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Producer's Guide The New Lycaeum What's New Announcements Disclaimer Leda About Leda Chemicals Taxonomy Preparations

Categories Lin s Lycaeum !omepage "orums Chat !osted #ites $oo s Contact About #ponsor Psilocybin Producer's Guide What's %elated && '!ow to produce ())) doses o* organic psilocybin in a small room e+ery wee ' ,NT%-D.CT,-N ,* a person nows what he is doing/ ,t's not di**icult to culti+ate the mycelium o* any o* the psychoacti+e psilocybin bearing mushrooms0 The mycelium is the *ibrous underground networ o* the mushroom0 The *amiliar stem and cap portions o* the mushrooms are called carpophores0 The mycelium can be readily grown in ordinary 1ason 2ars in a low cost medium in 3) to 34 days and the acti+e materials 5psilocybin and psilocin6 can be easily extracted0 This Guide shows how to carry out all o* these steps on a small or a large scale0 Complete instructions are gi+en *or locating the mushrooms/ de+eloping stoc cultures *or inoculation/ culti+ating/ har+esting/ and drying the mycelium/ extracting the acti+e al aloids/ and using the existing cultures to seed new cultures to eep an ongoing psilocybin *arm yielding a regular crop o* the hallucinogenic mycelium0 We also gi+e directions *or setting up in a small wor room a large scale psilocybin *actory which can produce at least (/))) doses o* the drug e+ery wee 0 P#,L-C7$,N AND T!8 LAW The present drug laws are a pathetic mess0 The old adage that ignorance o* the law is no excuse becomes a ludicrous statement when the laws themsel+es are rooted in ignorance0 -ne classical example o* this is the classi*ication o* the stimulant Cocaine as a narcotic0 -ne is reminded o* the 9ing in Alice in Wonderland who made up his own language as he went along with total disregard *or the accepted de*initions o* words0 , will not e+en go into the :uestion o* whether any law en*orcement agency has the moral or Constitutional right to dictate what substances we may or may not ta e into our own adult bodies0 Any modern indi+idual whose mind is not immersed in the sla+ish dung pit o* Dar Age unreasoning nows that reliable education ;; not criminal penali<ation ;; is the answer to whate+er drug problems exist0 Ne+ertheless/ we must contend realistically with the powers that un*ortunately be at this time0 They are the ones with the badges/ guns/ ga+els and

goons0 $ecause o* the a*ore mentioned ignorance o* our lawma ers it is di**icult to determine how the use o* certain hallucinogenic substances would be treated in the courts0 Possession o* psilocybin and psilocin 5misspelled in the .0#0 Code as psilocyn6 is a *elony under Title 43/ #ection ,/ 5C6 o* the .nited #tates Code 53=>) 8dition60 Psilocybe mexicana is also illegal0 There was su**icient ignorance on the part o* the lawma ers not to include the many other mushroom species containing psilocybin and psilocin0 Theoretically the possession o* any psilocybin bearing mushroom would be the same as possessing the al oloid itsel*0 $ut when it comes to prosecution it does not necessarily wor li e that0 Lysergic acid amides/ which occur in morning glory seeds/ stems/ and lea+es are also illegal/ but there is no way to pre+ent gardeners *rom raising this ornamental *lower0 ,t is also illegal *or anyone in the .#A to possess mescaline0 Peyote/ which contains mescaline/ is legal *or bona*ide members o* the Nati+e American Church when used ritualistically/ but no member may possess extracts o* the cactus or the drug mescaline0 Peyote is illegal *or non;members/ but #an Pedro and se+eral other species o* Trichocereus cacti also contain mescaline and are a+ailable *rom many legitimate cactus dealers0 ,t would be clearly illegal *or anyone to extract the acti+e principles *rom any o* the abo+e mentioned plants0 And it would be illegal *or anyone to extract psilocybin and psilocin *rom mushrooms or mycellium as described in this guide0 Anyone *ound operating a large scale mycelium *arm could +ery easily be prosecuted *or intent to manu*acture psilocybin and psilocin0 There are also many di**erent state laws which must be considered be*ore doing anything psilocybin bearing mushrooms0 There are/ howe+er many Nations which ha+e no laws regarding these substances0 We are not 2udges or attorneys and are not trying to o**er clear interpretations o* the law0 %ather we ha+e mentioned these points to gi+e some indication o* the legal pit*alls which surround the application o* the acti+ities described in this guide0 "urthermore/ laws may ha+e changed *or better or *or worse0 We/ the author and publisher/ are not recommending or endorsing the application o* the in*ormation in this guide especially in places where there are laws proscribing these substances0 We o**er this in*ormation *or the sa e o* pure nowledge because it is our Constitutional right to do so0 We do not encourage the +iolation o* any existing laws0 ",ND,NG T!8 1.#!