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Intel ISEF 2010

International
Rules & Guidelines

International Rules for
Precollege Science Research:

Guidelines for
Science and Engineering Fairs

2009-2010

A Publication of
Society for Science & the Public
1719 N Street, NW
Washington, DC 20036-2888
Tel: 202/785-2255; Fax: 202/785-1243
email: sciedu@societyforscience.org
specific rules questions: src@societyforscience.org

Available online: http://www.societyforscience.org/isef/primer/rules.asp
Downloadable at: www.societyforscience.org/isef/document/index.asp

International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 Page 1

Table of Contents
Acknowledgments ........................................................................................................................................................... 3
ISEF SRC Members .......................................................................................................................................................... 3
Changes & Modifications for 2009-2010 .......................................................................................................................... 4
Intel ISEF Categories and Subcategories ......................................................................................................................... 5
Display and Safety Regulations
General Requirements ............................................................................................................................................... 6
Maximum Size of Project ........................................................................................................................................... 6
Items Required to be Visible at Project at the Intel ISEF ........................................................................................... 6
Items Required to be at Project But Not Displayed at the Intel ISEF ........................................................................ 6
Handouts and Official Abstract at the Intel ISEF ...................................................................................................... 6
Items Not Allowed at Project or in Booth ................................................................................................................. 7
Items Allowed at Project or in Booth BUT with the Restrictions Indicated .............................................................. 7
Electrical Regulations at the Intel ISEF ..................................................................................................................... 8
Other Intel ISEF Information and Requirements ....................................................................................................... 8
Intel ISEF Ethics Statement .............................................................................................................................................. 9
Intel ISEF Eligibility/Limitations ....................................................................................................................................... 9
Intel ISEF Requirements ................................................................................................................................................... 9
Continuation of Projects .................................................................................................................................................. 10
Team Projects ................................................................................................................................................................... 10
Roles and Responsibilities of Students and Adults
1) The Student Researcher(s) .................................................................................................................................. 11
2) The Adult Sponsor .............................................................................................................................................. 11
3) The Qualified Scientist ......................................................................................................................................... 11
4) The Designated Supervisor ................................................................................................................................. 11
5) The Institutional Review Board (IRB) .................................................................................................................. 11
6) The Affiliated Fair Scientific Review Committee (SRC) ........................................................................................ 12
7) Other Review Committees .................................................................................................................................... 12
8) The ISEF Scientific Review Committee ................................................................................................................ 12
Human Subjects
Rules ......................................................................................................................................................................... 13
Informed Consent ..................................................................................................................................................... 14
Risk Assessment ....................................................................................................................................................... 15
Sources of Information ............................................................................................................................................. 16
Vertebrate Animals
Rules for ALL Studies Involving Vertebrate Animals ............................................................................................... 17
Additional Rules for Projects Conducted in a Non-regulated Site ............................................................................ 18
Additional Rules for Projects Conducted in a Regulated Research Institution ........................................................ 18
Sources of Information ............................................................................................................................................. 19
Potentially Hazardous Biological Agents
Rules for ALL Studies Involving Potentially Hazardous Biological Agents ............................................................. 21
Additional Rules for Projects Involving Unknown Microorganisms ........................................................................ 22
Additional Rules for Projects Involving Recombinant DNA (rDNA) Technologies ................................................. 22
Additional Rules for Projects Involving Tissues and Body Fluids Including Blood and Blood Products ............... 22
Risk Assessment ....................................................................................................................................................... 23
Classification of Biological Agents Risk Groups and Levels of Biological Containment .......................................... 23
Sources of Information ............................................................................................................................................. 24
Hazardous Chemicals, Activities or Devices
Rules for ALL Studies Involving Hazardous Chemicals, Activities or Devices ........................................................ 25
Additional Rules for Projects Involving DEA-controlled Substances, Prescription Drugs,
Alcohol & Tobacco, Firearms & Explosives .................................................................................................... 25
Risk Assessment for Hazardous Chemicals, Devices and Radiation ........................................................................ 26
Sources of Information ............................................................................................................................................. 27
Forms
Information on Required Abstract ............................................................................................................................ 28
Forms ........................................................................................................................................................................ 29
Student Handbook (insert)

Page 2 International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010

Acknowledgments
Fair directors, teachers, scientists, parents, and adult volunteers inspire and encourage students to explore and
investigate their world through hands-on research. Those of you who work with these young people are rarely
recognized and never can be adequately thanked. Without you, precollege science and engineering projects and
science and engineering fairs would not be possible. We applaud your commitment and appreciate your hard work.
We sincerely hope that our efforts to enhance these Rules will serve you in working with students.
Please address any general questions regarding the Intel ISEF to:
Society for Science & the Public
Science Education Programs
1719 N Street, NW, Washington, DC 20036
office: 202/785-2255, fax: 202/785-1243, sciedu@societyforscience.org

For specific rules questions, please email:
SRC@societyforscience.org
The ISEF SRC members listed below will be using the above email address to respond to rules inquiries.

Intel ISEF SRC
Dr. Nancy Aiello, Chairperson (EST)
home: 540-554-8748
Dr. James Stevens (MST)
office: 303-724-0424, home: 303-696-1504, cell: 303-921-1076, fax: 303-724-3005
Mr. Henry Disston (EST)
office: 215-895-5840, fax: 215-895-5842
Mrs. Christine Miller (PST)
home: 775-847-7129, cell: 775-722-3134
Mrs. Evelyn Montalvo (EST)
(English or Spanish inquiries)
school: 787-834-2150, home: 787-833-0287, fax: 787-265-2500
Dr. Paula Johnson (PST)
office: 520-621-3483
Dr. Patricia Bossert (EST)
home: 631-757-5411; cell: 631-793-8237
Dr. Jennifer Green (EST)
office: 513-529-2448
Mr. Marcus Friskop (CST)
school: 701-242-7138; home: 701-242-7650
Mr. Jason Shuffitt (CST)
home: 270-843-6635; cell: 270-792-2557

These Rules apply to the
Intel International Science and Engineering Fair 2010
San Jose, California, USA, May 9-15, 2010
PERMISSION TO REPRINT WITH CREDIT GRANTED
Available on our website at www.societyforscience.org/isef/
This is the last printed version of the International Rules and Guidelines;
The Rules will continue to be updated annually and made available on the website.

International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 Page 3

This list was generated from the SRC reviews leading up to the Intel ISEF. • The Intel ISEF Rules Wizard . guidance on creating a photo consent is in the Display & Safety section of the rules. Vertebrate Animals • A veterinarian must be consulted in experiments involving prescription drugs and/or nutritional supplements in a non-regulated setting. additional resources are on the website.The full text of the International Rules and the forms both in html and in a downloadable format. mentors. • Student researcher/Qualified Scientist can develop their own informed consent form based on sample provided. these rules and guidelines for conducting research were developed to facilitate the following: • protect the rights and welfare of the student researcher and human subjects • protect the health and well-being of vertebrate animal subjects • follow federal regulations governing research • offer guidance to affiliated fairs • use safe laboratory practices • address environmental concerns The Rules on the Web www.asp The International Rules and Guidelines for Science Fairs is available on the Society for Science & the Public website in a number of formats to better aid all of those involved in the process: students. fair directors and local. • Clarification of waiver of informed consent/assent/parental permission by IRB. In addition to providing the rules of competition. • International Rules and Guidelines . Changes & Modifications for 2009-May 2010 Human Subjects • Reorganization of the human subjects rules. Read these to get pointers on what NOT to do. • Reorganization of Human Subjects Form 4.This “wizard” asks a series of questions about your planned project and will provide a list of forms that you need to complete. teachers. • Common SRC Problems .societyforscience. parents. • “Educator” replaces “science educator” for membership on IRB’s and SRC’s.org/isef/primer/rules. Potentially Hazardous Biological Agents • Commercially available coliform test kits require a Risk Assessment Form 3 • Laboratory studies utilizing MRSA and VRE are prohibited Form and Other Changes • Human Subjects Form 4 is no longer to be used as a photo consent. Form 4 no longer serves as an informed consent document. Page 4 International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 . regional and state scientific review committees (SRC) and institutional review boards (IRB).

Masers CHEMISTRY Other Theoretical Physics. Controls Epidemiology Clinical & Developmental Psychology Mechanical Engineering. Computer Soil Contamination and Soil Quality Plant Systematics. Forestry Development Recycling.. Theoretical or Analytical Chemistry Computational Astronomy General Chemistry ENVIRONMENTAL MANAGEMENT Other Inorganic Chemistry Bioremediation Organic Chemistry Ecosystems Management PLANT SCIENCES Physical Chemistry Environmental Engineering Agriculture/Agronomy Other Land Resource Management. dependent on the needs of their area. Bacteriology Metabolism Chemical Engineering Microbial Genetics Structural Biochemistry Industrial Engineering. Waste Management Ecology COMPUTER SCIENCE Other Genetics Algorithms. Lasers. Local. Astronomy Cellular and Molecular Genetics Aerodynamics Biological Physics Immunology Alternative Fuels Instrumentation and Electronics Molecular Biology Fossil Fuel Energy Magnetics and Electromagnetics Other Vehicle Development Nuclear and Particle Physics Renewable Energies Optics. Please visit our website at www. Solar Physiology and Pathophysiology Sociology Other Other Other ENGINEERING: Materials & Bioengineering MICROBIOLOGY BIOCHEMISTRY Bioengineering Antibiotics. Construction Eng.societyforscience. Mineralogy Analysis Animal Husbandry Paleontology Applied Mathematics Pathology Geophysics Geometry Physiology Planetary Science Probability and Statistics Populations Genetics Tectonics Other Systematics Other Other MEDICINE & HEALTH SCIENCES ENGINEERING: Electrical & Mechanical Disease Diagnosis and Treatment BEHAVIORAL & SOCIAL SCIENCES Electrical Eng. state and country fairs may or may not choose to use these categories. Molecules.org/isef/students/research_categories. Computer Eng. Cellular. Solids Cellular Biology Aerospace and Aeronautical Engineering. Operating System Other International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 Page 5 . Genetics Cognitive Psychology Robotics Molecular Biology of Diseases Physiological Psychology Thermodynamics.asp for a full description and definition of the Intel ISEF categories: ANIMAL SCIENCES EARTH & PLANETARY SCIENCE MATHEMATICAL SCIENCES Development Climatology.. Evolution Graphics Water Pollution and Water Quality Other Software Engineering.. Please check with your affiliated fair(s) for the appropriate category listings at that level of competition. regional. Data Bases Photosynthesis Artificial Intelligence ENVIRONMENTAL SCIENCES Plant Physiology (Molecular. Weather Algebra Ecology Geochemistry. Processing Virology Other Material Science Other Other CELLULAR AND MOLECULAR PHYSICS AND ASTRONOMY BIOLOGY ENERGY & TRANSPORTATION Atoms. Programming Other Languages Computer System. Intel ISEF Categories and Subcategories The categories have been established with the goal of better aligning judges and student projects for the judging at the Intel ISEF. Antimicrobials General Biochemistry Civil Engineering. Networking and Communications Air Pollution and Air Quality Organismal) Computational Science.

The Intel ISEF Scientific Review Committee defines the “official abstract and certification” as an UNALTERED original abstract and certification as stamped/embossed by the Intel ISEF Scientific Review Committee. If the Scientific Review Committee requires a Finalist to make Curtained Back changes to the abstract and certification submitted with of Booth registration papers. Intel ISEF Display and Safety Regulations Please address any questions regarding Intel ISEF Display and Safety Regulations to: John O. and demonstrations for public and judges. and Certification at the Intel ISEF positioned at. will replace the earlier version. Handouts to judges and to the public must be limited to UNALTERED photocopies of the official abstract and certification. Student Checklist (1A). Page 6 International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 . All forms required for Scientific Review Committee supports. These forms and any informed consents forms should not be displayed. not exceed allowed dimensions. any project with a component that will Intel ISEF officials. SRC/DS2 (Received on-site at the Fair) Maximum Size of Project • Regulated Research Institutional/Industrial Setting Form (1C) — when applicable Depth (front to back): 30 inches or 76 centimeters • Continuation Projects Form (7) — when applicable Width (side to side): 48 inches or 122 centimeters • Photograph / image credits Height (floor to top): 108 inches or 274 centimeters At the Intel ISEF. the component plus the project must (other than the Finalist) displayed as part of the project. In addition. Cole. the Display & Safety be demonstrated by the Finalist must be demonstrated Committee requires a photograph/video release form only within the confines of the Finalist’s booth. itself exceed the allowed dimensions nor may the table Approval Form (1B). but not limited to Checklist for Adult table is used. it becomes part of the project and must not Sponsor (1). the revised version will be stamped/ embossed. Displayed at the Intel ISEF Maximum project sizes include all project materials. the Intel ISEF Display and Safety Scientific Review Committee Committee may require students to make revisions in • Completed Intel ISEF Project Set-up Approval Form their display to conform to display and safety regulations. and will become the Finalist’s official abstract and certification. Display and Safety Committee Chair. fair-provided tables will not exceed a Required to Be at the Project But Not height of 36 inches (91 centimeters). When not signed by the human subject for visual images of humans being demonstrated. approved and stamped/embossed by the Intel ISEF Occasionally. do not plus any part of the project exceed the allowed have to be displayed as part of the project but must be dimensions. Position of Project Handouts/Official Abstract and Table or freestanding display must be parallel to. the back curtain of the booth. E-mail: dejavu60@msn.com General Requirements Required to Be Visible and Vertically The Intel ISEF Display and Safety Committee is the final Displayed at the Intel ISEF authority on display and safety issues for projects • Original of official Abstract and Certification as approved by the SRC to compete in the Intel ISEF. An original stamped/embossed official abstract and certification must appear on the display board or in a vertical position at the project. The only abstract allowed anywhere at a project is the official abstract. If a approval including. Research Plan. The term “abstract” may not be used as a title or reference for any information on a Finalist’s display or in a Finalist’s materials at the project except as part of displaying the official abstract. and Human Subjects (4). available in the booth in case asked for by a judge or other At the Intel ISEF.

