The following show expected colony appearances and morphologies (shapes) of

Escherichia coli and Staphylococcus aureus. Note characteristics such as edges, color,
and whether the colonies are rough or smooth in texture.
For colony appearances of E. coli and S. aureus, scientists often describe what they
look like on agar. This is not the microscopic view (for example, as with a slide) but a
“naked eye” view of how the bacterial colonies look while growing on a medium. (This
is one type of culture.) If it is just plain nutrient agar (like below) … and it depends on
what kind of growth medium is used, the colors should be similar to the pictures below.
(In these examples, the time after plating is different for the two samples, so be aware
that the colonies in these photos are different sizes for a reason.) We will culture our
bacteria for about 2-7 days. Characteristics are most visible if the bacteria are
examined using a stereoscopic scope.

E. coli
Shape (form): circular
Margin: entire
Elevation: raised
Size: punctiform, small
Texture (surface): smooth
Appearance: shiny
Pigmentation: nonpigmented
(colorless)
Optical property: translucent

For all bacteria, they can be described
on the basis of these traits (how the
bacteria look) when grown on a medium,
poured and allowed to harden in a petri
dish. Characteristics of bacterial colonies
become one of the type of signatures;
keep in mind there are many ways that

S. aureus
Shape (form): circular
Margin: entire
Elevation: convex
Size: moderate, large
Texture (surface): rough
Appearance: shiny
Pigmentation: tan, golden yellow
Optical property: opaque

Its presence in water or food is an indication of fecal contamination. An Introduction. Members of this species can live in the presence or absence of oxygen and they can adapt to either type of situation. E. Here are how these particular bacteria are classified according to Bergy’s Manual of Systematic Bacteriology.bacteria can be identified. Funke. and certain strains produce endotoxins that cause traveler’s diarrhea and occasionally this type of bacteria can cause very serious foodborne disease. They grow comparatively well under conditions of dilute salt concentrations and low moisture. 11th edition (authors: Tortora. coli is not usually pathogenic. aureus is a common problem in hospitals. it can be the cause of urinary tract infections. aureus Domain: Phylum: Class: Order: Family: Genus: Species: Bacteria Firmicutes Bacilli Bacillales Staphylococcaceae Staphylococcus aureus Taken from Microbiology. (Remember there are 3 Domains and 5 Kingdoms of Life. However. The infection of surgical wounds by S. Staphylococcus aureus is named for its yellow-pigmented colonies (aureus = golden). and Case): Escherichi coli is one of the most common inhabitants of the human intestinal tract and is probably one of the most studied/familiar organisms in microbiology. . coli Domain: Phylum: Class: Order: Family: Genus: Species: Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriacae Escherichia coli S. which partially explains why they can grow and survive in nasal secretions and on the skin’s surface.) E. This is just one of the ways that bacteria can be explored. And its ability to develop resistance to such antibiotics as penicillin contributes to its danger to patients in hospital environments.