%--1 All it ta es is one mushroom or a *ew spores and *rom this one can :uic ly de+elop a culture that will continue to produce as much psilocybin as one desires *or years to come0 $ecause the common #an 7sidro mushroom/ Psilocybe cubensis 00#inger 5"ormerly #tropharia cubensis 008arl6 is the most easily obtainable/ the most readily culti+ated/ most disease resistant/ and psychoacti+ely strongest species we ha+e geared our instructions to it's use0 There are/ howe+er/ numerous other species which contain psilocybin0 ,n case one o* these is all that is a+ailable/ we gi+e *or se+eral o* these pertinent in*ormation such a relati+e potency/ where and when to *ind specimens/ what growing conditions 5medium/ temperature/ lighting/ etc06 it *a+ors and how resistant it is to contamination0 The states/ pro+inces/ and regions named are by no means the only places where the species is to *ound0 They are places in which there ha+e been numerous reports o* *indings0 They are gi+en here to gi+e a general idea o* the type o* terrain and climate the species *a+ors0 ,n cases where ideal culti+ation temperatures and growing conditions are not gi+en much can be surmised by considering the en+ironment in which that species thri+es0 Psilocybe cubensis can be *ound in many parts o* the .nited #tates/ 1exico/ Colombia/ Australia/ and e+en #outheastern Asia0 ,t is usualy *ound growing on or near cow dung in pastures during warm rainy periods *rom "ebruary to No+ember0 There are se+eral species o* mushroom which occur on cow dung/ but *ortunately none o* these bear much resemblance to the #an 7sidro0 There are numerous toxic mushrooms growing around us0 #ome o* these could be mista en *or some o* the other psilocybin *ungi mentioned in this guide0 ,t is essential that the mushroom hunter learn to use an identi*ication ey0 A ey is a listing o* the +arious *eatures which will positi+ely identi*y a gi+en species0 ,* a specimen does not con*irm in e+ery respect to the ey/ it must not be used0 There are se+eral excellent eys to be *ound on most library shel+es0 -ne that we recommend is 9eys to Genera o* !igher "ungi by %0 #ha**er/ 4nd ed0 53=?@6 Published by the

.ni+ersity o* 1ichigan $iological #tation at Ann Arbor0 We also recommend a thorough reading o* the most help*ull boo Poisonous and !allucinogenic 1ushrooms by %ichard and 9aren !aard/ a+ailable *or AB0=( *rom Nature #tudy ,nstitute/ P- $ox 4B43/ $ellingham/ Washington =@44(0 ,t is *urther suggested that a*ter identi*ying the specimen it should be brought to an expert mycologist to be absolutely certain o* it's identity0 1any boo s on hallucinogenic mushrooms suggest a simple test *or psilocybian species which in+ol+es brea ing the *lesh o* the specimen and waiting about B) minutes *or a blueing reaction to ta e place0 The blueing is due to the oxidi<ation o* indole based substances in the *ungus0 Although it is true that most o* the psilocybin;bearing mushrooms will respond positi+ely to this test/ other species may do the same0 The poisonous 8astwood $oletus blues upon exposure o* the inner tissues to oxygen as well as does any psilocybin mushroom0 Another test which is o*ten gi+en in mushroom manuals is treating the exposed tissues with 1etol/ a chemical used in photo de+elopers0 ,t hastens the blueing o* psilocybin mushrooms/ and supposedly one can do a blueing test with it in a *ew minutes that would otherwise ta e B) minutes or more0 Any mushroom/ howe+er/ which contains indolic substances o* any sort will respond positi+ely to this test0 #ince indole;based amino acids such as tryptophan are *ound in most li+ing organisms this test is rather useless0 There is actually no *ield test *or psilocybin mushrooms0 There is howe+er/ a relati+ely simple test *or the presence o* psilocin and psilocybin that can be carried out at home by anyone who has some *amiliarity with paper chromatography0 The mushroom sample is dried/ pul+eri<ed/ and extracted into a small amount o* unheated methanol by sha ing *or hal* an hour0 A*ter the debris in the methanol has settled the paper is spotted with the top *luid in a <one about 4mm0 A*ter treating the the spotting <one with water saturated butanol *or about two hours/ the sol+ent *ront >;@ cm *rom the spotting <one would contain the psilocin and psilocybin i* they were present in the specimen0 A*ter drying the paper with a hair dryer on warm/ this outer <one is sprayed lightly with a saturated solution o* p;dimethyl;aminoben<aldehyde in alcohol and then again with 3 N hydrochloric acid0 The paper is then dried again as be*ore0 Where psilocybin is present a reddish color will de+elop0 The presence o* psilocin will be indicated by a blue;+iolet <one0 DATA -N CA%,-.# P#,-C7$,AN #P8C,8# Conocybe cyanopesD "ound *rom 1ay through #eptember usualy in dense shade scattered among mosses/ and in wet soil around bogs/ swamps/ and ditches in the northwestern .