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Protein Discovery Inc.

Announces New FASP(TM) Protein

Digestion Kits

Mon Nov 22, 2010 8:01am EST

* Reuters is not responsible for the content in this press release.
KNOXVILLE, TN, Nov 22 (MARKET WIRE) -Protein Discovery today announced the introduction of two new sample
preparation kits designed to advance a "universal" sample preparation
protocol for mass spectrometry-based proteome research. Based on the
Filter-Aided Sample Preparation method, Protein Discovery's FASP Protein
Digestion Kits enable complete, reproducible solubilization and digestion
of both fluid and tissue sample proteomes, and extend mass
spectrometry-based proteome research methods into the previously
inaccessible realms of the insoluble and FFPE ("biobank") proteomes.
Comparative proteome studies, such as those required to develop clinical
diagnostics, have been impeded by mass spectrometry data variability
associated with incomplete proteome solubilization and incomplete
proteolytic digestion problems affecting conventional sample preparation
workflows. Protein Discovery's FASP Protein Digestion Kits eliminate this
source of variation by allowing researchers to completely solubilize the
proteomes of fluid and tissue samples with the use of aggressive
detergents, and to drive digestion reactions toward complete hydrolysis.
FASP Protein Digestion Kit
By enabling the use of strong detergents
within mass spectrometry sample preparation workflows, Protein

Discovery's new FASP Protein Digestion Kit offers a standardized sample

preparation workflow that works for both soluble and insoluble proteins
in fluids and tissues. Principal advantages of the FASP Protein Digestion
Kit include complete proteome solubilization, sample cleanup with
detergent depletion, and highly efficient proteolytic enzyme digestion of
FFPE-FASP Protein Digestion Kit
The composition of Protein Discovery's
new FFPE-FASP Protein Digestion Kit is similar to that of the FASP
Protein Digestion Kit, but the FFPE-FASP Kit contains supplemental
materials and protocol steps to guide researchers through additional
tissue sample and protein treatment steps. These steps are required to
ensure compatibility of FFPE (formalin-fixed paraffin-embedded) samples
with downstream mass spectrometry sample preparation and analysis
workflows. As the principal sample format of biobank libraries, FFPE
tissue samples represent a rich opportunity for productive proteome
research, as many of these samples are associated with specific clinical
histories and outcome measures. With the FFPE-FASP Protein Digestion Kit,
researchers will be able to study how the human proteome responds and
interacts with clinical events such as the initiation and discontinuation
of drug therapy, and disease stage progression.
"We agree with researchers who believe that the only way to achieve an
unbiased extraction and solubilize the entire proteome is to use a strong
detergent like SDS," said Jeremy Norris, PhD, vice president of research
and development at Protein Discovery. "Scientists can now apply the
productive mass spectrometry-based protein analysis methods developed

over the past fifteen years to the great expanse of the proteome that
isn't readily soluble in the weaker detergents they've had to use. And
now that the technique can also be used with biobank library tissues,
we'll be seeing more and more mass spectrometry studies with direct
clinical relevance," said Dr. Norris.
The FASP Protein Digestion Kit and the FFPE-FASP Protein Digestion Kit
are available exclusively from Protein Discovery, Inc. For pricing and
additional technical information, visit or
contact Protein Discovery by calling (865) 521-7400.
Protein Discovery develops and commercializes specialized sample
preparation products for mass spectrometry-based proteome research.
Headquarters are in Knoxville, Tennessee.
Chuck Witkowski
Protein Discovery, Inc.
tel 865-521-7400
toll free: 866-670-9038
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