weapons. syringes. performed within the maximum size of the project permitted. medals. sealed apparatus. Any apparatus producing temperatures that will cause previously contained combustible liquids or gases. 14.. Glass or glass objects unless deemed by the Display and e. newspapers. ammunition. Soil. Awards. and/or waste samples if permanently encased in a slab of acrylic 3.) one of the groups mentioned above is final. visually offensive project or display) photographs or visual depictions of invertebrate or 7. Activities. The only items that may be displayed on the front of the provided tables are the forms listed in the section of these rules entitled “Required to be Visible and Vertically Displayed at the Intel ISEF” International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 Page 7 . endorsements. physical burns if adequately insulated pressurized tanks. empty tanks that 8.) and not operated. firearms. They pose a risk if the beam is directly viewed over a long period of time. including humans.) 9. Any demonstration for judges or the public must be vertebrate animals in surgical techniques. They are photographs of human subjects for which signed 13.) 11. This manufactured construction materials used in building the includes. reloading devices. consent forms are at the project or in the booth. and/or acknowledgments (graphic or 4. needles.) 9. commercial product such as a computer screen) 7. or Society for Science & the Public. Living organisms. dissections. rock. “Year Two of an Look into Beam” Ongoing Study”)]. Labeled with a sign reading “Laser Radiation: Do Not which year the project is (for example. etc. Dry ice or other sublimating solids c. 12. Class II lasers if: or operating the project at the Intel ISEF a. Plant materials (living. one credit knives) prominently and vertically displayed is sufficient. poisons. Preserved vertebrate or invertebrate animals 2. flags.” drugs. 8. b. Photographs and/or visual depictions if: urine) a. Batteries with open-top cells e.. Sharp items (for example. one credit line prominently and vertically displayed Restrictions Indicated”)] is sufficient. (If all 10. Any apparatus with unshielded belts. including plants the Restrictions Indicated 2. magazines. dead. Any apparatus deemed unsafe by the Scientific Review (See the description of Class III and Class IV lasers in the Committee. photographs/images are from the same source. Flames or highly flammable materials d. or non-manufactured state (Exception: Committee. Class III and IV lasers if for display only and not operated 18. business cards. or written) unless the item(s) are an integral part of the project moving parts with tension or pinch points if for display only (Exception: Intel ISEF medal(s) may be worn at all times. Active Internet or e-mail connections as part of displaying 6. unprocessed.Not Allowed at Project or in Booth Allowed at Project or in Booth BUT with 1. pipettes.. the Display and Safety raw. or for Science & the Public (for example. Human or animal food telephone and fax numbers of Finalist only 5. p. etc. the Display and Safety Committee. They are photographs or visual depictions of the Finalist. necropsies. dangerous ray-generating devices. or Society Radiation section of the Hazardous Chemicals. and credit lines are attached. All hazardous substances or devices [for example. 27. Photographs or other visual presentations depicting 5. Disconnected when not operating Safety Committee to be an integral and necessary part of Note: Class II lasers are found in laser pointers and in the project (Exception: glass that is an integral part of a aiming and range-finding devices. The decision by any an enclosed. blood.. and visual access to beam 17. The output energy is <1 mW and is operated only by the 16. Operated only during the Display and Safety inspection vertical display board. pulleys. Enclosed in protective housing that prevents physical Continuation Project Form (7) vertically displayed.”). or other lab procedures an area 30”(Depth) by 48”(Width) by 108” (Height) 15. chains. They are from the Internet. or preserved) that are in their Scientific Review Committee. logos. Postal addresses. but is not limited to. 3. They have credit lines of origin (“Photograph taken by. World Wide Web and e-mail addresses. or “Image taken from.. (If all photographs being and lasers (as indicated in item 5 in the section of these displayed were taken by the Finalist or are from the same rules entitled “Allowed at Project or in Booth BUT with the source. Prior years’ written material or visual depictions on the Finalist b. journals. large vacuum tubes or Devices. Taxidermy specimens or parts 1. They are not deemed offensive or inappropriate by the 6. Continuation projects must have the d. Human/animal parts or body fluids (for example. sand. [Exception: the project title and during judging displayed in the Finalist’s booth may mention years or c. All chemicals including water (Exceptions: water integral to vertebrate animals. 4.

” or “220 Volt A. 5. Finalists using audio-visual or multi-media presentations other than the Finalist. except small containers of bottled water for personal consumption. graphics. with UL-listed connectors. Society for Science & the Public. The inspection is a process that 2. Society for Science & the Public will remove the source and prior to any project equipment. switches. Project sounds. Maximum circuit amperage/wattage Finalist except for an interpreter if necessary. or any other display items must not be distracting. CDs.” devices (such as fuses) and must be inaccessible to anyone 6. etc.. Electrical power supplied to projects and. No food or drinks. the entire presentation to the Display and Safety inspectors 5. If a project fails to qualify and is not removed by the fuse or circuit breaker on the supply side of the power Finalist. images. therefore. Page 8 International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 . halogen lights.C. Finalists must be present at their projects for the Display and Safety inspection. are highly recommended. and if under 18 fuses. the takes place between the Finalist and inspector. Any disks.” is intended to encompass the and the Scientific Review Committee. switches. Wiring. therefore. and other rules may apply to specific configurations. 1. also by the guardian of the subject. For all electrical regulations. All electrical work must conform to the National Electrical protect the integrity of the Intel ISEF and its rules and Code or exhibit hall regulations. etc. electrical circuits must Requirements provide a UL-listed 3-wire extension cord which is appropriate for the load and equipment. etc. etc. must be turned off when the Finalist is not present. extension cords.. here are general ones. have a photo release signed by the subject. A. or other conducting material or with a grounded metal box to prevent non-print presentation methods) must be prepared to show accidental contact. No changes. odors.) involving my participation/my child’s must have adequate insulation and over-current safety participation in this research. project in the safest manner possible but is not responsible 6. printed materials. videotapes. 60 cycle. maximums allowed for projects is 120 or 220 Volt. lights. Exposed electrical equipment or metal (for example. 9. are allowed in the Exhibit Hall. All electrical connectors. The guidelines presented regulations. 10. must be UL-listed and must be appropriate for the years of age. (including unofficial source. “120 made after approval by the Display and Safety Committee Volt A. and metal parts videos. The on-site electrician may review electrical 4. Sample load and equipment.C. that possibly may be energized must be shielded with a non- animations. Electrical Regulations at the Intel ISEF Other Intel ISEF Information and 1. available is determined by the electrical circuit capacities of the exhibit hall and may be adjusted on-site by the Display 2. modifications.) will be discarded immediately. displayed on computer monitors.C. other means of disconnect from the 120 or 220 Volt power 8. clearly visible on/off switch or for damage to the project. no other persons should be present representing the single phase. corresponding range of voltage as supplied by the facility in 3. abstracts) designed to be distributed to judges or the public 7. A project data book and research paper are not required but work on any project. Any lighting that generates considerable and excessive will be confiscated by the Display and Safety Committee and amounts of heat (high-intensity lamps. Finalists requiring 120 or 220 Volt A.C. appliance or piece of equipment must have a clearly visible 7. Wiring not part of a commercially available UL-listed before the project is approved. There must be an accessible. Display of photographs other than that of the finalist must 4. the Scientific Review which the Intel ISEF is being held. Committee. 35mm slides. and/or the Display and Safety Committee reserve the right to remove any project for safety reasons or to 3. Connections must be soldered or made consent text: “I consent to the use of visual images (photos. wiring. or additions to projects may be and Safety Committee. etc.

ALL PROJECTS Ethics Statement 5) Intel ISEF exhibits must adhere to Intel ISEF display and safety requirements. state. abstract must describe research conducted by the 2) All projects must adhere to the Ethics Statement student. reapproved prior to experimentation/data collection for 6) Projects that are demonstrations. requirements). (See 7) A research project may be a part of a larger study Continuation Projects Form (7)) done by professional scientists. 2010. home. country and U. or informational projects. two or three Finalists to the Intel ISEF. International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 Page 9 . (See Student Federal laws. ‘explanation’ models or kit 12) Any continuing project must document that the building are not appropriate for the Intel ISEF. toxic chemicals or foreign substances into 16) All signed forms.pdf. ‘library’ research the current year. above. an Institutional Review or the Intel ISEF. Board (IRB) or Scientific Review Committee (SRC) must review and approve most projects involving Eligibility/Limitations human subjects. any 4) Students may compete in only one ISEF Affiliated proposed changes in the Student Checklist (1A) and Fair. required. but are recommended. See the appropriate Individual Project Finalists and one Team Project of sections of the Rules Book. one-page abstract an ISEF-affiliated fair must adhere to all of the rules which summarizes the current year’s work. Such sponsor to check with their affiliated fair for any practices include plagiarism. but the project 13) If work was conducted in a regulated research presented by the student may only be their portion of institution. and permits must be the environment is prohibited. preceding the Intel ISEF. 11) Projects which are continuations of previous year’s 5) Team projects may have a maximum of three work and which require IRB/SRC approval must be members. Regional fairs may have different 4) Introduction or disposal of non-native species. 15) A project data book and research paper are not 3) Projects must adhere to local. each student or team must 1) All domestic and international students competing in submit a (maximum) 250-word. DEA-controlled substances. Scientific fraud and misconduct are not condoned 6) It is the responsibility of the student and adult at any level of research or competition. except when proceeding to a state/national fair Research Plan must be re-approved before affiliated with the Intel ISEF from an affiliated laboratory experimentation/data collection resumes. school or field at any time during the current ISEF project year. many human continuous months between January 2009 and May subject studies and many vertebrate animal studies. use or additional restrictions or requirements. additional research is new and different. industrial setting or any work site other than the complete study. none of and review the project with the Adult Sponsor as the whom has reached age 21 on or before May 1 Checklist for Adult Sponsor (1) is completed. and potentially 1) Each ISEF-affiliated fair may send up to two hazardous biological agents. 9) A Qualified Scientist is required for all studies 3) Each student may enter only one project which involving BSL-2 potentially hazardous biological covers research done over a maximum of 12 agents.S. 8) Every student must complete Student Checklist 2) Any student selected by an ISEF-affiliated fair must (1A). vertebrate animals. See available for review by an SRC just before each fair a www. pathogens.gov/documents/isef. a Research Plan and Approval Form (1B) be in grades 9-12 or equivalent to be eligible. presentation of other researcher’s work as one’s own and fabrication of data. regulations and permitting conditions. regional fair. certifications. General 14) After experimentation. Handbook. forgery. The as set forth in this document. 10) After initial IRB/SRC approval (if required).anstaskforce. Fraudulent projects Approval and Documentation will fail to qualify for competition in affiliated fairs 7) Before experimentation begins. student enters. Regulated Research Requirements Institutional/Industrial Setting Form (1C) must be completed. not by adult supervisors.

but may be altered in investigation. NOTE: For competition in the Intel ISEF. then it is a continuation project for competition. return of flora and 6) Full names of all team members must appear on the fauna in a burned area over time. NOTE: could be considered a continuation project. each member of the team should be able 3) Display boards must reflect the current year’s work to serve as spokesperson. However. 4) Longitudinal studies are permitted as an acceptable 5) Each team member must submit an Approval Form continuation under the following conditions: (1B). No raw data from previous years may be displayed. a Research Plan and critical variable. etc.May 2010. The project year includes research project. documenting the same variables in which time is a a Student Checklist (1A). 2) Any project based on the student’s prior research 2) Teams may have up to three members.g. coordinate the work and act as spokesperson. research. drought on soil in a given basin. have Team Projects. the previous year’s abstract and research plan and the abstract for all other prior years. However. research projects may 1) Team Projects compete in a separate “team” be done that build on work done in previous years. The final work should reflect the coordinated Ongoing Study”). The study is a multi-year study testing or the Checklist for Adult Sponsor (1). Supporting data books (not efforts of all team members and will be evaluated research papers) from previous related research may using similar rules and judging criteria as individual be exhibited on the table properly labeled as such. Each consecutive year must demonstrate time- based change. The documentation should be clearly labeled in the upper right hand corner with the year (ex: 2008-2009). b. (Examples: Effect of high rain or other required forms. experimentation with the exact same methodology and research question or increasing sample size are 4) Each team should appoint a team leader to examples of unacceptable continuations. Page 10 International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 . be fully involved with the only. The display board must be based on collective past conclusionary data and its comparison to the current year data set. one abstract. If the Teams may not have more than three members at a current year’s project could not have been done local fair and then eliminate members to qualify for without what was learned from the past year’s the Intel ISEF. in addition to two individual projects.) Repetition of previous subsequent years. Please retain all prior years’ paperwork in case an SRC requests additional documentation. c. Continuation of Projects Team Projects 1) As in the professional world. These projects must document that the 3) Team membership cannot be changed during a given additional research is an expansion from prior work research year including converting from an individual (e. testing a new variable or new line of project or vice versa. category against all other Team Projects. documentation must include the Continuation Project Form (7). to the conducted over a maximum of 12 continuous months Intel ISEF.) abstract and forms. The project title displayed in the Finalist’s booth project. projects. ISEF-Affiliated Fairs are not required to from January 2009. An ISEF Students will be judged only on the most recent Affiliated Fair has the option of sending a team year’s research. and be familiar with all aspects of the may mention years (for example. team members must jointly submit a. but are encouraged to do so. “Year Two of an project.