#A and as *ar east as 1ichigan0 Carpophores grow well in sphagnum moss ha+ing a range o* p! >;@0 Copelandia cyanescensD "ound in early summer through late autumn scattered/ grouped/ or clustered on cow dung/ or rich soil in "lorida and other southern states0 #pores germinate easily easily on all agar media0 -ptimum growth occurs on 18A at @) degrees "0 Carpophores can be produced on uncased compost or on rye0 Panaeolus *oeniseciiD 5Also nown as Paneaolina *oenisecii or Psilocybe *oenisecii/ and commonly nown as haymower's mushroom or har+est mushroom6 "ound in late spring and early summer/ or in Euly/ August/ and #eptember during cool/ wet seasons scattered or grouped in large numbers on lawns/ pastures/ and other grassy places throughout the .#A and in Fuebec0 Tests on specimens *ound in Washington re+ealed no psilocybin/ but eastern specimens were potent0 Panaeolus sphinctrinusD "ound in summer and autumn in small groups in *orests/ pastures/ *ields/ and roadsides almost always on cow dung in many temperate parts o* the world0 Panaeolus subalteatusD "ound *rom spring to autumn grouped or clustered o*ten in rings up to two *eet in diameter on open

ground/ *reshly manured lawns/ straw piles/ all types o* compost/ dung piles/ and roadsides in -ntario and throughout the .#A 5especially in 1assachusetts/ 1aryland/ New 7or / -hio/ 1ichigan/ Washington/ and -regon60 -ptimum growth on 18A is at @? degrees "0 ,t occasionally occurs as a weed mushroom in commercial mushroom houses0 Pholiotina cyanopodaD "ound in August through #eptember solitary to clustered on lawns in such di+erse parts o* the .#A as New 7or / Washington/ and Colorado0 Psilocybe baeocystisD "ound in autumn and winter/ solitary/ grouped/ or clustered on earth/ lawns/ mulch/ and decomposing *orest wood near scattered trees especially coni*ers ;; in western -regon and Washington0 ,t does well on Agar media at >> degrees "0 This is a potent species containing Psilocybin/ psilocin/ baeocystin/ and nor;baeocystin0 Perhaps it is because o* the latter two al aloids that it is the most +isually hallucinogenic o* the psilocybin mushrooms0 There is a report that in 3=?) a six;year old boy died a*ter eating a large number o* these mushrooms0 There has ne+er been any other indication that these al aloids are dangerous0 .ntil there is *urther clari*ication o* this :uestion/ we ad+ise that anyone using this species proceed with caution by starting with small doses and progressing gradually to larger ones0 This is especially important when using the extracted crude al aloids which may contain large concentrations o* the baeocystin al aloids0 Psilocybe caerulescensD "ound in the summer during rainy season/ grouped or clustered but rarely solitary/ mostly in shady places on soil/ sugar cane mulch/ recently turned earth or stream ban s ;; in Alabama/ northern "lorida and 1exico0 The 1exican +ariety P0 caerulescens +ar0 ma<atecorum is nown locally as 'Durrumbe'/ which means 'landslides0' There it is o*ten *ound among landslides/ or near corn or co**ee plantations0 The mycelium does best on 18A at @3 degrees "0 Thermal death occurs at =( degrees "0 ,t is almost impossible to produce carpophores on sterili<ed rye medium0 They can be grown on +egetable compost in dim light/ but the incubation period is long 5((;@( days60 Although this species is resistant to white mold it's long incubation period lea+es it prone to other diseases0 ,t is not one o* the more potent species0 Psilocybe caerulipesD "ound in summer and occasionally autumn solitary or clustered on decomposing logs and debris o* hardwood trees 5especially birch and maple6 in New 7or / New 8ngland states/ -hio/ 1ichigan/ North Carolina/ Tennessee and -ntario0 Psilocybe cubensis +ar0 cyanescens 00#inger 5*ormerly #tropharia cubensis 008arl6D "ound *rom "ebruary to No+ember in compact groups in clearings outside *orest areas/ on cow dung/ or horse dung/ in rich pasture soil/ on straw/ or on sawdustGdung mixture in 1exico/ Cuba/ "lorida and other southern states0 ,t grows well on 18A at @? degrees "0 Carpophores appear in H;@ wee s0 Thermal death occurs at 3)H degrees "0 Carpophores larger than wild specimens can be produced by inoculating +egetable compost in clay pots with agar grown mycelium/ casing with silica sandGlimestone mix/ and incubating H;? wee s in daylight at ?@ degrees "0 ,t does poorly in dar ness0 ,t is a potent mushroom and +ery resistant to contaminants0 Psilocybe cyanescensD "ound in autumn scattered/ grouped/ or clustered in woods/ on earth/ among lea+es and twigs/ and occasionally on decomposing wood ;; in northwestern .#A0 Psilocybe mexicanaD "ound *rom 1ay to -ctober isolated or sparsely at altitudes *rom H()) to (()) *eet/ especially in limestone regions/ among mosses and herbs/ along roadsides/ in humid meadows/ in corn*ields/ and near pine *orests in 1exico0

