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R05

Code No: R05422301

Set No. 2

IV B.Tech II Semester Examinations,JULY 2011


ANIMAL CELL SCIENCE AND TECHNOLOGY
Bio-Technology
Time: 3 hours
Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
?????
1. Explain in detail the suspension cloning.

[16]

2. Describe the essential features of media employed in:


(a) CO2 - gassed incubators.

D
L

(b) Non-gassed incubators.

3. What are somatic progenitor cells and normal tissue renewal?

R
O

[8+8]
[16]

4. Describe the procedure and method of calculations in detail on counting the cells
by employing hemocytometer.
[16]
5. What are bioengineered ocular surface equivalents? Explain the details of its applications.
[16]

W
U

6. Explain the protocol used to develop spheroids.

[16]

7. What are the best practices, with reference to the following, while working in the
cell culture lab:

T
N

(a) Culture hood area.

(b) Handling and operation of pipettes & Pipette tips.


(c) Aliquoting sterile medium.
(d) Handling of syringe filter units.

[44]

8. Describe any two methods of measurement of apoptosis in cell cultures.


?????

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[16]

www.jntuworld.com

R05

Code No: R05422301

Set No. 4

IV B.Tech II Semester Examinations,JULY 2011


ANIMAL CELL SCIENCE AND TECHNOLOGY
Bio-Technology
Time: 3 hours
Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
?????
1. What are somatic progenitor cells and normal tissue renewal?

[16]

2. Describe the essential features of media employed in:


(a) CO2 - gassed incubators.

D
L

(b) Non-gassed incubators.


3. Explain the protocol used to develop spheroids.

R
O

[8+8]
[16]

4. Describe the procedure and method of calculations in detail on counting the cells
by employing hemocytometer.
[16]
5. What are bioengineered ocular surface equivalents? Explain the details of its applications.
[16]

W
U

6. What are the best practices, with reference to the following, while working in the
cell culture lab:

T
N

(a) Culture hood area.

(b) Handling and operation of pipettes & Pipette tips.

(c) Aliquoting sterile medium.


(d) Handling of syringe filter units.

[44]

7. Explain in detail the suspension cloning.


8. Describe any two methods of measurement of apoptosis in cell cultures.
?????

www.jntuworld.com

[16]
[16]

www.jntuworld.com

R05

Code No: R05422301

Set No. 1

IV B.Tech II Semester Examinations,JULY 2011


ANIMAL CELL SCIENCE AND TECHNOLOGY
Bio-Technology
Time: 3 hours
Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
?????
1. Describe any two methods of measurement of apoptosis in cell cultures.

[16]

2. Describe the procedure and method of calculations in detail on counting the cells
by employing hemocytometer.
[16]

D
L

3. What are bioengineered ocular surface equivalents? Explain the details of its applications.
[16]

R
O

4. Describe the essential features of media employed in:


(a) CO2 - gassed incubators.
(b) Non-gassed incubators.

W
U

5. What are somatic progenitor cells and normal tissue renewal?

[8+8]
[16]

6. What are the best practices, with reference to the following, while working in the
cell culture lab:

T
N

(a) Culture hood area.

(b) Handling and operation of pipettes & Pipette tips.

(c) Aliquoting sterile medium.


(d) Handling of syringe filter units.

[44]

7. Explain in detail the suspension cloning.


8. Explain the protocol used to develop spheroids.
?????

www.jntuworld.com

[16]
[16]

www.jntuworld.com

R05

Code No: R05422301

Set No. 3

IV B.Tech II Semester Examinations,JULY 2011


ANIMAL CELL SCIENCE AND TECHNOLOGY
Bio-Technology
Time: 3 hours
Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
?????
1. Describe the essential features of media employed in:
(a) CO2 - gassed incubators.
(b) Non-gassed incubators.

D
L

[8+8]

2. What are the best practices, with reference to the following, while working in the
cell culture lab:

R
O

(a) Culture hood area.

(b) Handling and operation of pipettes & Pipette tips.


(c) Aliquoting sterile medium.

W
U

(d) Handling of syringe filter units.

3. What are somatic progenitor cells and normal tissue renewal?

T
N

[44]
[16]

4. Explain the protocol used to develop spheroids.

[16]

5. Describe any two methods of measurement of apoptosis in cell cultures.

[16]

6. Describe the procedure and method of calculations in detail on counting the cells
by employing hemocytometer.
[16]
7. Explain in detail the suspension cloning.

[16]

8. What are bioengineered ocular surface equivalents? Explain the details of its applications.
[16]
?????

www.jntuworld.com