RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES

KARNATAKA - BENGALURU - 560041
COMPLETED PROFORMA FOR REGISTRATION OF SUBJECT FOR
DISSERTATION IN M.D (AYURVEDA
!!PHARMACOGNOSTICAL STUDY AND IN VITRO
EVALUATION OF KRIMIGHNA ACTION OF THE FOLKLORE
PLANT (Melastoma malabathricum linn.) ".S.R. TO
DIARRHOEA CAUSING ORGANISMS##
B$
DR. ARCHANA HEGDE .M
P.G. Scholar
Dept .of Post Graduate Studies in Dravyaguna
K.V.G Ayurveda Medical College and Hospital,
A!ated"a, Sullia, D.K. District#$%&'(%, Karnata"a.
GUIDE CO-GUIDE
DR. ROHINI D. BHARAD"AJ M.D.(A$% DR. SUBBANNAYYA
KOTIGADDE
Professor Professor
Dept. of P.G. Studies in Dravyaguna Dept. of Micro!iology
K.V.G Ayurveda Medical College ) Hospital K.V.G Medical College ) Hospital
A!ated"a, Sullia, Kurun*i!agh, Sullia
D.K.District#$%&'(%, Karnata"a. D.K.District#$%&'(%, Karnata"a
&010-&011
K.V.G AYURVEDA MEDICAL COLLEGE AND HOSPITAL
AMBATEDKA' SULLIA-5(4)&(
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D*. A*-./0/ H1231. M
P.G. Scholar
Departent of Post Graduate Studies in Dravyaguna
K.V.G Ayurveda Medical College and Hospital
A!ated"a, Sullia, D.K District, Karnata"a #$%&'(%
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+a*iv Gandhi ,niversity of Health Sciences, Karnata"a
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K.V.G Ayurveda Medical College and Hospital,
A!ated"a, Sullia, D.K District, Karnata"a #$%&'(%
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1 re2uest you to "indly register the !elo3 entioned su!*ect in y nae for the
su!ission of dissertation to the +a*iv Gandhi ,niversity of Health Sciences,
-engaluru, for the partial fulfillent of M.D. 4Ayurveda5 in Dravyaguna.
TITLE OF DISSERTATION:
<PHARMACOGNOSTICAL STUDY AND EVALUATION OF KRIMIGHNA
ACTION OF THE FOLKLORE PLANT (Melastoma malabathricum Linn.) ".S.R
TO DIARRHOEA CAUSING ORGANISMS=
Here3ith 1 a enclosing the copleted profora for registration of the su!*ect
for dissertation.
6han"ing you
7ours faithfully,

