1 Annual Report 2013

Research support services 2013
2013 Seed Health Unit
Annual Report
The Seed Health Unit (SHU) is the single gateway
for safe and efcient germplasm exchange and
distributon, process incoming and outgoing rice
seeds/grains, nonseed biological materials, and
soil samples for phytosanitary certfcaton and/or
post entry clearance. In additon, SHU facilitates
the processing of incoming and outgoing other
than rice materials and transgenic rice seeds/grains
for phytosanitary certfcaton and/or post entry
clearance and assists Plant Quarantne Service in
monitoring crop health, disposals, and movements/
transfers of transgenic materials, wild rices, and
introduced materials. Furthermore, SHU ensures
that all incoming and outgoing seed shipments are
accompanied by an appropriate Material Transfer
Agreement (MTA).
Phytosanitary Certfcaton
A. Rice seeds/grains
From January to December 2013, SHU issued
547 phytosanitary certfcates covering 74,213
nontransgenic seedlots with a total weight of
4,337.41 kg and sent to 54 countries worldwide
(Table 1). By region, Southeast Asia received the
highest total number of shipments and total number
of seedlots with 228 shipments and 33,564 seedlots,
respectvely. SHU also issued 15 phytosanitary
certfcates covering 499 transgenic seedlots (
2.70 kg) sent to eight countries worldwide (Table
2). By region, Europe and Central Asia, North
America, Oceania, and Southeast Asia received
the highest number of total shipments with three
shipments for each region. However, Europe and
Central Asia received the highest total number of
seedlots with 354 seedlots (1.9 kg). The highest
number of shipment and corresponding volume
of materials sent by IRRI to diferent countries
were nontransgenic rice seeds with 462 shipments
covering 57,092 seedlots and weighing 4,001.99
kg (Table 3). The Plant Breeding, Genetcs, and
Biotechnology (PBGB) sent the highest number
of nontransgenic and transgenic rice seeds/
grains to diferent countries (Tables 4a and 4b).
Furthermore, 25 phytosanitary certfcates covering
24,840 seedlots (497.95 kg) were also issued to the
Internatonal Network for Genetc Evaluaton of Rice
(INGER)-PBGB for their nursery seed distributon
to 20 countries worldwide (Table 5). By region,
South Asia, Southeast Asia, and sub-Saharan Africa
received the highest total number of shipments with
6 shipments for each region. However, South Asia
received the highest total number of seedlots with
16,892 seedlots weighing 333.330 kg.
The diferent fungi and nematode detected
with corresponding afected seedlots and detecton
levels are shown in Table 6. Routne seed health
tests conducted on 3,335 nontreated, nontransgenic
outgoing seedlots showed that Curvularia spp.
afected 95.74% of the seedlots while Pyricularia
oryzae afected only 1.02% of the seedlots.
Nontransgenic rice seeds were cleaned of objects
for quarantne importance, tested for health, and
treated with the prescribed ASEAN standard seed
treatment for rice—52-57 °C/15 min. This was
followed by fungicide slurry treatment with benomyl
and mancozeb, both at 0.1% by seed weight, except
for countries that do not allow seed treatment.
Fumigaton with phosphine was also administered to
all outgoing seeds.
A total of 6,120 seedlots from the T.T. Chang
Genetc Resources Center (T.T. Chang-GRC) were
submited to SHU for advance seed health testng.
The diferent fungi detected with corresponding
detecton level and afected seedlots are shown in
Table 7. Routne seed health testng revealed that
Curvularia spp. afected 99.90% of the seedlots,
while Tilleta barclayana afected only 0.21%.
B. Nonseed biological materials (NSBM)
Through SHU, a total of 239 shipments covering
62,749 various nontransgenic samples were
processed for phytosanitary certfcaton and sent
to 21 countries worldwide (Table 8). In additon,
a total of 12 shipments covering 942 various
transgenic samples were processed for phytosanitary
certfcaton and sent to 7 countries worldwide (Table
9). For nontransgenic NSBM, East Asia received
the highest total number of shipments with 93
shipments. However, Europe received the highest
total number of samples with 23, 114 samples. For
transgenic NSBM, Oceania received the highest total
number of shipments and total number of samples
with 5 shipments covering 587 samples, respectvely.
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Research support services 2013
Table 10 shows the sources and nature of
transgenic and non transgenic NSBM sent to diferent
countries worldwide. For both nontransgenic and
no transgenic NSBM, Crop and Environmental
Science Division (CESD) sent the highest number of
total shipments (85) and total number of samples
(26,478). The highest number of NSBM sent was
Setaria leaf samples (8,266) from C
4
Rice Center (CRC)
followed by rice DNA (5,928) sent by PBGB.
Post-entry clearance
A. Rice seeds/grains
Ninety-three (93) incoming nontransgenic seed
shipments covering 6,813 seedlots (628.44 kg) from
21 countries worldwide were also processed for
post-entry clearance from January to December 2013
(Table 11). By region, the highest total number of
shipment and total number of seedlots came from
Southeast Asia with 54 shipments covering 3,413
seedlots and weighing 425.90 kg. In additon, fve
incoming transgenic seeds were also processed
through SHU for post-entry clearance (Table 12).
The highest total number of transgenic rice seeds
came from the Philippines (through the Golden Rice
Project) with 170 seedlots weighing 11.65 Kg. Table
13 shows that out of the 98 incoming nontransgenic
and transgenic shipments, 75 shipments (including
HRDC materials) were nontransgenic rice seeds
covering 5,723 seedlots and weighing 515.69 kg.
PBGB received the highest number of total incoming
shipments (61) and total number of seedlots (4,573)
(Table 14).
Out of the 2,784 incoming nontransgenic rice
seedlots that were visually inspected none were
contaminated with weed seeds but 3.34% was
damaged by insects (Table 15a). Furthermore, none
of the seeds were found to be with soil. In terms of
general quality, 1,973 (70.87%) were under category
3 (Table 15b).
Seed health tests on 138 incoming, nontreated
seedlots showed that Trichoconis padwickii afected
98.55% of the seedlots, followed by Curvularia
spp., 92.75%. (Table 16). Aphelenchoides besseyi
afected only 0.72% of the seedlots. The prescribed
ASEAN standard treatments were also applied to all
incoming seeds.
B. Nonseed biological materials (NSBM)
Seventy-seven incoming NSBM shipments covering
22,012 various transgenic and nontransgenic samples
from 17 countries worldwide were processed
through SHU (Table 17). The highest number of
incoming shipments came from Southeast Asia with
33 shipments covering 19,898 various samples,
among which 9,578 samples were herbarium and
insect samples. Table 18 shows that the C
4
Rice
Center received the highest number of incoming
NSBM with 23 shipments covering 1,161 samples,
most of which are transgenic materials.
Crop health inspecton
Crop health inspectons were conducted on post-
entry plant quarantne areas and in the GRC,
PBGB, CESD and GQNPC seed multplicaton plots
during 2013 dry and wet seasons at three diferent
crop stages. Table 19 shows the diferent diseases
observed with corresponding percent incidence. For
incoming materials planted during the dry season,
0.09% of the seedlings were infected with Sclerotum
seedling blight, whereas 0.58% of the seedlings
were infected with the same disease during the wet
season. During the dry season, the most prevalent
disease observed at tllering and maturity stages was
tungro, 0.69% and 0.78%, respectvely.