Psilocybe pelliculosaD "ound #eptember to December scattered/ grouped/ or clustered on humus and debris/ in or near coni*ier *orests in northwestern .#A and as *ar south as 1arin County/ Cali*ornia0 This is a small but potent species0 Psilocybe :uebecencisD "ound *rom summer to late -ctober scattered in shady areas at *orest edges/ on sandy soil containing +egetable debris regularly inundated by ri+er *looding/ and on decomposing wood and debris 5especially birch/ alder/ *ir/ and spruce6 in the Fuebec area0 ,t thri+es at lower temperatures than other Psilocybe species and produces carpophores at air temperatures o* HB to (= degrees "0 Psilocybe semilanceataD "ound August through #eptember o*ten in large groups on soil/ among grasses/ in clearings/ pastures/ meadows/ *orest edges/ open coni*ier woodlands/ and on roadsides ;; but ne+er on dung ;; in New 7or / northern .#A/ $ritish Columbia/ and 8urope0 Generally regarded as one o* the less potent species/ but is sometimes :uite potent0 Psilocybe strictipesD "ound in -ctober rather clustered on soil or on decomposing wood and debris/ on coni*iers and some other trees in northwestern .#A 5especially in -regon60 ,t closely resembles P0 baeocystis/ but has a longer stem0 ,t tends to be as +isually hallucinogenic as that species and probably contains the same or similar baeocystin al aloids0 Psilocybe syl+aticaD "ound in #eptember and -ctober in small compact but unlustered groups in woods on lea* mold/ debris 5especially beech wood6/ around stumps and logs/ but not usually on them ;; *rom New 7or to 1ichigan and as *ar north as Fuebec and -ntario0 This mushroom is small and is o*ten mista en *or P0 Pelliculosa0 The species discussed abo+e are only some o* the more commonly nown ones with hallucinogenic properties0 There are recogni<ed among the psilocybin bearing mushrooms H) species o* Conocybe usually ocuring in *orests/ pastures/ gardens/ dung areas/ sandy soil/ ant hills/ decayed wood/ and charcoal and ha+ing a cosmopolitan rangeI 4) species o* Panaeolus *ound on soil and dung and ha+ing a cosmopolitian rangeI H) species o* Psilocybe *ound on soil/ moss clumps and organic substrata such as dung/ rotting wood/ bagasse/ and peat ranging *rom the artic to the tropicsI and = species o* #tropharia *ound on soil/ dung and sometimes on lea* mulch and rotting wood and ha+ing a *airly cosmopolitian range0 P.%8 C.LT.%8 T8C!N,F.8 The most di**icult part o* psilocybin mushroom culti+ation is the obser+ance o* the rules o* pure culture techni:ue0 These are the sanitary code o* mushroom culti+ation0 There are usually many +arieties o* bacteria and *ungal spores in our en+ironmentI *loating in the air/ clinging to our hands and clothing/ issuing *rom our mouths with e+ery exhalation0 8xtreme measures must there*ore be ta en to eep these out o* our mycelial cultures/ which they would rapidly o+errun0 The *ollowing points should be diligently obser+ed0 Wor in a clean/ uncluttered/ dust *ree room0 ,mmediately be*ore wor wash the wor table and spray the room with disin*ectants0 #crub arms/ hands/ and nails with disin*ectant soap0 Wear simple clothing0 A *reshly cleaned short;slee+e T;shirt is ideal0 Gargle with antiseptic mouthwash and co+er the mouth and nose with a clean cloth or disposable surgery mas 0 Co+er the hair with a surgical cap or shower cap0 Allow no dra*ts in the room0 close all doors and stu** all door 2ambs0 Let no *lies/ animals/ or unnecessary people in the room0 Let only sterili<ed e:uipment touch the medium or inoculum0 Don't lean o+er your wor 0 A+oid all swi*t mo+ements that may cause a dra*t0 ,* possible ha+e a hood constructed around the wor table or a screen or curtain surrounding it0 $e neat and eep all materials within reach0 9eep all e:uipment about three *eet away *rom the wor 0 Do not permit anyone to enter the room while wor is in progress0

#T8%,L,JAT,-N All utensils used in the culti+ation o* mycelia must be sterili<ed by heat be*ore use0 Glassware must be boiled in water *or B) minutes0 1etalware used repeatedly must be held in a *lame until glowing and then allowed a moment to cool be*ore ma ing contact with any cultures or specimens0 When the inoculation loop has been used to trans*er a *ragment o* mycelium it must be *lame sterili<ed again be*ore touching the next *ragment0 All medium containers must be sterili<ed a*ter the medium has been poured0 This process is nown as autocla+ing0 Containers no more than hal* *ull with medium are placed in a canning type pressure coo er0 The lids o* these must be loose enough to allow escape o* internal pressures0 -therwise the containers may crac 0 #eal the lid o* the pressure coo er0 9eep the stopcoc +al+e open0 .sing high heat bring the coo er to boiling so that thic steam comes through the +ent0 Close the stopcoc and let the pressure rise to 3(;4) pounds0 54() Degrees "06 *or B) minutes0 This should be enough to destroy any *oreign spores or li*e*orms0 Any higher temperature or longer period would cause the dextrose or maltose sugars to carmeli<e0 This would inhibit growth and psilocybin production o* the mycelium0 When the autocla+e period is up turn o** the heat and let the coo er cool to room temperature0 Do not release the stopcoc until e+erything has thoroughly cooled or the sudden change in pressure will cause the containers to boil o+er0 Discard any containers that ha+e crac ed during sterili<ation0 9eep all containers o* medium at room temperature *or three days to see i* any *oreign molds de+elop0 ,* they do occur discard the medium in the contanminated 2ars and thoroughly clean and sterili<e such 2ars be*ore using