licensed clinical professional counselor. b) that be reviewed and approved by an IRB before experimentation forms are completed by other adults involved in approving or begins. and c) that criteria for the to be used in a project. 4) The Designated Supervisor Scientific fraud and misconduct are not condoned at any level of research or competition. IRB. physician’s assistant. etc. A copy of the correspondence (e. However. etc. The Adult Sponsor is responsible for working with the student to 5) The Institutional Review Board (IRB) evaluate any possible risks involved in order to ensure the health and safety of the student conducting the research and the An Institutional Review Board (IRB) is a committee that. must evaluate the must review the student’s Student Checklist (1A) and Research potential physical and/or psychological risk of research Plan to make sure that: a) experimentation is done within local.) should be attached to Form 4 and can be used as relates to the student’s area of research. psychological risk involved in a given study: a medical The Adult Sponsor is responsible for ensuring the student’s doctor. vertebrate animals. and federal laws and these International Rules. Such practices include plagiarism. licensed social worker or research is eligible for entry in the Intel ISEF. The issues must be discussed with the student when a) an educator completing the Research Plan. the Designated Supervisor must be must have a solid background in science and should have close knowledgeable about the humane care and handling of the contact with the student during the course of the project. a psychiatrist. This includes review of any surveys or questionnaires supervising any part of the experiment. engineering. This individual influenced by humans. Some experiments involve b) a school administrator (preferably. All proposed human research must state. should be thoroughly familiar with the student’s project. degree with equivalent experience and/or expertise in the In order to eliminate conflict of interest.). the Qualified Scientist. following the the student will use. but qualify for competition in affiliated fairs or the Intel ISEF. laws. psychologist. the student must work locally with a Designated supervisory adults (Adult Sponsor. etc.g. and doing the experimentation. These may include chemical and equipment ISEF Affiliated Fair level must consist of a minimum of three usage. research involving human or members. Additional members are regulations that govern the student’s area of research. In research project including enlisting the aid of any needed this case. and the Designated Scientific Review Committee (SRC). The Adult Sponsor must be familiar with the regulations that therefore an IRB should be established at the school level to govern potentially dangerous research as they apply to a specific evaluate human research projects. Fraudulent projects will fail to Designated Supervisor need not have an advanced degree. and federal the IRB reviewing that project. if that person is qualified as outlined above. The Qualified Scientist must Supervisor who oversee a specific project must not serve on be thoroughly familiar with the local. student’s area of research is acceptable when approved by a parents. Qualified Scientist adhere to those set forth below. The Adult Sponsor may act as the Designated Supervisor. university professor. The Adult Sponsor according to federal regulations (45-CFR-46). email. recommended to help avoid this conflict of interest and to increase the expertise of the committee. a master’s the signature of that expert. professional degree in the biological or medical sciences as it fax. involved in the project. Rules & Guidelines of the ISEF. etc. state. then documented contact with an external expert is appropriate A Qualified Scientist should possess an earned doctoral/ and encouraged. registered nurse. and cell cultures. use or presentation of other researcher’s work as one’s responsible for overseeing student experimentation. An IRB at the school or student project. A student The student researcher is responsible for all aspects of the may work with a Qualified Scientist in another city or state.). Federal regulations require local community involvement. data analysis. Roles and Responsibilities of Students & Adults 1) The Student Researcher(s) The Qualified Scientist and the Adult Sponsor may be the same person. If not thoroughly familiar with the regulations. The own and fabrication of data. humans or animals involved in the study. the Adult c) and one of the following who is knowledgeable and Sponsor should help the student enlist the aid of a Qualified capable of evaluating the physical and/or Scientist. microorganisms. involving human subjects. animals are in a situation where their behavior or habitat is or scientist in whose lab the student is working. International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 Page 11 . The Designated Supervisor is an adult who is directly forgery. and must be trained in the student’s area of research. the Adult Sponsor. parent. a principal or vice procedures or materials that are regulated by state and federal principal). experimental techniques. or animal An IRB must include: tissues. Additional Expertise: If the IRB needs an expert as one of 3) The Qualified Scientist its members and one is not in the immediate area. Qualified Scientist. 2) The Adult Sponsor If a student is experimenting with live vertebrates and the An Adult Sponsor may be a teacher. Supervisor (see below) who has been trained in the techniques obtaining necessary approvals (SRC. animals.

proper institutional board before experimentation must also be Student Checklist (1A). Abstract. The operation and composition of the local c) Institutional Biosafety Committee (IBC) and ISEF-Affiliated Fair SRCs must fully comply with the d) Embryonic Stem Cell Research Oversight Committee International Rules. The ISEF SRC reviews the Checklist for Adult Sponsor (1). clarification). universities. D.) A fair director or ISEF Affiliated Fair SRC member with any b) an educator questions regarding the process. These certifications. Research Plan and Approval Form (1B) reviewed by the Fair SRC for rules compliance. M. They also identify problems local fairs may be having must include: and work with fair directors and teachers to resolve them. then measure is prescribed (e.) and which were reviewed and approved by the group of adults knowledgeable about research regulations. f) humane treatment of animals g) compliance with rules and laws governing human. The ALL projects must be reviewed and approved by the SRC after ISEF SRC reviews the forms and the research plan for all projects experimentation and shortly before competition in an ISEF. in reviewing projects just prior to a fair. the SRC may override the IRB’s j) compliance with the ISEF ethics statement decision and the project may fail to qualify for competition. etc. In some cases.D. If the SRC needs an expert as their procedures and research to the ISEF SRC. correctional facilities). the Qualified Scientist. the Adult Sponsor. (See page 3.. a simple corrective one of its members and one is not in the immediate area.. if an SRC and those involving potentially hazardous biological agents judges an IRB’s decision as inappropriate. affiliated Fair competition.V. committees that have been established at that institution.. Do parents. to ensure that students have followed all applicable Rules. Usually. D.g. Local SRCs may be a) Institutional Animal Care and Use Committee (IACUC) formed to assist the ISEF Affiliated Fair SRC in reviewing and b) Institutional Review Board (IRB) approving projects.IRBs exist at federally regulated institutions (e.. or rewriting an abstract for purposes of encouraged. animal research However.) If animal compete in the Intel ISEF. research plans and exhibits for compliance with the committees include: Rules and pertinent laws and regulations. at least one member must be familiar with questions about particular projects. e) evidence of search for alternatives to animal use An IRB generally makes the final determination of risk. The SRC Intel ISEF.. (Human 8) The ISEF Scientific Review Committee studies reviewed and approved by a properly constituted IRB do (ISEF SRC) not have to be reviewed by the SRC until the Fair competition. 6) The Affiliated Fair Scientific Review 7) Other Review Committees Committee Certain areas of research conducted in a regulated research A Scientific Review Committee (SRC) is a group of qualified institution require review and approval by federally mandated individuals that is responsible for evaluation of student research. Additional members are recommended to help avoid this conflict of interest and to increase the expertise of the committee.D. NIH. the ISEF SRC may have research is involved.g.S. signatures and dates showing maximum of one sponsored by that institution. The institutional IRB must initially b) evidence of proper supervision review and approve all proposed research conducted at or c) use of accepted and appropriate research techniques d) completed forms. or D. contacting the Designated Supervisor documented contact with an external expert is appropriate and to confirm a detail. should contact the Society for c) at least one other member Science & the Public or a member of the ISEF SRC. must not serve on the SRC reviewing that project. is made up of a high school. extended The ISEF SRC is the final authority on projects that are qualified to knowledge of biosafety or of human risk groups. Prisoner following: advocates must be included on the IRB when research subjects a) evidence of literature search are at a correctional facility. A Scientific Review Committee (SRC) examines projects for the medical centers. after students explain proper animal care procedures.M. Page 12 International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 . (ESCRO) Any proposed research in the following areas must be reviewed and approved BEFORE experimentation: projects involving vertebrates and potentially hazardous biological agents. a) a biomedical scientist (Ph.) Additional Expertise: Many projects will require additional expertise to properly evaluate (for instance..O. In order to eliminate conflict of interest. thereby placing i) documentation of substantial expansion for continuation projects human subjects in jeopardy. and the Designated Supervisor not send original forms to the Society for Science & the Public.) A Scientific Review Committee exists at the Intel ISEF level.) in addition to all other required forms for students who enter the An SRC must consist of a minimum of three persons.D. (Projects requiring preapproval which were conducted at a regulated research institution (not home or The ISEF SRC. like an ISEF Affiliated Fair SRC. It is important that students retain all original signed forms. The Adult Sponsor and the local year duration of research and appropriate preapproval dates IRB are responsible for ensuring that the project is appropriate (when needed) for a pre-college student and adheres to the ISEF rules.

. conduct research with human subjects. public park) in which all of the de-identified/anonymous (e. identified by review of the written documentation After initial IRB approval. baseball statistics. concept. Research subjects under 18 years of age or individuals not able to give consent (e.g. with their parents/guardians International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 Page 13 . include: approval or additional human subjects forms. phone number or other identifying a) the researcher has no interaction with the individuals variables. the Institutional Review Board (IRB) before the student may SRC must ensure that the data were appropriately de.g. sets that are publicly available or published and do not b)that occur in non-public or restricted access settings involve any interaction with human subjects or the (e. birth date. identifiable data. a student with any proposed provided by the supervising professional. begin recruiting and/or interacting with human subjects. Most human subject the definition of a human subject is a living individual about studies require preapproval from an Institutional Review Board whom an investigator conducting research obtains (1) data (IRB) and informed consent/assent from the research subject. mentally disabled) give their assent.g.. Adult research subjects give their consent. doctor’s office) collection of any data from a human subject for the purpose c) that involve the recording of personally identifiable of the student’s research project. tests) product. must be reviewed and approved by an b) during the final SRC review and approval process. day care setting.g. or (2) identifiable private information. 5) The research subjects must voluntarily give informed consent/assent (in some cases with parental permission) before participating in the study.) writing that the data have been appropriately de- identified and are in compliance with all privacy and 4) All research projects involving human subjects. Examples of • Subjects participating in physical activities (e. • Psychological..g. See risk assessment below and the online Risk Assessment • Projects in which the student receives the data in a de.. or samples through intervention or interaction with individual(s).) being observed 2) Student researchers must complete ALL elements of the b) the researcher does not manipulate the environment in Human Subjects portion of the Research Plan Instructions any way and on p.g. identified/anonymous format which complies with both 3) The research study should be in compliance with all conditions below: privacy and HIPAA laws when they apply to the project a) the professional providing the data must certify in (e. Guide for additional guidance. c) the researcher does not record any personally psychological and privacy risks to their human subjects. When students under the conditions and rules in the following sections. Examples of studies that are considered “human subjects research” requiring IRB preapproval Some studies involving humans are exempt from IRB pre. where personal data is not collected and where the • Studies in which the researcher is the subject of the testing does not pose a health hazard. where the feedback received is a direct reference to the surveys. It is recommended research that Risk Assessment Form (3) be completed. place an object).. physical exempt projects for ISEF and affiliated fairs include the exertion. These Exempt Studies projects require IRB review and preapproval and may also (Do Not Require IRB Preapproval or Human require documentation of written informed consent/assent/ Subjects Paperwork) parental permission.g. #1 and evaluate and minimize the physical. questionnaires. • Behavioral observations a) that involve any interaction with the observed • Data/record review studies (e. including HIPAA laws and any revisions. post a sign. information • Behavioral observations of unrestricted.g. of the participants must be protected. data set that includes following apply: name. changes in the Research Plan of the project must repeat the approval process before laboratory experimentation/data collection resumes. the rights and welfare Based upon the Code of Federal Regulations (45 CFR 46). shopping mall. any medical following: procedure) • Testing of a student designed invention. educational and opinion studies (e. 31.. program. public settings • Data/record review projects that include data that are not (e. crime individual(s) or where the researcher has modified the statistics) in which the data are taken from preexisting data environment (e. ingestion of any substance. Human Subjects The following rules were developed to help pre-college Rules student researchers follow federal guidelines (Code of Federal Regulations 45 CFR 46) designed to protect the human 1) The use of human subjects in science projects is allowable research subjects and the student researcher.g. the project involves medical information.. etc.

The IRB will determine whether 11) After experimentation and shortly before fair the consent/assent/parental permission may be verbal or competition. scored and interpreted by a Qualified consent/assent/parental permission be obtained. or game theory and do NOT involve the direct supervision of a qualified professional. b) obtaining informed consent and c) ensuring that participants are of the appropriate age to give informed consent. A copy of the IRB b) Research on individual or group behavior or approval for the entire project (which must include the characteristics of individuals where the research procedures/measures the student is using) or an researcher does not manipulate the subjects’ official letter from the IRB attesting to this approval is behavior and the study does not involve more required. or activities that are 7) A student may observe and collect data for analysis of determined by the IRB to involve perception. documentation of IRB review and approval. Students are prohibited from d) Studies involving physical activity where the administering medications and performing invasive medical IRB determines that no more than minimal risk procedures on human subjects. parents or guardians) about the risks and e. (Public Health Service appropriateness of waiving written informed consent/ Act. 10) Studies that involve the collection of data via use of the internet (e. without written consent. Page 14 International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 .when which then allows the subject. (including If there is any uncertainty regarding the photographs). giving parental permission. USC 241 (d)). g. Approval Form (1B) where applicable. web based surveys) are allowed but will pose challenges in a) collecting anonymous data. medical following: centers.. universities. Student Checklist (1A) • As part of the process of obtaining informed consent. See the online Risk Assessment Guide for more detailed procedures. Any and all use and distribution of the test must be in accordance with the publisher’s requirements including procurement of legal copies of the instrument. The gathering personal information. IRB Waiver of Written Informed Consent • Informed consent may not involve coercion and is an on- The IRB may waive the requirement for documentation going process. Regulated Research Institution Form (1C) . 42.g.g. to oversee the project. the c. if the research involves only minimal risk and 6) Research conducted by a pre-college student at federally anonymous data collection and if it is one of the regulated research institutions (e.. NIH. Human Subjects Form (4) benefits associated with participation in the research study f. they may decide whether or not to participate) and that they are free to stop participating at any time. Checklist for Adult Sponsor (1) informed consent. approved by the IRB. not a single event that ends with a signature of written informed consent/assent/parental permission on a page. The IRB must confirm that the exists and where the probability and magnitude student is not violating the medical practice act of the of harm or discomfort anticipated in the research particular state or nation in which he/she is conducting the are not greater than those ordinarily encountered research. correctional institutions. See Risk Assessment below and the 12) The following forms are required: online Risk Assessment Guide for further explanation of a. etc. in DAILY LIFE or during performance of routine 8) Student researchers may NOT publish or display information physical activities. parents or guardians to make applicable an educated decision about whether or not to participate. Qualified Scientist Form (2) . Research Plan researcher will provide information to the subject (and d.when applicable • Participants will also be informed that their participation is voluntary (i.e. the SRC reviews and approves previously must be written depending on the level of risk and the type of approved projects to make sure that students followed study and will determine if a Qualified Scientist is required the approved Research Plan and the rules.) must be reviewed a) Research involving normal educational practices and approved by that institution’s IRB. Scientist as required by the instrument publisher.. email. it is strongly 9) All standardized tests that are not in the public domain must recommended that documentation of written informed be administered. assent/parental permission. questionnaires. A letter from the mentor is not sufficient than minimal risk. b. invasion of qualified professional must be named in the research protocol privacy or potential for emotional distress. c) Surveys. in a report that identifies the human subjects directly or through identifiers linked to the subjects. medical procedures and medication administration only under cognition.

learning disorders. etc. For example.g. Risk level can be reduced by protecting confidentiality or collecting data that is truly anonymous. anxiety or low self-esteem or viewing violent or distressing video images. students receiving services under the Individuals with Disabilities Education Act). anxiety. prisoners. individuals with diseases such as cancer. answering questions that could result in feelings of depression. c. depression. These studies should require documented informed consent/minor assent/parental permission (as applicable).org/isef/primer/rules. mentally disabled persons. Ingestion. a. viewing of stimuli) or experimental condition that could potentially result in emotional stress would be considered more than minimal risk. answering questions related to personal experiences such as sexual. children/minors. See the online Risk Assessment Guide for a more detailed discussion of Risk Assessment. Special groups that are covered by federal regulations. physical or child abuse. asthma. Any member of a group that is naturally at-risk.g. diabetes. Anonymity involves collecting research in such a way that it is impossible to connect research data with the individual who provided the data. (e. divorce. the IRB and student research must consider whether the nature of the study requires special protections or accommodations. ingestion or tasting projects that involve commonly available food or drink will be evaluated by the IRB who will determine risk level based upon the nature of the study and local norms.) b. However. or application of a substance would typically be considered more than minimal risk. AIDS. dyslexia. pregnant women. questionnaire. tasting. 1) Physical Risks a. Risk Assessment No more than minimal risk exists when the probability and magnitude of harm or discomfort anticipated in the research are not greater (in and of themselves) than those ordinarily encountered in DAILY LIFE or during performance of routine physical or psychological examinations or tests. economically or educationally disadvantaged persons. More than minimal risk exists when the possibility of physical or psychological harm or harm related to breach of confidentiality or invasion of privacy is greater than what is typically encountered in everyday life.g. survey. Exposure to any potentially hazardous material would be considered more than minimal risk. cardiac disorders. psychiatric disorders. smelling. 2) Psychological Risks A research activity (e. (e. www.asp International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 Page 15 . Protecting confidentiality involves taking measures to ensure that identifiable research data are not disclosed to the public or unauthorized individuals. Exercise other than ordinarily encountered in DAILY LIFE would be considered more than minimal risk b. 3) Invasion of Privacy The student researcher and IRB must consider whether any activity could potentially result in negative consequences for the subject due to invasion of privacy or breach of confidentiality.societyforscience. pregnant women. 4) Risk Groups If the research study includes subjects from any of the following groups.