On the other hand, for outgoing materials
planted in multplicaton plots, Sclerotum seedling
blight was the only disease observed at seedling
stage during both seasons, with 0.25% and 0.92%,
respectvely. During the dry season, the most
prevalent disease at tllering and maturity stages
was tungro with 0.64% and 0.06 %, respectvely. On
the other hand, bacterial leaf streak was the most
prevalent disease observed during tllering and
maturity stages, with 4.12% and 4.72%, respectvely.
Material Transfer Agreement (MTA)
The diferent types of MTA issued for outgoing and
incoming transgenic and nontransgenic rice seeds/
grains from January to December 2013 are shown
in Tables 20a and 20b, respectvely. A total number
of 795 various MTAs were issued for 587 outgoing
shipments covering 99,552 seedlots (4,838.06 kg).
Of these, 464 were standard MTAs + Open MTAs
(OMTA) issued for 193 shipments covering 51,216
seedlots, weighing 3,367.41 kg. On the other hand,
98 incoming shipments covering 7,019 seedlots
and weighing 640.717Kg were accompanied by a
standard MTA.
Monitoring of disposals and movements of
transgenics
Part of SHU’s task is to coordinate with diferent
organizatonal units and assist plant quarantne
service ofcers dispose (Table 21) and move/transfer
Annual Report 2013
Research support services 2013
transgenic materials (Table 22). In 2013, a total of
4,759 various samples were disposed, most of which
were soil samples from PBGB. In the same year,
20,836 various samples were transferred/moved,
most of which were seeds from the C
4
Rice Center.
Workshops, training courses, visitors,
informaton awareness drive
SHU also partcipated in the following actvites
coordinated by the Training Center (TC), the
Natonal Program Relatons, and/or the Events,
Visitors, and Exhibit Ofce (EVEO): 1) orientaton of
on-the-job trainees, postdoctoral fellows, MS and
PhD research scholars, and interns from diferent
countries/organizatonal units; 2) Human Resource
orientaton for new employees; 3) IRRI events such
as the Ambassador’s Day (May 3) and Women
in Rice Farming (August 5); 4) training courses
such as Two-week Rice Breeding Course with 25
partcipants from various countries; Rice: Research
to Producton Course with 29 partcipants from 11
countries; Rice Producton Techniques for Research
Technicians (Africa) with 16 partcipants; Rice
Producton Techniques for Young Researchers with
16 partcipants; and Seed Certfcaton Workshop
Training with 26 IRRI partcipants.
SHU also conducted on-the-job training for four
plant quarantne ofcers and did lectures and hands-
on actvites on seed health testng methodologies,
identfcaton of seedborne organisms, and seed
treatment for Agronomy 170 students from U.P. Los
Baños.
The Unit also had the following visitors: Dr.
Fareyzul Haque Ansarey, executve director-
Agribusiness, Advanced Chemical Industries
(ACI) Limited; Dr. Valerio Tanguilig, technical and
agronomy manager, Blue Ribbon Rice, Australia; four
students from Ubon Ratchathani University, Thailand;
Dr. Aiman Rysbekova, senior research scientst,
Insttute of Plant Biotechnology and Biology,
Kazakhstan; Ms. Marilyn M. Belarmino, manager,
Genetc Recources Center, EastWest Seed Company,
Philippines; Dr. Viviana Velazquez, INIA, Chile; and
eight delegates (lawyers) from the Ofce of the
Government Corporate Counsel of the Philippines.
SHU also conducted an informaton awareness
lecture for the IRS of CESD.
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Research support services 2013
Table 1. Distributon by region and country of outgoing, nontransgenic rice seeds/grains (January to
December, 2013).
Region/country Total shipments (no.) Total seedlots (no.) Total weight (kg)
East Asia (6)
Hongkong 1 6 .050
Japan 16 1,007 6.617
Korea N 2 6 .149
Korea S 8 8,050 193.770
PROC 39 4,247 43.670
Taiwan 8 746 4.715
Subtotal 74 14,062 248.971
Europe and Central Asia (12)
Austria 4 77 3.140
Belgium 5 10 .109
Bulgaria 1 6 .080
Denmark 1 20 .075
France 2 47 .324
Germany 11 123 64.506
Netherlands 11 630 38.095
Norway 1 2 .024
Portugal 1 6 .080
Russia 2 17 .214
Switzerland 4 75 7.343
United Kingdom 11 73 2.030
Subtotal 54 1,086 116.020
Latn America (1)
Colombia 4 1,248 6.192
Subtotal 4 1,248 6.192
North America (2)
Canada 2 5 2.610
USA 21 8,887 132.902
Subtotal 23 8,892 135.512
Oceania (3)
Australia 12 4,546 57.580
Fiji 1 32 .720
New Zealand 1 19 .240
Subtotal 14 4,597 58.540
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Research support services 2013
Region/country Total shipments (no.) Total seedlots (no.) Total weight (kg)
South Asia (4)
Bangladesh 17 787 60.140
India 72 5,615 169.216
Nepal 9 406 36.640
Sri Lanka 4 84 4.905
Subtotal 102 6,892 270.901
Southeast Asia (10)
Brunei Darussalam 2 32 .430
Cambodia 1 134 10.700
Indonesia 16 3,284 77.430
Laos 3 355 11.300
Malaysia 5 85 1.819
Myanmar 13 449 142.170
Philippines 161 27,578 3,086.764
Singapore 5 41 5.235
Thailand 5 283 16.970
Vietnam 17 1,323 49.215
Subtotal 228 33,564 3,402.033
Sub-Sahara Africa (11)
Burundi 5 275 17.480
Ethiopia 1 40 2.100
Ghana 2 20 .255
Kenya 2 90 2.640
Mali 1 31 .670
Mozambique 3 963 27.200
Rwanda 1 12 .925
Senegal 6 367 8.640
South Africa 2 15 .100
Tanzania 8 834 18.444
Uganda 2 76 4.000
Subtotal 33 2,723 82.454
West Africa (1)
Benin 7 28 1.700
Subtotal 7 28 1.700
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Research support services 2013
Region/country Total shipments (no.) Total seedlots (no.) Total weight (kg)
West Asia & North Africa (4)
Afghanistan 2 18 .420
Iran 4 1,062 11.010
Israel 1 3 3.000
Turkey 1 38 .660
Subtotal 8 1,121 15.090
Grand total (54) 547 74,213 4,337.413
Table 2. Distributon by region and country of outgoing transgenic seeds/grains (January to December, 2013).
Region/country Total shipments (no.) Total seedlots (no.) Total weights (kg)
East Asia (1)
Japan 2 32 .130
Subtotal 2 32 .130
Europe and Central Asia (2)
Germany 2 310 1.800
United Kingdom 1 44 .100
Subtotal 3 354 1.900
Latn America (1)
Colombia 1 36 .036
Subtotal 1 36 .036
North America (2)
Canada 1 23 .025
USA 2 9 .464
Subtotal 3 32 .489
Oceania (1) 34
Australia 3 .056
Subtotal 3 34 .056
Southeast Asia (1)
Philippines 3 11 .085
Subtotal 3 11 .085
Grand total (8) 15 499 2.696
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Research support services 2013
Table 3. Nature of outgoing shipments with corresponding total number of shipments, total number of seedlots, and
total weight (kg) (January to December 2013).
