You are on page 1of 3

CHOLESTEROL

Cholesterol is sterol of the body. It is present in the bile as solid alcohol form.
It occurs as a white or faint yellow in color.
SOURCES:
1. Eo!enous" #iet" $utter Cream% &il'% E!! yol' (nimal Or!ans $rain )idney
Li*er Spleen
+. Endo!enous" In body it will be formed by (cetyl Co( en,yme
FUNCTIONS:
1- It is one of the structural component of the cell membrane
+- It is the precursor of $ile Salt% Steroid Hormones and .itamins #.
/- It is the constitute of plasma lipoprotein.
Normal value 101 2 +01 m! 3 dl
Clinical Interpretation

(A) HYPERCHOESTEROE!IA:
Increased le*el of cholesterol in blood is called Hypercholesterolemia and the cause are"
1- 4ephrotic Syndrome
+- Obstructi*e 5aundice
/- #iabetic &ellitus
6- (therosclerosis
0- Increased endo!enous formation of cholesterol
7- Increased inta'e of Saturated 8ree .8atty (cids
9- La'e of Eercise
(") HYPOCHOESTEROE!IA:
It is decreased cholesterol le*el in blood causes are"
1- Hyperthyroidism :Thyrotoicosis- Hi!h &etabolism
+- (naemias
/- Star*ation :#eficiency in diet-
6- #ecrease Inta'e of Saturated 8ree 8atty (cids
0- Hea*y Eercise.
E;<ERI&E4T
O"#ECT :
TO $ETER!INE THE CHOESTERO FRO! THE %I&EN SA!PE OF
"OO$'
!et(o):
There are two methods.
1- Liebermann $urchard
+- En,ymatic &ethod
PRINCIPE:
The cholesterol is determined after en,ymatic hydrolysis and oidation the indicator
=uinoneimine is formed from hydro!en peroide and 6>aminophena,one in the
presence of phenol and peroidase.
RE*UIRE!ENT:
1- En+,matic Solution" containin! phosphate buffer% 6 2 amino phena,one%
phenol% peroides% cholesterol esterase% cholesterol oidase and sodiuma,ide.
+- Stan)ar) -olution: containin! cholesterol " at the conc" of +11 m! 3 dl.

/- Specimen serum of heparina,ide or E#T( contain blood.
SA!PE OF THE "OO$
Ta'e + cc of blood from *ein? centrifu!e it for few minutes to !et serum
Proce)ure:
Ta'e three test tubes and label them as T and S and $ respecti*ely and
pipette into them as below
Solution Test Standard $lan'
En,ymatic
Solution
+ml +ml +ml
Standard >>>>>>>>>> 1.1+ml >>>>>>>>>
Serum 1.1+ ml >>>>>>>> >>>>>>>>>>
4ow mi well the test tube and incubate them for 11 minutes at +0
1
C or 0 minutes
at /9
1
C &easure the absorbance of sample and standard a!ainst blan' within 71
minutes under wa*e len!th of 011 to 067 nm
Calculation:
Readin! of sample ; +11 >>>>> m! 3 dl
Readin! of Standard
Re-ult:
The !i*en sample of blood contain >>>>>>>>>>>m! 3 dl of cholesterol
4ormal *alue 101 >+01 m! 3 dl