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Lampiran 1

Perhitungan konsentrasi:
Konsentrasi 1 ppm

=

1000 mg didalam 1.000.000 ml akuades.

=

1 mg didalam 1.000 ml akuades.

Konsentrasi 1100 ppm

=

1100 mg / 1000 ml akuades.

Konsentrasi 1300 ppm

=

1300 mg / 1000 ml akuades.

Konsentrasi 1500 ppm

=

1500 mg / 1000 ml akuades.

Konsentrasi 1700 ppm

=

1700 mg / 1000 ml akuades.

Perhitungan temephos 1 ppm:
1 g abate = mengandung 0,01 g temephos = 10 mg temephos.
1 ppm larutan temephos = 1mg temephos / 1000 ml akuades.
Jadi bila ingin menghasilkan 1 ppm temephos dibutuhkan 1/10 g abate = 0,1 g abate.

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24 g.88 g.Lampiran 2 Perhitungan pengenceran: Dosis 1700 ppm = 170 mg / 100 ml CMC 1% Dosis 1500 ppm = 1500 / 1700 * 100 ml = 88 ml CMC 1% 12 ml Dosis 1300 ppm = 1300 / 1700 * 100 ml = 76 ml CMC 1% 24 ml Dosis 1100 ppm = 1100 / 1700 * 100 ml = 64 ml CMC 1%+ 36 ml+ 328 ml CMC1% 72 ml pengenceran • Total ekstrak etanol buah pare (EEBP) yang dibutuhkan: 1700 ppm + 1500 ppm + 1300 ppm + 1100 ppm = 5600 ppm = 560 mg * pengulangan (4) = 2240 mg = 2. • Total akuades yang dibutuhkan: 400 ml * pengulangan (4) = 1600 ml.72 g * pengulangan (4) = 2. • Total CMC 1% kering yang dibutuhkan untuk pengenceran: 0. • Total CMC 1% kering yang dibutuhkan untuk EEBP: 3. 54 .28 g * pengulangan (4) = 13.12 g.

Lampiran 3 Percobaan Trial and error 24 jam Konsentrasi Pengulangan 1 2 3 4 200 ppm Hidup Mati 30 0 30 0 30 0 30 0 300 ppm Hidup Mati 30 0 30 0 30 0 30 0 400 ppm Hidup Mati 30 0 30 0 30 0 30 0 Kontrol (-) 500 ppm Hidup Mati 30 0 30 0 30 0 30 0 Akuades Hidup Mati 30 0 30 0 30 0 30 0 Kontrol (+) Temephos 1ppm Hidup Mati 5 25 4 26 5 25 5 25 24 jam Konsentrasi Pengulangan 1 2 3 4 600 ppm Hidup Mati 30 0 30 0 30 0 30 0 700 ppm Hidup Mati 30 0 30 0 30 0 30 0 800 ppm Hidup Mati 30 0 30 0 30 0 30 0 Kontrol (-) 900 ppm Hidup Mati 30 0 30 0 30 0 30 0 Akuades Hidup Mati 30 0 30 0 30 0 30 0 Kontrol (+) Temephos 1ppm Hidup Mati 5 25 4 26 5 25 5 25 55 .

24 jam Pengulangan 1 2 3 1500 ppm Hidup Mati 0 30 0 30 0 30 Kontrol (-) Pengulangan 1 2 3 Akuades Hidup Mati 30 0 30 0 30 0 1700 ppm Hidup Mati 0 30 0 30 0 30 Konsentrasi 1900 ppm 2100 ppm Hidup Mati Hidup Mati 2 28 7 23 3 7 27 23 2 6 28 24 2300 ppm Hidup Mati 8 22 7 23 8 22 2500 ppm Hidup Mati 9 21 8 22 8 22 Kontrol (+) Temephos 1ppm Hidup Mati 5 25 4 26 5 25 56 .

1667 1.8187 83.0000 .18651 8.33 EEBP D3 4 99.67 20.2252 80.66667 .00 EEBP D4 4 99.2710 21.1944 40.67 100.33 83.5146 101.8187 96.83333 81.1809 73.66667 .1637 .3333 4.00 100.5146 101.67 Total 24 63.3956 16.30331 2. Deviation 95% Confidence Interval for Mean N Mean Std.00000 .5146 86.Lampiran 4 Descriptives Descriptives Persentase larva mati selama 24 jam Std.00 EEBP D2 4 78.83333 96.00000 .3333 1.33 86.00 Pembanding 4 84.83333 96. Error Lower Bound Upper Bound Minimum Maximum EEBP D1 4 18.00 57 .67 100.1667 1.0000 .66667 .96225 15.00 .8187 96.15166 71.1667 1.00 Kontrol 4 .4858 85.0000 .92450 .20304 46.