Nature of shipment/ Destnaton Total shipments (no.) Total seedlots (no.) Total weight (kg)
Rice seeds
East Asia 45 10,457 210.169
Europe and Central Asia 45 670 96.735
Latn America 3 1,228 6.165
North America 15 212 5.785
Oceania 8 2,250 27.280
South Asia 85 5,883 224.511
Southeast Asia 213 32,520 3,332.103
Sub-Saharan Africa 33 2,723 82.454
West Africa 7 28 1.700
West Asia & North Africa 8 1,121 15.090
Subtotal 462 57,092 4,001.992
Rice four
Europe and Central Asia 1 5 .055
South Asia 1 5 .035
Southeast Asia 3 24 .300
Subtotal 5 34 .390
Wild rice seeds
East Asia 4 142 .660
North America 1 9 .035
South Asia 1 6 .020
Subtotal 6 157 .715
Dehulled rice seeds
East Asia 12 427 4.887
Europe and Central Asia 1 10 .355
Latn America 1 20 .027
Oceania 1 1,351 23.000
South Asia 1 8 .110
Subtotal 16 1,816 28.379
Rice and wild rice seeds
East Asia 13 3,036 33.255
Europe and Central Asia 5 63 .675
North America 6 8,659 129.597
Oceania 1 54 .360
South Asia 7 515 6.025
Southeast Asia 4 324 6.630
Subtotal 36 12,651 176.542
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Research support services 2013
Nature of shipment/destnaton Total shipments (no.) Total seedlots (no.) Total weight (kg)
Transgenic seeds
East Asia 2 32 .130
Europe and Central Asia 2 343 .800
Latn America 1 36 .036
North America 3 32 .489
Oceania 2 32 .050
Subtotal 10 475 1.505
HRDC seeds
South Asia 7 475 40.200
Southeast Asia 8 696 63.000
Subtotal 15 1,171 103.200
Milled Rice seeds
Europe and Central Asia 2 338 18.200
North America 1 12 .095
Oceania 4 942 7.900
Subtotal 7 1,292 26.195
Golden Rice
Southeast Asia 3 11 .085
Subtotal 3 11 .085
Polished rice and Golden Rice
Europe and Central Asia 1 11 1.100
Subtotal 1 11 1.100
Polished Golden Rice
Oceania 1 2 .006
Subtotal 1 2 .006
Grand total 562 74,712 4,340.109
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Table 4a. Sources of outgoing nontransgenic rice seeds/ grains (January to December, 2013).
Organizatonal unit Total shipments (no.) Total seedlots (no.) Total weight (kg)
C4 Rice Center
Rice seeds 2 5
.
020
Subtotal 2 5 .020
Crop and Environmental Sciences
Division (CESD)
Dehulled seeds 3 3 .168
Rice seeds 19 879 76.981
Subtotal 22 882 77.149
Grain Quality and Nutriton Center
(GQNC)
Dehulled rice seeds 1 10 .355
Milled rice seeds 7 1,292 26.195
Rice four 5 34 .390
Rice seeds 4 579 7.930
Subtotal 17 1,915 34.870
Natonal Program Relatons (NPR)
Rice seeds 7 16 35.800
Subtotal 7 16 35.800
Plant Breeding, Genetcs & Bio-
technology (PBGB)
Dehulled rice seeds 7 1,445 24.037
HRDC seeds 15 1,171 103.200
Rice and wild rice seeds 2 32 .345
Rice seeds 321 50,556 3,772.748
Subtotal 345 53,204 3,900.330
T.T. Chang Genetc Resources
Center (TTC-GRC)
Dehulled rice seeds 5 358 3.819
Rice and wild rice seeds 34 12,619 176.197
Rice seeds 109 5,057 108.513
Wild rice seeds 6 157 .715
Subtotal 154 18,191 289.244
Grand total 547 74,213 4,337.413
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Research support services 2013
Table 4b. Sources of outgoing transgenic rice seeds/grains (January to December, 2013).
Organizatonal unit Total shipments (no.) Total Seedlots (no.) Total weights (kg)
C4 Rice Center
Transgenic rice seeds 6 400 .879
Subtotal 6 400 .879
Plant Breeding, Genetcs & Biotechnology
(PBGB)
Golden Rice 3 11 .085
Polished Rice and Golden Rice 1 11 1.100
Polished Golden Rice 1 2 .006
Transgenic seeds 4 75 .626
Subtotal 9 99 1.817
Grand total 15 499 2.696
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Table 5. Distributon by region and country of outgoing rice seeds (nursery sets) sent through
INGER-PBGB (January to December, 2013).
Region/country Total shipments (no.) Total seedlots (no.) Total weights (kg)
East Asia (3)
Korea N 1 166 2.700
Korea S 1 489 9.400
PROC 1 1,173 27.010
Subtotal 3 1,828 39.110
Europe and Central Asia (1)
Russia 1 606 13.100
Subtotal 1 606 13.100
Latn America (1)
Surinam 1 120 3.700
Subtotal 1 120 3.700
South Asia (5)
Bangladesh 2 1,194 22.130
India 1 11,867 235.300
Nepal 1 777 13.000
Pakistan 1 1,298 28.500
Sri Lanka 1 1,756 34.400
Subtotal 6 16,892 333.330
Southeast Asia (3)
Indonesia 1 394 9.400
Myanmar 1 259 6.000
Vietnam 4 2,142 41.520
Subtotal 6 2,795 56.920
Sub-Sahara Africa (5)
Mozambique 1 264 4.000
Senegal 1 565 13.400
Tanzania 1 264 3.860
Uganda 2 607 10.570
Zambia 1 84 1.760
Subtotal 6 1,784 33.590
West Asia and North Africa (2)
Egypt 1 402 8.700
Iraq 1 413 9.500
Subtotal 2 815 18.200
Grand total (20) 25 24,840 497.950
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Table 6. Seedborne pathogens detected on untreated, nontransgenic outgoing seeds re-
ceived by SHU for phytosanitary certfcaton (January to December, 2013).
Pathogens Afected seedlots (%) Detecton level (%) Mean value (%)
Curvularia spp. 95.74 1 – 76 13.97
Trichoconis padwickii 89.87 1 – 82 13.72
Phoma spp. 79.73 1 – 67 6.60
Sarocladium oryzae 48.07 1 – 36 4.27
Nigrospora spp. 47.17 1 – 59 4.70
Fusarium moniliforme 24.92 1 – 14 1.52
Bipolaris oryzae 23.72 1 – 12 1.44
Microdochium oryzae 10.40 1 – 11 1.55
Tilleta barclayana 6.30 1 – 100 12.04
Aphelenchoides besseyia 2.82 1 – 51 5.73
Pyricularia oryzae 1.02 1 – 9 1.38
Based on 200 seeds/seedlot for testng (n=3,335)
aActual nematode count using sedimentaton test.
Table 7. Seedborne pathogens detected on untreated GRC seeds submited to SHU for
advanced seed health testng (January to December 2013).
Pathogens Afected seedlots (%) Detecton level (%) Mean value (%)
Curvularia spp. 99.90 1 – 72 16.50
Phoma spp. 94.05 1 – 64 6.58
Trichoconis padwickii 92.73 1 – 72 11.02
Sarocladium oryzae 38.04 1 – 59 3.89
Nigrospora spp. 28.81 1 – 28 2.37
Fusarium moniliforme 26.55 1 – 18 2.04
Bipolaris oryzae 6.29 1 – 6 1.35
Aphelenchoides bes-
seyia
2.88 1 – 60 5.15
Microdochium oryzae 1.78 1 – 3 1.23
Pyricularia oryzae 0.98 1 – 22 3.12
Tilleta barclayana 0.21 1 – 2 1.54
Based on 200 seeds/seedlot for testng (n=6,120)
a
Actual nematode count using sedimentaton test.
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Table 8. Distributon by region, country, and nature of shipment with corresponding total number of shipments and
total number of samples of outgoing nontransgenic, nonseed biological materials (January to December, 2013).