again0 1A9,NG A #P-%8 P%,NT A spore print is a collection o* spores on a *lat sur*ace0 ,t can ser+e se+eral purposes0 ,t can be used to assist identi*ication o* the specimen by obser+ing its color or i* made on a glass slide/ by studying the shapes o* the spores under a microscope0 1ycological identi*ication eys include descriptions o* spore prints and microscopic spore *eatures *or di**erent species0 #pore prints are also the standard method o* collecting spores *or later germination on agar media0 A print *rom a single mushroom cap contains millions o* spores0 1any mushroom lo+ers are now ma ing spore prints on paper *rom species a+ailable in their locales and mailing them to culti+ators in other areas where such species are not *ound0 #ecret spore exchange correspondence clubs are becoming :uite the +ogue and will probably be more common in the +ery near *uture0 A word o* caution regarding this practice should be gi+en/ howe+er0 Do not assume that spores recei+ed in this manner are *rom the species the sender claims they are0 ,* the sender has misidenti*ied the specimen and the recipient culti+ates and ingests mycelia or extractions there*rom/ the result may be disasterous0 "urthemore/ , would not put it past some anti;drug *anatic to purpose*ully disseminate spore prints o* dangerous mushrooms to amateur culti+ators0 This could result in sic ness and death *or thousands o* persons0 To ma e a spore print ta e a mushroom with it's cap *ully opened and gills exposed0 With a sharp sterili<ed blade cut o** the stem as close to the gills a possible0 Place the cap gills;down on a clean/ white sheet o* paper/ or on a sheet o* glass that has 2ust been swabbed with alcohol/ or on two or *our sterili<ed microscopic glass slides0 Co+er the cap with a clean/ in+erted bowl or bell 2ar to pre+ent drying o* the cap and intrusion o* *oreign organisms0 Let this stand as such *or 4H hours0 ,* a good spore print has not been *ormed a*ter this time/ tap the cap lightly with the *lat side o* a ni*e or spatula0 This should sha e loose many spores0 ,* the print is made on glass/ co+er it with another glass sheet immediately a*ter remo+ing the cap to pre+ent contamination0 ,* microscopic slides are used/ place two *ace to *ace and seal the edges with tape0 ,* paper is used0 *old it se+eral times so that the print is well inside0 P%8PA%AT,-N -" 18D,A PDA 5Potato Dextrose 7east Agar6D Wash 4() grams o* unpeeled potatoes and slice them 3G@ inch thic 0 Wash these se+eral times in cool tap water until the water is clear0 Drain the slices in a collander and rinse once with distilled water0 Coo the potato slices in distilled water until tender0 #train the coo ing li:uids through a *lannel cloth or se+eral layers o* cheesecloth and collect the li:uid in a *las 0 %inse the boiled potatoes se+eral times with distilled water/ add these rinse waters to the li:uid in the *las / and discard the potatoes0 Add enough distilled water to the *las to ma e one liter0 $ring the li:uid to a boil and add 3( grams o* agar/ 3) grams o* dextrose/ and 30( grams o* yeast extract0 The agar must be added slowly and care*ully to pre+ent boiling o+er0 While the li:uid is hot/ pour it into petri dishes or other culture containers0 8ach should be *illed hal* way0

PD7 5Potato Dextrose 7east broth6D PD7 broth is made in exactly the same manner except the agar is omitted0 1ason 2ars are *illed hal* way with the hot or cool li:uid0 18A 51alt 8xtract Agar6D To one liter o* gently boiling water 5distilled6 add 4) grams o* malt extract/ 4) grams o* agar 5slowly/ care*ully to pre+ent boiling o+er6/ 3)) mg o* potassium phosphate dibasic 594!P-H6/ and 3)) mg o* calcium carbonate0 While still hot pour the li:uid into the culture dishes0 8F.,P18NT 1ost o* the e:uipment described in this guide is readily a+ailable at reasonable prices0 -ne :uart si<e mason 2ars can be purchased *rom many stores including #ears *or about A40=@ a do<en0 ,* a large scale Psilocybin *arm is being set up/ a greater number o* 2ars could be obtained *rom a Wholesale outlet or bought at a discount *rom the retailer0 Pipettes/ inoculating loops/ petri dishes/ agar/ and other materials 5including pre;mixed media6 are *ound at many scienti*ic supply houses or can be ordered *rom Di*co Laboratories/ ,nc0/ Detroit/ 1ichigan H@4)30 ,* Petri dishes are not on hand/ there are se+eral other containers that can substitute0 $aby *ood 2ars 3GH *illed with agar media are excellent0 Test tubes can be *illed 3GB with hot agar medium/ stopped with cotton/ autocla+ed and allowed to cool while standing at a 3> degree angle0 These are nown as slants and permit a maximum sur*ace area0 A wooden rac can be easily constructed to hold slants at this angle0 $aby bottles with a steam sterili<er can be bought almost anywhere0 These come in sets o* nine or ten bottles0 The tip o* the rubber nipple should be cut o** and a wad o* clean cotton pulled through *rom the inside lea+ing about 3G4 inch stic ing out0 The bottles are *illed 3GB with agar medium0 A*ter sterili<ing the bottles should be ept at a 3> degree angle0 A large pressure coo er ;; the type used *or canning and 2arring ;; can be used *or autocla+ing mason 2ars o* broth medium0 #TA%T,NG T!8 C.LT.