981 23 58 F 1455.667 18 5.Lampiran 5 Uji ANAVA satu arah ANOVA Persentase larva mati selama 24 jam Between Groups Within Groups Total Sum of Squares 37052.145 Sig.463 91.093 37143.315 df 5 Mean Square 7410.000 . .

000 75.2379 -94.16667 * .0712 5.9046 EEBP D2 5.000 -65.83333 * 1.000 -20.59571 1. Error Sig.0712 Kontrol 99.2621 EEBP D2 -78.59571 .4046 -73.00000 1.0712 5. The mean difference is significant at the .000 -70.000 -89.9046 EEBP D2 20.9046 -15.2379 Pembanding 15.9288 20.59571 .000 73.00000 * 1.59571 .83333 * 1.000 54.000 -104.33333 * 1.000 -83.9046 -15.83333 * 1.83333 * 1.000 13.4046 -13.0712 Kontrol 99.000 -85.59571 .9046 EEBP D3 Kontrol Pembanding Mean Difference (I-J) .83333 * 1.59571 .59571 .9046 -75.000 -23.9046 -75.33333 * 1.000 75.16667 * 1.59571 .000 9.000 -85.000 94.0712 -9.000 -25.83333 * 1.16667 * 1.000 15.2379 -94.59571 .000 60.7621 10.00000 1.0712 EEBP D1 -18.00000 * 1.59571 .00000 * 1.59571 .83333 * 1.59571 .9046 (I) Kelompok Perlakuan (J) Kelompok Perlakuan EEBP D1 EEBP D2 EEBP D3 EEBP D4 EEBP D2 EEBP D3 EEBP D4 EEBP D4 1.59571 .9288 20.00000 * 1.83333 * 1.2621 83.0954 104.019 .83333 * 1.0712 -9.000 79.05 level.59571 .9288 EEBP D4 -15.7621 85.Lampiran 6 Uji komparasi multipel Tukey HSD Multiple Comparisons Dependent Variable: Persentase larva mati selama 24 jam Tukey HSD 95% Confidence Interval Std.2621 EEBP D3 -99.7621 -80.59571 .59571 .7621 EEBP D1 80.4046 Pembanding -5.59571 .000 -5.33333 * 1.59571 .16667 * 1.9046 -60.000 -5.9288 Kontrol 84.59571 .7621 EEBP D1 60.0954 EEBP D4 -99.59571 .2379 Pembanding 15.83333 * 1. 59 .9046 EEBP D2 20.59571 .0954 104.7621 Kontrol 78.4046 Pembanding -65.83333 * 1.59571 .59571 .0712 EEBP D3 -20.000 15.7621 25.7621 25.00000 * 1.7621 EEBP D4 -20.59571 .59571 .9288 65.0954 EEBP D1 65.33333 * 1.000 -25.59571 .9288 -80.000 94.7621 70.83333 * 1.16667 * 1.59571 .0712 EEBP D1 80.2379 -79. Lower Bound Upper Bound -60.59571 .59571 1.7621 85.9046 EEBP D3 -15.019 -10.7621 Kontrol 18.00000 * 1.0954 Pembanding -84.0712 -54.000 9.9046 -.16667 * 1.000 -104.2379 *.000 -20.59571 .2621 23.0954 89.

a.000.0000 EEBP D1 4 EEBP D2 4 Pembanding 4 EEBP D3 4 EEBP D4 4 Sig.1667 99. 2 3 4 5 18.000 1. Uses Harmonic Mean Sample Size = 4.3333 84. 60 1.3333 78.000 1.05 Kelompok Perlakuan N 1 Kontrol 4 .1667 1.000 1.000 Means for groups in homogeneous subsets are displayed.1667 99.Lampiran 7 Homogeneous Subsets Persentase larva mati selama 24 jam a Tukey HSD Subset for alpha = .000 .