Region/country Total shipments (no.) Total samples (no.)
EAST ASIA (4)
Hongkong
DNA (bacterial isolates) 1 4
DNA (sorghum leaves) 2 8
RNA (bacterial isolates) 2 24
RNA (rice leaves) 2 13
RNA (rice seeds) 1 12
Japan
DNA (rice) 4 340
Rice seedlings (Porteresia coarctata) 1 5
Rice seedlings (wet) 1 5
RNA (rice) 2 42
Soil (ground-dried) 1 5
Soil (wet) 2 10
Korea S
DNA (plasmid) 30 216
DNA (rice) 20 148
DNA, primer (plasmid) 8 146
DNA, primer (rice) 5 94
DNA, primer (sorghum ) 1 30
China, PR
DNA (rice) 1 1
DNA (rice blast) 1 50
Insects and spiders (assorted pinned and
card-pointed) 1 909
Insects and spiders (with alcohol) 1 3,527
RNA (rice) 5 66
RNA (rice leaves) 1 24
Subtotal 93 5,679
EUROPE (6)
Belgium
Soil (air-dried) 1 6
France
Aeschynomene (seed) 2 13
Germany
Arthropods (assorted insects) 1 147
Bees (dead) 1 49
Bees (oven-dried) 2 326
Fruits and vegetables (freeze-dried/
vacuum-sealed) 1 18
Grass, rice leaves (dried) 1 108
Herbarium specimens (dried plant and leaf) 1 418
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Research support services 2013
Region/country Total shipments (no.) Total samples (no.)
Herbarium specimens (oven-dried) 1 503
Insects (assorted) 3 1,493
Insects (dead) 1 45
Insects and Rice plants (LEGATO collectons) 3 567
Living earthworms (in wet soil) 1 200
Living earthworms in wet soil (Pheretma) 1 100
Maize plant (protein extracts) 1 2
Moths and Buterfies (dead) 1 292
Parasitoids, arthropods, BPH, GLH nymphs
(assorted) 1 3,419
Plant samples (leaves (air-dried)) 1 79
Rice anther (frozen) 1 79
Rice grains, leaves, straw, plants, soil, water
(ground-dried) 1 718
Rice Leaf samples (protein extracts) 1 2
Rice plant and root samples (dried) 1 36
Rice plant straw, maize leaves and stalk,
rice roots (dry) 1 36
Rice plant straw, maize leaves and stalk,
rice roots, maize roots (dry) 2 63
Rice Root samples (dried) 1 79
Rice Root samples (frozen) 1 32
Rice spikelets and leaves (frozen) 1 495
Rice straw (decomposed) 1 516
Rice straw (oven-dried) 2 657
Rice straw and roots (dried) 1 36
Germany
Rice straw, rice leaves, maize leaves,
grass leaves (dried) 1 86
Root samples (rice) 2 56
Root slurry, soil slurry and soil cores (frozen) 1 1,880
Root slurry, soil slurry and soil cores (wet) 1 54
Root, soil, pore water (core and slurry) 1 54
Root, soil, pore water (frozen) 1 90
Soil (dried) 2 153
Soil (dried and coarse) 3 1,076
Soil (frozen) 4 80
Soil (oven-dried) 1 2
Soil (wet) 2 46
Soil and water samples (frozen) 5 970
Soil, plant and water (frozen) 1 169
Straw, leaves and water samples (rice) 1 619
Vegetables and Fruits (oven-dried) 1 57
Water samples (pore) 1 25
Annual Report 2013
Research support services 2013
Region/country Total shipments (no.) Total samples (no.)
Italy 2 266
DNA (leaf from sweet sorghum and sugar cane) 1 2
RNA and rice spikelets (frozen) 1 88
Netherlands
Rice leaf samples (ground) 1 2,930
Rice root samples (frozen) 1 8
Root samples (rice) 1 1,776
Soil (dried) 1 8
United Kingdom
Bacterial isolates (in ampoules) 1 19
DNA (rice) 4 1,951
DNA, sterilized H2O (rice) 1 96
Fungal isolates (in tubes) 1 4
Protein extracts (rice) 1 8
Rice straw (ground-dried) 2 2
Soil (dried) 1 3
Soil (dried and ground) 1 2
Subtotal 86 23,114
NORTH AMERICA (1)
USA
Azolla (Azolla fliculoides, mexicana, caroliniana) 1 13
Bacterial artfcial chromosome probe (dried) 2 8
Bacterial culture Xoo (lyophilized) 1 74
DNA (rice) 11 4,752
Plasmids (dried) 1 2
Rice Leaf samples (dried) 1 69
Rice Leaf tssues (frozen) 2 3,080
Rice seeds (young) (frozen) 1 5
Rice straw (ground) 1 1,046
RNA (rice) 1 24
Setaria (leaf samples) 1 8,266
Setaria leaf (Poaceae) (dried) 2 4,705
Soil (dry) 1 2
Sorghum seeds (seed) 1 4
Wild rice leaf tssues (frozen) 1 11
Subtotal 28 22,061
OCEANIA (1)
Australia
DNA (rice) 1 369
Parasitc wasps (insects) 1 700
Plant tssue (ground) 1 252
Rice leaf and water (ground) 1 34
Rice straw (dried) 1 360
Setaria stem (oven-dried) 1 2,736
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Region/country Total shipments (no.) Total samples (no.)
Sorghum leaves (dried) 1 281
Sorghum leaves (dried and ground) 1 135
Sorghum seeds (seed) 1 2
Subtotal 10 8,088
SOUTH ASIA (2)
Bangladesh
Taq polymerase enzymes (gene clone) 1 1
India
DNA (rice) 1 360
Rice seedlings (wet) 1 5
Subtotal 3 366
SOUTHEAST ASIA (4)
Malaysia
Antserum (RTSV, RTBV) 1 4
Philippines
Azolla (Azolla microphylla, mexicana, 3
caroliniana) 1 4
Azolla (fresh) 1 5
Bacterial culture Xoo (lyophilized) 1 26
Bacterial isolates (in ampoules and slants) 1 96
DNA (bacteria) 1 96
DNA (bacterial isolates) 1 46
DNA (fungal isolates) 1 8
Flour extract (rice) 1 249
Rice seedlings (fresh) 1
Singapore
DNA (rice) 1 575
Flour extract (rice) 3 35
Leaf tssues (rice) 1 276
Rice Leaf tssues (frozen) 1 1,915
Vietnam
Bacterial isolates (in ampoules) 1 18
Subtotal 17 3,356
SUB-SAHARA AFRICA (2)
Nigeria
Primers (SC3, ART5) 1 4
Senegal
Taq polymerase enzymes (frozen) 1 81
Subtotal 2 85
Grand total (20) 239 62,749
Annual Report 2013
Research support services 2013
Table 9. Distributon by region, country, and nature of outgoing, transgenic, nonseed biological mate-
rials shipments with corresponding total number of shipments and total number of samples (January
to December, 2013)
Region/ Country Total shipments (no.) Total samples (no.)
EAST ASIA (1)
Japan
RNA (transgenic, ground) 1 78
Subtotal 1 78
EUROPE (2)
Germany
Transgenic rice leaves (protein extracts) 1 4
United Kingdom
Metabolites (transgenic rice leaves) 1 27
Subtotal 2 31
NORTH AMERICA (2)
Canada
Transgenic rice leaves (dead) 2 50
USA
Transgenic rice leaves (dried) 1 192
Subtotal 3 242
OCEANIA (1)
Australia
DNA from rice leaves (Golden rice purifed
plant genomic) 2 458
Golden Rice (four) 1 5
Transgenic DNA (plasmid) 1 4
Transgenic rice root and shoot tssue (ground) 1 120
Subtotal 5 587
SOUTHEAST ASIA (1)
Philippines
Rice straw, rice bran (transgenic, ground) 1 4
Subtotal 1 4
Grand total (7) 12 942
18
Research support services 2013
Table 10. Sources of outgoing transgenic and nontransgenic, nonseed biological materials (January to
December, 2013).
