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Name: KAMARAJ MICHAEL RAJKUMAR

Exercise 1: Cell Transport Mechanisms and Permeability: Activity 1: Simulating Dialysis (Simple Diffusion) Lab Report
Pre-lab Quiz Results
You scored 75% by answering 3 out of 4 questions correctly.
1. The driving force for diffusion is
You correctly answered: b. the kinetic energy of the molecules in motion.
2. In diffusion, molecules move
You correctly answered: a. from high concentration to low concentration.
3. Which of the following dialysis membranes has the largest pore size?
You correctly answered: d. 200 MWCO
4. Avogadro's number is a constant for the number of
Your answer : a. moles.
Correct answer: b. molecules.

08/24/14

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00 0.0094 .0000 0.00 0.00 0.00 9.00 0. Predict Question 2: Recall that glucose is a monosaccharide.00 0.00 0.0000 0.0150 0. Which of the following will be able to diffuse through the 200 MWCO membrane? Your answer : c.00 0.00 0.00 0.00 0.0000 0. glucose only Stop & Think Questions: The reason sodium chloride didn't diffuse left to right is that You correctly answered: c.0000 0. No.00 0. Glucose is a six-carbon sugar. and the average molecular weight of a single amino acid is 135 g/mole.00 0.00 0.00 0.0273 0.0000 0. the membrane pore size was too small.0000 0. Do you think urea will diffuse through the 20 MWCO membrane? Your answer : c.00 0. Albumin is a protein with 607 amino acids.00 18.00 0. The average molecular weight of a single amino acid is 135 g/mole.0000 0.0000 0.07.00 0.00 0.00 0.00 08/24/14 page 2 Average Diffusion Rate 0.00 0. is slower than that for sodium because urea is a larger molecule.00 0.00 0. There is no reason to run these solutes at the 20 MWCO because You correctly answered: d.00 0.0000 0.0000 0.00 0.00 0.00 0.00 0.0000 0.0000 0. Experiment Data: Solute MWCO Left Solute Concentration Right Solute Concentration Na+ ClUrea Albumin Glucose Na+ ClUrea Albumin Glucose Na+ ClUrea Albumin Glucose Na+ ClUrea Albumin Glucose Na+ ClUrea Albumin Glucose Na+ ClUrea 20 20 20 20 20 20 20 20 50 50 50 50 50 50 50 50 100 100 100 100 100 100 9.0000 0.0000 0.00 0.0000 0.00 0.0000 0.00 0. albumin is a protein with 607 amino acids.00 0. The rate of diffusion for urea You correctly answered: b. glucose and albumin are both too large to pass.00 9.00 9.00 0.0150 0.00 0.00 0.00 0.00 0. not at all.00 9.0000 0.Experiment Results Predict Question: Predict Question 1: The molecular weight of urea is 60.00 0.0000 0.00 0.

00 0.00 9.00 0.0000 0.00 0.0000 0.Solute MWCO Left Solute Concentration Right Solute Concentration Albumin Glucose Na+ ClUrea Albumin Glucose Na+ ClUrea Albumin Glucose 100 100 200 200 200 200 200 200 200 200 0.00 0.00 0.0000 0.00 0.0000 0.00 08/24/14 page 3 Average Diffusion Rate 0.00 0.0042 0.00 0.00 0.00 0.00 0.00 0.0000 0.00 9.0000 0.0000 .00 0.0000 0.00 0.00 0.00 0.00 0.0000 0.

we say the solution has reached You correctly answered: a. Describe the difference between the rate of diffusion seen for sodium and urea. 08/24/14 page 4 . You correctly answered: c. Which of the following solutes did not pass through any of the membranes? You correctly answered: b. 3. equilibrium. albumin 4. When diffusion stops. The effect of increasing the concentration of sodium chloride from 9 mM to 18 mM in the left beaker was to You correctly answered: b. 2. Urea diffused more slowly because it is larger than sodium. increase the rate of diffusion.Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1.

and urea. urea. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. glucose.molecular weight cut off 2. Put the following in order from smallest to largest molecular weight: glucose. Describe two variables that affect the rate of diffusion. How well did the results compare with your prediction? Your answer: nothing passes as glucose and albumin are larger molecules 4. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? Your answer: The size of urea is larger than the size of pores in 20 MWCO 3.Review Sheet Results 1. albumin. albumin 08/24/14 page 5 . Your answer: sodium chloride.molecular weight 2. Your answer: 1. sodium chloride.