...........86470 1...........000 (PROBIT(p)) = Intercept + Regression Coeff..C * * * * * * * * * * * * * * * * * * * P R O B I T A N A L Y S I S * * * * * Parameter estimates converged after 16 iterations... 1 case is in the control group... .Lampiran 8 Analisis Probit * * * * * * * * * * * * * * * * * * * DATA P R O B I T A N A L Y S I S * * * * * Information 16 unweighted cases accepted.E......... MODEL Information ONLY Normal Sigmoid is requested..35299 3......00452 21.. Optimal solution found..207 DF = 14 P Since Goodness-of-Fit Chi square is significant.10425 -21. Parameter Estimates (PROBIT model: BX): Dosis = Pearson ... -67...69705 Goodness-of-Fit Chi Square = 219..E... Standard Error Coeff...76630 Intercept Standard Error Intercept/S.... 0 cases rejected because of missing data.. 0 cases rejected because LOG-transform can't be done.C 61 .. 21.......... .. a heterogeneity factor is used in the calculation of confidence limits......../S..

281 3.3 76.7 19.98175 .0 100.052 Prob .7 76.* * * * * * * * * * * * * * * * * * * P R O B I T A N A L Y S I S * * * * * Observed and Expected Frequencies Dosis Number of Subjects Observed Responses Expected Responses Residual 3.7 98.472 3.76805 .04 100.98175 .0 19.7 19.3 76.825 3.04 100.0 16.19659 .195 3.04 100.659 -2.0 20.0 76.052 3.11 100.0 96.0 98.19659 .805 -3.138 3.052 3.98175 .23 100.98175 .99948 .11 100.948 .341 3.7 99.76805 .0 100.0 16.175 1.0 99.23 100.0 98.0 100.18 100.0 73.805 -.825 3.18 100.948 .175 1.04 100.992 3.0 98.0 20.18 100.0 100.659 .508 3.18 100.0 80.76805 .0 19.948 .175 1.0 76.992 3.99948 C * * * * * * * * * * * * * * * * * * * P R O B I T A N A L Y S I S Confidence Limits for Effective Dosis Prob Dosis 95% Confidence Limits Lower Upper 62 * * * * * .0 100.805 6.0 96.0 100.341 3.19659 .0 99.23 100.0 83.805 3.11 100.23 100.0 99.948 -3.19659 .659 -2.659 .825 3.99948 .76805 .11 100.175 -1.528 3.99948 .

82839 1292.52443 1200.92260 1598.75 .29640 1108.03365 1342.55618 1662.80301 1181.95357 1372.09677 1256.24508 1000.04 .10 .55510 1333.82448 1364.02 .11539 1494.60 .08232 1537.38181 1318.92321 1082.60593 1352.72866 1574.95 .98 .18705 63 1023.06 .69634 1790.64027 1136.41855 1120.01 .74227 824.42614 987.80942 1379.62737 1521.17329 1113.15050 1467.90516 1102.04961 1536.63763 1171.03516 908.11114 1447.97 .24277 1506.39223 1626.01876 1051.09 .29126 1377.54872 1071.00617 1395.63652 897.03755 1253.98756 1238.09247 1295.66054 929.18989 1325.99194 969.36447 1035.14968 1218.08 .25 .51097 1239.20 .85237 1124.49593 1219..01847 1406.99 941.69411 1080.48785 1449.86512 1012.30 .53520 1399.91 .09061 1315.17038 1143.28378 1206.34071 1282.67636 1046.62348 1342.00979 1060.50 .94 .70 .04637 1710.42575 1405.84973 1095.40 .71696 1342.51448 938.52821 1236.96454 1045.92912 849.90299 1088.54963 1103.95610 1554.78152 1311.65339 977.33426 868.79356 .48459 1259.34767 1173.46767 1059.81074 1190.65 .05229 1101.05 .08244 1021.49487 1275.81105 1187.85 .96 .93281 1156.36804 1222.97591 1138.59538 787.07 .72912 1030.55455 1079.13584 1430.55 .80 .13947 1008.93 .10702 883.35 .39164 1386.26301 1037.85700 1417.91732 919.45 .67441 1203.92 .90 .33618 1271.32494 1317.15 .75202 1162.59926 1431.83482 1155.03 .

RIWAYAT HIDUP Nama : Wilma Angela NRP : 0510101 Alamat : Jl. Sukakarya 21 Bandung. Lulus SLTP Xaverius VI Palembang 2005. Mahasiswa Fakultas Kedokteran Universitas Kristen Maranatha Bandung 64 . Lulus SD Xaverius I Palembang 2002. Tempat/ Tanggal Lahir : Palembang/ 21April 1987 Riwayat Pendidikan : 1993. Lulus TK Xaverius I Palembang 1999. Lulus SMAK Kolese St. Yusuf Malang 2005.