrate enzyme catalyzed reaction based on steady state assumption. How is this equation linearized ? b) Give a comparative account of various reversible inhibitors. 4. a) What is active site of an enzyme ? Explain with how serine and histidine residues are identified at the active site of chymotrypsin. b) Give the mechanism of action of lysozyme. (7+5) (7+5)

5. a) With a suitable example describe the regulation of enzyme activity by covalent modification. b) What are metal activated enzymes and metalloenzymes ? Give the mechanism of action of carboxypeptidase A. 6. a) Describe the regulation of ATCase. b) Explain the mechanistic role of NAD+ and biotin. 7. Write short note on any three of the following : a) Hill equation b) Scatchard plots c) Isotope exchange studies d) Criteria of purity of enzymes. (6+6) (3x4=12) (6+6)

PG - 274
II Semester M.Sc. Examination, June/July 2010 (New Scheme) Microbiology MBP-201: MICROBIAL GENETICS Time : 3 Hours Max. Marks : 80

SECTION-A Write brief notes on any five of the following : 1. Split genes 2. Transfection 3. Primer Template 4. Insertion sequences 5. Illegitimate recombination 6. Frame-shift mutation 7. C-value paradox. SECTION - B Answer any four of the following : 8. Describe Ames Test and its application. 9. Give an experimental evidence for semi-conservative DNA replication. 10. Explain cytoplasmic inheritance with an example. 11. Illustrate the lysogenic cycle of lamda bacteriophage. 12. Describe the replication of negative strand RNA. 13. Highlight the role of Rec A in genetic recombinations.
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(5x3=15)

(4x5=20)

PG - 274 SECTION - C Answer any three of the following : 14. Give an account of the biology of plasmids and their applications. 15. Describe the mechanism of bacterial conjugation and add a note on its application. 16. Give a detailed account on cytoplasmic inheritance. 17. Explain the molecular basis of induced mutations and their role in evolution. 18. Elucidate the genome organization in eukaryotes. (3x15=45)

PG - 275
II Semester M.Sc. Examination, June/July 2010 (New Scheme) MICROBIOLOGY MBP-202 : Immunology Time : 3 Hours SECTION - A Write brief notes on any five of the following : 1. Opsonization. 2. T-cell epitope. 3. Lymph node. 4. Alloantigen. 5. Anaphylaxis. 6. ISCOM. 7. Tumor antigen. SECTION - B Answer any four of the following : 8. Explain the structure and function of thymus. 9. Describe the factors influencing antigenicity of a molecule. 10. Write the principle and applications of HLA typing. 11. What are complements ? Explain their function. 12. Explain the steps involved in immunodiagnosis of systemic lupus erythematosus. 13. Describe the mechanism of action in phagocytosis.
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Max. Marks : 80

(5x3=15)

(4x5=20)

PG - 275
SECTION - C Answer any three of the following : 14. Explain the principle, methodology and applications of indirect ELISA.

IIII III Mil I li

(3x15=45)

15. Write a detailed account of immunoglobulin genes. Add a note on their contribution to antibody diversity. 16. Discuss the types of grafts and mechanisms of graft rejection. 17. What is hypersensitivity ? Discuss the mechanisms involved in the different types of hypersensitive reactions. 18. Write a comparative analysis of production and applications of polyclonal and monoclonal antibodies.