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N.S.

SRIKANTH
C/o Mr. Venu Gopal Machiraju,
22nd link street, 3rd main road,
IndiraNagar, Adyar, 9940274053
Chennai 600020 srikanth.nuka@gmail.com

Career objective

To pursue a challenging and rewarding career where I can use my technical
and analytical skills.

Qualifications

Course Institute University Percentage Year
M.Sc. P. R. College Final result
Bharathidasan
Biochemistry (accredited with awaiting, 70% 2005-2007
University
NAAC`A'Grade) till III semister
B.Sc.
Acharya
(Botany, Andhra Loyola
Nagarjuna 80.3% 2002-2005
Zoology, College
University
Chemistry)
Diploma in
Andhra Loyola
Medical Lab Autonomous 75.0% 2002-2003
College
Technology
Andhra Loyola Board of
Intermediate 75.3% 2000-2002
College Intermediate
Board of
S. T. B. E. M.
SSC Secondary 65.0% 1999-2000
High School
Education

Training undergone

Title : Training on techniques in Stem Cell Technology & Molecular Biology.
Location: University of Madras, Guindy campus
Duration: June to July 2006

Educational Projects:

M.Sc. Project: CANCER STEM CELLS
Title : Differential expression of CD34 and NOTCH in the normal and
cancerous tissues of gastric epithelium and its implication in
carcinogenesis.
Location : University of Madras, Guindy campus.
Duration : June to August 2007
Guide : Dr.H.Devaraj, Professor of Biochemistry & Director, Guindy
Campus, University of Madras.
Description :
Ectopic expression of CD 34 observed in gastric cancer not in normal gastric
cells by RT-PCR indicates the presence of CD 34 expressing undifferentiated HSCs
in the cancerous mass. NOTCH suppresses differentiation of HSCs and promotes
retention of cancer stem cell properties, so its reexpression during the gastric
neoplastic transformation favors cancerous growth. From our experiment, it has
been observed that the retention of dedifferentiated characteristic of CD34 expressing
HSC cell precursors created by notch expression could promote tumor progression
under proliferative environment created by chronic inflammation and their mediators.

B.Sc. Project: PLANT BIOTECHNOLOGY AND CULTURE
Title : In vitro fruiting of Datura inoxia using Ovary Culture
Location : Andhra Loyola College, Acharya Nagarjuna University.
Duration : November 2004 to March 2005.
Guide : Fr.Dr.S.Emmanuel.sj.
Description :
Evaluating the effect of plant growth hormones and effect of different media
on in vitro fruiting from ovaries. Experiments were conducted with MS medium,
Gamborg, B5 medium with different growth hormones like BAP, kinetin, GA, NAA
at different concentrations. Results obtained concluded that BAP is suitable hormone
to obtain in vitro fruiting on full strength MS medium.

Exposure to Methodology/Techniques/Instruments
1. INSTRUMENTATION: Agarose gel Electrophoresis, SDS-PAGE, Western
Blotting, Paper Chromatography.
2. TECHNIQUES: Isolation of genomic DNA, Isolation & Quantification of
RNA, RT- PCR, Gradient-PCR, Immunohistochemistry, Isolation &
Estimation of Protein.

Seminars & Technical paper presentations

 Presented on Effect of Pesticides on Aquaculture in “Loyola vision 2000
plus”, a science exhibition in 2000.
 Presented on Electricity through bio sources in “Golden jubilee science and
arts exhibition” in 2004.
 Presented a paper on Cirrhosis, a chronic liver disease in “Emerging trends in
life sciences 2006” a UGC sponsored National Level Seminar in 2006.
 Participated in International Level Seminar “Life through chemistry” in 2006.

Computer Skills & Knowledge

Familiar with computer fundamentals, packages like Ms Office.

Extra Curricular Activities
Active participant in college activities,
1. Participated in skit competition in “Sphoorti literary and cultural
competitions” in 2005.
2. Participated actively in NSS programmes and camps during 2002-2005.

Personal Details

1. Fathers name : N. Madhusudana Rao
2. Date of Birth : 07/09/1984
3. Marital status : Single
4. Languages Known : English, Hindi, Telugu and Tamil.