Organizatonal unit/ nature of materials Total shipments (no.) Total samples (no.)
C4 Rice Center
DNA sorghum leaves 2 8
DNA, primer rice 2 42
DNA, primer sorghum 1 30
Maize plant protein extracts 1 2
Rice Leaf samples dried 1 69
Rice Leaf samples protein extracts 1 2
RNA rice 5 66
RNA rice leaves 3 37
Setaria leaf samples 1 8,266
Setaria leaf (Poaceae) dried 2 4,705
Setaria stem dried 1 3,219
Setaria stem oven-dried 1 2,736
Sorghum leaves dried 1 281
Sorghum leaves dried and ground 1 135
Sorghum seeds seed 2 6
Transgenic rice leaves dead 2 50
Transgenic rice leaves dried 1 192
Transgenic rice leaves protein extracts 1 4
Subtotal 29 19,850
Crop and Environmental Sciences Division (CESD)
Aeschynomene seed 2 13
Arthropods assorted insects 1 147
Azolla Azolla fliculoides, mexicana, caroliniana 1 13
Azolla Azolla microphylla, mexicana, caroliniana 1 3
Azolla fresh 1 4
Bees dead 1 49
Bees oven-dried 2 326
DNA plasmid 1 17
DNA rice 1 369
Grass, rice leaves dried 1 108
Herbarium specimens dried plant and leaf 1 418
Herbarium specimens oven-dried 1 503
Insects assorted 3 1,493
Insects dead 1 45
Insects and rice plants LEGATO collectons 3 567
Insects and spiders assorted pinned and card-pointed 1 909
Crop and Environmental Sciences Division (CESD)
Insects and spiders with alcohol 1 3,527
Living earthworms in wet soil 1 200
Living earthworms in wet soil Pheretma 1 100
Annual Report 2013
Research support services 2013
Organizatonal unit/ nature of materials Total shipments (no.) Total samples (no.)
Moths and buterfies dead 1 292
Parasitc wasps Insects 1 700
Parasitoids, arthropods, BPH, GLH nymphs assorted 1 3,419
Plant samples leaves (air-dried) 1 79
Primers SC3, ART5 1 4
Rice grains, leaves, straw, plants, soil, water ground-dried 1 718
Rice leaf and water ground 1 34
Rice leaf samples ground 1 2,930
Rice plant and root samples dried 1 36
Rice plant straw, maize leaves and stalk, rice roots dried 1 36
Rice plant straw, maize leaves and stalk, rice roots, 2 63
maize roots dried 2 40
Rice Root samples frozen 1 249
Rice seedlings fresh 1 516
Rice straw decomposed 1 360
Rice straw dried 2 2
Rice straw ground-dried 2 657
Rice straw oven-dried 1 36
Rice straw and roots dried
Rice straw, rice leaves, maize leaves, grass leaves
dried 1 86
Root samples rice 2 3,656
Root slurry, soil slurry and soil cores frozen 1 54
Root slurry, soil slurry and soil cores wet 1 54
Root, soil, pore water core and slurry 1 90
Root, soil, pore water frozen 2 153
Soil air-dried 1 6
Soil dried 6 1,089
Soil dried and coarse 4 80
Soil dried and ground 1 2
Soil frozen 1 2
Soil ground-dried 1 5
Crop and Environmental Sciences Division (CESD)
Soil oven-dried 2 46
Soil wet 7 980
Soil and water samples frozen 1 169
Soil, plant and water frozen 1 619
Straw, leaves and water samples rice 1 57
Taq polymerase enzymes frozen 1 81
Taq polymerase enzymes gene clone 1 1
Water samples pore 2 266
Subtotal 85 26,478
20
Research support services 2013
Organizatonal unit/ nature of materials Total shipments (no.) Total samples (no.)
Grain Quality and Nutriton Center (GQNC)
DNA rice 1 285
Flour extract rice 4 43
Subtotal 5 328
T.T. Chang Genetc Resources Center (TTC-GRC)
DNA rice 4 1914
Rice seedlings Porteresia coarctata 1 5
Rice seedlings wet 2 10
Subtotal 7 1,929
Plant Breeding, Genetcs & Biotechnology (PBGB)
Bacterial isolates (Xanthomonas oryzae) 1 14
Antserum RTSV, RTBV 1 4
Bacterial artfcial chromosome probe dried 2 8
Bacterial culture Xoo Lyophilized 2 79
Bacterial isolates in ampoules 2 37
Bacterial isolates in ampoules and slants 1 26
DNA bacteria 1 96
DNA bacterial isolates 2 100
DNA fungal isolates 1 46
DNA leaf from wweet sorghum and sugar cane 1 2
DNA plasmid 29 199
DNA rice 37 5928
DNA rice blast 1 50
DNA from rice leaves Golden rice purifed plant genomic 2 458
DNA, primer plasmid 8 146
DNA, primer rice 3 52
DNA, sterilized H2O rice 1 96
Fruits and vegetables freeze-dried/vacuum-sealed 1 18
Plant Breeding, Genetcs & Biotechnology (PBGB)
Fungal isolates in tubes 1 4
Golden Rice four 1 5
Leaf tssues rice 1 276
Plant tssue ground 1 252
Plasmids dried 1 2
Protein extracts rice 1 8
Rice anther frozen 1 79
Rice Leaf tssues frozen 3 4995
Rice Root samples dried 1 79
Rice seeds (young) frozen 1 5
Rice spikelets and leaves frozen 1 495
Rice straw ground 1 1046
Rice straw, rice bran transgenic, ground 1 4
RNA bacterial isolates 2 24
RNA rice 6 134
Annual Report 2013
Research support services 2013
Organizatonal unit/ nature of materials Total shipments (no.) Total samples (no.)
RNA transgenic, ground 1 78
RNA and rice spikelets frozen 1 88
Transgenic DNA plasmid 1 4
Transgenic rice root and shoot tssue ground 1 120
Vegetables and fruits oven-dried 1 25
Wild rice leaf tssues frozen 1 11
Subtotal 125 15,093
Grand total 251 63,678
22
Research support services 2013
Table 11. Origin and corresponding total number of shipments, total number of seedlots, and total weight
(kg) of incoming nontransgenic rice seeds/grains (January to December, 2013).
Region/country Total shipments (no.) Total seedlots (no.) Total weight (kg)
East Asia (3)
Japan 1 169 .450
Korea S 8 2,391 25.873
China 2 2 5.500
Subtotal 11 2,562 31.823
Europe and Central Asia (2)
Germany 2 4 .095
Portugal 1 2 .425
Subtotal 3 6 .520
Latn America (1)
Colombia 1 143 19.200
Subtotal 1 143 19.200
South Asia (2)
Bangladesh 2 11 5.200
India 12 33 56.150
Subtotal 14 44 61.350
Southeast Asia (5)
Cambodia 3 100 17.245
Indonesia 3 398 78.030
Myanmar 2 80 6.900
Philippines 45 2,616 323.345
Vietnam 1 219 .377
Subtotal 54 3,413 425.897
Sub-Sahara Africa (4)
Burundi 1 1 .099
Madagascar 1 410 82.000
Senegal 1 9 .018
Tanzania 1 26 2.100
Subtotal 4 446 84.217
West Africa (1)
Benin 3 126 2.740
Subtotal 3 126 2.740
West Asia & North Africa (3)
Egypt 1 3 .150
Iran 1 2 .340
Turkey 1 68 2.200
Subtotal 3 73 2.690
Grand total (21) 93 6,813 628.437
Annual Report 2013
Research support services 2013
Table 12. Origin and corresponding total number of shipments, total number of seedlots, and
total weight of incoming transgenic rice seeds/grains (January to December, 2013).