1-800-374-2721 http://www. 866-447-4777 email: ohrp@osophs. Can be purchased from: http://www.apa.gov Page 16 International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 .gov/guidelines/belmont. Washington. C. MA: Thomson Centerwatch. M. toll free in U.org http://www.html 2) Dunn. (1993). April 18.apa..nih. Ext. L. If outside US.nih. Suite 200 Rockville. DC 20002-4242 phone: 202-336-5500. 8087 http://www. NCME. Washington.org Information for students: http://www.com NIH tutorial also provides similar information: http://www.gov/guidelines/45cfr46.html 6) American Psychological Association 750 First Street.L. Boston.apa.gov/clinicaltrials/learning/page2 3) Penslar.html Information regarding publications: http://www. Title 45 (Public Welfare).od.gov/ohrp/irb/irb_guidebook.apa. DC: AERA. R.amazon. NE Washington.html Many of the documents above are also available by contacting: Office for Human Research Protections Department of Health and Human Services The Tower Building 1101 Wootton Parkway. Part 46-Protection of Human Subjects (45CFR46) http://ohsr. APA. Institutional Review Board (IRB) Guidebook.Sources of Information 1) Code of Federal Regulation (CFR). To order call: (800) 628-4094. G.org/science/infostu. 1979 http://ohsr. call (717) 632-3535.dhhs.cancer. (1999).hhs.apa. and Chadwick.html 5) Standards for Educational and Psychological Testing.org/science/testing. MD 20852 phone: 240-453-6900.org/science/standards.. DC: ORRP-NIH http://www.htm 4) Belmont Report.od.S. ISBN 1- 930624-36-0.org/publications/ 7) Educational and Psychological Testing Testing Office for the APA Science Directorate phone: 202-336-6000 email: testing@apa. Protecting Study Volunteers in Research: A Manual for Investigative Sites (2002).

protect the welfare of investigated. Studies of pain • All federal or state fish. See Section B. Explain the potential impact or • Reduce the number of animals without compromising contribution this research may have on the broad fields statistical validity of biology or medicine. conditioning with aversive stimuli.) 11) After initial SRC approval. bird and reptile eggs within three days (An IACUC is the review and approval body at a regulated (72 hours) of hatching. therefore. A death rate of 30% or greater in any group or well-being of the animal subjects must be considered. (Note: Humane injury to the animals during the course of euthanasia is permitted under certain conditions when the experimentation. pain and to kill vertebrate animals are prohibited. a Qualified Scientist or Vertebrate Animals Designated Supervisor must directly supervise all research involving vertebrate animals. 6) If an experimental design requires food or water restriction. 13) Certain types of vertebrate animal studies may be 5) Because weight loss is one significant sign of stress. the experiment must be immediately students conduct research with animal subjects. age. the conducted at home. lower considered. International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 Page 17 . • Refine the experimental protocol to lessen pain or b. state. Predator/vertebrate prey experiments regulations are followed. tissue/cell cultures or computer simulations unacceptable. weight. plan for vertebrate animal studies must include the following: 2) Alternatives to the use of vertebrate animals for research a. federal regulations. Rules for ALL Studies Involving 9) Except for observational studies. Vertebrate animals. whereas other studies must be conducted (compared to controls) of any experimental or control at a regulated research institution. nonhuman vertebrate mammalian embryos must approve all research before experimentation begins. how the animals will be used. Include distress to the animals. distress. herbicide. source and number of animals proposed for use. Describe in detail. the health and terminated. but may not exceed 18 hours. tadpoles. • There is no interaction with the animals being b. as covered by these rules. Describe the procedures that will or suffering to vertebrate animals or which are designed minimize the potential for discomfort. insecticide. or heavy exempt from prior SRC review if ALL of the following apply: metals. All projects involving vertebrate animals must adhere to the rules below AND to either Section A or Section B rules 8) Students performing vertebrate animal research must depending on the nature of the study and the research site. for the choice of species and the number of animals to be Alternatives include the following “3 R’s”: used.S. strain. including the reasons must be explored and discussed in the research plan. and the reasons these alternatives were life forms. Justify why animals must be used. including 12) Studies involving behavioral observations of animals are alcohol. research is conducted at a regulated research institution. Research Site and B. methods and procedures. a student with any proposed changes in the Research Plan of the project must repeat the 4) The following types of studies on vertebrate animals are approval process before laboratory experimentation/data prohibited: a. Regulated Research Site below for rules and site descriptions. subgroup is not permitted and the project will fail to qualify for competition. it must be appropriate to the species. such as experimental design 3) Research projects which cause more than momentary pain and data analysis.) The research (including fish) at hatching or birth. Non-regulated animal is 15%. toxin that could impair health or destroy life. the cause of the death must be professional scientists and to. Identify the species. 1) The use of vertebrate animals in science projects is allowable under the conditions and rules in the following 10) A Scientific Review Committee (SRC) and/or an sections. See A. game and wildlife laws and d. Behavioral experiments involving operant observed. All induced toxicity studies involving a poison or collection resumes. If the experimental procedure is responsible both animal subjects and the student researcher. or fetuses. acid rain. country and U. mother/infant • There is no manipulation of the environment in any separation or induced helplessness way and c. When for the deaths. Describe any alternatives to animal use that were • Replace vertebrate animals with invertebrates. sex. Vertebrate Animals The following rules were developed to help pre-college student 7) If there are unexpected deaths in either the experimental or researchers adhere to the federal regulations governing control groups. are Institutional Animal Care and Use Committee (IACUC) defined as live. and all other nonhuman vertebrates research institution for all animal studies. follow local. school or other non-regulated maximum permissible weight loss or growth retardation research sites.

the affected conducted under the responsibility of a principal animals must have proper medical and nursing care that is investigator. ventilated. veterinarian is required to certify that the research plan and animal husbandry are appropriate. Fish may be obtained from the wild • Studies involving animals in their natural environment only if the researcher releases the fish unharmed.). pens and fish tanks must be cleaned frequently. pharmaceutical and • Guide for the Care and Use of Laboratory Animals biotechnology companies that utilize research animals that are • Guide for the Care and Use of Agricultural Animals in not covered by the Animal Welfare Act but have an operational Agricultural Research and Teaching (Ag-Guide) Institutional Animal Care and Use Committee and program structured in compliance with U.A.) that involve supplemental nutrition. is not permitted for a project conducted in a non-regulated observational or supplemental nutritional studies on site. animals. only be resumed if the cause of illness or death is not related to the experimental procedures and if appropriate steps are taken to eliminate the causal factors. and vacation periods. This certification is (NOTE: Some research that is permissible for professionals in required before experimentation and the prior SRC research institutions is not appropriate for pre-college approval.) 2) Animals must be treated kindly and cared for properly. b. The following and is licensed to use animals covered by the Animal Welfare documents offer space requirements and additional Act. A student researcher is expected certify that the research project complies with ISEF Rules. Approval Form (1B) (Note: All studies not meeting the above criteria must be e. clean (uncontaminated) water and Institution food supply. Page 18 International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 . federal laws are included in 3) The Scientific Review Committee must determine when a this definition. Research Plan animal’s health or well-being. Also included are all federal laboratories such as National husbandry information: Institutes of Health. Additional Rules for Projects environment compatible with the standards and Conducted in a Regulated Research requirements appropriate for the species used. They must be given a continuous. behavioral. The research involves only non-invasive and non.S. and adheres to state and local fishing laws • Studies involving livestock that use standard agricultural and regulations. A veterinarian must be consulted in experiments students. to stop experimentation if there is significant weight loss or This SRC review should occur before experimentation death in the experimental subjects. Additional Rules for Projects Conducted in a Non-regulated Site Vertebrate animal studies may be conducted at a non-regulated 5) Animals may not be captured from or released into the research site (home. ranch. must approve all student research projects before experimentation begins. Checklist for Adult Sponsor (1) b. has the • Studies involving animals in zoological parks proper license. administration of prescription drugs and/or activities that would not be 1) The Institutional Animal Care and Use Committee (IACUC) ordinarily encountered in the animal’s daily life. guidelines: Euthanasia for tissue removal and/or pathological analysis a. Animals must be housed in a clean. practices. The experiment can begins. The research involves agricultural. d. Student Checklist (1A) intrusive methods that do not negatively affect an c. AND 7) The following forms are required: a. conducted in a regulated research institution. Vertebrate Animal Form (5A) conducted at a Regulated Research Institution. A Designated Supervisor is required to oversee teaching institution that is regularly inspected by the USDA the daily husbandry of the animals. must be including weekends. This wild without approval of authorized wildlife or other includes: regulatory officials. Veteran’s Affairs Medical Centers and the • Federal Animal Welfare Regulation Centers For Disease Control. In addition. The local SRC must also review the project to directed by a veterinarian. when applicable Section B. below. Qualified Scientist Form (2). school. 6) The final disposition of the animals must be considered 1) These projects must adhere to BOTH of the following and explained on Vertebrate Animal Form (5A). comfortable B. Cages. holidays. See f. etc. A regulated Animals must be observed daily to assess their health and research institution is defined as a professional research/ well-being. farm. Such research projects must be 4) If an unexpected illness or emergency occurs. in the field. Proper care must be provided at all times All studies not meeting the criteria in Section A.

Lockbox 285 Category C Live animals will have significant Permitted only Washington. Most procedures. II.fisheries. Approval Form (1B) AVMA Guidelines on Euthanasia (June 2007) e.html b. National Research http://dels. Can be found online: http://dels. f.avma. NW husbandry practices. 5) The following forms are required: American Fisheries Society. blood National Academies Press sampling and standard agricultural 500 Fifth Street. inoculation or Federation of Animal Science Societies (FASS) exposure to unknown or potentially hazardous materials or 1111 N. Unit 84 STUDIES MUST INCLUDE TREATMENT for high school students. http://awic.org such action is feasible.edu procedure without regaining consciousness. Vertebrate Animal Form (5B) http://www. ALL SUCH inappropriate 4700 River Road.C. Dunlap Avenue drugs is permitted to proceed only to the point where the first Savoy. a.gov/awic/legislat/awicregs. etc.usda. surgery. tranquilizers or analgesics. cells.shtml 3) Research projects that cause more than momentary pain or suffering to vertebrate animals are prohibited. 2131-2157 develop discernable clinical signs proper training Subchapter A .org euthanized.shtml USDA Pain Definition ISEF Categories Guidelines 3) Guidelines for the Care and Use of Mammals in Category A Live animals will receive non-painful Permitted manipulation. Regulated Research Institution Form (1C) American Veterinary Medical Association. field trapping/tagging.S. Animals may be euthanized Neuroscience and Behavioral Research (2003). Research Plan 7) Euthanasia Guidelines d. If not. performed with proper phone: 888-624-8373 or 202-334-3313 while anesthetized.org/afs/publicpolicy.edu/ilar_n/ilarhome/reports.edu/ilar_n/ilarhome/reports. Category D Live animals will have manipulations Limited Category 4) Federal Animal Welfare Act (AWA) performed while anesthetized and are D procedures are allowed to recover and/or animals will permitted with 7 U. distress.nas. which results in a short-term response. Commission on Life Sciences.gov 5) Guide for the Care and Use of Agricultural Animals in Agricultural Research and Teaching (Agri-Guide) 4) Research in nutritional deficiency. Checklist for Adult Sponsor (1) http://www. etc. The animals will be training and euthanized at the termination of the certification fax: 202-334-2451. To order these ILAR publications contact: Examples include but are not limited to: injections. indicating pain.usda. Appropriate measures phone: (217) 356-3182 must then be taken to correct the deficiency or drug effect. email: ace@aphis. III) and certification.pdf International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 Page 19 . All methods of euthanasia must Institute of Laboratory Animal Research (ILAR). for Laboratory Animal Research (ILAR).htm physiological changes spontaneously or as adhere to all ISEF a result of specific experimental rules. The following 2) Principles and Guidelines for the Use of Animals in table relates the USDA Pain Categories and the permissibility Precollege Education (a free pamphlet from ILAR) of studies for science fair projects.nal. the animal(s) must be http://www. DC 20055 manipulations.2) Proper euthanasia at the end of experimentation for tissue Sources of Information for removal and/or pathological analysis is permitted. without Fax: (301) 734-4978 benefit of anesthetics.usda. Examples include.fass. but are not Category D Above document is available from: limited to: Survival surgical procedures of projects would any type and some studies which would be deemed USDA/APHIS/AC include tumor development. perform the euthanasia.nas. or significant The project must http://www. ingestion.gov Category E Live animals will experience PROHIBITED Tel: (301) 734-7833 significant/severe pain or distress. 6) Guidelines for the Use of Fish in Research (2004). if email: fass@assochq.. IL 61874 sign of the deficiency or effect appear. http://www. Student Animal Care and Use reseachers are prohibited from performing euthanasia.nap. Qualified Scientist Form (2) euthanasia. Category B Live animals will receive momentary pain Permitted or stressful stimulus without anesthesia. MD 20737-1234 TO ALLEVIATE PAIN OR DISTRESS.Animal Welfare (Parts I. Institute to obtain tissues. only the Qualified Scientist or an institutional representative may 1) Guide for the Care and Use of Laboratory Animals. adhere to current AVMA Guidelines.nal.org/issues/animal_welfare/ g. Riverdale. Student Checklist (1A) c.