Place8 Sullia
Date8 Dr. Archana Hegde M
&
RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES'
KARNATAKA - BENGALURU -560041
ANNE>URE ? II
COMPLETED PROFORMA FOR REGISTRATION OF SUBJECT
FOR DISSERTATION IN M.D. (AYURVEDA
1. NAME OF THE CANDIDATE : DR. ARCHANA HEGDE.M
AND ADDRESS P.G. SCH9:A+,
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). COURSE OF STUDY : A7,+V;DA VACHASPA6H1
AND SUBJECT M.D. 4A7,5 1= D+AV7AG,=A.
4. DATE OF ADMISSION : 0$.00.(/0/
TO THE COURSE
5. TITLE OF THE TOPIC : ?PHARMACOGNOSTICAL STUDY
AND EVALUATION OF KRIMIGHNA
ACTION OF THE FOLKLORE PLANT
(Melastoma malabathricum Linn) ".S.R TO
DIARRHOEA CAUSING ORGANISMS=
)
6 BRIEF RESUME OF THE INTENDED "ORK
6.1 NEED FOR THE STUDY
Since !efore the da3n of history, the plants and trees are !elieved to possess
ystical po3ers. 6his life sustaining "no3ledge has !een passed fro generation to
generation, giving rise to the practice of fol"lore edicines, 3hich is a significant
source of Ayurveda. 6he plants used in the fol"lore are usually "no3n !y the their
regional naes !y the people 3ho use the. Most of these plants have !een identified
!y their Sans"rit naes given in the Ayurvedic classics, !ut soe plants could not !e
corelated 3ith the eAact Sans"rit naes !ecause of soe confusions, and have
reained to this day as ere fol"lore drugs, though they are indigenous to this soil. 6he
present day need is to !ring such her!s again into Ayurvedic pharacopoeia.
9ne such plant is Melastoma malabathricum Linn 3hich !elongs to the faily
of Melastoataceae, a evergreen shru! found throughout the oist parts of 1ndia,
6hailand, Malaysia and other S.;. Asian countries
0,&,$
.1t is coonly !eing used !y
fol" practitioners of a!ove entioned countries for the treatent of diarrhoea
dysentery and fever
(,.
!y 3hich the anti!acterial action of the plant can !e inferred.
Diarrhoea in Ayurveda is told as aÉÑSålÉ oÉWÒûSìuÉ सससस AÌiÉxÉÉU:
3
3here qÉlSÉÎalÉ plays the ain role in the pathogenesis of the disease, 3hereas, in
odern science, diarrhoea is classified as infectious and noninfectious. 6hey !elieve
that icro#organiss li"e ;scherichia coli, Shigella spp, Salonella, Vi!rio cholerae
are soe of the ost coon agents of infectious diarrhoea.
6he present study ais at the evaluation of Kriighna action of
"3atha4a2ueous eAtract5 of Melastoma malabathricum Linn against Shigella fleAneri,
Salonella paratyphi A and Vi!rio cholera 90 in vitro.
4
6.& REVIE" OF LITERATURE
+eferences regarding Melastoma malabathricum L 3ill !e ta"en fro the
odern teAt !oo"s
%,B,C,0/
, scientific *ournals and also fro internet.
PREVIOUS "ORK DONE
0. Da"aria et al studied antinoceptive, anti#inflaatory and antipyretic
properties of Melastoma malabathricum L leaves a2ueous eAtract in
eAperiental anials. 6he study sho3ed that the leaves possesses
antinociceptive, anti inflaatory and anti#pyretic activities. 6he findings of
this study supported previous clais on its traditional uses to treat various
ailents
(. Susanti, et al separated -ioactive Constituents fro the leaves of Melastoma
malabathricum L ,6his Phytocheical study, eployed various
chroatographic techni2ues and resulted in the isolation of a series of
flavonoids, triterpenoids and al"aloids.
6.) OBJECTIVES OF THE STUDY
05 6o collect the leaves of the plant Melastoma malabathricum Linn fro the
surrounding areas of Sullia to3nship of South Kanara according to Ayurvedic
nors.
00.a
(5 6o conduct the pharacognostical and phytocheical study
'5 6o prepare the "3atha of Melastoma malabathricum Linn according to
Ayurvedic standards.
&5 6o study its "riighna activity using standard procedures.
( MATERIALS @ METHODS
(.1 SOURCE OF DATA
0. Already eAisting inforation a!out the plant 3ill !e collected fro relevant
standard teAt!oo"s and internet articles, scientific *ournals and fro fol"
edicine
5
(. 6he identity of the plant 3ill !e confired !y ta"ing the help of an eApert
!otanist and the leaves of the plant 3ill !e collected fro the surrounding
areas of sullia to3nship, according to the Ayurvedic standard rules of
collection.
(.& METHODS OF COLLECTION OF DATA
STUDY DESIGN
1. Pharacognostical study
Macroscopical and icroscopical study of the leaves of Melatoma
malabathricum Linn locally called as dodda ne""are 3ill !e carried out.

11. Analytical study of Melastoma malabathricum Linn
0. 9rgano#leptic study
(. 6o study the rasa,guna, virya,vipa"a of the plant according to the
Ayurvedic paraeters
'. Physico#cheical study
 <oreign atter
 6otal ash
 Eater solu!le eAtracts
&. Phytocheical study
 Chroatography study
111. 1n vitro study Method8
P+;PA+A619= 9< KEA6AHA ACC9+D1=G 69 A7,+V;D1C S6A=DA+D
00!

 6o 0 part of the thoroughly 3ashed and ground leaves, & parts of 3ater
3ill !e added, and heated, and 3ill !e reduced to F of the total volue
and 3ill !e used for testing the "riighna activity.
6
AGA+ D1:,619= M;6H9D
0(
 6his ethod 3ill !e adopted to deterine the iniu inhi!itory
concentration4M1C5of the leaf eAtract. 1n this ethod, specific volues of the
leaf eAtract 3ill !e incorporated into preeasured volues of olten and
cooled to $/
/
C Mueller#Hinton agar 4MHA5, 3hich 3ill !e su!se2uently
poured into standard Petri dishes. A series of plates containing varying
concentrations of the plant eAtract, and gro3th control plates 3ithout the plant
eAtract 3ill !e prepared. 6he agar 3ill !e allo3ed to solidify and then, a
standard suspension of test !acteria40/
&
colony foring units#cfu>l5 3ill !e
spot inoculated on to each plate .
ASS;SSM;=6 C+16;+1A-After overnight incu!ation of the cultures at '%
/
C the M1C
3ill !e read as the lo3est concentration of the leaf eAtract that inhi!its the visi!le gro3th of
the test !acteriu.
61M;# K1:: ASSA7 46KA5
0'
6his ethod is one of the ethods used to assess the !actericidal activity of the
antiicro!ial agents. 6he test !acteria in id logarithiic phase of gro3th 3ill !e
inoculated into several tu!es if Mueller#Hinton !roth 4MH-5 containing varying
concentrations of the a2ueous eAtract of the leaves of Melastoma malabathricum L. A
gro3th control tu!e 3ithout the plant eAtract also 3ill !e included. 6hese tu!es 3ill !e
incu!ated at '%
/
C. 6hen sall ali2uots 3ill !e reoved at specific tie intervals say (, &,
., B and (&hrs, diluted to o!tain counta!le nu!er of colonies and plated to !rain heart
infusion agar plates for colony count deterinations. 6he nu!er of !acteria reaining in
each saple 3ill !e plotted overtie to deterine the rate of "illing !y the leaves eAtract.
ASS;SSM;=6 C+16;+1A- A three or ore log
0/
reduction in !acterial counts in the
antiicro!ial suspension as copared 3ith gro3th indicates an ade2uate !actericidal
response.


