APPLICATION FORM FOR KHORANA SUMMER RESEARCH

Khorana Scholars Program 2010

1. Name:
First name Sri Middle name balaji

Surname Ponraj

2. Male/Female: Male

3. Date of Birth: 01/09/1986

4. Passport number: g5792123

5. Name of the University: Anna University Coimbatore

6. Address:

Anna University Coimbatore, Academic Campus

University Address Jothipuram, Coimbatore - 641 047
East Street, Anaimalaianpatty, Uthamapalayam(Taluk),
Residential Address Theni(District), Tamil nadu(State), India 625 526

Landline Number # Mobile #

Telephone 04222696341 9894844829

E-mail bathani.rajbt@gmail.com

Skype id bathani01

7. Major field of study: Nanotechnology

8. Degree program currently enrolled in: Master of Technology

9. Cumulative Grade Point Average: 9.16 (First year)

10. Expected date of Graduation : June 2010

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 11. Academic Record:

EDUCATIONAL QUALIFICATIONS (10th Standard onwards)

Percent-
Duration
Examination/ University/ Institute/ Subjects studied/ age/
(from mm/yy to
Degree Board College/ School Specialization CGPA/
mm/yy)
Rank
Science,
Vivekananda June 2001
Mathematics,
10th Standard Matriculation Matriculation to 84.45
History and
School March 2002
Geography
Theni Kammavar
Sangam June 2003 Physics, Chemistry,
HSCC State Board Matriculation to March Biology and 91.25
higher Secondary 2004 Mathematics
School
Arulmigu
August 2004
Anna University kalasalingam
B.Tech to Biotechnology 78.77
Chennai College of
April 2008
Engineering
August 2008
Anna University Anna University
M.Tech to June Nanotechnology 9.16
Coimbatore Coimbatore
2010

12. Research interests, possible project ideas and what you hope to gain from this
experience.
Research interests:
Title: 8-OHdG – a novel Biomarker to evaluate Oxidative DNA damage in Micro
vascular Complications.
Description: Vascular complications are the leading cause of morbidity and mortality in
patients with diabetes. In nuclear and mitochondrial DNA, 8-hydroxydeoxyguanosine (8-
OHdG), an oxidized nucleoside of DNA, is the most frequently detected and studied
DNA lesion. Upon DNA repair, 8-OHdG is excreted in the urine. Numerous studies have
shown that oxidative DNA damage links pathogenically to a variety of aging-associated
degenerative diseases such as cancer, coronary heart disease and diabetes. Quantification
of urinary 8-OHdG, a specific DNA repair products in the urine, can be made with a
simple ELISA. Finally, the study of Urinary 8-OHdG can lead to predict the earlier onset
of vascular complications.

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Project ideas:
Title: Synthesis and Characterization of Cerium oxide Nanoparticle to study the
hyperglycemia induced Endothelial Dysfunction in Cultured Bovine Retinal Endothelial
Cells.
Description: In order to combat the hyperglycemia induced Endothelial Dysfunction in
humans, I have proposed that rare-earth cerium oxide (CeO2) nanoparticles (free-radical
scavengers) protect normal tissue from hyperglycemia induced damage. Earlier studies
and reports suggest that these nanoparticles may be a therapeutic regenerative
nanomedicine that will scavenge reactive oxygen species, which are responsible for such
induced cell damage. In the end, CeO2 nanoparticles provide a tremendous potential for
endothelial cell protection and can lead to significant benefits for the preservation of
human health and the quality of life for humans having Diabetes Mellitus.

Gain from this experience:

1. Gain better understanding of the scientific process and the application of the
knowledge gained from basic science research and state of the art techniques to solve
complex problems in biology and medicine.
2. Like to be a member of a research community working at the forefront of science,
medicine and biotechnology.

13. Research experience, technical background, other internships, courses, lab

work, techniques exposed to and past lab experience.
Research experience:
Completed a project entitled “Induction of Apoptosis by Elevated levels of
Glucose through Caspase -3 Activation in Bovine Retinal Endothelial Cells” for a period
of four months at Animal Cell Culture Laboratory, Kalasalingam University, Tamil
Nadu, India.

Technical background:
Familiarized with the theoretical concepts and the practical applications of various
subjects like Bio-Nanotechnology, Nanolithography, Molecular Biology, Microbiology,
Animal Biotechnology, Plant Biotechnology and Immunology.

Internships:
Completed a project entitled “Strain improvement of Streptomyces.sp to
increase production of small molecules- Immunomycin” at Research and
Development Department, BIOCON LIMITED, Bangalore, India, from May 15th 2008
to August 16th 2008.

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Courses:
1. Undergone a training programme entitled “Maintenance of Industrially
important cells and their Molecular Characterization” organized by PSG
College of Technology Coimbatore and Supported by Department of
Biotechnology(Ministry of Science and Technology) from November 9th to
November 21st 2009.
2. Undergone an Inplant training programme at Quality Control and Production
Department, PHARMAFABRIKON Madurai, Tamil Nadu, India, from June
21st to June 27th 2006.
3. International Seminar and Workshop on Medical and Pharmaceutical
Nanotechnology held at Anna University Tiruchirappalli, Tamil Nadu, India
from September 25th to September 26th 2008.
4. National Workshop on MEMS in Biomedical Applications held at SSN College
of Engineering Chennai, Tamil Nadu, India from September 17th to September
18th 2009 .

Lab work:
PCR-RFLP, RAPD, Multiplex-PCR, Western Blotting, HPLC, Gel
Electrophoresis, Atomic Force microscopy, Scanning Tunneling Microscopy, Laser
Raman Spectro Photometer, Particle size Analyzer and Particle Spin coater.

Techniques exposed to and past lab experience:

1. High Performance Liquid Chromatography
Measured the activites of various Anti-Cancer molecules such as Tacrolimus,
Orlistat and Hygromycin through HPLC.

2. Cell Cytotoxocity Assays

The cells harvested from animal tissues and organs are analyzed for measuring
apoptosis. (Caspase and MTT Assay)

3. Maintenance of Industrially Important Cells and their Molecular

Characterization of Cells
Cryopreservation, Isolation of DNA, PCR-RFLP, RAPD, Multiplex PCR,
Western Blotting and PCR-Primer designing Bioinformatics tools.

4. Biochemical estimation on Type-II Diabetic patients

Biochemical estimation of Blood (Glucose, Creatinine, Urea and Hydrogen
Peroxide) and Urine (Glucose, Urea, Hydrogen Peroxide and Ketone bodies).

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16. Supporting Documentation check-list:

Current transcript (Latest Marks sheet) 

Two letters of recommendation from a

faculty member, advisor or lab supervisor on their official letterhead 

Statement from Parent Institution (in the format provided in S.No. 14)