jhsph.gov.edu 1-888-FIND-NLM or 1-888-346-3656 http://caat. housing and handling standards.gov http://www.nih. and alternatives to animal use through annotated bibliographies published quarterly in ILAR Journal. MD 20892-5467 Ph: 301-496-1131.usda.nlm.Sources of Information for Alternative Research and Animal Welfare 1) The National Library of Medicine provides computer 5) John’s Hopkins Center for Alternatives to Animal Testing searches through MEDLINE: (CAAT) has worked with scientists since 1981 to find new Reference & Customer Services methods to replace the use of laboratory animals in National Library of Medicine experiments.nih.nlm.nlm.gov/altbib.gov http://www.edu http://dels.html Page 20 International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 . MD 20705-2351 phone: (301) 504-6212. Keck 687 Washington. Bethesda. NW.gov http://www.nih.nih.nih.edu/ (301) 594-5983.gov/entrez/query.nas.gov/awic 3) Institute of Laboratory Animal Resources (ILAR) provides a variety of information on animal sources. DC 20001 phone: (202) 334-2590.nih.gov http://www. Room 410 Beltsville.fcgi 2) National Agriculture Library (NAL) provides reference service for materials that document a) Alternative Procedures to Animal Use and b) Animal Welfare. Suite 510 6707 Democracy Blvd.usda. reduce the number of animals tested.nlm. Animal Welfare Information Center National Agriculture Library 10301 Baltimore Avenue.nal. MD 20894 email: caat@jhsph. email: custserv@nlm.ncbi.sis.edu/ilar/ 4) Quarterly bibliographies of Alternatives to the Use of Live Vertebrates in Biomedical Research and Testing may be obtained from: Specialized Information Services NLM/NIH 2 Democracy Plaza. ILAR The Keck Center of the National Academies 500 Fifth Street. Fax: 301-480-3537 Toll Free: 1-888-FIND NLM or 1-888-346-3656 Email: tehip@teh. MSC 5467 Bethesda. http://toxnet.. fax: 202-334-1687 email: ILAR@nas. fax: (301) 504-7125 email: awic@nal. and refine 8600 Rockville Pike necessary tests to eliminate pain and distress.nlm.

blood. blood. rDNA. fungi. Require completed Risk Assessment Form 3: collected at home as long as they are immediately transported 1) Studies involving protists. The student researcher must receive All projects involving microorganisms. Students are permitted adult supervisors prior to experimentation and a final to do research projects with potentially hazardous biological biosafety level must be determined or confirmed by the SRC. but may not be introduced into a home/ DNA (rDNA) technologies or human or animal fresh/frozen garden environment. researcher and adults supervising the project must be nitrogen-fixing. blood or supervised while conducting microbiological procedures. The following rules were developed to protect be conducted in a BSL-1 or higher laboratory. 7) Naturally-occuring plant pathogens may be studied (not viroids. MUST be conducted in a laboratory rated BSL-2 or above (Form 6A) to define the potential level of harm. The research must be supervised by a project to proceed. and human and vertebrate animal tissues) Projects involving microorganisms (including bacteria. All of 13) The following types of studies are exempt from prior SRC these areas of research may involve potentially hazardous review: biological agents and require special precautions. and parasites). viruses. to the additional rules in Section A. and supervision required for the research not require review. use of 10% sodium hypochlorite. 3) Commercially-available color change coliform water 5) Laboratory stuides utilizing MRSA (Methicillin resistant test kits which will remain sealed and will be properly Staphlococcus aureus) and VRE (Vancomycin-resistant disposed. specimens are allowed to be B. The research students and to help them adhere to federal and international must be supervised by a trained Designated Supervisor or a biosafety regulations and guidelines. Qualified Scientist. prohibited for precollege students. rickettsia. is prohibited in a environment that could potentially be contaminated). prions. or body fluids is including sewage sludge. alkaline hydrolysis. from an institutional representative that the research does equipment. resistant organisms. incineration. injury or disease (commonly found in a regulated research institution). A. depending on 11) All potentially hazardous biological agents must be the study. oil-eating bacteria. 23. 1) Studies involving baker’s yeast and brewer’s yeast. B or C. body fluids must adhere to the rules below AND. safely and that the projects meet the conditions and rules 9) Research determined to be at Biosafety Level 1(BSL-1) may described below. recombinant DNA (rDNA) technologies or human 12) Studies involving the culturing of human or animal waste. 2) Research using manure for composting or other non- 4) Research determined to be biosafety levels 3 or 4 is culturing experiments and fuel production. archae and similar to a laboratory with the appropriate level of biosafety microorganisms containment. Bacillus thurgensis. The risk assessment determines a final Institutional Biosafety Committee (IBC) or a letter obtained biosafety level which then determines the laboratory facilities. However. and recommendations. Following are Rules for ALL Studies Involving acceptable procedures for disposal of cultured materials: Potentially Hazardous Biological Agents Autoclaving at 121 degrees Celsius for 20 minutes. Studies using such organisms require at least BSL-2 containment. Potentially Hazardous Biological Agents (includes rules involving microrganisms. recombinant DNA extensive training. or body fluids may involve working with 8) A risk assessment must be conducted by the student and potentially hazardous biological agents. viroids. Qualified Scientist. even BSL-1 organisms. The initial risk assessment determined by the student 2) Studies involving Lactobacillus. No additional forms required: 2) An appropriate review and approval committee (SRC. enterococci) are prohibited. demonstrate competency and be directly technologies and human or animal fresh/frozen tissues. prions. parasites). allowable under the conditions and rules that follow. See page 23. IACUC) must approve all research before experimentation except when involved with rDNA studies begins. The student must be properly trained in When dealing with potentially hazardous biological agents it is standard microbiological practices. fungi. 14) Any proposed changes in the Research Plan by the 6) Studies intended to genetically engineer bacteria with student after initial SRC approval must have subsequent multiple antibiotic resistance are prohibited. or animal fresh/frozen tissues. IBC. agents as long as every effort is made to ensure that they work See p. training. tissues. rickettsia. viruses. (Not exempt if cultured in a petri dish biological agents. International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 Page 21 . slime mold and confirmed by the SRC. recombinant cultured) at home. home environment. the responsibility of the student and all of the adults involved in 10) Research determined to be a Biosafety Level 2 (BSL-2) a research project to conduct and document a risk assessment. algae-eating bacteria introduced into their natural 3) Experimentation involving culturing of potentially hazardous environment. properly disposed of at the end of experimentation in accordance with their biosafety level. Extreme SRC or IBC review and approval before such changes are caution should be exercised when selecting out antibiotic made and before experimentation resumes. The to plants. must be treated as a BSL-2 study. 1) The use of potentially hazardous microorganisms (including biosafety pick-up and other manufacturer bacteria. animals and humans that may occur when working with research must be reviewed and approved by the biological agents.

subtilis host-vector 6) Human breast milk of unknown origin. acceptable containment include doubled heavy-duty (2-ply) pg 17. blood contaminated instruments) SRC prior to experimentation. DNA in E. household original animal study from which tissues are obtained is surfaces. supervised by a Qualified Scientist or trained Designated 2) If a culture container is opened for any purpose. it must be Supervisor.e. because the presence.15) The following forms are required: 4) All rDNA technology studies involving BSL-2 organisms and/ a. Qualified Scientist (2). to experimentation. Examples include cloning of must be properly disposed of after experimentation. be handled in accordance with standards and guidelines set 1) All rDNA technology studies involving BSL-1 organisms and forth in the OSHA. Recombinant DNA (rDNA) Technologies 5) All studies involving human or wild animal blood or blood Studies involving rDNA technologies in which microorganisms products should be considered a Biosafety level 2 study and have been genetically modified require close review to assess risk must be conducted in a BSL-2 laboratory under the supervision level assignment. These studies must B. Qualified Scientist or trained Designated Supervisor and must 7) Any study involving the collection and examination of body be approved by the SRC prior to experimentation. All blood must review by a knowledgeable SRC. blood products. Research Plan. S. concentration and pathogenicity of 1) If tissues are obtained from an animal that was sacrificed for a possible agents are unknown. identified with a specific person. 29CFR. it may be considered studies typically involve the collection and culturing of a tissue study. blood or body fluids obtained from humans and/or vertebrate may contain Microorganisms microorganisms and have the potential of causing disease. Studies involving unknown microorganisms present a challenge Therefore. BSL-1 study under the following conditions: the study must be considered a vertebrate animal project and a) Organism is cultured in a plastic Petri dish (or other adhere to the vertebrate animal rules for studies conducted at a standard non-breakable container) and sealed. 3) Biosafety level 1 studies involve the collection and examination b) Experiment involves only procedures in which the Petri dish of fresh/frozen tissue and/or body fluids. Any tissue or BSL-1 host vector systems may be conducted in a BSL-1 instruments with the potential of containing bloodborne laboratory under the supervision of a Qualified Scientist or pathogens (eg. In science fair projects these purpose other than the students’ project.) required. must have IRB review and informed consent. Pasteurized domestic animal milk may be be conducted in a BSL-1 laboratory under the supervision of a considered BSL-1.g. Additional Rules for Projects Involving be conducted in a regulated research institution in a BSL-2 laboratory under the supervision of a Qualified Scientist. All studies involving domestic animal safely conducted in a BSL-1 high school laboratory with prior blood may be considered a BSL-1 level study. c. (See the vertebrate animal rules. PHBA Risk Assessment Form (6A) viruses) is prohibited. including Blood and Blood Products A. and Approval Form (1B) regulated research institution and approved by the IBC prior b. plant or animal toxins (including e. of fresh/frozen tissues or body fluids that may contain microorganisms belonging to BSL-1 or 2. where the sample can be experimentation must be conducted entirely in a BSL-2 facility. Regulated Research Institution Form (1C) . coli K12. blood. HIV and Hepatitis C and domestic unpasteurized animal milk are 2) Commercially available rDNA kits using BSL-1 organisms may considered BSL-2. fluids which may contain biological agents belonging to BSL-3 3) A rDNA technology study that involves BSL-1 agents that or 4 is prohibited. Subpart Z. Students using their own body fluids are exempt from this requirement.) sealed bags. Additional Rules for Projects Involving Unknown Studies involving fresh/frozen tissue. and B.when appl. Other regulated research institution. Human and Vertebrate Animal Tissue Form (6B) – for all studies involving tissues and body fluids. Student Checklist or BSL-2 host vector systems must be conducted in a (1A). etc. Page 22 International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 . Risk Assessment (3). f. soil. treated as a BSL-2 study and involve BSL-2 laboratory 4) Biosafety level 2 studies involve the collection and examination procedures. Additional Rules for Projects Involving Tissues & Body Fluids. Checklist for Adult Sponsor (1). when applicable 5) Propagation of recombinants containing DNA coding for d. unless certified free of systems. counting or blood products. see rule 5) from a non-infectious source with presence of organisms or colonies). Documentation of the IACUC approval for the microorganisms from the environment (e. tissues that release trained Designated Supervisor and must be approved by the blood when compressed. (not including blood remains sealed throughout the experiment (i. when applicable oncogenes or other human. There are a few rDNA studies that can be of a Qualified Scientist. may convert to BSL-2 agents during the course of 8) Studies of human body fluids. a proper risk assessment is required. skin. Biosafety level 1 c) The sealed Petri dish is disposed of in the appropriate studies can be conducted in a BSL-1 laboratory and must be manner under the supervision of the Designated Supervisor. cerevesiae. C. little likelihood of microorganisms present. 1) Research with unknown microorganisms can be treated as a 2) If the animal was euthanized solely for the student’s project.

supervised by a competent scientist who understands the risk BSL-3 risk group contains biological agents that usually associated with working with the agents involved. and in colleges teaching BSL-1 risk group contains biological agents that pose low introductory microbiology classes. Prepared fixed tissue American Type Culture Collection). The laboratory work is supervised by an Micrococcus leuteus. are classified according to There are four levels of biological containment (Biosafety biosafety level risk groups. injury or experimentation. training.g. school). Fossilized tissue or archeological specimens b. The laboratory work must be Streptococcus pneumonia. obtained from the g. in high schools. PROHIBITED International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 Page 23 . animal or plant) or that can result in serious economic consequences. the to a biosafety level risk group based on information final biosafety level must be confirmed by the SRC. The agents require Biosafety can be achieved by treating with chemical disinfectants or by Level 1 containment. Work is done on an open risk to personnel and the environment. animal or plant) that is often untreatable. Agrobacterium tumifaciens. the SRC available to the student researcher to conduct the should review the project and assign a final biosafety level. Neurospora crassa. protection are required. plant or animal. Lab coats are required and gloves Escherichia coli strain K12. gloves and face Examples of BSL-2 organisms are: Mycobacterium. laboratories. The end result of a risk • Assessment of the experience and expertise of the adult(s) assessment is the assignment of a final biosafety level which supervising the student. Plant tissue f. or packing houses d. the final biosafety level o The study of unknown microorganisms and the use of must be assigned by an Institutional Biosafety Committee (IBC) fresh tissues should rely on the expertise of qualified or equivalent approval body or a letter obtained from an adults supervising the project.) working with biological agents. animals or plants. available. The source and catalog Risk Assessment (Use this information to complete PHBA Risk Assessment Form 6A) Risk assessment defines the potential level of harm. If the available through a literature search. level of biological Risk assessment involves: containment available and the expertise of the Qualified • Assignment of the biological agent to a risk group Scientist or Designated Supervisor who will be supervising o Studies involving a known microorganism should the project. ordinary circumstances in the research laboratory. institutional representative that the research does not require • Determination of the level of biological containment review. the risk of spread working with agents of moderate risk to humans and the is limited and it rarely would cause infection that would lead environment. animals and humans that may occur when Containment below for more details. BSC) must be measures are available in the event that an infection occurs. These agents are bench or in a fume hood. e. Salmonella choleraesuis. An autoclave should be readily available for The agents require Biosafety Level 2 containment. Teeth that have been sterilized to kill any blood borne pathogen 10) The following types of tissue do not need to be treated as that may be present. Established cell and tissue cultures (e. If no approval body exists at the regulated site. cause serious disease (human. These classifications presume Level 1 . PROHIBITED BSL-3 containment is required for infectious agents that may cause serious or potentially lethal diseases as a result of BSL-4 risk group contains biological agents that usually exposure by inhalation. type A. Each level has guidelines for laboratory facilities. Decontamination workers.9) Studies involving human embryonic human stem cells must be number of the cultures should be identified in the Research Plan conducted in a registered research institution and reviewed c. Chemical disinfection or autoclaving at potentially hazardous biological agents: 121 degrees Celsius for 20 minutes is a recommended procedure. recommended. If BSL-2 containment is designed to maximize safety when exposure occurs in a laboratory situation. Hair Oversight) Committee. Classification of Biological Agents Risk Groups Levels of Biological Containment Biological agents.4). a. of agents in small volumes for diagnostic and experimental BSL-1 containment is normally found in water-testing purposes. Lab coats.. and approved by the ESCRO (Embryonic Stem Cell Research restaurants. decontaminating waste materials. equipment. (Please see Levels of Biological disease to plants. PROHIBITED BSL-4 containment is required for dangerous/exotic agents that pose high risk of life-threatening disease. then determines the laboratory facilities. individual with general training in microbiology or a related BSL-2 risk group contains biological agents that pose science. or growth safety equipment and laboratory practices and techniques. Access to the laboratory is restricted. research is conducted at a regulated site. on risk group of biological agent. begin with an initial assignment of the microorganism If a study is conducted at a non regulated site (e. Examples of BSL-1 organisms are: steam autoclaving. Effective treatment and preventive Biological safety cabinets (Class 2. to serious disease. Bacillus subtilis. moderate risk to personnel and the environment. Standard microbiological practices highly unlikely to cause disease in healthy laboratory are used when working in the laboratory. Meat or meat by-products obtained from food stores. PROHIBITED produce very serious disease (human. • Assignment of a final biosafety level for the study based and supervision required for the research project to proceed.g.