%8 .pon obtaining one or more specimens o* a psilocybin bearing mushroom one can begin to culti+ate as much o* the hallucinogen as is desired0 Any part o* the *ungus can be used *or inoculation0 ,* the spores are used/ consideration must be gi+en to the natural li*e cycle o* the mushroom0 A single cap contains millions o* spores/ and any one o* these will germinate in the medium to *orm a mycelium0 $ut this mycelium will consist o* what is nown as mono aryotic tissue0 #uch a mycelial organism will grow *or a while/ but unless it mates with another compatible mono aryotic mycelium and *orms a di aryotic structure it will e+entually perish0 To de+elop a culture *rom spores proceed as *ollowsD #crape the spores *rom the print into about 3) ml o* sterili<ed water0 #ha e well0 Add =) ml o* sterili<ed water and sha e again0 There will be millions o* spores in this solution0 !a+e ready se+eral petri dishes or other suitable containers as described pre+iously containing sterili<ed agar medium0 Li*t the lid slightly on each container and with a sterili<ed pipette or syringe place a drop o* this spore water on three or *our di**erent parts o* the agar sur*ace/ then co+er the container immediately0 Let the dish stand at room temperature *or B;( days0 %adial growths o* mono aryotic mycelium will occur at each inoculation point0 When any two mycelia ha+e grown to the point o* ma ing contact with each other mating 5somatogamy6 has ta en place and within a *ew days these united mycelia will ha+e become di aryotic organisms0 Any portion o* this mycelial tissue can now be used to seed new cultures as described later0 ,* a portion o* one o* the carpaphores gathered in the *ield is used to inoculate the agar/ mating is not re:uired0 The tissue o* mature *ungus is/ o* course/ already di aryotic0 To a+oid contamination only inner tissue is used0 Place the mushroom cap gills;down on a clean wor area at least three *eet away *rom any e:uipment0 Wipe all dirt an slime *rom the cap with a F;tip and swab it's whole sur*ace with a se+en percent iodine solution0 Pin the cap to the Table top by inserting three disecting needles at e:uilateral points0 #terili<e an K;acto blade in a *lame/ let it cool *or a moment/ then car+e the outer s in o* the mushroom0 Cut out tiny pieces o* the inner mushroom *lesh each the si<e o* a match head0 #pear each piece with the blade point/ raise the lid o* the petrie dish slightly/ press the tissue *irmly into the agar sur*ace/ and close the lid immediately0 Place all dishes so inoculated on the incubation shel* at room temperature0 The mycelium must breath as it grows/ so do not cap the lids too tightly0 When the radial growth o* the mycelia appear on the agar sur*ace 5B;( days6 these stoc cultures are ready *or trans*erring to the broth 2ars0 ,* any stoc cultures are

not going to be used immediately/ tighten their lids and place them under re*rigeration0 They can be ept this way *or about a year0 %A,#,NG C%-P C.LT.%8# -" 17C8L,A The tas now is to select the most +igorous appearing mycelia in the dishes0 This means the largest and *astest growing specimens and/ o* course/ those not contaminated by *oreign molds0 Contaminants are not di**icult to detect because their appearance di**ers greatly *rom that o* the mycelia0 1ycelia are pure white *ibrous mats sometimes ha+ing a light bluish tinge0 Contaminants may appear as rapid;growing/ tiny/ white circular spots with blue;green centers/ or as sur*ace scums or *u<<y clusters o* either gray/ blac / yellow/ green/ or blue color0 ,* any contamients appear in any o* the culture dishes/ discard those cultures0 When the dishes containing the choicest mycelia ha+e been selected the mycelia can be trans*erred *rom the agar;based stoc cultures to the li:uid broth culti+ation 2ars0 These 2ars should ha+e been prepared and sterili<ed three days be*ore trans*erring and allowed to stand at room temperature during this time to test the e**ecti+ness o* sterili<ation by obser+ing i* contaminates appear0 Discard all broths which contain growths0 The uncontaminated 2ars are now ready *or inoculation0 #pray the room and clean the wor area as described under pure culture techni:ues0 Also spray the outside parts o* the stoc dishes and culture 2ars0 Li*t the lid o* a stoc dish 2ust enough and pic up a *ragment o* mycelium with an inoculation loop that has been *lame sterili<ed and allowed a moment to cool0 Li*t the co+er o* the 2ar/ place the mycelium *ragment in the broth and co+er the 2ar immediately0 %epeat this until all 2ars ha+e been inoculated0 %e*rigerate all unused stoc cultures0 Tighten the 2ar co+ers and sha e well to disperse the inoculum throughout the broth0 This also aerates the mediumI the mycelium needs oxygen *or li*e support and growth0 Loosen the lids again and place the 2ars on the growing shel* *or 3);34 days at >);>( degrees "0 ,* other species than Psilocybe cubensis are used/ ad2ust the temperature