(
(.)DOES THE STUDY REAUIRE ANY INVESTIGATIONS OR
INTERVENTIONS TO BE CONDUCTED ON PATIENTS OR OTHER
HUMANS OR ANIMALSB IF SO PLEASE DESCRIBE BRIEFLYB
=o
(.4HAS ETHICAL CLEARANCE BEEN OBTAINED FROM YOUR
INSTITUTION IN CASE OF (.)B

7es, clearance has !een o!tained fro the institutional ethical coittee.
C LIST OF REFERENCE
0. Gopal"rishna -hat K, <lora of ,dupi, 1ndian =aturalist 4regd5 0
st
ed. (//'8(0B
(. Sathyanarayan -hat, Mala sutthina ooli"e vaidya, Pragathi Graphics, -lore.
P8 %'.
'. Sri -rahashan"ara shastri !hishagranthna, Madhu "osha ti"a on Madhava
=idana, Chau"a!ha Sans"rith ' -havana, Varnasi.+eprint (//.. (
nd
adhyaya
6i"a on &
th
shlo"a,8 $'
&. +avindra Shara ?=atures friend@Medicinal plants of 1ndia. An
;ncyclopaedia, Daya pu!lishing House, 6rinagar, Delhi, (//'80$&
$. =arayan Das Pra*apath, ,.Kuar, Agros Dictionary of Medicinal plants,
Agro!ios, Godhpur (//' 8 (/%
.. 7oganarasihan, +egional +esearch Centre -Hlore,Medicinal Plants of 1ndia,
Vol 0#Karnata"a,Srinivasan for 1nterline pu!lishing Pvt :td.Mallesh3ara
-H:ore8 '/&
%. Krti"ar K.+, Ma*or -asu -.D, 1.C.S 1ndian Medicinal Plants, +e#printed 0CB%,
(
nd
;d, :alith Mohan !asu. Allah!ad8 0/.B
B. =ad"arni K.M, =ad"arni A.K, 1ndian Materia Medica, Vol 0, Popular
Pra"ashan Private :iited, Mu!ai, +eprinted 0CCC8 %%.
C. 6he Eealth of 1ndia, =ational 1nstitute of Science counication, CS1+, =e3
Delhi +eprinted 0CCB, Vol .8 '((#'('
C
0/. =air C.K and Mohanan, Medicinal plants of 1ndia 43ith special reference to
Ayurveda5 =ag pu!lishers, Delhi0st ;d, 0CCB8 (B&
00. a5 Sri"antha Murthy K.+., Sharangadhara Sahitha 4;nglsh 6ranslation5
Chau"a!ha 9rientalia, Varnasi, '
rd
;d 0CC%.
a5Prathaa "handa 0I$C#.(,
!5Madyaa "nanda CI(#'
0(. Hindler G< Gorgensen GH .Procedure in antiicro!ial suscepti!ility testing Ch
0' Anti icro!ial suscepti!ility 1n86eAt!oo" of Diagnostic Micro!iology '
rd
ed.Mahon C+,:ehan DC and Manuselis G 4;ditors5 Saunders, An 1print of
;lsevier , Delhi (//% 8'(B
0'. Hindler G< ,Special procedure in antiicro!ial suscepti!ility testing Ch 0' Anti
icro!ial suscepti!ility 1n. 6eAt!oo" of Diagnostic Micro!iology '
rd
ed.Mahon
C+,:ehan DC and Manuselis G 4;ditors5 Saunders ,An 1print of
;lsevier,Delhi (//%8'$
D
D SIGNATURE OF THE CANDIDATE
10 REMARKS OF THE GUIDE

11.1 NAME AND DESIGNATION OF
GUIDE
DR. ROHINI D.BHARAD"AJ M.D (A$%
Professor
Dept. 9f Post Graduate Studies 1n Dravyaguna.
K.V.G Ayurveda Medical College And Hospital
Sullia, D.K District #$%&'(%, Karnata"a.

11.& SIGNATURE AND SEAL
11.) CO-GUIDE DR. SUBBANNAYYA KOTIGADDE
Professor
Dept. 9f Micro!iology
K.V.G Medical College And Hospital Sullia,
D.K.District# $%&'(%, Karnata"a.
11.4 SIGNATURE AND SEAL
10
11.5 HEAD OF THE DEPARTMENT DR. RAJASHEKHARA. N. M.D 4Ayu5 PhD4Ayu5
Professor ) Head
Dept. 9f Post Graduate Studies 1n Dravyaguna.
K.V.G Ayurveda Medical College And Hospital
Sullia , D.K District #$%&'(% ,Karnata"a.

11.6 SIGNATURE AND SEAL
1&.1 REMARKS OF THE CHAIRMAN
1&.& AND SEAL



11