nih.gov The Mad Scientist Network at Washington University School of Medicine: http://www. Includes risk assessment and safe use of recombinant DNA technology. 1(800) 638-6597 (US. French. Provides practical guidance on biosafety techniques for use in laboratories at all levels. & Portuguese.int/csr/bioriskreduction/ Available online in English.osha.org Biosafety in Microbiological and Biomedical Laboratories (BMBL) . and provides guidelines for the commissioning and certification of laboratories.od. & PR) http://www. Published by National Institutes of Health. To order: Office of Health and Safety Centers for Disease Control and Prevention 1600 Clifton Road.phac-aspc.bergeys. Spanish. Canada.4th Edition.absa.html OSHA – Occupational Health and Safety Administration http://www. Canada – Agency of Public Health – list of non-pathogenic organisms http://www.gov/oba/index.htm World Health Organization Laboratory Safety Manual-3rd Edition http://www. GA 30333 http://www. Mailstop F05 Atlanta.org American Type Culture Collection (703) 365-2700.madsci.com/safemicrobes.cdc. http://oba.Sources of Information American Biological Safety Association: ABSA Risk Group Classification – list of organisms http://www.gov/od/ohs/biosfty/biosfty.html Microorganisms for Education Website – list of organisms http://www.org Bergey’s Manual of Systematic Bacteriology website – follow the links for resources and microbial databases for a collection of international websites of microorganisms and cell cultures: http://www.atcc.ca/ols-bsl/pathogen/ organism_e.gc.org Page 24 International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 .science-projects.htm NIH Guidelines for Research Involving Recombinant DNA Molecules.who. NE. Published by CDC-NIH.

Students outside of the U. restriction for purchase. Many such products have an age manner in accordance with good laboratory practices. Qualified Scientist Form (2) . Alcohol & Tobacco of risk above and beyond that encountered in the student’s D. Schedule 2. There are additional rules for the following regulated country. A 2) Administering any prescription drug to vertebrate animals copy of the permit should be available for review by adults must be done under all appropriate vertebrate animal rules supervising the project and/or the Scientific Review and guidelines. DEA-controlled substances guidelines may be in effect. prescription drugs. a.when 1) Production of ethyl alcohol (wine or beer) is allowable in applicable the home under the supervision of the parents and must f. Activities and Devices of DEA-controlled substances. Research Plan usage and appropriate disposal of the alcohol or tobacco used d. must additionally adhere to their 6) The following forms are required: local and country laws and regulations. he/she should consult the listing Chemicals. Other countries may have similar precautions are taken. Hazardous activities are those that involve a level C. firearms and explosives. (see p.S. for The U. A. be sure to check the drug regulatory agency in their country in addition to with your school. Prescription Drugs substances in accordance with all local. 3 and 4 substances do not require Scientist prior to experimentation. e. documented on the Risk Assessment Form (3).S. federal Prescription drugs are drugs regulated by federal or country and country laws. A. etc. radiation. Approval Form (1B) in the study. DEA-controlled Substances prescription drugs. local. Prescription Drugs explosives. except those DEA (or other appropriate international regulatory body) involving DEA-controlled substances which require for use of the controlled substance. 2) All studies using DEA Schedule 1 substances must have 2) The student researcher must conduct a risk assessment in the research protocol approved by DEA before research collaboration with a Designated Supervisor or Qualified begins. the student/supervisor will be expected drugs to human subjects.S. DEA-Controlled Substances These rules are intended to protect the student researcher by The U. Activities or Devices (Includes DEA-controlled substances. devices and activities. 14) to have the permit prior to the onset of experimentation. alcohol and tobacco and firearms and B. Firearms and Explosives everyday life. and their schedule number can be found at the DEA website listed in the Sources of Information at the end of the section.S. Therefore. lasers. This risk assessment is protocol approval by DEA. U. (see p. 4) For all chemicals. If Rules for ALL Projects Involving Hazardous a student is uncertain whether chemicals involved in a project are controlled by the DEA. or regional fair as more strict rules and being aware of DEA regulations. alcohol & tobacco. or international law. 17) A veterinarian is required. 3) Student researchers must acquire and use regulated B. should consult hazardous chemicals. activities or devices. devices or activities requiring a Federal 1) Students are prohibited from administering prescription and/or State Permit. 5) The student researcher must design experiments to minimize C. The rules include substances and devices that are regulated by local. Drug Enforcement Administration (DEA) regulates a ensuring that the proper supervision is provided and that all number of chemicals that can be diverted from their regular potential risks are considered so that the appropriate safety use to make illegal drugs. supervision by a Qualified Scientist. Student Checklist (1A) The Designated Supervisor is responsible for the acquisition.S. possession and consumption.) The following rules apply to research that involves the use of Additional Rules for Specific Regulated Substances hazardous chemicals. g. contact the appropriate against inappropriate or unsafe use. Hazardous Chemicals.when applicable meet the TTB home production regulations. special regulatory agencies listed below. Committee in their review prior to competition. most often with restrictions of substances: their use by minors such as DEA-controlled substances. Risk Assessment Form (3) International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 Page 25 . Alcohol and Tobacco the impact that an experiment has on the environment. For further information or classification for laws and are available only through a pharmacy to protect these laws and regulations. Regulated Research Institution Form (1C) . precautions must be taken in their use for a science project. state. c. state. Alcohol and Tobacco Tax and Trade Bureau (TTB) instance using minimal quantities of chemicals that must regulates the production of alcohol and distribution of alcohol subsequently be disposed of in an environmentally safe and tobacco products. 1) The use of hazardous chemicals and devices and 1) All studies using DEA-controlled substances must be involvement in hazardous activities require direct supervised by a Qualified Scientist who is licensed by the supervision by a Designated Supervisor. students outside of the U. Before beginning research involving regulatory bodies. Checklist for Adult Sponsor (1) b.

Radiation devices (Bunsen burners. They must be treated as hazardous devices. storage and other requirements of • Use safer solvents and reaction conditions federal explosive laws and regulations. reload kit or propellant • Use renewable materials modules containing more than 62. flammability or of other potentially dangerous devices such as high vacuum corrosiveness. Firearms and Explosives the chemicals used in an experiment. an 3) Students are allowed to conduct science fair experiments individual’s allergic and genetic disposition may have an involving the distillation of alcohol for fuel or other non. that require a moderate to high level of B. • Use safer chemicals and products • Design less hazardous chemical syntheses 2) A fully assembled rocket motor. hot plates.g.) may not A. injected or in contact with assessment. the work must be student researcher must refer to Material Safety Data Sheets conducted at school and a TTB permit must be obtained by (MSDS) to ensure that proper safety precautions are taken.2) Fermentation studies in which minute quantities of ethyl When doing a risk assessment the type and amount of alcohol are produced are permitted. reactivity. heated oil baths.. explosive is any chemical compound. to do so. The chemistry is to avoid the use or production of hazardous use of a firearm. substances during chemical process. when conducted with the direct supervision of a Designated Supervisor and when in compliance with all • Prevent waste federal. equipment. assuming that the A proper risk assessment of chemicals should include review of student researcher has experience working with the device. mixture or device. The mission of environmentally responsible (green) The purchase of a firearm by a minor is generally unlawful.5 grams of propellant are • Use catalysts subject to the permitting. to harm or destroy living tissues or physical equipment. the primary purpose of which is to function by explosion. influence on the overall effect the chemical may have. Explosives include. The consumable products. Hazardous Chemicals laboratory setting. A risk assessment must include proper disposal methods for D. Flinn) rank the degree of hazard available from the Alcohol and Tobacco Tax and Trade associated with a chemical. Page 26 International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 . black Environmentally Responsible Chemistry powder. An federal and state guidelines. swallowed. Some commonly used C. along with state agencies. without proper state certification. state and local laws. However.the tendency of a chemical to undergo chemical change Flammability – the tendency of a chemical to give off vapors which readily ignite when used under normal working conditions Corrosiveness – the tendency of a chemical. Whenever possible the following principles should be incorporated into the 1) Projects involving firearms and explosives are allowable research plan. Sources of Information section. For example. exposure to a chemical must be considered. school authorities. Hazardous Devices Guidance for Risk Assessment The documentation of a risk assessment (Form 3) is required Please find below guidance on conducting risk assessment when a student researcher works with potentially hazardous/ when using the following: dangerous equipment and/or other devices. Firearms and Explosives (referenced below) provides good information for the proper (ATF).S. The principles of Students should check the training and certification green chemistry are described on the EPA website in the requirements of individual states and countries. This rating may assist students Bureau (TTB) website referenced in the Sources of and adult sponsors in determining risk associated with the use Information section below. saws. • Increase energy efficiency Note: Potato guns or paintball guns are not firearms unless • Minimize the potential for accident they are intended to be used as weapons. NMR equipment. B. Details regarding this process are Some MSDS sheets (e. Bureau of Alcohol. but are not limited to. If applicable. and ignitors. drills. upon physical contact. Tobacco. in or outside a A. regulates the purchase and disposal of chemicals. pellet powder. and high Toxicity – the tendency of a chemical to be hazardous to temperature ovens must have documentation of a risk health when inhaled. Use factors such as the degree of toxicity. Hazardous Chemicals require a documented risk assessment. Reactivity . of a chemical. detonators. It is recommended that all student-designed the skin inventions also have documentation of a risk assessment. dynamite. A firearm is defined as a small incorporate in the research plan disposal procedure required by arms weapon from which a projectile is fired by gunpowder. etc. is illegal. the student researcher must use of firearms and explosives. Hazardous Devices expertise to ensure their safe usage. The Flinn Catalog The U.

usdoj. radiation released from these sources can be a health http://www.hhmi.gov/greenchemistry A risk assessment must be conducted when a student uses non-ionizing radiation beyond that normally encountered in Material Safety and Data Sheets (MSDS) everyday life. that includes a ranking of hazard level and disposal methods • Class I lasers are those found in CD players.gov 2. Washington.1 . Box 37535 Washington. fax: (202) 693-2498 Safety in Academic Chemistry Laboratories.gov/dea range finding devices and pose a risk if the beam is directly viewed over a long period of time. Volumes 1 and http://www. laser http://www. Nuclear Regulatory Commission Washington. They are to be schedules/schedules.A listing of numerous printers. (800) 368-5642 Safety in the Research Laboratory http://www.A Manufacturers are required to label Classes II – IV lasers.htm ionizing radiation beyond that normally encountered in everyday life.osha. They are Bureau of Alcohol.asp .cdc.ttb.com/msds/index.gov/ surgery. Non-ionizing radiation includes the spectrum of MSDS should be collected by your laboratory or available ultraviolet (UV).htm considered hazardous devices which can cause eye damage when the beam is directly viewed even for a Alcohol. Most research institutions have a Radiation Safety Office which oversees the use of ionizing radiation and ensures Occupational Safety and Health Administration Documents compliance with state and federal regulations.com/sections/safety/safety.usdoj.flinnsci. directory of MSDS sheets from Flinn Scientific Inc.gov/od/ohs/safety/S2.OSHA Coverage of Ionizing Radiation Sources Not American Chemical Society Covered by Atomic Energy Act of 1954 Publications Support Services 1155 16th Street.acs.ilpi.pdf hazard. MD 20852-2738 phone: (301) 415-8200. The internet also has a range of free radiofrequency (RF) and extremely low frequency (ELF). available from: OSHA Publications Sources of Information P. Controlled Substance Schedules – a list of controlled • Class III lasers are found in higher powered laser substances : http://www. Other free safety DVD’s are also available: order from the website: http://catalog. DC 20013-7535 General Lab/Chemical Safety phone: (202) 693-1888.gov A free DVD from Howard Hughes Medical Institute that includes sections on working with cell cultures. 11555 Rockville Pike website: http://www.gov/od/ohs/safety/SUPSAFE. Alcohol and Tobacco Tax and Trade Bureau • Class IV lasers are high powered lasers used in http://www.S. Firearms and Explosives extremely hazardous and can cause eye and skin http://www. Depending upon the level of http://www. aiming and http://www. from the manufacturer.atf. infrared (IR).cdc. research.html . http://www.O.gov damage from both direct and indirect exposure. DC: American Chemical Society. Radiation Environmental Protection Agency (EPA) website for green chemistry: http://www.C. microwave (MW). visible light. DEA Controlled Substances Drug Enforcement Agency website: • Class II lasers are found in laser pointers. Tobacco. 2003. NW U. The beam is also a fire hazard. There are no known risks associated with using a Class I laser.gov/ pointers. Projects involving radionuclides (radioisotopes) and X-rays must involve a careful examination of the risks CDC Laboratory Safety Manuals associated with the study.org/publications Rockville.PDF exposure.epa. radioactive materials and other laboratory materials. ultraviolet or infrared radiation. and industrial settings.Guidelines for Laser Safety and Hazard Assessment Order from (first copy free of charge): STD 1-4.jsp International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 Page 27 . DC 20036 Material Safety and Inspection Branch phone: (202) 872-4554 or 1-800-227-5558 One White Flint North email: pss@acs.nrc. Tobacco Firearms and Explosives short period of time.org/index. printers and spectrometers.7 . geological survey equipment and some sites that have free downloads of MSDS sheets laboratory equipment.deadiversion.cdc.org. Radiation Radiation Studies Information (CDC) A risk assessment must be conducted when a student uses http://www.gov/nceh/radiation/default. Lasers resources: usually emit visible. Lasers are classified into four classes based upon their safety. PUB 8-1.