accordingly0 8+ery 4;B days tighten the 2ar co+ers/ sha e to aerate and disperse mycelium/ reloosen the co+ers/ and return the 2ars to the shel*0 The process o* growth can be *ollowed with a saccharimeter0 The maximum growth and highest percentage o* psilocybin occurs about *our days a*ter all o* the broth's sugar content has been used up0 The mycelium should be har+ested at this time0 Any 2ars that cannot be har+ested on that day should be re*rigerated until this can be done0 !A%C8#T,NG AND D%7,NG "ilter the medium o* each 2ar through a clean *lannel cloth/ collect the mycelial material *rom the cloth/ and place it in a pyrex ba ing dish0 Do the same with each 2ar o* mycelium until each ba ing dish is about 3GB *ull with mycelia0 Dry these in an o+en at no higher temperature than 4)) deg "0 .se an o+en thermometer0 Do not rely upon the temperature indications on the o+en nob as these may +ary *rom accuracy0 Chec the ba ing dishes periodically0 When the material *irst appears to ha+e dried shut o** the heat and let the dishes stay in the o+en until it has cooled0 This ensures the e+aporation o* residual moisture0 8ach culti+ation 2ar should yield ();3)) grams o* wet mycelium0 "resh mycelium contains about =) percent water/ so this much would dry down to ( to 3) grams o* crumbly material0 8ach ba ing dish would contain a do<en or more mycelia0 8KT%ACT,-N Crumble and pul+eri<e the dried mycelial material and combine each 3)) mg o* this material with 3) ml o* methanol0 Place the *las in a hot water bath *or *our hours0 "ilter the li:uids with suction through a *ilter paper in a buchner *unnel with Celite to pre+ent clogging0 Collect and sa+e the *iltrate li:uids0 !eat the slurry 5the mush in the *ilter paper6 two more times in methanol as be*ore/ *ilter/ and accumulate the li:uids o* the three extractions0 To be certain that all o* the al aloids ha+e been extracted do a small extraction with a portion o* the used slurry and test with 9eller's reagent 5glacial acetic acid/ *errous chloride/ and concentrated sul*uric acid60 ,* there is a +iolet indication/ al aloids are still present and *urther extraction is in order0 ,n an open bea er e+aporate the li:uids to total dryness with a hot water bath or by applying a hair dryer0 $e certain that all traces o* methanol ha+e been remo+ed0 The remaining residue should contain 4(;() percent psilocybinGpsilocin mixture0 Greater puri*ication can be achie+ed/ but would re:uire other sol+ents and chromatography e:uipment and is hardly necessary0

8ach 3)) grams o* dried mycelium should yield about 4 grams o* extracted material0 This should contain at least ()) mg o* psilocybinGpsilocin mixed or about *i*ty 3) mg doses0 Theoretically psilocin should ha+e the same e**ect upon the user as psilocybin0 The only di**erence between the two is that the later has a phosphate bond which disappears immediately a*ter assimilation in the body0 ,n other words/ in the body psilocybin turns into psilocin0 Psilocybin is a *airly stable compound/ but psilocin is +ery susceptible to oxidi<ation0 ,t is best to eep the extracted material in a dry air tight container under re*rigeration0 A sac o* silica;gel can be placed in the container to capture any moisture that may enter0 D-#AG8 The standard dose o* psilocybin or psilocin *or a 3() lb person is a ?;4) mg dose0 We will *igure the a+erage dose as 3) mg0 The crude al aloid extraction process gi+en here yields a brownish crystalline powder that is at least 4( percent pure0 8ach mason 2ar should contain at least () grams o* wet mycelium0 A*ter drying this would be about ( grams o* material0 The crude material extracted *rom this should contain 4(;B) mg o* psilocybinG psilocin or roughly 4;B hits0 This yield may +ery to some extent depending upon se+eral *actors0 1any o* these species contain less o* these al aloids than dose Psilocybe cubensis and the al aloidal content o* this species may +ery in di**erent strains0 Culti+ation conditions ha+e alot to do with yield too0 !igher temperatures 5>( degrees "06 cause more rapid growth but lesser psilocybin content than do lower temperatures 5>) degrees "06 -ne must test each new batch o* extracted material to determine the proper distribution o* dosages0 Depending on the potency o* the mycelia and how well the extraction was conducted the dose may range between 4( and 3)) mg0 Also bear in mind that the dose +aries *or di**erent indi+iduals0 LA%G8 #CAL8 P%-D.CT,-N The techni:ues and procedures described in this guide can be employed to culti+ate modest supplies o* psilocybin *or personal use/ or they can be expanded to apply to the large scale production o* many thousand doses per wee 0 A small 3) x 3( *oot room with standard @ *oot ceilings can be set up to produce an unending yield o* at least ())) doses per wee 0 The stoc culture shel+es here are 3 *oot deep and ( *eet long0 8ach could hold twenty 3( cm petri dishes0 ,* shel+ing is spaced six inches apart/ there can be as many as 3? shel+es stac ed in this space0 This would allow *or up to B)) stoc culture dishes going at one time0 The crop