Region/country Total shipments (no.) Total seedlots (no.) Total weight (kg)
East Asia (2)
China 1 11 .120
Taiwan 1 24 .480
Subtotal 2 35 .600
Europe and Central Asia (1)
Germany 1 1 .030
Subtotal 1 1 .030
Southeast Asia (1)
Philippines 2 170 11.650
Subtotal 2 170 11.650
Grand total (4) 5 206 12.280
Table 13. Nature of incoming transgenic and nontransgenic shipments with corresponding total
number of shipments, total number of seedlots, and total weight (January to December 2013).
Nature of shipment/origin Total shipments (no.) Total seedlots (no.) Total weight (kg)
Rice seeds
East Asia 8 2,521 25.323
Europe and Central Asia 1 1 .074
Latn America 1 143 19.200
South Asia 3 16 5.350
Southeast Asia 34 2,342 256.945
Sub-Sahara Africa 3 420 82.117
West Africa 2 36 2.300
West Asia & North Africa 3 73 2.690
Subtotal 55 5,552 393.999
HRDC seeds
East Asia 2 2 5.500
South Asia 11 28 56.000
Southeast Asia 7 141 60.200
Subtotal 20 171 121.700
Transgenic rice seeds
East Asia 2 35 .600
Europe and Central Asia 1 1 .030
Subtotal 3 36 .630
24
Research support services 2013
Nature of shipment/origin Total shipments (no.) Total seedlots (no.) Total weight (kg)
Golden Rice seeds
Southeast Asia 2 170 11.650
Subtotal 2 170 11.650
Milled rice seeds
Europe and Central Asia 1 2 .425
Southeast Asia 6 400 73.100
Subtotal 7 402 73.525
Milled & dehulled rice seeds
Southeast Asia 1 210 4.100
Subtotal 1 210 4.100
Dehulled rice seeds
Europe and Central Asia 1 3 .021
Southeast Asia 1 219 .377
Sub-Sahara Africa 1 26 2.100
Subtotal 3 248 2.498
Polished rice seeds
Southeast Asia 2 66 30.170
Subtotal 2 66 30.170
Ground rice grains
Southeast Asia 1 24 .170
Subtotal 1 24 .170
Rice and dehulled rice seeds
East Asia 1 39 1.000
Subtotal 1 39 1.000
Rice four
Southeast Asia 2 11 .835
West Africa 1 90 .440
Subtotal 3 101 1.275
Grand total 98 7,019 640.717
Annual Report 2013
Research support services 2013
Table 14. Consignees and corresponding total number of shipments, total number of seedlots, and total weight of
incoming transgenic and nontransgenic rice seeds/ grains (January to December, 2013).
Organizatonal unit Total shipments (no) Total seedlots (no.) Total weight (kg)
Analytcal Service Laboratory (ASL)
Ground rice grains 1 24 .170
Polished rice seeds 1 4 .170
Rice four 1 4 .800
Rice seeds 1 9 2.100
Subtotal 4 41 3.240
C4 Rice Center
Transgenic seeds 1 24 .480
Subtotal 1 24 .480
Crop and Environmental Sciences Division
(CESD)
Dehulled rice seeds 1 219 .377
Rice seeds 8 558 103.474
Transgenic seeds 1 1 .030
Subtotal 10 778 103.881
Grain Quality and Nutriton Center (GQNC)
Milled rice seeds 3 230 3.425
Milled and dehulled rice seeds 1 210 4.100
Rice four 2 97 .475
Rice seeds 6 191 47.400
Subtotal 12 728 55.400
Plant Breeding, Genetcs, and Biotechnology
(PBGB)
Dehulled rice seeds 2 29 2.121
Golden Rice 2 170 11.650
HRDC seeds 20 171 121.700
Milled rice seeds 1 8 5.600
Polished rice seeds 1 62 30.000
Rice seeds 33 4,083 135.230
Rice and dehulled rice seeds 1 39 1.000
Transgenic seeds 1 11 .120
Subtotal 61 4,573 307.421
Social Sciences Division (SSD)
Milled rice seeds 3 164 64.500
Rice seeds 2 34 .500
Subtotal 5 198 65.000
26
Research support services 2013
Organizatonal unit Total shipments (no) Total seedlots (no.) Total weight (kg)
T.T. Chang Genetc Resources Center (TTC-GRC)
Rice seeds 5 677 105.295
Subtotal 5 677 105.295
Grand total 98 7,019 640.717
Table 15a. Results of visual inspecton conducted on incoming nontransgenic
rice seeds received by SHU for post-entry clearance (January to December,
2013).
Observatons No. of infested seedlots Percent (%)
Weed-contaminated seedlots 0 0
Insect-damaged 93 3.34
Seeds with soil 0 0
a Based on 2,784 seedlots visually inspected
Table 15b. General quality of incoming nontransgenic rice seeds received by
SHU for post-entry clearance.
General quality Number of seedlots Percent (%)
Category 1 604 21.70
Category 2 0 0.00
Category 3 1,973 70.87
Category 4 207 7.44
b Based on 2,784 seedlots visually inspected (seedlot x 100 / total)
Table 16. Seedborne pathogens detected in incoming rice seeds received by SHU for post-entry
clearance (January to December, 2013).
Pathogens Afected Seedlots (%) Detecton level (%) Mean value (%)
Trichoconis padwickii 98.55 1 – 78 27.28
Curvularia spp. 92.75 1 – 62 17.05
Sarocladium oryzae 15.22 1 – 4 1.38
Bipolaris oryzae 75.36 1 – 50 4.86
Phoma spp. 57.97 1 – 10 1.89
Fusarium moniliforme 18.84 1 – 4 1.23
Tilleta barclayana 32.61 1 – 100 7.51
Nigrospora spp. 21.74 1 – 16 2.17
Microdochium oryzae 4.35 1 – 2 1.17
Aphelenchoides besseyia 0.72 1 – 2 2.00
Pyricularia oryzae 1.45 1 – 2 1.50
a
Actual nematode count based on 200 seeds/seedlot (n=138)
Annual Report 2013
Research support services 2013
Table 17. Origin, nature of materials, total number of shipments, and total number of samples of incoming non-
transgenic and transgenic, nonseed biological materials (January to December, 2013).
Region/Country Total shipments (no.) Total samples (no.)
EAST ASIA (3)
Korea S
DNA rice 1 181
China
Primers 1 4
Taiwan
Antbody 1 1
Rice leaves 1 1050
Subtotal 4 1,236
EUROPE (6)
Belgium
DNA bacterial isolates 1 37
France
Sorghum seeds 1 16
Germany
Bacterial DNA, cultures Xoo & Xoc, PCR reagents,
phosphate bufered saline 1 126
DNA rice leaves 1 126
DNA plasmids 1 11
Plasmids 1 3
Netherlands
Grass, pepper (fruit & leaf), cherry laurel, and polished
rice ground 1 4
Plant samples (maize, staghorn, grass, potato) ground 1 4
Plant samples (tomato, grass, lucerna, oilpalm) ground 1 4
Plant samples (willow wood, summer barley,
milk thistle seed & grass) ground 1 4
Soil ground 3 12
Soil ground-dried 1 4
Switzerland
Tobacco seeds 1 1
United Kingdom
Antbody 3 40
Cleome seeds 1 2
DNA plasmids 3 6
DNA primers 1 4
Plasmids 5 17
Plasmids and primers 2 21
Subtotal 30 442
NORTH AMERICA (1)
USA
Antbody 1 2
28
Research support services 2013
Region/Country Total shipments (no.) Total samples (no.)