manipulated. School Name. The abstract should include the Cellular & Molecular Biology following: Chemistry Computer Science a) purpose of the experiment Earth Science b) procedure Eng. Please bring a copy of your Abstract & Certification to the fair. obtained including the final clearance of the Intel ISEF Scientific Review Committee. This abstract describes only procedures performed by me/us. you are required to write Title Finalist’s Name Pick one only-- mark an “X” in a (maximum) 250 word.: Electrical & Mechanical d) conclusions Energy & Transportation Environmental It may also include any possible research Sciences applications. or interacted with (check all working). please refer to instructions from your affiliated fair. yes no 3. an Abstract & Certification is required at the conclusion of research. Page 28 International Rules for Precollege Science Research: Guidelines for Science and Engineering Fairs / 2009-2010 . My display board includes non-published photographs/visual depictions At the bottom of the Abstract & Certification of humans (other than myself): yes no form there are six questions. Revisions or questions will be resolved via an SRC appointment on site at the Intel ISEF. Details on this requirement follow. No other format or version of your approved Abstract & Certification will be allowed for any purpose at the Intel ISEF. Only minimal reference to Environmental Management previous work may be included. Information on Required Abstract & Certification for ALL Projects at the Intel ISEF * This form may not be relevant for your regional or state fair.* In ADDITION to the basic form requirements for ALL Projects and any other requirements due to specific areas of research. reflects my/our mentor own independent research. or self-promotions and that apply): human subjects potentially hazardous biological agents: external endorsements vertebrate animals microorganisms rDNA tissue b) work or procedures done by the 2. yes no ISEF OFFICIAL USE ONLY The Intel ISEF Scientific Research Committee will review and approve the abstract and This embossed seal attests that this project is in compliance with all federal and state laws and regulations and that all appropriate reviews and approvals have been answers to the questions. Completing the Abstract After finishing research and Intel ISEF Sample Abstract & Certification Category experimentation. City and State. No pasted or taped text will be permitted. Only after final Intel ISEF SRC approval has been obtained via a stamped/embossed copy of this Abstract & NOTE: Your abstract must be on the Intel International Science Certification may a Finalist make copies to and Engineering Fair Abstract & Certification form and embossed/ hand out to the judges and the public. the student directly handled. This project is a continuation of previous research. are correct and properly reflect my/our own work. yes no Completing the Certification 5. Country box at right This should be written on the Official Animal Sciences Abstract and Certification Form as Start Typing the Body of Your Abstract Here Beginning at the Left Margin Behavioral and Social Science provided by Society for Science & the Biochemistry Public. As a part of this research project. and represents one year’s work only. Materials & Bioengineering c) data Eng. stamped by the Intel ISEF Scientific Review Committee before it is displayed or handed out. Please read 6. I/We worked or used equipment in a regulated research institution or industrial setting. yes no 4. I/We hereby certify that the abstract and responses to the above statements FOR INTEL each carefully and answer appropriately. one-page abstract. An Mathematical Sciences Medicine and Health abstract must not include the following: Microbiology a) acknowledgments (including Physics & Astronomy Plant Sciences naming the research institution and/ or mentor with which you were 1.

17-20 for full text of the rules) Vertebrate Animal Form (5A) .) Potentially Hazardous Biological Agents Risk Assessment Form (6A) Human and Vertebrate Animal Tissue Form (6B) . 21-24 for full text of the rules. 13-16 for full text of the rules) Human Subjects Form (4) Qualified Scientist Form (2) (when applicable and/or required by the IRB) Vertebrate Animals (Requires prior approval. see pp. Qualified Scientist Form (2) (when applicable) Risk Assessment Form (3) Required for projects involving protists. blood. see pp. fuel production or other non-culturing experiments (6A. 2) I have reviewed the student’s completed Student Checklist (1A) and Research Plan. IACUC or IBC: Humans Potentially Hazardous Biological Agents: Vertebrate Animals Microorganisms rDNA Tissues 5) Forms to be completed for ALL Projects: Adult Sponsor Checklist (1) Research Plan Student Checklist (1A) Approval Form (1B) Regulated Research Institutional/Industrial Setting Form (1C) (when applicable) Continuation Form (7) (when applicable) 6) Additional forms required if the project includes the use of one or more of the following (check all that apply): Humans (Requires prior approval by an Institutional Review Board (IRB).to be completed in addition to Form 6A when project involves the use of fresh or frozen tissue. (Institutional Animal Care and Use Committee (IACUC) approval required prior experimentation. 3) I have worked with the student and we have discussed the possible risks involved in the project.for projects conducted in a non-regulated research site (SRC prior approval required. blood products and body fluids.org/isef Page 29 .) Risk Assessment Form (3) Qualified Scientist Form (2) (required for projects involving DEA-controlled substances or when applicable) Adult Sponsor’s Printed Name Signature Date of Review (Must be prior to experimentation. 6B and 2 are not required) Hazardous Chemicals.) Vertebrate Animal Form (5B) . primary cell cultures. Checklist for Adult Sponsor (1) This completed form is required for ALL projects and must be completed before experimentation To be completed by the Adult Sponsor in collaboration with the student researcher: Student’s Name: Project Title: 1) I have reviewed the ISEF Rules and Guidelines. 4) The project involves one or more of the following and requires prior approval by an SRC. IRB. archae and similar microorganisms and for projects using manure for composting.) Phone Email International Rules 2009/2010 full text of the rules and copies of forms are available at www.25-27 for full text of the rules. see pp. IACUC or Institutional Biosafety Committee (IBC).for projects conducted at a Regulated Research Institution. see pp. Activities and Devices (No prior approval required.) Qualified Scientist Form (2) (Required for all vertebrate animal projects at a regulated research site or when applicable) Potentially Hazardous Biological Agents (Requires prior approval by SRC.societyforscience.

1) a.org/isef Page 30 . Team Member: c.societyforscience. International Rules 2009/2010 full text of the rules and copies of forms are available at www. Student/Team Leader: Grade: Email: Phone: b. Student Checklist (1A) This form is required for ALL projects. 10) An abstract is required for all projects after experimentation (see page 28). Team Member: 2) Title of Project: 3) School: School Phone: School Address: 4) Adult Sponsor: Phone/Email: 5) Is this a continuation from a previous year? Yes No If Yes: a) Attach the previous year’s Abstract Form 1A and Research Plan b) Explain how this project is new and different from previous years on Continuation Form (7) 6) This year’s laboratory experiment/data collection will begin: (must be stated (mm/dd/yy) Projected Start Date: Projected End Date: (Projected dates are required for projects that require SRC/IRB prior review) ACTUAL Start Date: ACTUAL End Date: 7) Where will you conduct your experimentation? (check all that apply) Research Institution School Field Home Other: _________________________ 8) List name and address of all non-school work site(s): Name: Address: Phone: 9) Complete a Research Plan as described on page 31 and attach to this form.

one of these references must be an animal care reference. Activities & Devices (See instructions on p. prisoners.25 of the International Rules): • Describe Risk Assessment process and results • Detail chemical concentrations and drug dosages • Describe safety precautions and procedures to minimize risk • Discuss methods of disposal International Rules 2009/2010 full text of the rules and copies of forms are available at www. o Include justification of the numbers planned for the research • Describe housing and oversight of daily care • Discuss disposition of the animals at the termination of the study 3.21 of the International Rules): • Describe Biosafety Level Assessment process and resultant BSL determination • Give source of agent. legal etc) to participants? How will you minimize the risks? • Benefits. sex. names. Hazardous Chemicals. Describe who will participate in your study (age range. science journal articles. Vertebrate animal research (See instructions on p. Description in detail of method or procedures (The following are important and key items that should be included when formulating ANY AND ALL research plans. • Recruitment. Will any identifiable information (e. If not anonymous. Human subjects research (See instructions on p. Research Plan Instructions A complete research plan is required and must accompany Checklist for Student (1A) Provide a typed research plan and attach to Student Checklist (1A). List any benefits to society or each participant. Hypothesis/Engineering Goals C. Identify any vulnerable populations (minors.17 of the International Rules): • Briefly discuss POTENTIAL ALTERNATIVES and present a detailed justification for use of vertebrate animals • Explain potential impact or contribution this research may have • Detail all procedures to be used o Include methods used to minimize potential discomfort. If you plan to use vertebrate animals. that their participation is voluntary and they have the right to stop at any time. 2.g. books. 13 of the International Rules): • Subjects. gender. age. what they will be asked to do. birthdates. Potentially Hazardous Biological Agents (See instructions on p.org/isef Page 31 . What are the risks or potential discomforts (physical. distress. social. racial/ethnic composition). Question or Problem being addressed B. telephone numbers. pain and injury to the animals during the course of experimentation o Detailed chemical concentrations and drug dosages • Detail animal numbers. time involved. Bibliography: List at least five (5) major references (e.societyforscience. species. what procedures are in place for safeguarding confidentiality? Where will the data be stored? Who will have access to the data? What will you do with the data at the end of the study? • Informed Consent Process. What will participants be asked to do? Will you use any surveys. source of specific cell line.) • Procedures: Detail all procedures and experimental design to be used for data collection • Data Analysis: Describe the procedures you will use to analyze the data that answer research question or hypothesis D. email addresses) be collected? Will data be confidential or anonymous? If anonymous. The research plan for ALL projects is to include the following: A. • Protection of Privacy. describe how the data will be collected anony- mously.. questionnaires or tests? What is the frequency and length of time involved for each subject? • Risks. mentally disabled or economically disadvantaged). Describe how you will inform participants about the purpose of the study. etc. internet sites) from your literature review.g. • Detail safety precautions • Discuss methods of disposal 4. o Choose one style and use it consistently to reference the literature used in the research plan o Guidelines can be found in the Student Handbook Items 1-4 below are guidelines to be followed when applicable: 1. strain. etc. Where will you find your subjects? How will they be invited to participate? • Methods. pregnant women. psychological.

) 3) Final ISEF Affiliated Fair SRC Approval (Required for ALL Projects) SRC Approval After Experimentation and Shortly Before Competition at Regional/State/National Fair I certify that this project adheres to the approved Research Plan and complies with all ISEF Rules. IACUC.). Parent/Guardian’s Printed Name Signature Date of Approval (Must be prior to experimentation.g. was reviewed Plan and all the required forms are included. Such practices include plagiarism. (1C) and required institutional approvals (e. Fraudulent projects will fail to qualify for competition in affiliated fairs or the ISEF. Approval Form (1B) A completed form is required for each student. Attach begins experimentation. etc. My signature and approved by the proper institutional board before indicates approval of the Research Plan before the student experimentation and complies with the ISEF Rules.) 2) To be completed by the Fair SRC (Required for projects requiring prior SRC/IRB APPROVAL.) b) Parent/Guardian Approval: I have read and understand the risks and possible dangers involved in the Research Plan. Student’s Printed Name Signature Date Acknowledged (Must be prior to experimentation. use or presentation of other researcher’s work as one’s own.societyforscience. including all team members. IRB) SRC/IRB Chair’s Printed Name SRC Chair’s Printed Name Signature Date of Approval Signature Date of Approval (Must be prior to experimentation. agents) OR This project was conducted at a regulated research The SRC/IRB has carefully studied this project’s Research institution (not home or high school. Regional SRC Chair’s Printed Name Signature Date of Approval State/National SRC Chair’s Printed Name Signature Date of Approval (where applicable) International Rules 2009/2010 full text of the rules and copies of forms are available at www.) a) Required for projects that need prior SRC/ b) Required for research conducted at all IRB approval BEFORE experimentation Regulated Research Institutions with no (humans. • : I have read and will abide by the following Ethics statement Scientific fraud and misconduct are not condoned at any level of research or competition. vertebrates or potentially hazardous biological prior fair SRC/IRB approval. Sign 2a or 2b as appropriate. forgery.org/isef Page 32 . I consent to my child participating in this research. 1) To Be Completed by Student and Parent a) Student Acknowledgment: • I understand the risks and possible dangers to me of the proposed research plan. and fabrication of data. • I have read the ISEF Rules and Guidelines and will adhere to all International Rules when conducting this research.

school or field. etc. how large was the group and what kind of research group was it (students.) The student conducted research at my work site: a) to use the equipment b) to perform experiment(s)/conduct research 1) How did the student get the idea for her/his project? (e. This form MUST be displayed with your project. (Do not list procedures student only observed. Regulated Research Institutional/Industrial Setting Form (1C) This form must be completed after experimentation by the adult supervising the student research conducted in a regulated research institution. Copy of approval(s) must be attached. Was the project assigned. picked from a list. if applicable.) 4) What specific procedures or equipment did the student actually use for the project. Please list and describe. vertebrate animals or potentially hazardous biological agents require review and approval by an institutional regulatory board (IRB/IACUC/IBC).org/isef Page 33 . Supervising Adult’s Printed Name Signature Title Institution Date Signed Address Email/ Phone International Rules 2009/2010 full text of the rules and copies of forms are available at www. Responses must be on the form Student’s Name Title of Project To be completed by the Supervising Adult in the Setting (NOT the Student) after experimentation: (Responses must remain on the form as it is required to be displayed at student’s project booth.g.) 2) Have you reviewed the ISEF rules relevant to this project? Yes No 3) Did the student work on the project as a part of a research group? Yes No If yes. group of adult researchers. etc.) 5) How independent or creative was the student’s work? Student research projects dealing with human subjects. industrial setting or any work site other than home. an original student idea.societyforscience.

. . . . . . . . If no. Designated Supervisor’s Printed Name Qualified Scientist’s Printed Name Signature Date of Approval Signature Date of Approval Phone Email International Rules 2009/2010 full text of the rules and copies of forms are available at www. . . . . and I will be used by the student in the Research Plan. . . . . . . . . . . . I will ensure her/his training. . . . . . . . . including blood and blood products) . . . . . . . . . . yes no a. . . . . . who will directly supervise and serve as the Designated Supervisor? b. . . . . . . . . . . . . . vertebrate animals. . . . . yes no b) Vertebrate animals . . . . . . . . . I understand that a provide direct supervision. . . . . . . . . . . . . . . . . . . . . . . . . I have a working knowledge of the techniques to trained in the techniques to be used by this student. .org/isef Page 34 . . . potentially hazardous biological agents. . . . . . . . . yes no d) DEA-controlled substances . . . Qualified Scientist Form (2) May be required for research involving human subjects. Student’s Name Title of Project To be completed by the Qualified Scientist: Scientist Name: Educational Background: Degree(s): Experience/Training as relates to the student’s area of research: Position: Institution: Address: Email/Phone: 1) Have you reviewed the ISEF rules relevant to this project? yes no 2) Will any of the following be used? a) Human subjects . . . . . . . . . . rDNA and tissues. . . . . . . . . Experience/Training of the Designated Supervisor: 4) Describe the safety precautions and training necessary for this project: To be completed by the Qualified Scientist: To be completed by the Designated Supervisor I certify that I have reviewed and approved the Research Plan when the Qualified Scientist cannot directly prior to the start of the experimentation. yes no 3) Will you directly supervise the student? . Designated Supervisor is not trained in the necessary procedures. . . . . . . . . . . . . . . . . . . . . . yes no c) Potentially hazardous biological agents (microorganisms. . Must be completed and signed before the start of student experimentation. . Designated Supervisor is required when the student is not conducting experimentation under my direct supervision.societyforscience. . . . and DEA-controlled substances. . . . . . . . I will provide advice and supervision I certify that I have reviewed the Research Plan and have been during the research. . . . . . . . . . If the student or supervise. . . . . .