culture shel+es can be stac ed ten inches apart/ accommodating one :uart si<e mason 2ars and gi+ing ten le+els0 With the dimensions o* the room as mentioned this much shel+ing could hold about 4@)) 2ars 5B deep and B per running *oot60 The entire room ;; walls/ ceiling/ and shel+ing ;; should be painted with a white/ glossy itchen enamel0 This is not only an important sanitary measure/ but also impro+es the e**iciency and e+en distribution o* light in the room0 Lighting should be pro+ide by a *ew ban s o* wide spectrum *luorescent tubes *airly e+enly distributed across the ceiling and turned on *or 3);34 hours regularly each day0 These are great dust catchers/ howe+er/ and must be wiped clean periodically0 The wor table should also be painted with a hard smooth/ white *inish0 ,* the table is metal/ a small/ clean cutting board must be pro+ided on which to pin down mushroom caps when disecting them0 #hel* boards on the wall next to the table may be extended abo+e the table to pro+ide space *or storage o* wor e:uipment and ready containers0 A hood should be constructed around the table to protect it *rom dust/ etc0 A *ume hood with a *lu +ent and spar ;*ree exhaust *an should be constructed o+er the extraction area to remo+e toxic and combustible methanol +apors0 8xtraction is pre*erably conducted in another room0 ,* the culti+ation room is used *or extraction while the cultures are growing/ care must be ta en that the heat *rom the extraction process does not alter the room temperature0 The *ume hood will help by carrying o** most o* the heat0 A +inyl shower curtain should be hung around the wor table to shield the area o* bree<es when anyone enters or exits the room0 Another +inyl curtain can be hung 2ust inside the entrance to ser+e as a dust trap0 A person entering the room would close the door behind him be*ore pulling the curtain aside ;; and +ice +ersa on exiting0 The *loor should be white +inyl or asphalt tile or painted white and coated with +erathane or polyurethane0 There should be no cloth or carpeting in the room except *or a supply o* clean worl clothing and surgical mas s0 The only other items in the room would be a

stool at the wor table/ a three step ladder *or reaching the upper shel+es/ and a small table on rollers on which to place 2ars and dishes when ma ing the rounds o* the shel+es0 .nless one has a large sta** o* assistants it would be impossible to inoculate 4@)) 2ars in one wor session0 A*ter getting used to the wor one could do about 3)) 2ars an hour0 The best procedure is to set up a continuous rotation o* inoculations0 Wor ing about three hours a day about 4B( 2ars could be inoculated each session0 All 4@)) 2ars could be inoculated in 34 days0 #ections o* shel+ing would be di+ided into groups o* 4B( 2ars/ and these sections would be labled with the date and approximate time o* inoculation0 The wor schedule *or culti+ation would be as *ollowsD DA7 ,N-C.LAT8 #!A98 1on Group A Tues $ Wed C Group A Thurs D Group $ "ri 8 A and C #at " $ and D #un G A/ C/ and 8 1on ! $/ D/ and " Tues , A/ C/ 8/ and G Wed E $/ D/ "/ and ! Thurs 9 A/ C/ 8/ G/ and , "ri L $/ D/ "/ !/ and E #at Commence %einoculation 5#ee Col0 (6 C/ 8/ G/ ,/ and 9 #un D/ "/ !/ E/ and L 1on 8/ G/ ,/ 9/ and A;4 Tues "/ !/ E/ L/ and $;4 etc0 etc0 etc0 etc0 !A%C8#T %8,N-C.LAT8

Group A $ $;4 C C;4 D D;4

Group A;4

This would represent the *irst 4 wee s o* the continuous culti+ation cycle0 The continuation o* this schedule is ob+iousD sha ing e+ery other day/ har+esting approximately e+ery 34 days/ and resterili<ing/ re*illing with *resh medium/ autocla+ing/ and reinoculating the 2ars liberated by the days har+est0 ,* the total number o* 2ars is 4@))/ each group would consist o* 4B( 2ars0 The same schedule could/ o* course/ be adapted to any number o* 2ars0 Drying o* the mycelia should be done within a *ew hours a*ter har+esting0 -therwise en<ymes in the material will begin to destroy the acti+e al aloids0 -nce dried the material can be stored in cool/ dar / dry place until enough daily har+ests ha+e been accumulated to do an extraction0 ,* the mycelia can not be dried right away it can be ept in a re*rigerator *or a day or two/ or longer times in a *ree<er0 1A,NTA,N,NG A P8%P8T.AL P#,L-C7$,N "A%1 "resh inoculum can come *rom stoc culture dishes ept under re*rigeration0 ,* these should become depleted/ healthy strains o* mycelium *rom the crop cultures can be used to inoculate sterili<ed agar media in dishes0 To do so sha e the crop culture 2ar +iolently to brea up the mycelium0 Then trans*er drops o* the li:uid into autocla+ed petri dishes o* unused agar medium with a sterili<ed pipette and let it grow as be*ore0 ,* this reinoculation o* stoc cultures *rom existing crops is continued o+er a long period o* time/ the strain will e+entually wea en due to what is nown as the senescence *actor0 To a+oid this alternate the media used in the stoc dishes0 That isD i* PDA is used the *irst time/ use 18A the second time and PDA again the next time/ etc 3))) 2ar L 3))) dose

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