Plasmids 4 122
Setaria seeds 2 3
Subtotal 7 127
SOUTH ASIA (1)
Bangladesh
Soil 1 81
Subtotal 1 81
SOUTHEAST ASIA (4)
Indonesia
Rice straw 1 378
Philippines
Arthropods 1 290
Assorted vegetables 1 15
Bamboo plant parts ground 2 122
Buterfy 1 292
DNA rice 1 86
Fruits and vegetables 5 71
Herbarium and insect samples 1 9,578
Honeybee 1 4,602
Insects 2 2,994
Mungbean 1 24
Rice leaf and tllers fresh 1 240
Rice root & shoot samples 1 480
Rice straw transgenic and nontransgenic from Golden Rice 1 165
Rice straw, rice leaves, water 1
22
Snails, worms, diferent insects 1 117
Soil 5 46
Soil ground 2 185
Soil and straw ground 1 48
Sorghum and sugarcane leaves 1 2
Thailand
DNA bacterial isolates 1 114
Vietnam
DNA rice 1 27
Subtotal 33 19,898
SUB-SAHARA AFRICA (2)
Senegal
DNA rice 1 40
Tanzania
DNA rice 1 188
Subtotal 2 228
Grand total (17) 77 22,012
Annual Report 2013
Research support services 2013
Table 18. Consignees/recipients of incoming nontransgenic and transgenic, nonseed biological materials (January to
December, 2013).
Organizatonal unit/nature of materials Total shipments (no.) Total samples (no.)
Analytcal Service Laboratory (ASL)
Bamboo plant parts 2 122
Grass, pepper (fruit & leaf), Cherry Laurel, and polished rice 1 4
Plant samples (maize, staghorn, grass, potato) 1 4
Plant samples (tomato, grass, lucerna, oilpalm) 1 4
Plant samples (willow wood, summer barley, milk thistle seed
& grass) 1 4
Soil 6 201
Soil and straw 1 48
Subtotal 13 387
C4 Rice Center
Antbody 5 43
Cleome seeds 1 2
DNA plasmids 4 17
DNA primers 1 4
Plasmids 7 21
Plasmids and primers 2 21
Rice leaves 1 1,050
Setaria seeds 2 3
Subtotal 23 1,161
Crop and Environmental Sciences Division (CESD)
Arthropods 1 290
Buterfy 1 292
Herbarium and insect samples 1 9,578
Honeybee 1 4,602
Insects 2 2,994
Mungbean 1 24
Primers 1 4
Rice root & shoot samples 1 480
Rice straw 1 378
Rice straw, rice leaves, water 1 22
Snails, worms, diferent insects 1 117
Soil 6 127
Sorghum seeds 1 16
Subtotal 19 18,924
30
Research support services 2013
Organizatonal unit/nature of materials Total shipments (no.) Total samples (no.)
Plant Breeding, Genetcs, and Biotechnology (PBGB)
Assorted vegetables 1 15
Bacterial DNA, cultures Xoo & Xoc, PCR reagents, phosphate bufered
saline
1 126
DNA 8 799
Fruits and vegetables 5 71
Plasmids 3 121
Rice leaf and tllers 1 240
Rice straw 1 165
Sorghum and sugarcane leaves 1 2
Tobacco seeds 1 1
Subtotal 22 1,540
Grand total 77 22,012
Annual Report 2013
Research support services 2013
Table 19. Incidence (%) of diferent diseases observed during feld inspecton at three diferent crop stages of incoming and
outgoing rice materials in 2013.
Crop Stages/diseases Incoming Outgoing
Seedling DS %* WS %* DS %* WS %*
Total entries 5,495 344 24,526 17,472
Without disease 5,490 99.90 341 99.12 24,464 99.75 17,311 99.08
With disease 5 0.09 3 0.87 62 0.25 161 0.92
Schlerotum seedling blight 5 0.09 2 0.58 62 0.25 161 0.92
Bacterial brown stripe 0 0.00 1 0.29 0 0.00 0 0.00
Bakanae 0 0.00 0 0.00 0 0.00 0 0.00
Blast 0 0.00 0 0.00 0 0.00 0 0.00
Tillering
Total entries 5,495 344 24,526 17,472
Without disease 5,461 99.38 202 58.72 24,365 99.34 16,528 94.60
With disease 38 0.69 147 42.73 161 0.66 953 5.46
Rice tungro 38 0.69 141 40.99 156 0.64 213 1.22
Bacterial leaf streak 0 0.00 5 1.45 0 0.00 720 4.12
Yellow dwarf 0 0.00 0 0.00 0 0.00 1 0.01
Leaf scald 0 0.00 0 0.00 0 0.00 0 0.00
Bakanae 0 0.00 0 0.00 5 0.02 0 0.00
Sheath blight 0 0.00 0 0.00 0 0.00 0 0.00
Leaf bast 0 0.00 0 0.00 0 0.00 19 0.11
Bacterial leaf blight 0 0.00 1 0.29 0 0.00 0 0.00
Maturity
Total entries 5,495 344 24,526 17,472
Without disease 5,393 98.14 118 34.30 24,486 99.84 16,241 92.95
With disease 102 1.86 238 69.18 41 0.16 1409 8.05
Rice tungro 43 0.78 106 30.81 15 0.06 62 0.35
Bacterial leaf streak 0 0.00 108 31.40 0 0.00 825 4.72
Sheath blight 0 0.00 1 0.29 6 0.02 313 1.79
Leaf scald 0 0.00 4 1.16 0 0.00 1 0.01
Sheath rot 18 0.33 3 0.87 8 0.03 20 0.11
Narrow brown leaf spot 0 0.00 0 0.00 9 0.04 31 0.18
Yellow dwarf 0 0.00 0 0.00 0 0.00 0 0.00
Leaf blast 0 0.00 0 0.00 0 0.00 0 0.00
Neck blast 0 0.00 0 0.00 0 0.00 5 0.02
Bacterial leaf blight 0 0.00 0 0.00 0 0.00 0 0.00
False smut 41 0.75 16 4.65 3 0.01 152 0.87
Diseases observed on plants originatng from incoming seeds were not of an introduced nature.
* Disease incidence (%) is calculated as the number of plant units infected expressed as percentage of the total units assessed.
32
Research support services 2013
Table 20a. Diferent types of Material Transfer Agreements (MTA) for outgoing transgenic and nontransgenic rice
seeds/grains including INGER nursery sets (January to December, 2013).