I certify that I have reviewed the Research Plan and will provide direct supervision. Identify and assess the risks involved. Describe the safety precautions and procedures that will be used to reduce the risks. Describe the disposal procedures that will be used (when applicable). activities or devices.societyforscience.org/isef Page 35 . Student’s Name Title of Project To be completed by the Student Researcher in collaboration with Designated Supervisor/Qualified Scientist: (All questions must be answered. Designated Supervisor’s Printed Name Signature Date of Review (must be prior to experimentation.) Position & Institution Phone or email contact information Experience/Training as relates to the student’s area of research International Rules 2009/2010 full text of the rules and copies of forms are available at www. 4.) 1. 3. additional page(s) may be attached. 5. devices or microorganisms that will be used. Risk Assessment Form (3) Required for projects using hazardous chemicals. Must be completed before experimentation. List the source(s) of safety information. To be completed and signed by the Designated Supervisor (or Qualified Scientist. 2. List/identify the hazardous chemicals. when applicable): I agree with the risk assessment and safety precautions and procedures described above. activities.

5. medical doctor.) Student’s Name Title of Project Adult Sponsor: Contact Phone/Email: To be completed by Student Researcher in collaboration with the Adult Sponsor/Designated Supervisor/Qualified Scientist: 1. If you answered NO to questions 3 or 4 (no waiver requested). 2. qualified scientist or related to (e. Risk Level (check one) : Minimal Risk More than Minimal Risk 2. (IRB approval required before experimentation. licensed clinical professional counselor. mother. Written Parental Permission required for minor subjects: Yes No Not applicable (No minors in this study) 5.org/isef Page 36 . father of) the student (conflict of interest). or registered nurse) Printed Name Degree Signature Date of Approval School Administrator Printed Name Degree Signature Date of Approval Educator Printed Name Degree Signature Date of Approval International Rules 2009/2010 full text of the rules and copies of forms are available at www. Written Minor Assent required for minor subjects: Yes No Not applicable (No minors in this study) 4.g.societyforscience. I have attached any surveys or questionnaires I will be using in my project. Qualified Scientist (QS) Required: Yes No 3. Yes No Are you working with a Qualified Scientist? Name: Degree: Email Address/Phone Number: Experience/Training as it relates to this project: To be completed by Institutional Review Board (IRB) after review of the research plan. Yes No Not Applicable I am requesting a waiver for obtaining parental permission. licensed social worker.. 3. Check one of the following: Research project requires revisions and is NOT approved at this time. attach the consent form you will use. I have submitted my Research Plan which addresses ALL areas indicated in the Human Subjects Section of the Research Plan Instructions. 4. psychiatrist. I attest that I have reviewed the student’s project and agree with the above IRB determinations. The submitted Research Plan must address all areas indicated on the Human Subjects section of the Research Plan Instructions. physician’s assistant. Yes No I am requesting a waiver of the documentation of informed consent and/or minor assent. Research project is Approved with the following conditions below: (All 5 must be answered) 1. Human Subjects Form (4) Required for all research involving human subjects. Written Informed Consent required for subjects 18 years or older: Yes No Not applicable (No subjects 18 yrs or older in this study) IRB SIGNATURES (All 3 signatures required) None of these individuals may be the adult sponsor. designated supervisor. Medical or Mental Health Professional (a psychologist. IRB will attach document indicating concerns and/or requested revisions.

the researcher keeps the original.org/isef Page 37 . If you would like to participate. Sample of Informed Consent Form Instructions to the Student Researcher: An informed consent form should be developed in consultation with the Adult Sponsor. Please read the following information about the project. Purpose of the project: If you participate. • Students may use this form or may copy ALL elements of this form into a new document. I am asking for your voluntary participation in my science fair project. If you decide not to participate there will not be any negative consequences. Designated Supervisor or Qualified Scientist. please sign in the appropriate box below. you will be asked to: Time required for participation: Risks: Benefits: How confidentiality will be maintained: If you have any questions about this study. By signing this form I am attesting that I have read and understand the information above and I freely give my consent/ assent to participate or permission for my child to participate. signed form. • When written documentation is required. you may stop participating at any time and you may decide not to answer any specific question. Please be aware that if you decide to participate.societyforscience. Adult Informed Consent or Minor Assent Date Reviewed & Signed: Printed Name of Research Subject: Signature: Parental/Guardian Permission (if applicable) Date Reviewed & Signed: Parent/Guardian Printed Name: Signature: International Rules 2009/2010 full text of the rules and copies of forms are available at www. minor assent and/or parental permission. feel free to contact: Adult Sponsor: __________________________ Phone/email: ______________________________________ Voluntary Participation: Participation in this study is completely voluntary. This form is used to provide information to the research subject (or parent/guardian) and to document written informed consent.

frequency of food and water. experimentation and I accept primary responsibility for I certify that I have approved the use and dosages of the care and handling of the animals in this project.org/isef Page 38 . Veterinarian and Designated Supervisor REQUIRED.) Student’s Name Title of Project To be completed by Student Researcher: 1. Printed Name Email/Phone Printed Name Email/Phone Signature Date of Approval Signature Date of Approval International Rules 2009/2010 full text of the rules and copies of forms are available at www. Designated Supervisor and Qualified Scientist REQUIRED. What will happen to the animals after experimentation? To be completed by Scientific Review Committee (SRC) BEFORE experimentation Level of Supervision Required for agricultural.societyforscience. I certify that I will directly supervise the experiment. number of animals per cage. type of food. etc. species) and number of animals used. Veterinarian. I certify that I will provide veterinary medical and nursing care in case of illness or emergency. 2. bedding. (SRC approval required before experimentation. Common name (or Genus. environment. prescription drugs and/or nutritional supplements. The SRC has carefully reviewed this study and finds it is an appropriate study that may be conducted in a non-regulated research site. Include the cage/pen size. behavioral or nutritional studies: Designated Supervisor REQUIRED. Please have applicable person sign below. SRC Pre-Approval Signature: SRC Chair Printed Name Signature Date of Approval To be completed by Veterinarian: To be completed by Designated Supervisor: I certify that I have reviewed this research and animal I certify that I have reviewed this research and animal husbandry with the student before the start of husbandry with the student before the start of experimentation. 3. Please have applicable persons sign below and have the Qualified Scientist complete Form (2). Please have applicable persons sign below. Describe completely the housing and husbandry to be provided. how often animal is observed. Vertebrate Animal Form (5A) Required for all research involving vertebrate animals that is conducted in a Non-Regulated Research Site.

the role of the student in this project: procedures and equipment they were involved with. was provided to the student? 4. Have you reviewed the ISEF Rules relevant to this project? 3. (IACUC approval required before experimentation. Certification or Documentation of Student Researcher Training List Certificate Number or Attach Documentation Date(s) of Training Qualified Scientist/Principal Investigator Printed Name Signature Date IACUC Chair/Coordinator Printed Name Signature Date International Rules 2009/2010 full text of the rules and copies of forms are available at www. Describe.) Student’s Name Title of Project Title and Protocol Number of IACUC Approved Project To be completed by Qualified Scientist or Principal Investigator: 1. including dates. What laboratory training. oversight provided and safety precautions employed. in detail.societyforscience. Was this a student-generated idea or was it a subset of your work? 2. A letter from the Qualified Scientist or Principal Investigator is not sufficient.org/isef Page 39 . USDA Pain Category designated for this study: 6.) 7. Vertebrate Animal Form (5B) Required for all research involving vertebrate animals that is conducted at a Regulated Research Institution. (Attach extra pages if necessary. Species of animals used: Number of animals used: 5. Attach a copy of the Regulated Research Institution IACUC Approval.

QS/DS Printed Name Signature Date of Signature Experience/training of Designated Supervisor as it relates to the student’s area of research (if applicable) To be completed by SRC prior to experimentation: To be completed by SRC after experimentation with Institutional pre-approval: The SRC has carefully studied this project’s This project was reviewed and approved by the Research Plan and the risk level assessment above appropriate institutional board (e. 2) Describe the site of experimentation including the level of biological containment.) 1) Identify potentially hazardous biological agents to be used in this experiment. SRC/IACUC/IBC approval required before experimentation. IACUC. fresh/frozen tissue.g. SRC Chair’s Printed Name SRC Chair’s Printed Name Signature Date of Approval Signature Date of Approval International Rules 2009/2010 full text of the rules and copies of forms are available at www. blood and body fluids. 3) Describe the method of disposal of all cultured materials and other potentially hazardous biological agents.) 5) What final biosafety level do you recommend for this project given the risk assessment you conducted? To be completed by Qualified Scientist or Designated Supervisor 1) What training will the student receive for this project? 2) Do you concur with the biosafety information and recommendation provided by the student researcher above? Yes No If no. IBC) and approves this study as a BSL-1 study. The institution does not require approval for this which must be conducted at a BSL-2 or above type of study.societyforscience.org/isef Page 40 . hood type. additional page(s) may be attached. Include the source. rDNA. (personal protective equip. The The SRC has carefully studied this project’s required institutional forms are attached. please explain. laboratory and complies with the ISEF rules. training.. The student has received proper laboratory. etc. 4) Describe the procedures that will be used to minimize risk. Student’s Name Title of Project To be completed by Student Researcher in collaboration with Qualified Scientist/Designated Supervisor: (All questions are applicable and must be answered. Potentially Hazardous Biological Agents Risk Assessment Form (6A) Required for research involving microorganisms. Attached is a letter from an institutional representative certifying the above. which before experimentation at a BSL-1 or BSL-2 must be conducted at a BSL-1 or above laboratory. quantity and the biosafety level risk group of each microorganism. Research Plan and the risk level assessment above and approves this study as a BSL-2 study.

blood products and body fluids. blood. the IACUC approval number and date of IACUC approval. cultures or cells that will be supplied to him/her by myself or qualified personnel from the laboratory. tissues or body fluids in this project will be handled in accordance with the standards and guidance set forth in Occupational Safety and Health Act. 29CFR. must also complete Form 6A. blood products.societyforscience. tissues. Subpart Z.org/isef Page 41 . organ(s). primary cell cultures. Student’s Name Title of Project To be completed by Student Researcher: 1) What tissue(s). please provide information regarding the vertebrate study from which the tissue was obtained. 1910. Printed Name Signature Date Signed (Must be prior to experimentation. If the research involves living organisms. please ensure that the proper human or animal forms are completed. or part(s) will be used? 2) Where will the above tissue.) Title Phone/Email Institution International Rules 2009/2010 full text of the rules and copies of forms are available at www.1030 - Blood Borne Pathogens. organ. or part be obtained (identify each separately): 3) If the tissue is obtained from a source within a research institution. and that if vertebrate animals were euthanized they were euthanized for a purpose other than the student’s research. Attach a copy of the IACUC certification with the name of the research institution. To be completed by the Qualified Scientist or Designated Supervisor: I verify that the student will work solely with organs. AND/OR I certify that the blood. All projects using any tissue listed above. the title of the study. Human and Vertebrate Animal Tissue Form (6B) Required for projects using fresh/frozen tissue.

This form must be accompanied by the previous year’s abstract and Research Plan. Continuation Projects Form (7) Required for projects that are a continuation in the same field of study as a previous project. The information must be on the form. Objectives 2007-2008: 2008-2009: 4. use an additional form for 2006 and earlier projects. Components Current Research Project Previous Research Project 1. Line of 2008-2009: investigation/ central theme 2007-2008: of research 2008-2009:: 3. Student’s Name To be completed by Student Researcher: List all components of the current project that make it new and different from previous research. Title 2008-2009: 2007-2008: 2.societyforscience. Additional changes 2007-2008: Attached are: 2009 Abstract and Research Plan 2008 Abstract 2007 Abstract I hereby certify that the above information is correct and that the current year Abstract & Certification and project display board properly reflect work done only in the current year. Variables studied 2007-2008: 2008-2009: 5. Student’s Printed Name Signature Date of Signature International Rules 2009/2010 full text of the rules and copies of forms are available at www.org/isef Page 42 .

Science News.societyforscience. helping to change policy. www. Intel Corporation Society for Science & the Public The foundation of tomorrow’s innovation is education. *Other names and brands may be claimed as the property of others.org/isef © 2009 Society for Science & the Public. young innovators.785. and technology access to help tomorrow’s Fair.org In the last decade. dedicated to public engagement in science the world—with the help of technology—has inspired Intel’s and science education. 20036-2801 202. SSP is a leading commitment to education for 40 years. educate. All rights reserved. D. All rights reserved. We do more than make advocate for the understanding and appreciation of science and contributions. www. Printed in USA 0309/JAF/LAI/XX/XX Please Recycle . and other countries. Intel gets directly involved in developing and the vital role it plays in human advancement. toward improving education in 50 countries. visit innovators. Established in 1921. SSP is committed to inform. training teachers.S. That’s Society for Science & the Public (SSP) is one of the oldest nonprofit why making quality education available to more students around organizations in the U.intel. www. © 2009 Intel Corporation.org/isef The Intel International Science and Engineering Fair and Intel Science Talent Search encourage students to tackle challenging scientific questions and develop the skills needed to solve the problems of tomorrow.societyforscience.S. Through its acclaimed education competitions and its award-winning providing kids with a place to explore technology. Intel believes that students at all levels and inspire. Intel has invested more than $1 billion.2255 telephone 202. We are actively involved in education To learn more about the Intel International Science and Engineering programs.1243 fax societyforscience.C. advocacy.com/education Society for Science & the Public 1719 N Street. NW Washington.785. offering free curricula. everywhere deserve to have the skills they need to become part of the next generation of innovators. and Intel employees have donated over 2 million hours. Intel and the Intel logo are trademarks of Intel Corporation in the U. and encouraging magazine.