Type of MTA Total MTAs issued (no.) Total shipments (no.) Total seedlots (no.) Total weight (kg)
Standard MTA
a
266 267 13,212 511.965
Standard MTA + OMTA 464 193 51,216 3,367.406
Signed SMTA + CMTA 22 13 1,742 15.900
HRDC MTEA 15 15 1,171 103.200
Amendment leter 4 4 389 .855
Transgenic MTA 4 4 63 .140
Collaboratve MTA 3 3 727 5.600
Restricted MTA 3 3 24 .300
Golden Rice MTA 3 3 11 .085
Non-IRRI Seed MTA 2 2 31 12.592
Transmital leter 2 2 23 .510
Signed SMTA + TEA 2 1 18 18.100
Restricted MTA and
Transgenic MTA 2 1 11 1.100
Signed SMTA 1 1 758 6.500
Leter of intent 1 1 1,351 23.000
Reference Laboratory
MTA
1 1 12 .095
NO MTA
b
0 73 28,793 770.711
TOTAL 795 587 99,552 4,838.059
a
Two or more Standard MTAs were issued in one shipment because of a large volume of ancestrals of lines under develop-
ment (SMTA-UD) generated from the system or there are diferent receiving insttutons/locaton sites in one seed request
applicaton.
b
No MTA issued for the materials originally came from the sending insttuton/country of destnaton (e.g., Korean seeds
multplied to IRRI, seeds for commercial analysis, IRRI to IRRI seed transfer hubs and seeds for IRRI experimental/training
purposes. A transmital leter provided to a shipment with the previously approved MTA sending the same materials and
an amendment leter for shipment using the previously approved MTA with additonal samples.
Table 20b. Diferent types of Material Transfer Agreements (MTA) with corresponding total number of shipments,
total number of seedlots, and total weight of incoming transgenic and nontransgenic rice seeds/grains (January to
December, 2013).
Type of MTA Total no. of shipments Total no. of seedlots Total weight (kg)
Standard MTA 28 1,536 81.106
NO MTAc 27 3,292 215.465
HRDC MTEA 16 34 69.500
Other MTA 12 857 41.981
Leter of intent 8 332 34.225
Signed SMTA 4 840 159.140
Collaboratve MTA 3 128 39.300
Total 98 7,019 640.717
c
No MTA accompanied by the seed package for the materials under Korean Seeds for Multplicaton Project (KSMP),
IRRI seeds sent back and seeds commercially obtained provided by invoice.
Annual Report 2013
Research support services 2013
Table 21. Volume of disposed transgenic materials from diferent organizatonal
units (January to December 2013).
Organizatonal unit/nature of materials
Quantty (/batches/plas-
tc bags/packets/pails)
C4 Rice Center
Autoclaved seeds 25
Seed 9
Soil 3
Vegetatve waste 59
Subtotal 96
Crop and Environmental Sciences Division (CESD)
Autoclaved seeds 11
Seeds 23
Soil 51
Vegetatve waste 25
Subtotal 110
Plant Breeding, Genetcs & Biotechnology (PBGB)
Autoclaved seeds 147
Seeds 26
Soil 4,172
Vegetatve waste 208
Subtotal 4,553
Grand total 4,759
34
Research support services 2013
Table 22. Volume of transferred transgenic materials conducted by diferent organizatonal units (Janu-
ary to December 2013).
Organizatonal unit/nature of materials
Quantty
(/batches/plastc bags/packets/
pails/no. of seedlings)
C4 Rice Center
Autoclaved seeds 6
Bags 2
Biomass 250
Crossed seeds 2
Germinated seeds 129
Panicles 3
Plants 6,259
Rice plants 116
Seedlings 1,737
Seeds 6,738
Seeds and plants 200
Seeds and vegetatve 130
Subtotal 15,572
Crop and Environmental Sciences Division (CESD)
Germinated seeds 27
Seedlings 128
Seeds 167
Subtotal 322
Plant Breeding, Genetcs, and Biotechnology (PBGB)
Germinated seeds 13
Panicles 212
Paper bag 2
Plants 1,461
Rachis seeds 95
Seedlings 1,201
Seeds 1,905
Seeds and panicles 36
Seeds and rachis 17
Subtotal 4,942
Grand total 20,836
Annual Report 2013
Research support services 2013
Analytcal Service
Laboratory (ASL)
ASL’s performance under full cost recovery
(FCR)
Efectve January 2013, ASL operated on FCR for all its
routne testngs and use of facilites. A review of the
year’s revenues show that ASL was able to recover
only 38% of its operatonal costs, short of its 50%
target for 2013. One reason for its low recovery was
that the projected number of sample submissions
and analysis requests for 2013 did not materialize
as IRRI customers opted to build their own capacity
to perform some of the routne testngs ofered by
ASL. Another reason was that other IRRI research
groups bought their own ovens and grinders instead
of rentng ASL facilites, thus reducing the need. Also,
ASL shifed its client focus to capture external market
for additonal revenue, while the Grain Quality and
Nutriton Center restructured its two service facilites
(ASL and QES) into an integrated service facility
(GQNSL). As part of restructuring, a core collecton
of research samples that ASL was involved in was
established. This includes work in the analysis and
data generaton of protein and micronutrients to
combine with traditonal grain quality traits for
building research concepts with genomics. These can
identfy the genes/pathways that determine grain
quality and nutriton and demonstrate to breeders
how breeding programs should be targeted. Around
3,000 GQ research samples were analyzed. The
revenue was only for the cost of consumables.
Analytical services
ASL completed the analysis of 79,581 assays for
18,237 samples, representng 98% of the total assay
requested and 91% of the total samples received
from various IRRI OUs (Fig.1). Plant and grain
samples consttuted the majority of the samples
analyzed (91%), soil was 5%, and soluton samples
were 4%.
Diferent OUs that availed ASL services came
from GQNC (52%), CESD (40%), PBGB (6%), and
external customers (1%) (Fig. 2). Kjeldahl N was the
most requested assay, followed by mult-element
analysis.
Status of its ISO 17025 Accreditaton
ASL maintains its compliance to the ISO 17025
standards and received its new PAO ISO 17025 Scope
of Accreditaton from the Philippine Accreditaton
Ofce (PAO) last 29 July 2013. It has added new tests
to its scope covering 97% of its routne analytcal
testng.
PAO also appreciates L. Molina’s contnuous
sharing of expertse and actve involvement in PAO
actvites as the technical assessor for laboratory
accreditaton and member of the Laboratory
Accreditaton Commitee.
Fig. 1. Completed number of samples and analyses per sample per sample type.
Soil Plant/Grain Soluton Total
1257
5443
15310
70891
# of samples
# of analyses
1670
3247
18237
79581
36
Research support services 2013
Radioisotope laboratory
Projects assisted through the use of the radioisotope
laboratory facilites and liaison services of the PNRI
were on rice transformaton using disease- and pest-
resistant genes and on greenhouse gas emission
measurement.
The annual leak test for IRRI’s GC-ECDs was
facilitated by the RSO to ensure the safe use
of sealed sources of radioactve materials for
greenhouse gas emission measurement.
PBGB
6%
ASL
1%
External
1%
CESD
40%
GQNC
52%
The RSO, in coordinaton with PBGB and GQNC
users, facilitated the applicaton of IRRI’s license from
the Center for Device Regulaton, Radiaton Health
and Research-Department of Health and the Food
and Drug Administraton to operate an X-ray facility
for two XRF units.
Staf training
L.Molina presented an oral paper at the Internatonal
Symposium in Soil and Plant Analysis in New Zealand
last April and partcipated in study tours on premier
commercial and government testng laboratories to
learn their best practces. She conducted a series of
echo-seminars and training actvites for ASL staf and
shared other valuable informaton to IRRI RMQA and
CPS teams.
Other technical training atended by E.J. Cruz, R.
Gonzales and R. Chavez were on hazardous materials
awareness, managing chemical risks in the lab, and
on developing your own chemical safety manual.
In-house training on quality evaluaton testng were
atended by J. Lapis and L. Molina. Some ASL staf
during its weekly meetngs partcipated in several
training actvites related to ISO 17025 standards .